• 한국초전도ㆍ저온공학회논문지
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• 제24권1호
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• pp.18-22
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• 2022

The effect of electron beam (EB) irradiation on superconducting properties and microstructures of MgB₂ bulk superconductors were investigated. At E-beam doses of 1×10¹⁶ e/cm² and 1×10¹⁷ e/cm², the effect of irradiation on a superconducting transition temperature (T_c) of MgB₂ was weak. As a dose increases to 5×10¹⁷ e/cm², T_c decreases by 0.5 K. The critical current density (J_c) measured at 4.2 K and 20 K, and 0 T - 5 T increases slightly as exposure time increases. X-ray diffraction for the irradiation surface of MgB₂ shows that the diffraction intensity of (hkl) peaks decreases proportionally as the exposure time increases. This indicates that the crystallinity of MgB₂ was degraded by irradiation. TEM investigation for the irradiated sample showed distorted lattice structure, which is consistent with the XRD results. The J_c increase and T_c reduction of MgB₂ by irradiation are believed to be caused by the lattice distortion.

• Bulletin of the Korean Chemical Society
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• 제24권8호
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• pp.1181-1187
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• 2003

Condensation of 4-cyano-5,6-dimethyl-3-pyridazinone 1 with aromatic aldehydes gave the novel styryl derivatives 2a-c. Refluxing of compound 2a with phosphorus oxychloride furnished 3-chloropyridazine derivative 3. Compound 3 was reacted with thiourea and produced pyridazine-3(2H)thione 4. Thieno[2,3-c]- pyridazines 5a-e were achieved by cycloalkylation of compound 4 with halocompounds in methanol under reflux and in the presence of sodium methoxide. Also, refluxing of compound 4 with N-substituted chloroacetamide in the presence of potassium carbonate afforded thienopyridazines 6a-e. Cyclization of compound 6 with some electrophilic reagents as carbon disulfide and triethyl orthoformate furnished the novel pyrimido[4',5':4,5]thieno[2,3-c]pyridazines 12 and 13a-c, respectively. Diazotisation of compound 6 with sodium nitrite in acetic acid produced the pyridazino[4',3':4,5]thieno[3,2-d][1,2,3]triazines 14a-c. Ternary condensation of compound 1, aromatic aldehydes and malononitrile in ethanol containing piperidine under reflux afforded the novel phthalazines 16a-c. Compound 3 was subjected to some nucleophilic substitution reactions with amines and sodium azide and formed the aminopyridazines 17a, b and tetrazolo[1,5-b]-pyridazine 19, respectively. The structures of the synthesized compounds were established by elemental and spectral analyses.

• ETRI Journal
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• 제42권5호
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• pp.781-789
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• 2020

In this study, an E-band low-noise amplifier (LNA) monolithic microwave integrated circuit (MMIC) has been designed using silicon-germanium 130-nm bipolar complementary metal-oxide-semiconductor technology to suppress unwanted signal gain outside operating frequencies and improve the signal gain and noise figures at operating frequencies. The proposed impedance-controllable filter has series (R_s) and parallel (R_p) resistors instead of a conventional inductor-capacitor (L-C) filter without any resistor in an interstage matching circuit. Using the impedance-controllable filter instead of the conventional L-C filter, the unwanted high signal gains of the designed E-band LNA at frequencies of 54 GHz to 57 GHz are suppressed by 8 dB to 12 dB from 24 dB to 26 dB to 12 dB to 18 dB. The small-signal gain S₂₁ at the operating frequencies of 70 GHz to 95 GHz are only decreased by 1.4 dB to 2.4 dB from 21.6 dB to 25.4 dB to 19.2 dB to 24.0 dB. The fabricated E-band LNA MMIC with the proposed filter has a measured S₂₁ of 16 dB to 21 dB, input matching (S₁₁) of -14 dB to -5 dB, and output matching (S₂₂) of -19 dB to -4 dB at E-band operating frequencies of 70 GHz to 95 GHz.

• Pediatric Infection and Vaccine
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• 제12권2호
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• pp.166-177
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• 2005

목 적 : DNA 제한 효소 분석법을 이용한 아데노바이러스의 유전체형 분석 방법은 많은 연구자들이 서로 다른 종류의 제한 효소와 명명법을 사용하여 분류함으로써, 아직까지 체계적인 분류 체계가 정립되어 있지 않다. 본 연구는 Li와 Wadell이 제안한 제한 효소 분석법과 명명법을 이용하여 국내에서 최근 14년 동안 분리된 아데노바이러스 혈청형 1, 2, 5형에 대한 유전체형을 분류하고, 그 분자 역학과 유전체형 상호 간의 연관성을 밝히고자 시행하였다. 방 법 : 1990년 11월부터 2003년 2월까지 하기도 감염으로 서울대학교병원 소아과에 입원하였거나, 인접 지역의 종합병원 소아과에 입원한 소아들로부터 채취한 비인두 흡인물을 검체로 하여 HEp-2 세포주에서 배양 후 간접면역형광검사로 확인하고, 분리된 아데노바이러스를 항혈청 1, 2, 3, 4, 5, 6, 7, 11에 대한 세포독성 효과를 관찰함으로 혈청형을 결정하였다. 혈철형 1, 2, 5형을 대상으로 DNA를 추출하고 12가지 제한 효소 BamHI, BcI, BglI, BglII, BstEII, EcoRI, HindIII, HpaI, SalI, SmaI, XbaI, XhoI로 절단한 후 전기영동 시키고 나타나는 분절 형태를 각 혈청형의 표준주와 비교하여 분석하였다. 유전체형간의 상관성을 비교하기 위하여 PCRF 분석을 시행하였다. 결 과 : 아데노바이러스 분리주 382주를 대상으로 혈청형을 확인한 결과, 1형 33(9%), 2형 45(12%), 3형 107(28%), 4형 16(4%), 5형 24(6%), 6형 8(2%), 7형 116(30%), 11형 9(2%), 그 외의 형들이 24(6%)주로 각각 나타났다. 변이 유전체형은 혈청형 1형 18종류, 2형 25종류, 5형 10종류가 분류되었으며 Ad1p1-Ad1p7, Ad1a, Ad1b, Ad1b1-Ad1b3, Ad1c, Ad1d, Ad1e, Ad1e1, Ad1e2, Ad1f; Ad2p1-Ad2p11, Ad2a, Ad2a1-Ad2a6, Ad2b, Ad2c, Ad2d, Ad2e, Ad2e1-Ad2e3; Ad5p1, Ad5p2, Ad5a, Ad5a1-Ad5a7로 명명되었다. 본 연구에서는 표준주나 이전에 보고 된 유전체형과 일치하는 유전체형은 분리되지 않았다. 대부분의 유전체형은 전 연구기간 동안 1~2주만 분리되었고 일부 유전체형들은 산발적으로 2회 이상 반복 분리되었다. 유전체형 Ad1p5나 Ad5a1와 같이 유행성으로 분포하는 유전체형도 관찰되었다. 혈청형 1형 유전체형 간의 PCRF는 79~99%로 Genomic cluster 1과 2로 구분되었고, 2형과 5형은 각각 82~99%와 84~99%로 모두 80% 이상이었다. 결 론 : 본 연구를 통하여 국내에서 분리된 아데노바이러스 혈청형 1, 2, 5형의 다양한 유전체형을 체계적으로 분석할 수 있었다. 혈청형 1, 2, 5형이 주로 산발성 감염을 일으키는 것으로 알려져 왔으나, 유전체형에 따라 역학적 특징이 다르게 나타날 수 있으며, 이는 DNA의 변형에 의한 유전체형의 변화가 바이러스의 감염력과 생존력에 변화를 일으켰을 가능성을 시사한다. 본 연구 결과는 혈청형 1, 2, 5형의 비교 자료로 활용될 수 있을 뿐만 아니라, 변이 유전체형들에 대한 정보를 제공함으로써 백신 개발을 위한 기초 자료로 활용될 수 있을 것으로 기대된다.

• 대한소아치과학회지
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• 제8권1호
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• pp.89-102
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• 1981

1147 preschool children aged from 3 to 6 living in some farm villages in Kyungki-do were surveyed on dmf and distribution of dental caries in primary dentition. The results were as follows: 1. dmf rate ... 92.76 % boy ... 94.52 % girl ... 90.5.5 % 2. dmf t rate ... 37.07 % dmf t index ... 7.09 3. dmf s rate ... 13.74 % dmf s index ... 13.14 % 4. df t index ... 6.94 5. Each percentage of d, m & f per dmf teeth d rate ... 89.96 % m rate ... 7.24 % f rate ... 2.67 % 6. Most frequent site of dental caries in each tooth $\underline{A}$ mesial $\overline{A}$ distal $\underline{B}$ mesial $\overline{B}$ distal $\underline{C}$ distal $\overline{C}$ distal $\underline{D}$ disto-occlusal $\overline{D}$ disto-occlusal $\underline{E}$ linguo-occlusal $\overline{E}$ occlusal 7. Order of caries susceptibility $\overline{E}$ $\underline{E}$ $\overline{D}$ $\underline{D}$ $\underline{A}$ $\underline{B}$ $\overline{C}$ $\underline{C}$ $\overline{B}$ $\overline{A}$ 8. All the values in caries criteria showed a marked increae from those of 1968.

• Clinical and Experimental Reproductive Medicine
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• 제26권2호
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• pp.163-170
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• 1999

Objectives: The aims of this study are 1) to determine if GAST is a better indicator in predicting ovarian response to COH compared with patient's age or basal FSH level and 2) to evaluate its role in detecting abnormal ovarian response. Design: Prospective study in 118 patients undergoing IVF-ET using GnRH-a short protocol during May-September 1995. Materials and Methods: After blood sampling for basal FSH and estradiol $(E_2)$ on cycle day two, 0.5ml (0.525mg) GnRH agonist ($Suprefact^{(r)}$, Hoechst) was injected subcutaneously. Serum $E_2$ was measured 24 hours later. Initial $E_2$ difference $({\Delta}E_2)$ was defined as the change in $E_2$ on day 3 over the baseline day 2 value. Sixteen patients with ovarian cyst or single ovary or incorrect blood collection time were excluded from the analysis. The patients were divided into three groups by ${\Delta}E_2$; group A (n=30):${\Delta}E_2$<40 pg/ml, group B (n=52): 40 pg/ml${\leq}{\Delta}E_2$<100 pg/ml, group C (n=20): ${\Delta}E_2{\leq}100$ pg/ml. COH was done by GnRH agonist/HMG/hCG and IVF-ET was followed. Ratio of $E_2$ on day of hCG injection over the number of ampules of gonadotropins used ($E_2hCGday$/Amp) was regarded as ovarian responsiveness. Poor ovarian response and overstimulation were defined as $E_2$ hCGday less than 600 pg/ml and greater than 5000 pg/ml, respectively. Results: Mean age $({\pm}SEM)$ in group A, B and C were $33.7{\pm}0.8^*,\;31.5{\pm}0.6\;and\;30.6{\pm}0.5^*$, respectively ($^*$: p<0.05). Mean basal FSH level of group $A(11.1{\pm}1.1mlU/ml)$ was significantly higher than those of $B(7.4{\pm}0.2mIU/ml)$ and C $(6.8{\pm}0.4mIU/ml)$ (p<0.001). Mean $E_2hCGday$ of group A was significantly lower than those of group B or C, i.e., $1402.1{\pm}187.7pg/ml,\;3153.2{\pm}240.0pg/ml,\;4078.8{\pm}306.4pg/ml$ respectively (p<0.0001). The number of ampules of gonadotropins used in group A was significantly greater than those in group B or C: $38.6{\pm}2.3,\;24.2{\pm}1.1\;and\;18.5{\pm}1.0$ (p<0.0001). The number of oocytes retrieved in group A was significantly smaller than those in group B or C: $6.4{\pm}1.1,\;15.5{\pm}1.1\;and\;18.6{\pm}1.6$, respectively (p<0.0001). By stepwise multiple regression, only ${\Delta}E_2$ showed a significant correlation (r=0.68, p<0.0001) with $E_2HCGday$/Amp, while age or basal FSH level were not significant. Likewise, only ${\Delta}E_2$ correlated significantly with the number of oocytes retrieved (r=0.57, p<0.001). All four patients whose COH was canceled due to poor ovarian response belonged to group A only (Fisher's exact test, p<0.01). Whereas none of 30 patients in group A (0%) had overstimulation, 14 patients among 72 patients (19.4%) in group B and C had overstimulation (Fisher's exact test, p<0.01). Conclusions: These data suggest that initial $E_2$ difference after GAST may be a better prognostic indicator of ovarian response to COH than age or basal FSH level. Since initial $E_2$ difference demonstrates significant association with abnormal ovarian response such as poor ovarian response necessitating cycle cancellation or overstimulation, GAST may be helpful in monitoring and consultation of patients during COH in IVF-ET cycle.

• Journal of Microbiology and Biotechnology
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• 제24권7호
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• pp.969-978
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• 2014

The aprE2 gene with its prosequence from Bacillus subtilis CH3-5 was overexpressed in Escherichia coli BL21(DE3) by using plasmid pET26b(+). After IPTG induction, active and mature AprE2 was produced when cells were grown at $20^{\circ}C$, whereas inactive and insoluble enzyme was produced in a large amount when cells were grown at $37^{\circ}C$. The insoluble fraction was resuspended with 6 M guanidine-HCl and dialyzed against 2 M Tris-HCl (pH 7.0) or 0.5 M sodium acetate (pH 7.0) buffer. Then active AprE2 was regenerated and purified by a Ni-NTA column. Purified AprE2 from the soluble fraction had a specific activity of $1,069.4{\pm}42.4U/mg$ protein, higher than that from the renatured insoluble fraction. However, more active AprE2 was obtained by renaturation of the insoluble fraction. AprE2 was most stable at pH 7 and $40^{\circ}C$, respectively. The fibrinolytic activity of AprE2 was inhibited by PMSF, but not by EDTA and metal ions. AprE2 degraded $A{\alpha}$ and $B{\beta}$ chains of fibrinogen quickly, but not the ${\gamma}$-chain. AprE2 exhibited the highest specificity for N-succinyl-Ala-Ala-Pro-Phe-pNA. The $K_m$ and $k_{cat}/K_m$ of AprE2 was 0.56 mM and $3.10{\times}10^4S^{-1}M^{-1}$, respectively.

• BMB Reports
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• 제40권6호
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• pp.1002-1008
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• 2007

PreS domain of Hepatitis B virus (HBV) surface antigen is a good candidate for an effective vaccine as it activates both B and T cells besides binding to hepatocytes. This report deals with overexpression and purification of adr subtype of surface antigen that is more prevalent in Pakistan. PreS region, comprising 119 aa preS1 region plus a 55 aa preS2 region plus 11 aa from the N-terminal S region, was inserted in pET21a+ vector, cloned in E. coli $DH5\alpha$ cells and expressed in E. coli BL21 codon+ cells. The conditions for over expression were optimized using different concentrations of IPTG (0.01-5 mM), and incubating the cells at different temperatures (23-$41^{\circ}C$) for different durations (0-6 h). The cells were grown under the given optimized conditions (0.5 mM IPTG concentration at $37^{\circ}C$ for 4 h), lysed by sonication and the protein was purified by ion exchange chromatography. On the average, 24.5 mg of recombinant protein was purified per liter of culture. The purified protein was later lyophilized and stored at $-80^{\circ}C$.

• Journal of Preventive Medicine and Public Health
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• 제6권1호
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• pp.133-160
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• 1973

1. Introduction Community medicine with the concept of comprehensive medical care and an ideal medical care delivery system not only for an individual or family but for the whole community has emerged. In April 1970, the Presbyterian Medical Center started a hospital based community health service project in order to improve the health of the people in rural areas. Prior to commencing a comprehensive medical care system, a family survey was needed. The major objective of this survey was to obtain information concerning the people and their environment so as to be able to plan and implement a comprehensive medical care program in Soyang-Myun. 2. Survey Method An interview using a family record form was carried out for each household. This family record form was designed to get information about demography, family planning, environmental sanitation and vital statistics. Prior to beginning, the members of the survey team were trained in interviewing techniques for three days. The team consisted of a public health nurse, four nurse-aides, a sanitarian and four health extension workers who are working in our project, The survey was carried out during the period November 1971 to March 1972. 3. Project area 1) Population of Soyang-Myun was 11,668; male, 5,962 and female, 5,706. Sex ratio: 104.5. 2) Households : 1,858 3) Family size: The average household consisted of 6.3 persons. 4) Educational level of householder a. Illiterate 13% b. No schooling but able to read 10% c. Preschool children 19% d. Primary school 47% e. Middle school 7% f. High school 3% g. College or University 1% 5) Occupational distribution of householders a. Farmer 67% b. Laborer 13% c. Office worker 4% d. Merchant 4% e. Industrial worker 2% f. Unemployed 8% g. Miscellaneous 2% 6) Religious affiliation a. No religion 74% b. Buddhist 12% c. Protestant 10% d. Catholic 4% 4. Survey results Living Environment : a. Home ownership 95% b. Kinds of roofing Straw-thatched house 84% Tile-roofed house 10% Slate-roofed house 5% Other 1% c. Floor space Less than 6 pyong 10% 6-10 pyong 53% 11-15 pyong 24% 16-20 pyong 9% More than 20 pyong 4% d. Radio ownership 80% Environmental Sanitation : a. the source of drinking water public well 49% private well 30% drainage water 9% steam water 8% well pump 3% water distribution system 1% b. Distance between well and toilet more than 16meters 38% 6-10 meter 31% 11-15 meters 14% Less than 6 meters 17% c. The status of well management Bad 72% Fair 26% Good 2% d. General sanitary state of house Bad 37% Fair 51% Good 12% e. House drainage system had no house drainage. 77% Family Planning : a. 24% of the people have used contraceptives, but 12% ceased to use them. 76% have never used contraceptives. b. used methods 1oop 68% oral pill 16% vasectomy 4% condom 1% tubal ligation 1% two or more methods 10% Maternal Health : a. The number of conceptions of housewives under 50 years of age. 11 times 26% 6 times 11% 5 times 11% 4 times 9% b. The place of delivery own house 88% hospital 1% others 11% Treatment of general sickness : a. The place of treatment Soyang Health Center 31% Hospital (private or otherwise) 26% Pharmacy 14% Herb medicine 5% Private care 5% No treatment 12% Miscellaneous 7% b. Usual causes of diseases Unknown 46% Tuberculosis 29% Neuralgia 8% CVA 3% Bronchitis 3% Others 11%

• 한국식품위생안전성학회지
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• 제21권1호
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• pp.41-46
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• 2006

최근 생식제품에 대한 국민 선호도가 높아지고 판매량이 증가됨에 따라 안전하고 위생적인 생식의 유통을 유도하고, 위생 강화를 위한 미생물 규격 제정의 기초연구를 위하여 시판 생식 및 원재료에 대한 식중독균 분포실태를 조사하였다. 2002년부터 2004년까지 서울, 인천, 부산 등 전국 대도시를 중심으로 대형마트 및 통신판매에서 시판 중인 생식제품 191건을 구입하여 주요 식중 독균인 E. coli, Salmonella, Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, Listeria monocytogenes, Campylobactey jejuni, Yersinia enterocolitica, E. coli O157:H7, Vibrio parahaemolyticus를 검사하였다. 그 결과 B. cereus는 29건(15.2%), C. perfringens는 21건(11.0%), E. coli는 1건(0.5%)에서 검출되었으나 그 외 식중독균은 검출되지 않았다. 정량적 평가 결과 일반세균수는 $1.2X10^3cfu/g{\sim}1.6X10^9cfu/g$, B. cereus는 100 cfu/g 이하${\sim}11X10^3cfu/g$, C. perfringens는 100 cfu/g 이하${\sim}2.2x10^3cfu/g$의 분포를 보였다. 생식의 미생물 오염원을 파악하기 위하여 9개 생식제조공장에서 사용되고 있는 현미, 당근, 율무, 옥수수 등 53건의 원재료에 대한 미생물 분포도를 조사하였다. 그 결과 일반세균수는 $1.0X10^3cfu/g{\sim}1.5X10^8cfu/g$의 분포를 보였고, B. cereus는 13건(24.5%)에서 검출되었는데 11건(84.6%)이 100 cfu/g 이하이었다. C. perfringens는 2건(3.8%)에서 검출되었는데 모두 100 cfu/g 이하의 낮은 균수를 보였다. 생식 완제품과 같이 E. coli, Salmonella, S. aureus 등 8종의 식중독균은 검출되지 않았다. 본 실험 결과 유통 생식은 일반적으로 식중독균에 의한 위해 가능성이 적은 것으로 추정되었다. 그러나 제조과정 및 유통과정 중 부적절한 보관 및 유통에서 기인될 수 있는 오염균의 증식이나 교차오염 예방을 위한 철저한 관리는 필요한 것으로 판단되었다.