Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Rutin alleviated lipopolysaccharide-induced damage in goat rumen epithelial cells

  • Jinshun Zhan (Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science) ;
  • Zhiyong Gu (Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science) ;
  • Haibo Wang (Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science) ;
  • Yuhang Liu (Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science) ;
  • Yanping Wu (Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science) ;
  • Junhong Huo (Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Science)
  • Received : 2023.01.31
  • Accepted : 2023.09.04
  • Published : 2024.02.01
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Abstract

Objective: Rutin, also called vitamin P, is a flavonoids from plants. Previous studies have indicated that rutin can alleviate the injury of tissues and cells by inhibiting oxidative stress and ameliorating inflammation. There is no report on the protective effects of rutin on goat rumen epithelial cells (GRECs) at present. Hence, we investigated whether rutin can alleviate lipopolysaccharide (LPS)-induced damage in GRECs. Methods: GRECs were cultured in basal medium or basal medium containing 1 ㎍/mL LPS, or 1 ㎍/mL LPS and 20 ㎍/mL rutin. Six replicates were performed for each group. After 3-h culture, the GRECs were harvested to detect the relevant parameters. Results: Rutin significantly enhanced the cell activity (p<0.05) and transepithelial electrical resistance (TEER) (p<0.01) and significantly reduced the apoptosis rate (p<0.05) of LPS-induced GRECs. Rutin significantly increased superoxide dismutase, glutathione peroxidase, and catalase activity (p<0.01) and significantly decreased lactate dehydrogenase activity and reactive oxygen species and malondialdehyde (MDA) levels in LPS-induced GRECs (p<0.01). The mRNA and protein levels of interleukin 6 (IL-6), IL-1β, and C-X-C motif chemokine ligand 8 (CXCL8) and the mRNA level of tumor necrosis factor-α (TNF-α) and chemokine C-C motif ligand 5 (CCL5) were significantly increased in LPS-induced GRECs (p<0.05 or p<0.01), while rutin supplementation significantly decreased the mRNA and protein levels of IL-6, TNF-α, and CXCL8 in LPS-induced GRECs (p<0.05 or p<0.01). The mRNA level of toll-like receptor 2 (TLR2), and the mRNA and protein levels of TLR4 and nuclear factor κB (NF-κB) was significantly improved in LPS-induced GRECs (p<0.05 or p<0.01), whereas rutin supplementation could significantly reduce the mRNA and protein levels of TLR4 (p<0.05 or p<0.01). In addition, rutin had a tendency of decreasing the protein levels of CXCL6, NF-κB, and inhibitor of nuclear factor kappa-B alpha (0.05

Keywords

Acknowledgement

This study was supported by the Earmarked Fund for Jiangxi Agriculture Research System (JXARS-13) and the Collaborative Innovation Special Project of Jiangxi Modern Agricultural Scientific Research (JXXTCXQN202203).

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