• The Korean Journal of Physiology and Pharmacology
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• v.26 no.6
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• pp.457-468
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• 2022

It has been demonstrated that APPL1 (adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1) is involved in the regulation of several growth-related signaling pathways and thus closely associated with the development and progression of some cancers. Diallyl trisulfide (DAT), a garlic-derived bioactive compound, exerts selective cytotoxicity to various human cancer cells through interfering with pro-survival signaling pathways. However, whether and how DAT affects survival of human hepatocellular carcinoma (HCC) cells remain unclear. Herein, we tested the hypothesis of the involvement of APPL1 in DAT-induced cytotoxicity in HCC HepG2 cells. We found that Lys 63 (K63)-linked polyubiquitination of APPL1 was significantly decreased whereas phosphorylation of APPL1 at serine residues remained unchanged in DAT-treated HepG2 cells. Compared with wild-type APPL1, overexpression of APPL1 K63R mutant dramatically increased cell apoptosis and mitigated cell survival, along with a reduction of phosphorylation of STAT3, Akt, and Erk1/2. In addition, DAT administration markedly reduced protein levels of intracellular TNF receptor-associated factor 6 (TRAF6). Genetic inhibition of TRAF6 decreased K63-linked polyubiquitination of APPL1. Moreover, the cytotoxicity impacts of DAT on HepG2 cells were greatly attenuated by overexpression of wild-type APPL1. Taken together, these results suggest that APPL1 polyubiquitination probably mediates the inhibitory effects of DAT on survival of HepG2 cells by modulating STAT3, Akt, and Erk1/2 pathways.

• Proceedings of the Materials Research Society of Korea Conference
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• 1998.08a
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• pp.87.4-87
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• 1998

• Journal of Sericultural and Entomological Science
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• v.39 no.1
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• pp.93-94
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• 1997

• Molecules and Cells
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• v.40 no.1
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• pp.73-81
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• 2017

The ${\gamma}$-secretase complex represents an evolutionarily conserved family of transmembrane aspartyl proteases that cleave numerous type-I membrane proteins, including the ${\beta}$-amyloid precursor protein (APP) and the receptor Notch. All known rare mutations in APP and the ${\gamma}$-secretase catalytic component, presenilin, which lead to increased amyloid ${\beta}$-peptide production, are responsible for early-onset familial Alzheimer's disease. ${\beta}$-amyloid protein precursor-like (APPL) is the Drosophila ortholog of human APP. Here, we created Notch- and APPL-based Drosophila reporter systems for in vivo monitoring of ${\gamma}$-secretase activity. Ectopic expression of the Notch- and APPL-based chimeric reporters in wings results in vein truncation phenotypes. Reporter-mediated vein truncation phenotypes are enhanced by the Notch gain-of-function allele and suppressed by RNAi-mediated knockdown of presenilin. Furthermore, we find that apoptosis partly contributes to the vein truncation phenotypes of the APPL-based reporter, but not to the vein truncation phenotypes of the Notch-based reporter. Taken together, these results suggest that both in vivo reporter systems provide a powerful genetic tool to identify genes that modulate ${\gamma}$-secretase activity and/or APPL metabolism.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.5
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• pp.650-659
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• 2013

To investigate the effects of facial blood flow rates(FBFR) and facial skin temperature(FST) generated by Gagam-Jawoonaek(GJ) application(appl.) after Miso Facial Rejuvenation Acupuncture(MFRA). Ten people in their twenties to fifties with no skin diseases were recruited. We randomly divided subjects two groups(A, B) and set the GJ appl. site(group A - right side, group B - left side). MFRA was performed on both sides of their face. Immediately after acupuncture treatment(AT treat.), GJ was applied only half of the face. We measured their FBFR using Laser Doppler Perfusion Imaging(LDPI) and FST using Digital Infrared Thermal Imaging(DITI) at pre-AT treat., immediately after AT treat., twenty and sixty minutes after GJ appl.. We analyzed data using Mann-Whitney test and Wilcoxon test(p < 0.05). After MFRA treat., FBFR on both sides increased. Twenty minutes after JW appl., the changes of FBFR on GJ appl. side($122.9{\pm}43.1PU$) were bigger than GJ non-appl. side($80.9{\pm}38.4PU$), a statistically significant decrease. Sixty minutes after application, FBFR on both sides were recovered almost at the same level as that of pre-AT treat. After MFRA treat., FST on both sides increased. Twenty minutes after GJ appl., the changes of FST on GJ appl. side($1.1{\pm}0.6^{\circ}C$) were comparable to that of GJ non-appl. side($1.2{\pm}0.5^{\circ}C$). Sixty minutes after application, FST on both sides were recovered almost at the same level as that of pre-AT treat.. Gagam-Jawoonaek could decrease facial blood flow rates.

• Journal of Korean Ophthalmic Optics Society
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• v.8 no.1
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• pp.23-28
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• 2003

We report a switchable RGB color filters for Lab on a chip. Ink color filter devices are fabricated by PDMS patterning process. The optical transmission in the ultra-violet and visible wavelengths of color filters have been investigated. The ink color filters are characterised by the slope and shape of the spectrum. The transmission of red/yellow filter consists of curves with a sharp cut-off spectrum at 600 and 500 nm. The blue output of the ink filter has 80% peak transmission and weak red-leak, while the green ink filter has 48% peak transmission and red-leak.

• Communications of the Korean Mathematical Society
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• v.24 no.1
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• pp.29-40
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• 2009

Motivated by [Linear Algebra and its Appl. 420(2007), 218-227] and [Linear Algebra and its Appl. 425(2007), 171-183], we, in this paper, study the solvability of periodic boundary value problems of higher order nonlinear functional difference equations with p-Laplacian. Sufficient conditions for the existence of at least one solution of this problem are established.

• Journal of applied mathematics & informatics
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• v.28 no.1_2
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• pp.397-409
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• 2010

We prove the existence of common fixed points for multivalued maps satisfying a contractive condition of an integral type. Our results are extent ions of results of Feng and Liu[Y. Feng, S. Liu, Fixed point theorems for multi-valued contractive mappings and multi-valued Caristi type mappings, J. Math. Anal. Appl. 317(2006), 103-112] and also, extent ions of results of Daffer and Kaneko[P. Z. Daffer, H. Kaneko, Fixed points of generalized contractive multi-valued map pings, J. Math. Anal. Appl. 192(1995), 655-666]. A main result in Feng and Liu[Y. Feng, S. Liu, Fixed point theorems for multi-valued contractive mappings and multi-valued Caristi type mappings, J. Math. Anal. Appl. 317(2006), 103-112] is proved under necessary additional conditions.

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.1
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• pp.94-101
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• 2014

We studied to investigate the erythema reduction effects generated by Gagam-Jawoonaek(GJ) application(appl.) after UV exposure. Twenty women in their twenties to fifties with no skin diseases were recruited. We exposed UV as a 6 subsites on the left upper arm of subjects using multi-port solar simulator. After setting Gagam-Jawoonaek(GJ) application(appl.) subsites and non-appl. subsites, we measured erythema degrees($a^*$ values) of the subsites using spectrophotometer. We measured $a^*$ values four times(before UV exposure, before application of GJ, twenty-four and forty-eight hours after first application of GJ). We analyzed data using student's t-test. After UV exposure, $a^*$ values on the left upper arm increased. Twenty-four hours after first GJ treat., the changes of $a^*$ value on GJ treat. subsites($1.22{\pm}0.13AU$) were bigger than GJ non-treat. subsites($1.04{\pm}0.12AU$), but there was no statistically significance. Forty-eight hours after first GJ treat., the changes of $a^*$ value on GJ treat. subsites($1.95{\pm}0.11AU$) were bigger than GJ non-treat. subsites($1.58{\pm}0.13AU$), a statistically significance. Gagam-Jawoonaek could decrease erythema by UV exposure.

• Journal of applied mathematics & informatics
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• v.10 no.1_2
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• pp.101-109
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• 2002

We extend two inequalities involving Hadamard Products of Positive definite Hermitian matrices to positive semi-definite Hermitian matrices. Simultaneously, we also show the sufficient conditions for equalities to hold. Moreover, some other matrix inequalities are also obtained. Our results and methods we different from those which are obtained by S. Liu in [J. Math. Anal. Appl. 243:458-463(2000)] and B.-Y Wang et al in [Lin. Alg. Appl. 302-303: 163-172(1999)] .