Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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A Study on the Antioxidant and Anti-inflammatory Effects of Hamyang Wild-Simulated Ginseng and Ginseng Berry Complex

함양산양삼과 열매 복합물의 항산화 및 항염증 효능에 대한 연구

  • Jong-Sang Lee (Department of Pathology, College of Korean Medicine, Daejeon University) ;
  • In-Hwan Joo (Department of Pathology, College of Korean Medicine, Daejeon University) ;
  • Jong-Min Park (Department of Pathology, College of Korean Medicine, Daejeon University) ;
  • Dong-Hee Kim (Department of Pathology, College of Korean Medicine, Daejeon University)
  • 이종상 (대전대학교 한의과대학 병리학교실) ;
  • 주인환 (대전대학교 한의과대학 병리학교실) ;
  • 박종민 (대전대학교 한의과대학 병리학교실) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Received : 2023.04.19
  • Accepted : 2023.06.19
  • Published : 2023.06.25
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Abstract

The purpose of this study is to examine the antioxidant effect of hamyang wild-simulated ginseng and ginseng berry complex(HGC) and to investigate the mechanism of anti-inflammatory effect on macrophage cell line (RAW264.7 cells). For antioxidant activity of HGC was measured by total polyphenol contents were estimated with folin-ciocalteu method, and total flavonoid contents were estimated with aluminum chloride methods. And, assessed with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity assay. MTT assay was performed to determine the effect of HGC on cell viability in RAW264.7 cells. In addition, anti-inflammatory effect of HGC was investigated in RAW264.7 cells. RAW 264.7 cells, in which an inflammatory response was induced by lipopolysarccharide (LPS), were treated with HGC, and nitric oxide production was measured by griess assay. And protein expression level of inflammatory cytokine and phosphorylation of MAPK signaling pathways were confirmed by western blot analysis. Total polyphenols and flavonoids contents in HGC were quantified, and increased DPPH and ABTS radical scavenging activity in a concentration-dependent manner was confirmed. In addition, HGC was significantly inhibited nitric oxide (NO) production and inflammatory cytokine (iNOS, IL-1β, IL-6, and TNF-α) expression in LPS-stimulated RAW264.7 cells. Also, HGC was significantly inhibited the phosphorylation of MAPK signaling pathways. These results suggest that HGC can be used as effective herbal medicine extract for antioxidant and anti-inflammatory effect. As a result, it is thought that HCG can be used as a functional material effective for antioxidant and anti-inflammatory effects. And it will be possible to promote industrialization using hamyang wild-simulated ginseng and ginseng berry, and to increase high-value-added.

Keywords

Acknowledgement

본 연구는 산림청(한국임업진흥원) 산림과학기술 연구개발사업(2021370A00-2123-BD02)의 지원에 의하여 이루어진 것입니다.

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