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The Korean Society of Phycology (한국조류학회(藻類))
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Lipidomic analysis exposes the role of lipids in the maturation of conchocelis of Pyropia haitanensis (Bangiales, Rhodophyta)

  • Lin Liu (Fisheries College, Jimei University) ;
  • Yinghui Lin (Fisheries College, Jimei University) ;
  • Yan Xu (Fisheries College, Jimei University) ;
  • Dehua Ji (Fisheries College, Jimei University) ;
  • Chaotian Xie (Fisheries College, Jimei University) ;
  • Wenlei Wang (Fisheries College, Jimei University)
  • Received : 2025.04.24
  • Accepted : 2025.12.01
  • Published : 2025.12.15
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Abstract

Due to the fact that Pyropia haitanensis contains numerous nutritional and biofunctional compounds, its development prospects are very promising. However, asynchronous conchocelis development has limited the large-scale use of new varieties of P. haitanensis. In this study, we combined lipid metabolomic and transcriptomic data to analyze the free-living conchocelis of two P. haitanensis strains that vary regarding conchocelis maturation at specific time points to investigate the mechanism underlying conchocelis maturation. Phosphatidylcholine (PC) synthesis was found to be closely related to the initiation of the maturation process, and genes associated with triacylglycerol (TG) accumulation coincided with a relative decrease in polyunsaturated membrane lipids, consistent with a buffering/energy-reserve role during maturation. PC abundance increased early in early-maturing strain (S1), and higher TG levels coincided with lower polyunsaturated membrane lipids, suggesting a potential buffering mechanism against environmental stimuli during maturation. The accumulated TG can be metabolized to produce energy to enhance the maturation of free-living conchocelis. Moreover, free-living conchocelis can increase the digalactosyldiglyceride/monogalactosyldiglyceride ratio to adapt to maturation conditions. Additionally, differences between the S1 and late-maturing strain (S2) during the conchocelis maturation process were clarified. For example, S1 initiates PC synthesis and metabolism earlier than S2, while also producing arachidonic acid relatively quickly to improve membrane fluidity. Furthermore, S1 metabolizes TG faster than S2 to provide energy and maintain lipid homeostasis, thereby promoting free-living conchocelis maturation. In summary, lipid metabolism, particularly phospholipid metabolism, plays a crucial role in initiating the conchocelis maturation process and facilitating the formation of conchosporangia.

Keywords

Acknowledgement

This research was supported by the National Natural Science Foundation of China (grant number 32473157), the Outstanding Natural Science Foundation Project of Fujian Province (grant number 2022J06024), the Science and Technology Project for Regional Development in Fujian Province (grant number 2023N3002), the Research on Industrial InnovationTechnology for Guangdong Modern Marine Ranching (2024-MRI-001) and the China Agriculture Research System of MOF and MARA (grant number CARS-50). We thank Liwen Bianji (Edanz) (www.liwenbianji.cn) for editing the English text of a draft of this manuscript.

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