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In vitro cytotoxicity of four kinds orthodontic band cements (수종 치과 교정용 밴드 시멘트의 세포독성에 관한 실험적 연구)

  • Lee, Won-Chul;Park, Soo-Byung
    • The korean journal of orthodontics
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    • v.34 no.4 s.105
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    • pp.351-362
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    • 2004
  • Orthodontic band cements are widely used in the fields of orthodontics, but they are commonly known as cytotoxic material. Within an oral cavity several ions and components are released from orthodontic band cements, thus causing inflammation or injury to the Periodontal tissue. Therefore, it is very important to estimate the biocompatibility of orthodontic band cements. The purpose of this study was to assess the cytotoxic effect of orthodontic band cements to HGF cells. A zinc phosphate cement, a glass ionomer, a resin modified glass ionomer, and compomer were used to evaluate three cytotoxicity assays: cell proliferation assay, MTT assay, and agar ovelay assay The results were as follows: 1. In the cell proliferation assay, Gl>ZPC, RMGI, RMGI24, GI24>compomer24, ZPC24, compomer>metal ring lined up in order of cytotoxicity 2. In the MTT assay, GI>ZPC, RMGI>GI24>ZPC24, compomer, metal ring, RMGI24, compomer24 lined up in order of cytotoxicity. 3. In the agar overlay test, GI>GI24, ZPC, ZPC24, RMGI>RMGI24, compomer, compomer24, metal ring lined up in order of cytotoxicity.

Changes in Metals (Pb, Sn and Fe), Vitamin C Contents, Color and pH of Canned Pineapple Juice and Slice during Open Storage (캔 파인애플 쥬스 및 슬라이스의 개봉 후 저장조건에 따른 금속(Pb, Sn and Fe), Vitamin C. 색도 및 pH 변화)

  • 이숙경;손종성
    • Journal of Food Hygiene and Safety
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    • v.14 no.1
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    • pp.115-121
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    • 1999
  • The effect of storage temperature and time on the contents of metal (Pb, Sn and Fe), vitamin C, color and pH was studied for canned pineapple juice (PJ) and pineapple slice (PS) which were stored for 120 hours at 5 and 2$0^{\circ}C$ and analyzed at 24 hours intervals. The results are as follows; 1. The metal contents of PJ and PS were in the rank of 24<48<72<96<120 hours by storage time at 5 and 2$0^{\circ}C$. These contents were increased to 44.1%/24 hrs of Ph, 18.0%/24 hrs of Sn, 34.6%/24 hrs of Fe but decreased to 6.0%/24 hrs of vitamin C in PJ and PS during 120 hrs. Storage times were correlation to contents of metal and pH but was not correlation to vitamin C contents. These were increased to 37.7%/24 hrs of Pb, 18.8%/24 hrs of Sn, 34.6%/24 hrs of Fe, but decreased to 6.0%/24 hrs of vitamin C. 2. These were increased to 10.6% of Pb, 3.7% of Sn, 11.3% of Fe in PJ and to 33.7% of Pb, 4.8% of Sn, 37.6% of Fe in PS at 2$0^{\circ}C$ than 5$^{\circ}C$ but vitamin C contents were decreased to 8.2% in PJ and 2.7% in PS at 2$0^{\circ}C$ than 5$^{\circ}C$. This fact suggests that more attention be paid in handling canned PJ and PS after opening in order to avoid the decreasing vitamin C and the hazard from Pb, Sn, Fe. 3. Changing factors in Pb, Sn, Fe and vatiamin C content were in the rank of storage temperature$0^{\circ}C$.

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Adherence Rate of Listeria monocytogenes Contaminated from Pork Meat During Pork Meat Processing (포장돈육 가공공정 중 Listeria monocytogenes에 오염된 돈육으로부터 오염 부착율)

  • Kim, Seong-Jo;Bahk, Gyung-Jin;Oh, Deog-Hwan
    • Journal of Food Hygiene and Safety
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    • v.22 no.4
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    • pp.332-337
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    • 2007
  • This study was done to determine the adherence rate of Listeria monocytogenes(LM) from pork meat to pork meat, conveyer belt and stainless steel, and cutting board at $10^{\circ}C$ for given times (2, 4, 24 hours). The adherence rates of ATCC and wild type LM from pork meat to red meat or fat meat after 2 hour contact time were approximately 65-79%, but 100% adherence rates of both LM after 6 hours were observed. The adherence rate of ATCC LM from pork meat to conveyer belt and stainless steel after 24 hours was 0.15%, while the adherence rate of wild type LM was rapidly increased from 4.34 after 2 hours to 28.6% after 24 hours. Also, The adherence rate of ATCC LM from pork meat to stainless steel after 24 hours ranged from 2.63% after 2 hours to 0.39% after 24 hours, while the adherence rate of wild type LM ranged from 0% after 2 hours to 24.4% after 24 hours. In the meantime, no adherence rate of both LM from pork meat to cutting board was observed. This results suggest that the adherence rate of LM from pork meat to pork meat or food contact surfaces was significantly affected by contact time and wild type LM showed much higher adherence rate to pork meat or food contact surfaces than ATCC LM.

The Usefulness of Spot Urine Protein/Creatinine Ratio in Evaluating Proteinuria in Children and the Correlation between 24-hour Urinary Protein Amount and Spot Urine Protein/Creatinine Ratio (소아 단백뇨 검사에 있어서 단회뇨 단백/크레아티닌 비의 유용성 및 일일 요단백량과의 연관성)

  • Hong, Seon Young;Kim, Ji Young;Chung, Woo Yeong
    • Clinical and Experimental Pediatrics
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    • v.46 no.2
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    • pp.173-177
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    • 2003
  • Purpose : Recently, different results about factors affecting accurate quantitation of 24-hr urinary protein(24UP) amount using spot urine protein/creatinine ratio(PCR) have been reported. The current study was designed to evaluate correlation between 24UP amounts and PCR in children, and the effect of 24UP amounts, age, sex, and glomerular filtration rate(GFR) on this correlation. Methods : Among 94 patients who visited the department of pediatrics in Busan Paik Hospital from March 2002 to August 2002, 68 patients whose urinary creatinine excretion was ${\geq}15mg/kg/day$ were included in this study. All the patients were divided into I, II/A, B group(I : 24UP<500 mg/day, II : $24UP{\geq}500mg/day$, A : <10 years of age, B : ${\geq}10years$ of age). Pearson correlation analysis was performed between 24UP and PCR to evaluate the relationship. We defined fractional difference between 24UP and PCR, and then performed multiple regression analysis with 24UP amount, age, GFR and fractional difference. Results : There was a strong positive linear correlation between 24UP and PCR(R=0.936, P<0.0001) in all patients, and the correlation was also good in each group. Using PCR cutoff values of 0.5, the PCR provided high sensitivity, specificity, positive and negative predictive value in predicting 24UP amount ${\geq}500mg$. The factors affecting accurate quantitation of proteinuria using spot urine PCR was age, not 24UP amount, GFR or sex. Conclusion : Spot urine PCR is a useful test but has limitations in predicting 24UP amount. Therefore, it should be used only as screening method. Age-adjusted PCR cutoff values may be necessary to predict 24UP amount in children with proteinuria.

Solvent Extraction of Cu(II) by 2,4-Heptadione in Chloroform (2, 4-Heptadione에 의한 Cu(II)의 용매추출특성)

  • Shin, Jeong-Ho;Jeong, Kap-Seop;Lee, Sang-Hoon;Park, Sang-Wook;Park, Dae-Weon
    • Applied Chemistry for Engineering
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    • v.4 no.4
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    • pp.672-682
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    • 1993
  • 2, 4-Heptadione(abbreviated to 24HTD) was synthesized from methylpropyl ketone and ethyl acetate with sodium amide, and the equilibrium and the kinetic characteristics of copper extraction by 24HTD-chloroform were investigated. Equilibrium constants such as the dissociation constant and the distribution coefficient of 24HTD and the stability constant of the 24HTD-Cu chelate were evaluated from the spectrophotometry, and the overall equilibrium constant of the extraction was also determined. The extracted species of the 24HTD-Cu chelate was found to be $CuR_2$ and the initial rate of the extraction of coupper by 24HTD in chloroform was expressed by $R_0=k[\bar{HR}]([Cu^{2+}]/[H^+])^{0.5}$.

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Complete genome sequence of a novel bacteriophage SPG24 isolated from Cheonggukjang (청국장에서 분리된 신생 박테리오파지 SPG24의 전체 염기 서열)

  • Kim, Chaehyun;Lee, Gyu-Cheol;Kim, In Kyo;Kim, Seok Cheon;Lee, Oh Hyung;Lee, Chan Hee
    • Korean Journal of Microbiology
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    • v.53 no.2
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    • pp.144-145
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    • 2017
  • A Bacillus subtilis strain, designated G24, was isolated from grape during a study on the fermentation of Cheongkukjang, Korean traditional fast-fermented bean paste. Also, a newly isolated bacteriophage SPG24, which was found to inhibit the fermentation process, uses B. subtilis G24 as a host. The complete genome sequence of the bacteriophage SPG24 was 152,060 bp in length, with a G+C content of 42.2%. This sequence included 232 ORF; 58 forward ORFs and 174 reverse ORFs.

The Factors Affecting Accurate Quantitaion of Proteinuria Using Sp ot Urine Protein/Creatinine Ratio in Children (소아 단백뇨 검사에 있어서 단회뇨 단백 크레아티닌 비에 영향을 미치는 요인)

  • Jung, Ji-Mi;Kwon, Eun-Ji;Chung, Woo-Yeong
    • Childhood Kidney Diseases
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    • v.12 no.2
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    • pp.150-156
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    • 2008
  • Purpose : Many results have reported a correlation between the spot urine protein/creatinine ratio(P/C ratio) and 24-hour urinary protein(24UP) amount. This study was designed to evaluated correlation between 24UP amounts and P/C ratio in children and to find the factors that affect this correlation. Methods : 210 patients who visited the Department of Pediatrics in Busan Paik Hospital from september 2003 to december 2007 were included in this study. All the patients were divided into I, II, III/A, B, C group[I:24UP(mg/$m^2$/day)]<100, II: 100$\leq$24UP<1,000, III: 24UP$\geq$1,000, A: Cr excretion(mg/kg)<15, B: 15$\leq$Cr excretion<25, C: Cr excretion$\geq$25)]. Pearson correlation analysis was performed between 24UP and P/C ratio to evaluate the relationship. We defined fractional difference between 24UP and P/C ratio, and then performed multiple regression analysis. Results : There was a strong positive linear correlation between 24UP and P/C ratio in all patients, and the correlation was also good in each group. The factors affecting accurate quantitation of proteinuria using spot urine P/C ratio was creatinine excretion. Conclusion : Spot urine P/C ratio is a useful test to predict proteinuria roughly. Therefore, we expect that urine P/C ratio can be used as parameter instead of 24UP, if we set cutoff value of P/C ratio considered to creatinine excretion according to age and sex in large pediatric population.

Influence of Age at Weaning on Caecal Content Characteristics and Post-weaning Performance and Health of Rabbits

  • Taranto, S.;Di Meo, C.;Stanco, G.;Piccolo, G.;Gazaneo, M.P.;Nizza, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.10
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    • pp.1540-1544
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    • 2003
  • This study chiefly aimed to ascertain the effect of age at weaning on caecal content characteristics, post-weaning performance and health of rabbits. A secondary aim was to study the effect of dietary restriction on performance and health of rabbits. After delivery, 30 litters of 8 pups each, were housed in separate cages. Fifteen litters were weaned 24 days from birth (group W 24) while the other 15 were weaned at 28 days (group W28). After weaning, 53 rabbits of group W24 and 56 of group W28 were fed ad libitum (group LIB), while 53 rabbits of group W24 and 57 of group W28 were restricted (group RES). All the rabbits were kept in individual cages. Fourteen other rabbits of groups W24 (7 fed ad libitum and 7 restricted) and 7 of group W28 were sacrificed to obtain data regarding caecum weight and caecal content. Age at weaning affected neither the state of health nor the final live weight. In comparison with those of group W28, the rabbits weaned at 24 days had a lower daily gain until 35 days of age but gain was higher subsequently. From the 28th day, however, the feed intake and the feed conversion ratio of the group W24 rabbits were always better than those of W28. The restricted diet until 42 days resulted in a lower weight both at 42 days (1,078 vs. 1,164 g) and at 70 days (2,192 vs. 2,294 g). Indeed, from the 42nd day, despite receiving feed ad libitum the rabbits of the restricted group continued to ingest less feed (120.8 vs. 127.2 g) than those of the ad libitum group, and had a slightly lower daily gain (39.8 vs. 40.3 g). The measurements carried out on the caecum highlighted significant differences among the groups in caecal content (33.1 vs. 31.7 vs. 25.3 g respectively for groups W24LIB, W28 and W24RES), for total volatile fatty acids (53.2 vs. 50.6 vs. 44.5 mmol/l respectively for groups W24LIB, W28and W24RES) for ammonia (7.0 vs. 7.2vs 8.8 mmol/l respectively for groups W24LIB, W28 and W24RES), for propionate (5.7 vs. 6.3 vs. 8.1% respectively for groups W24LIB, W28 and W24RES) and for propionate/butyrate (0.33 vs. 0.36 vs. 0.49 respectively for groups W24LIB, W28 and W24RES).

Growth and Yield Responses of Soybean to Overhead Flooding Duration at Four Growth Stages (관수시간에 따른 콩의 생육 및 수량반응)

  • 박경열;이종형;조영철
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.1
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    • pp.92-97
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    • 1995
  • The objective of this study is to investigate the growth characters of overhead flooded soybean plants at four growth stage. Overhead flooding treatments were applied at the vegetative growth stage ($V_3,\;V_6$) and the reproductive stage ($R_2,\;R_4$) for 6.12.24 hrs, respectively. Yield and yield components were more decreased as the overhead flooding duration was longer and the growth stage was later. Yield was not reduced significantly in soybean plants flooded at $V_3$ stage regardless of flooding duration, and flooded 6 or 12 hrs at $V_6$ stage. When compared to the control, 27 to 36% of yield reduction was observed in soybean plants flooded for 24 hrs at $V_6$ stage, 6 or 12 hrs at $R_2$ stage, and 6 hrs at $R_4$ stage. And 43%, 53% and 66% of yield were reduced through the flooding treatment for 24 hrs at $R_2$ stage 12 hrs and 24 hrs at $R_4$ stage, respectively. So yield reduction was higher in overhead flooded soybean plants at the reproductive stage than that at the vegetative growth stage.

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Differentiation of Bone Metastases and Fractures using 24 hour/3 hour Radio-uptake Ratio in Bone Scintigraphy (골신티그라피의 3시간과 24시간 방사능 섭취비를 이용한 골 전이와 골절의 감별)

  • Han, Song-Yee;Chun, Kyung-Ah;Chung, Yong-Ahn;Kim, Sung-Hoon;Kim, Young-Joo;Chung, Soo-Kyo;Park, Seog-Hee
    • The Korean Journal of Nuclear Medicine
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    • v.33 no.6
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    • pp.512-518
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    • 1999
  • Purpose: We evaluated the usefulness of 24 hour/3 hour radio-uptake ratio, lesion to non-lesion ratio, in differentiating bony metastases from acute (<2 months) and healing (${\geq}2$ months) fractures. Materials and Methods: Sixty-three patients (age range: 26-81, 32 males, 31 females) having 90 lesions (30 bone metastases, 30 acute fractures, 30 healing fractures) were included. Bone scans were obtained 3 and 24 hours after administration of 740 MBq of $^{99m}Tc$-MDP. The ratio of radio-uptake in the lesion to normal area was measured as 24/3 hour radio-uptake ratio ([lesion/non-lesion RUR at 24 hour]/[lesion/non-lesion RUR at 3 hour], 24/3 RUR) and analyzed clinical significance in differentiating bone metastases from acute or healing fractures. Results: Mean 24/3 RUR were $1.22{\pm}0.18$ for bone metastases, $1.25{\pm}0.14$ for acute fractures, and $0.99{\pm}0.15$ for healing fractures. 24/3 RUR values of bone metastases and acute fractures were not significantly different. But 24/3 RUR values of bone metastases and healing fractures, and those of acute and healing fractures were found to be significantly different (p<0.001). When 24/3 RUR of 1.0 was considered as the cut off point separating metastases from fracture, a sensitivity of 100% (30/30) was obtained. The specificity was 0% (0/30) in separating metastases from acute fractures, and 47% (14/30) in separating metastases from healing fractures. When 24/3 RUR of 1.2 was considered as the cut off point, sensitivity of 53% (16/30) in the diagnosis of bone metastasis, and specificity of 37% (11/30) in separating metastases from acute fractures, and 100% (30/30) in separating metastases from healing fractures were obtained. Conclusion: 24/3 RUR is useful in differentiating bone metastases from healing fractures, but not in differentiating bone metastases from acute fractures. A 24/3 RUR of less than 1.0 suggests healing fractures. A 24/3 RUR of more than 1.2 suggests bone metastases or acute fractures.

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