When a wind turbine is designed, the dynamic stability of the system as well as the dynamic characteristics of the main components such as blades, hub, main shaft and tower must be evaluated. In particular, the natural frequencies of a blade, as a main load-generating component, need to be measured and assessed by component level testing. In conventional practice, the natural frequencies of a blade are determined as the measured frequencies near the reference frequencies provided by FE analysis results. But the reference frequencies are also uncertain since designers have difficulty distinguishing the torsional mode shape among the analysis results due to the complexity of its mode shape. So, in conventional practice, the determination of a measured torsional frequency inevitably contains uncertainty. Therefore, a novel method to definitely determine the torsional frequencies from the experimental data itself is necessary. In this paper, a new methodology to measure the torsional frequency of a blade was studied from the perspective of a modal test procedure, data processing method and mode determination logic. Finally, the validity of the method that can measure torsional frequency without reference FE analysis results was verified by applying it to an actual large wind turbine blade