• Journal of Microbiology and Biotechnology
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• 제6권6호
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• pp.456-460
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• 1996

Recombinant Pseudomonas lipase was used to study protein aggregation and adsorption upon in vitro refolding. Protein adsorption as well as aggregation was responsible for major side reactions upon in vitro refolding as a function of protein concentration. The optimal range of protein concentration was determined by the relative contribution of protein aggregation and adsorption. Above the optimal range, the yield of active lipase inversely correlated with protein aggregation, showing a competition between folding and aggregation. However, adsorption of protein rather than protein aggregation is thought to contribute as a major side reaction of the refolding process at sub-optimal concentrations at which the formation of aggregates should be more reduced. Protein aggregation was influenced by the amount of guanidine hydrochloride in the refolding solvent. The refolding temperature was a critical factor determining the extent of protein aggregation. The refolding yield was also affected by the dilution fold and dilution mode, which suggests that the refolding process might kinetically compete with the rate of mixing.

• 한국정보과학회논문지:데이타베이스
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• 제35권5호
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• pp.391-399
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• 2008

RSS는 웹 콘텐츠 배급을 위한 XML기반 포맷으로, 사용자는 RSS 피드 수집기를 통해 RSS 피드를 수집한다. RSS 피드를 효과적으로 수집하기 위해서는 RSS 피드에 대한 수집 정책이 필요하다. 본 논문은 RSS 피드 수집 시에 누락되는 포스팅을 최소화하기 위한 RSS 피드 수집 정책을 제안하고, 실험을 통해 제안한 수집 정책과 기존 수집 정책을 비교 분석하였다. 본 논문에서 제안한 수집 정책은 기존 수집 정책과 비교하여 6%의 수집 지연 증가로 23%의 수집 누락이 감소됨을 실험을 통하여 알 수 있었다.

• 정보처리학회논문지B
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• 제8B권6호
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• pp.669-674
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• 2001

병합 과정에 병합 상황을 반영하기 위해 상황 평가에 기반을 둔 병합(ASA) 방법이 제안되었다.[1]. 상황 평가 모델의 정수화된 출력은 병합을 위한 입력으로 사용된다. 상황 평가 모델의 정수화된 출력은 현재의 병합 상황 정도를 나타낸다. ASA 알고리즘은 최소값과 최대값 사이에서 기껏해야 몇 개의 병합 결과를 만들어 낸다. 결과적으로 ASA 방법은 최소값과 최대값 사이에서 보다 더 정교한 병합 결과를 갖는 응용 분야를 적절히 다룰 수 없다. 이러한 문제를 해결하기 위해 두 가지의 개선된 ASA (I-ASA) 방법을 제안한다. 이들 I-ASA 방법에서는 상황 평가 모델의 매개변수의 값이 실수 값이 되는 것이 허용되고, 최소값과 최대값 사이에서 연속된 병합 결과를 만들 수 있게 하기 위해 두 가지의 개선된 ASA 알고리즘을 제시한다. 이들 I-ASA 방법들은 정밀 병합과 근사 병합을 다룰 수 있다. 결과적으로, ASA 방법[1]과 비교할 때 제안된 I-ASA 방법들이 보다 더 정교한 병합 결과를 갖는 응용 분야를 적절히 다룰 수 있고, 또한 보다 범용적인 병합 분야에 사용될 수 있다는 관점에서 장점이 있다.

• Korea-Australia Rheology Journal
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• 제19권2호
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• pp.61-66
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• 2007

The aggregation characteristics of red blood cells (RBCs) are known as important factors in the microvascular flow system, and increased RBC aggregation has been observed in various pathological diseases, such as thrombosis and myocardial infarction. This paper describes a simple microfluidic device for measuring the RBC aggregation by integrating a microfluidic slit rheometry and laser-backscattering technique. While a decreasing-pressure mechanism was applied to the microfluidic rheometry, a syllectogram (the light intensity versus time) showed an initial increase and a peak caused by the high shear stress-induced disaggregation, immediately followed by a decrease in the light intensity due to RBC aggregation. The critical shear stress (CST) corresponding to the peak intensity was examined as a new index of the RBC aggregation characteristics. The CST of RBCs increased with increasing aggregation-dominating protein (fibrinogen) in the blood plasma. The essential feature of this design was the combination of the rheometric-optic characterization of RBC aggregation with a microfluidic chip, which may potentially allow cell aggregation measurements to be easily carried out in a clinical setting.

• 대한산업공학회지
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• 제40권5호
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• pp.520-525
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• 2014

When the PCF (Point Coordinated Function) MAC protocol is combined with the frame aggregation method to enhance the MAC performance in IEEE 802.11 wireless LANs, the formulae for the optimal frame aggregation level for best PCF MAC performance were derived in our previous study. We extend the formulae for the PCF protocol to derive the optimal frame aggregation level for the connectivity-based multipolling MAC protocol in IEEE 802.11 wireless LANs. By simulations, we compare the performances of IEEE 802.11 wireless LANs with the optimal and random frame aggregation levels. Compared with the random frame aggregation level, the optimal frame aggregation level significantly improves the performance of IEEE 802.11 wireless LANs.

• 약학회지
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• 제43권6호
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• pp.802-808
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• 1999

Vibrio vulnificus cytolysin has been incriminated as one of the important virulence determinants in V. vulnificus infection. In the present study, the effects of Vibrio vulnificus cytolysin on platelets were examined. Vibrio vulnificus cytolysin induced platelet aggregation and increased intracellular calcium concentration ($[Ca^{2+}]_i$) of rat platelets. These effects were abolished in $Ca^{2+}-free$ buffer (2 mM EGTA). Cytolysin also potentiated ADP-and collagen-induced platelet aggregation. Lanthanum (2 mM) inhibited cytolysin-diduced platelet aggregation. However, another $Ca^{2+}$ channel blockers, verapamil ($20{\;}{\mu}M$) or mefenamic acid ($20{\;}{\mu}M$) did not block cytolysin-induced platelet aggregation. Osmotic protectants, sucrose (50 mM) and raffinose (50 nM) suppressed platelet aggregation by 35.9% and 63.4%, respectively. V. vulnificus cytolysin increased membrane conductances of platelet membranes. These results suggest that cytolysin-induced platelet aggregation is mediated via lanthanum sensitive-calcium influx which resulted from the pore formation by V. vulnificus cytolysin.

• 한국정보통신학회논문지
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• 제13권6호
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• pp.1175-1180
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• 2009

차세대 무선랜 표준인 IEEE 802.11n에서는 MAC 계층에서의 처리율을 향상시키기 위해서 프레임 집약 (Frame Aggregation) 기법을 제안하고 있다. IEEE 802.11n의 프레임 집약 기법은 MSDU (MAC Service Data Unit) 단위의 집약 기법 (Aggregate MSDU: A-MSDU)와 MPDU (MAC Protocol Data Unit) 단위의 집약 기법 (Aggregate MPDU: A-MPDU)의 두 가지 기법을 제안하고 있다. 본 논문에서는 A-MSDU와 A-MPDU를 결합한 2단계 프레임 집약 (Two-Level Frame Aggregation) 기법의 성능을 분석한다. 성능 분석을 위해 마르코브 체인에 기반하여 2단계 프레임 집약 기법의 처리율을 모델링한 뒤 이를 이용하여 노드의 수와 채널 상태 등에 따른 프레임 집약 기법의 처리율 변화 추이를 분석한다. 그리고 분석 결과에 기반하여 최적의 프레임 집약 기법을 설계하기 위한 방법을 제시한다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제3권2호
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• pp.209-218
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• 2009

In a wireless sensor network(WSN) each sensor node deals with numerous sensing data elements. For the sake of energy efficiency and network lifetime, sensing data must be handled effectively. A technique used for this is data aggregation. Sending/receiving data involves numerous steps such as MAC layer control packet handshakes and route path setup, and these steps consume energy. Because these steps are involved in all data communication, the total cost increases are related to the counts of data sent/received. Therefore, many studies have proposed sending combined data, which is known as data aggregation. Very effective methods to aggregate sensing data have been suggested, but there is no means of deciding how long the sensor node should wait for aggregation. This is a very important issue, because the wait time affects the total communication cost and data reliability. There are two types of data aggregation; the data counting method and the time waiting method. However, each has weaknesses in terms of the delay. A hybrid method can be adopted to alleviate these problems. But, it cannot provide an optimal point of aggregation. In this paper, we suggest a stochastic-based data aggregation scheme, which provides the cost(in terms of communication and delay) optimal aggregation point. We present numerical analysis and results.

• 상하수도학회지
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• 제27권5호
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• pp.571-579
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• 2013

The aggregation behaviors of silver nanoparticles (AgNPs) and titanium dioxide ($TiO_2$) nanoparticles were investigated. Time-resolved dynamic light scattering (DLS) was used to study the initial aggregation of AgNPs and $TiO_2$ over a range of mono (NaCl) and divalent ($CaCl_2$) electrolyte concentrations. The effects of pH, initial concentration of NPs and natural organic matters (NOM) on the aggregation of NPs were also investigated. The aggregation of both nanoparticles showed classical Derjaguin-Landau-Verwey-Overbeek (DLVO) type behavior. Divalent electrolyte was more efficient in destabilize the AgNPs and $TiO_2$ than monovalent electrolyte. The effect of pH on the aggregation of AgNPs was not significant. But the aggregation rate of $TiO_2$ was much higher with increasing pH. Higher NPs concentration leads to faster aggregation. Natural organic matter (NOM) was found to substantially hinder the aggregation of both AgNPs and $TiO_2$. This study found that the aggregation behavior of AgNPs and $TiO_2$ are closely associated with environmental factors such as ionic strength, pH, initial concentration of NPs and NOM.

• 약학회지
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• 제46권6호
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• pp.441-447
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• 2002

It has been well known that vanadium shows various physiological and pharmacological properties such as an insulin-mimetic effect. In view of the reported toxic effects there is the problems that the safety margin is narrow because of its strong toxicity, Vanadate was tested for its ability to cause blood aggregation. Although vanadate or $H_2O$$_2$ alone had little effect on platelet aggregation, treatment of vanadate and $H_2O$$_2$ together induced platelet aggregation indicated that it was occurred by pervandate or hydroxyl radical produced from the reaction of vanadate and $H_2O$$_2$. It was dependent on extracellular $Ca^{2+}$ion. Platelet aggregation caused by vanadate and $H_2O$$_2$ was inhibited by ascorbic acid, tocopherol, catalase, mannitol, and Tiron. In contrast to vanadate, vanadium yeast prepared by uptaking vanadate in yeast cells did not induce platelet aggregation in the presence of $H_2O$$_2$.>.