The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Mechanism of Wenshen Xuanbi Decoction in the treatment of osteoarthritis based on network pharmacology and experimental verification

  • Hankun You (Department of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine) ;
  • Siyuan Song (Department of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine) ;
  • Deren Liu (Department of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine) ;
  • Tongsen Ren (Department of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine) ;
  • Song Jiang Yin (Department of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine) ;
  • Peng Wu (Department of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine) ;
  • Jun Mao (Department of Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine)
  • Received : 2023.10.18
  • Accepted : 2023.11.14
  • Published : 2024.01.01
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Abstract

To investigate the mechanism of Wenshen Xuanbi Decoction (WSXB) in treating osteoarthritis (OA) via network pharmacology, bioinformatics analysis, and experimental verification. The active components and prediction targets of WSXB were obtained from the TCMSP database and Swiss Target Prediction website, respectively. OA-related genes were retrieved from GeneCards and OMIM databases. Protein-protein interaction and functional enrichment analyses were performed, resulting in the construction of the Herb-Component-Target network. In addition, differential genes of OA were obtained from the GEO database to verify the potential mechanism of WSXB in OA treatment. Subsequently, potential active components were subjected to molecular verification with the hub targets. Finally, we selected the most crucial hub targets and pathways for experimental verification in vitro. The active components in the study included quercetin, linolenic acid, methyl linoleate, isobergapten, and beta-sitosterol. AKT1, tumor necrosis factor (TNF), interleukin (IL)-6, GAPDH, and CTNNB1 were identified as the most crucial hub targets. Molecular docking revealed that the active components and hub targets exhibited strong binding energy. Experimental verification demonstrated that the mRNA and protein expression levels of IL-6, IL-17, and TNF in the WSXB group were lower than those in the KOA group (p < 0.05). WSXB exhibits a chondroprotective effect on OA and delays disease progression. The mechanism is potentially related to the suppression of IL-17 and TNF signaling pathways and the down-regulation of IL-6.

Keywords

Acknowledgement

The third batch of peak academic talent project of Jiangsu Provincial Hospital of Traditional Chinese Medicine (second level) (No.: y2021rc20).

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