The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Sweroside plays a role in mitigating high glucose-induced damage in human renal tubular epithelial HK-2 cells by regulating the SIRT1/NF-κB signaling pathway

  • Xiaodan Ma (Department of Endocrinology, Second Hospital, Shanxi Medical University) ;
  • Zhixin Guo (Department of Endocrinology, Second Hospital, Shanxi Medical University) ;
  • Wenhua Zhao (Department of Endocrinology, Changzhi People's Hospital Affiliated to Changzhi Medical College) ;
  • Li Chen (Department of Endocrinology, Changzhi People's Hospital Affiliated to Changzhi Medical College)
  • Received : 2023.05.23
  • Accepted : 2023.08.10
  • Published : 2023.11.01
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Abstract

Sweroside is a natural monoterpene derived from Swertia pseudochinensis Hara. Recently, studies have shown that sweroside exhibits a variety of biological activities, such as anti-inflammatory, antioxidant, and hypoglycemic effects. However, its role and mechanisms in high glucose (HG)-induced renal injury remain unclear. Herein, we established a renal injury model in vitro by inducing human renal tubular epithelial cell (HK-2 cells) injury by HG. Then, the effects of sweroside on HK-2 cell activity, inflammation, reactive oxygen species (ROS) production, and epithelial mesenchymal transition (EMT) were observed. As a result, sweroside treatment ameliorated the viability, inhibited the secretion of inflammatory cytokines (TNF-α, IL-1β, and VCAM-1), reduced the generation of ROS, and inhibited EMT in HK-2 cells. Moreover, the protein expression of SIRT1 was increased and the acetylation of p65 NF-kB was decreased in HK-2 cells with sweroside treatment. More importantly, EX527, an inhibitor of SIRT1, that inactivated SIRT1, abolished the improvement effects of sweroside on HK-2 cells. Our findings suggested that sweroside may mitigate HG-caused injury in HK-2 cells by promoting SIRT1-mediated deacetylation of p65 NF-kB.

Keywords

Acknowledgement

This work was supported by the Shanxi Scholarship Council of China (Grant No. 2020-192) and the natural science foundation of Shanxi Province of China (Grant No. 202103021224414).

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