• 한국응용과학기술학회지
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• 제26권2호
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• pp.110-116
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• 2009

In an attempt to find natural sources of antioxidants and whitening agents, Comparisons of the antioxidative and tyrosinase inhibitory activities of various ethanol extracts of Astragali Radix, Atractylodis Rhizoma Alba and Acanthopanacis Cortex were carried out. Comparison of the four ethanol extracts revealed that, Astragali Radix had the highest electron-donating ability(72.5%) and the highest SOD-like ability(26.1%). The xanthine oxidase experiment exhibited a hindrance effect of 88.5% in Atractylodis Rhizoma Alba, 81.1% in Acanthopanacis Cortex, 75.8% in Astragali Radix. A tyrosinase inhibitory activity assay was conducted to evaluate the whitening effects of the extracts, The tyrosinase inhibitory activity was 42.1% in the Acanthopanacis Cortex, 37.2% in the Atractylodis Rhizoma Alba, 6.0% in the Astragali Radix. Based on these results, we suggest that the ethanol extracts of Astragali Radix, Atractylodis Rhizoma Alba and Acanthopanacis Cortex can be used as food and cosmetic ingredients.

• 동의생리병리학회지
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• 제20권3호
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• pp.657-662
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• 2006

Immunological activities of the combined-administration of Acanthopanacis Cortex and Lycii Cortex Radicis were examined in BALB/C mice. The 40% ethyl alcohol extract of Acanthopanacis Corter (AE) or the 40% ethyl alcohol extract of Acanthopanacis Coriex and Lycii Cortex Radicis (ALE) were administered p.o. once a day for 7 days, respectively. AE did not affect the viability of thymocytes, but ALE decreased the viability of thymocytes. ALE enhanced the viability of splenocytes increased by AE. Also, AE enhanced the population of cytotoxic T cell in thymocytes, and ALE enhanced the population of helper T cell compared with AE. Furthermore, AE increased the population of $Thy1^+$ cells in splenocytes, and increased the population of splenic $CD4^+$ cells. In addition, ALE enhanced the phagocytic activity which was decreased by AE ALE decreased the production of nitric oxide increased by AE in peritoneal macrophages. These results suggest that ALE enhance an immune-regulative action of AE.

• 혜화의학회지
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• 제10권2호
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• pp.179-188
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• 2002

This experimental study was carried out to evaluate the effects of Acanthopanacis cortex on Cytokine-inducing and and immune response in Mice. In order to investigate the effect of Acanthopanacis cortex, the following was performed; Cytotoxicity, in vitro, the fraction of $CD4^+$, $CD8^+$, $B220^+$ in splenic cell, gene expression of IL-12(p35), IL-12(p40), IFN-${\gamma}$, and splenic cell proliferation by Acanthopanacis cortex. Analysis of cytokine gene expression was carried out by RT-PCR amplification. Amplified PCR products were electrophoresed on 1.2% agarose gel, and the analysis (Ht) was used to 1D-density program. The results were obtained as follows. Acanthpanacis cortex showed didn't have cell toxicity under $12{\mu}g/m{\ell}$ group on mouse lung fibroblast cells. In an in vitro model using mouse peripheral blood mononuclear cells (PBMCs), extract of Acanthpanacis cortex induced multiple cytokine, including interleukin-12 (p35), interleukin-12 (p40), interferon-gamma (IFN-${\gamma}$). The extract also enhanced the percentages of the $CD4^+$, and $CD8^+$ in the untreated control were $22.1{\pm}3.3$ to $38.4{\pm}2.1$, and $5.0{\pm}0.4$ to $10.7{\pm}0.3%$, respectively. From above findings, it is suggested that Acanthopanacis cortex is able to anti-cancer and activate immune response system.

• Journal of Acupuncture Research
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• 제31권1호
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• pp.131-137
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• 2014

Objectives : This study is to investigate the effects of Acanthopanacis Cortex hot aqueous extract on nitric oxide(NO), prostaglandin E2(PGE2) production and DPPH(1,1-diphenyl-2-picryl hydrazyl) radical scavenging activity in macrophages. Methods : Acanthopanacis Cortex(200 g) was heated at $100^{\circ}C$ with distilled water(2 L) for 4hrs. The extract was filtered and concentrated to 100 ml using a rotary evaporator and was frozen at $-80^{\circ}C$, then was freeze-dried. The RAW 264.7 macrophages were subcultured. In order to evaluate cytotoxicity, MTT assay was performed. Experimental groups were divided into five(control, AC 25, 50, 100 and 200 ${\mu}g/ml$) and we measured cytotoxicity. The concentrations of NO were preprocessed by Griess assay. The RAW 264.7 macrophages was pretreated by 10 ${\mu}g/ml$ LPS and experimental groups were divided into five and we measured NO production. The concentrations of $PGE_2$ were measured by enzyme immunoassay. The RAW 264.7 macrophages was pretreated by 10 ${\mu}g/ml$ LPS. Experimental groups were divided into five and we measured $PGE_2$ production. Antioxidant activity was measured by the DPPH method. experimental groups were divided into four(AC 25, 50, 100 and 200 ${\mu}g/ml$) and we measured DPPH radical scavenging activity. Results : 1. Viability of RAW 264.7 macrophages did not significantly decrease in 25, 50 and 100 ${\mu}g/ml$ Acanthopanacis Cortex hot aqueous extract compared to control group. 2. NO production in LPS-stimulated RAW 264.7 macrophages significantly inhibited in 100, 200 ${\mu}g/ml$ Acanthopanacis Cortex hot aqueous extract compared to control group. 3. $PGE_2$ production in LPS-stimulated RAW 264.7 macrophages significantly inhibited in 100, 200 ${\mu}g/ml$ Acanthopanacis Cortex hot aqueous extract compared to control group. 4. DPPH radical scavenging capability of Acanthopanacis Cortex hot aqueous extract in RAW 264.7 macrophages had the high level in 100, 200 ${\mu}g/ml$. Conclusion : According to the results, Acanthopanacis Cortexx hot aqueous extract has ability to suppress NO, $PGE_2$ production and improve DPPH free radical scavenging activity. So Acanthopanacis Cortex hot aqueous extract may have an anti-inflammation effect and antioxidant activity.

• 동아시아식생활학회지
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• 제20권1호
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• pp.37-45
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• 2010

오가피 추출물이 고지방 식이를 급여한 흰쥐의 체내 지질대사, 심혈관계질환지수 항산화능 및 항산화 효소계에 어떤 영향을 미치는지 알아보고자 하였다. 연구 결과를 요약하면 아래와 같다. 1. 식이 섭취량은 고지방 식이군이 정상 식이군에 비해 유의적 (p<0.05)으로 감소하였으며, 식이 효율은 유의적 (p<0.05)으로 높았다. 최종 체중과 체중 증가량은 고지방 식이군과 정상 식이군 간에 유의적인 차이가 없었다. 2. 혈장의 HDL-콜레스테롤은 고지방 식이군에서 오가피 추출물의 첨가로 증가하였으며 LDL-콜레스테롤은 감소하였다(p<0.05). 중성지방은 고지방 식이군이 정상 식이군에 비해 증가하였으며, 오가피 추출물 섭취로 감소하였다(p<0.05). 간의 총 지질은 군 간에 차이가 없었고, 총콜레스테롤은 정상 식이군에 비해 고지방 식이군에서 유의하게 증가하였으나(p<0.05) 오가피 추출물의 첨가로 유의하게 감소하였으며(p<0.05), 중성지방 농도는 정상 식이군에 비하여 고지방 식이군에서 유의적으로 증가하였다(p<0.05). 3. 심혈관위험지수와 동맥경화지수는 고지방 식이군에 비하여 고지방 식이 오가피 추출물군에서 유의적으로 감소하였다(p<0.05). 4. 혈장과 간의 지질 과산화물 농도는 정상 식이군에서 오가피 추출물 섭취로 유의하게 감소하였다(p<0.05). 혈장의 TAS 값은 고지방 섭취로 감소되었으나, 오가피 추출물 섭취로 유의하게(p<0.05) 증가되었다. 5. 간의 SOD, catalase 및 GST 활성도는 정상 식이군과 고지방 식이군에서 오가피 추출물 첨가군이 오가피 추출물 무첨가군에 비해 유의적으로 증가하였고(p<0.05), glutathione peroxidase 활성도와 glutathione 함량은 고지방 식이군에서 오가피 추출물 첨가로 유의하게(p<0.05) 증가하였다. 이상의 결과에서 고지방 식이를 공급하였을 때 오가피 추출물 섭취는 혈장의 HDL-콜레스테롤 농도는 높이고, 혈장과 간의 총콜레스테롤과 중성지방의 농도는 낮추어 심혈관 위험지수와 동맥경화지수를 효과적으로 감소시키므로 지질 개선 및 고지혈증에 대한 개선 효과가 있을 것으로 보인다. 또한 항산화 효소 활성을 증가시켜 혈장과 간의 지질 과산화물 생성을 억제시키므로 항산화제로서 작용할 수 있음을 시사해 주고 있다. 그러나 지질 개선 및 천연 항산화제로서 작용할 수 있는 오가피의 용량에 대해서는 좀 더 많은 연구가 필요한 것으로 사료된다.

• 한국식품영양과학회지
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• 제21권1호
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• pp.9-16
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• 1992

This study was carried out to evaluate the effect of Acanthopanacis Cortex boiling extract solutions on the fat accumulation in the obese rats induced by the oral high fat administration for six weeks. Total cholesterol, neutral fat and adipose acid of ACR groups were lower than the control group. During the feeding experiment, LDL and VLDL were increased while HDL was decreased in all groups. Insulin and cortisol were higher than the control group, due to the fat accumulation. Based on the above results, it was shown that it is possible to improve fat accumulation induced by high fat dietary through using the oral administration of Acanthopanacis Cortex boiling extract solutions.

• 대한한의학회지
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• 제23권4호
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• pp.98-104
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• 2002

Objectives: Calpain, a calcium-dependent cysteine proteinase, may be one of the proteolytic enzymes that mediate cartilage degradation associated with rheumatoid arthritis. The object of this study is to ascertain immunohistochemically whether calpain is present in the inflamed joints of collagen-induced arthritis of rats, and examine the effect of Cortex Acanthopanacis Senticosi on the expression of calpain. Methods: Male Lewis rats, around 200g of body weight, were immunized with bovine type II collagen. After 3 weeks from first immunization, rats were divided into arthritic control (n=6) group and Cortex Acanthopanacis Senticosi-treated (n=6) group. Non-immunized rats served as the normal (n=6) group. All animals were sacrificed at 15 days post-treatment and tibiotarsal joints were removed. Calpain immunohistochemistry was performed on the midsagittal section of the tibiotarsal joint. Results: All animals of the control and treated groups showed ankylosing osteoarthritis. However, the animals of the treated group showed alleviation in the fibrous ankylosis, destruction of articular cartilage and destruction of subchondral bony tissue compared with the animals of the control group. Calpain was expressed in the chondrocyte lacunae of growing articular cartilage, in the skeletal muscle fibers, in the peripheral nerves, and in the vessel walls around the joints of all groups. In the control and treated groups, calpain was also expressed in proliferating synovial epithelia, subsynovial stroma cells, surface of articular cartilage, and fibrous pannus around destructive subchondral bony tissue. However, the expression density of calpain in the treated group was diminished compared with the control group, especially in surface of articular cartilage and fibrous pannus. Conclusions: These observations indicated that calpain plays an important role in the destruction of cartilage and bone in collagen-induced arthritis of rats, and also indicated that Cortex Acanthopanacis Senticosi inhibits the development of arthritis by decreasing the expression of calpain.

• 한국식품영양과학회지
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• 제37권4호
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• pp.521-527
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• 2008

오가피 추출액을 첨가하여 약주를 제조하고 발효 및 품질 특성을 조사하였다. 오가피 추출물 첨가 발효주는 대조구와 전체적으로 비슷한 발효 특성을 보였다. pH는 모든 처리구에서 발효 3일 만에 급격히 떨어졌고 발효 6일 이후로는 4.0 내외로 변화가 없었으며, 산도는 오가피 추출물 첨가구와 대조구 차이가 거의 없었다. 당도와 환원당은 모든 시험구에서 발효초기에 감소하다가 후기에는 완만하게 감소하였다. 발효주의 알코올 함량은 발효초기에는 대조구가 오가피 추출물 첨가구보다 높았으나 발효 6일째 이후로는 오가피 추출물 첨가구의 함량이 높았으며, 발효 종료일까지 점차적으로 증가하는 경향을 보였다. pH, 산도, 환원당 및 알코올 함량이 발효 $0{\sim}3$일 사이에 급격히 변화하는 것으로 나타나 담금 후 $0{\sim}3$일에 발효가 가장 왕성하게 일어나는 것으로 사료되었다. 유기산으로는 acetic acid, lactic acid, oxalic acid, malic acid 및 succinic acid가 검출되었으며, 전체 유기산 함량은 acetic acid의 함량이 가장 많았다. 오가피의 지표성분으로 알려져 있는 eleutheroside E와 chlorogenic acid는 2단 담금 후에는 두 유효성분의 함량 변화가 거의 없이 안정된 상태를 유지하였으며, 발효 종료일에는 발효주 중의 eleutheroside E 함량은 $7.61{\pm}0.39{\mu}g/mL$이었고, chlorogenic acid 함량은 $3.63{\pm}0.18{\mu}g/mL$이었다. Fusel oil 함량은 두 시험구에서 n-propyl alcohol, isobutyl alcohol 및 isoamyl alcohol 함량이 $0.08{\pm}0.001{\sim}0.86{\pm}0.03mg%$로 적은 양이 검출되었다. 관능검사 결과 오가피 추출물 첨가구는 전체적으로 모든 항목에서 대조구와 유사한 관능평점을 나타내었다.

• 한국식품영양과학회지
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• 제33권9호
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• pp.1457-1462
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• 2004

Wistar계 흰쥐에게 두릅, 오가피 및 느릅의 열수추출물을 급여하여 7주간 급여하였으며 희생 2주전에 STZ으로 당뇨를 유발하여 혈당이 300 ㎎/dL 이상인 흰쥐를 대상으로 혈장 중의 포도당 농도 및 바이오마커에 미치는 영향을 구명하였다. 혈장의 포도당 함량은 두릅나무과 식물의 열수추출물 급여시 대조군에 비해 유의적인 혈당저하 효과가 관찰되었으며, 혈장의 인슐린과 C-펩타이드 함량 역시 두릅, 오가피 및 느릅 열수추출물 급여시 STZ 투여에 의한 변화가 완화되었다. 혈장 중의 알부민 함량은 당뇨대조군이 정상군에 비하여 유의적으로 감소되었으며 실험식이 급여시 증가되는 경향이었다. 빌리루빈과 크레아티닌 함량은 STZ 투여로 유의적으로 증가되었으나 실험식이를 급여한 모든 군에서 유의적으로 감소되었다. 두릅, 오가피 및 느릅 열수추출물은 당뇨 유발로 증가된 혈장의 AST, ALT, ALP 및 LDH의 활성을 유의적으로 억제하였다. 따라서 두릅나무과 식물 중 두릅, 오가피 및 느릅의 열수추출물은 STZ 투여 흰쥐의 혈당강하 및 혈장바이오마커의 변화 개선에 효과적인 것으로 관찰되었다.

• Journal of Pharmaceutical Investigation
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• 제8권1호
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• pp.6-16
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• 1978

We obtained 4 kinds of extract fraction from Acanthopanacis Radicis Cortex and studied on the influence to the blood pressure of rabbit. These 4 fractions were obtained as follows; Fraction I was insoluble fraction by 99% ethanol from 80% methanol extract of Acanthopanacis Radicis Cortex, fraction II, precipitated fraction by ether from 99% ethanol soluble fraction of 80% methanol extract of Acanthopanaacis Radicis Cortex, fraction III, no precipitated fraction by ether from 99% ethanol soluble fraction of above 80% methanol extract and fraction IV, water extract of Acanthopanacis Radicis Cortex. All of fractions, when administered into ear-vein of rabbit, produced fall of blood pressure. Among these 4 fractions, although fraction III was not only the most potent but had the greatest efficacy, we observed the mechanism of hypotensive action of Acanthopanacis Radicis Cortex, making use of fraction II which was thought as a comparatively pure fraction. Hypotensive action of fraction II (APF II) was not affected by vagotominization but markedly inhibited by atropine. Pretreatment of bethanidine showed a tendency to weaken the depressor action of APF II, although it was not a significant result, but diphenhydramine did not influence APF II action. Phentolamine, guanethidine and chlorisondamine inhibited significantly the hypotensive action of APF II. APF II elicited the potentiation of norepinphrine pressor action dependent on the time-factor whereas it did not influence angiotesin pressor action. It is seemed that APF II exhibited hypotensive action, causing peripheral muscarinic-effect and centrally induced sympatholytic action.