Journal of Forest and Environmental Science

Institute of Forest Science, kangwon National University (강원대학교 산림과학연구소)
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In vitro Organogenesis and Propagation of Heloniopsis orientalis Thunb

  • Jong-Woo Nam (Department of Applied Biology, Kangwon National University) ;
  • Yoon-Kyung Choi (Seedvault Center, Baekdudaegan National Arboretum) ;
  • Kyeong-Mi Cho (Seedvault Center, Baekdudaegan National Arboretum) ;
  • Young-Been Kim (Seedvault Center, Baekdudaegan National Arboretum) ;
  • Sung Hwan Yim (Department of Biology, Kyungpook National University) ;
  • Kee Hwa Bae (Seedvault Center, Baekdudaegan National Arboretum)
  • Received : 2023.05.31
  • Accepted : 2023.06.23
  • Published : 2023.09.30
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Abstract

Heloniopsis orientalis (Liliaceae) is an important horticultural crop native to Korea. Under natural conditions, germination is poor and plant growth is delayed. Therefore, we have developed a vegetative propagation method to produce plants with vigorous growth characteristics via tissue culture. The regenerated shoots were then initiated directly from leaf explants on an MS medium containing either 0.5 to 2.0 mg/L 2,4-D or 1.0 to 3.0 mg/L BA. Healthy plantlets with adventitious roots were formed on the medium supplemented with 1.0 mg/L BA (81%). BA triggered callus initiation without caulogenesis or rhizogenesis, and callus formation was better on the half-strength MS medium than on the full-strength medium. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation.

Keywords

Acknowledgement

This study was carried out with the support of R&D Program for Forest Science Technology (Project No. 2021398B10-2325-CA02) provided by Korea Forest Service (Korea Forestry Promotion Institute).

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