• Journal of Environmental Policy
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• v.9 no.1
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• pp.83-107
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• 2010

Urbanized environments are constructed to estimate peak flow and cost savings in response to possible BMP allocation at a watershed scale. The main goal is to explore the proper allocation of sub-watershed level BMPs for peak flow attenuation at a watershed scale. Since several individual site scale BMPs work as a form of aggregated BMPs at a sub-watershed scale, it is a question as to how to properly allocate the sub-watershed level BMPs at a watershed scale. The Hydrological Simulation Program-FORTRAN (HSPF) is set up for a hypothetically urbanized watershed. A peak flow is determined to be the primary variable of interest and targeted to characterize the spatial distribution of aggregated BMPs. Construction cost of a regional pond forms the basis of the economic valuation. The results indicate that when total size of BMPs is constant in the entire watershed, (1) it is most effective to have aggregated BMPs in some upper sub-watersheds while the BMPs in either the mainstem sub-watershed or a single sub-watershed are the least effective choices for peak flow attenuation at a watershed scale; (2) savings exist between allocation differences and reduced peak flow increases cost savings. The largest saving is found in the strategy of aggregated BMPs in some upper sub-watersheds. These findings, however, call for follow-up site specific case studies revisiting the watershed scale impacts of BMP allocation. Then, it will be argued that location and extent of decentralization are considerable policy variables for an alternative stormwater management policy at a watershed scale.

• International Journal of Oral Biology
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• v.30 no.3
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• pp.99-103
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• 2005

Bone morphogenetic proteins (BMPs), members of a large group of TGF-beta family, are important molecular regulators of morphogenesis of numerous tissues and organs, including bones and teeth. Most BMPs are capable of inducing bone formation in vivo and therefore are of considerable clinical interest for regenerating mineralized tissues. Recently, we have developed a method to culture cells from human cementum (human cementum-derived cells, HCDCs). HCDCs, when attached to synthetic hydroxyapatite/tricalcium phosphate (HA/TCP) ceramic and transplanted into immunodeficient mice, formed histologically identifiable cementum-like tissue. Since it is unclear to what extent BMPs are involved in cementogenesis, the aim of this study was to establish which BMPs are expressed by cementogenic HCDCs and whether the expression of BMPs is related to the degree of cellular differentiation in vitro. HCDCs were maintained in growth medium (DMEM/F12 supplemented with 10% FBS) until confluent (proliferation stage). Upon reaching confluence, cells were incubated in the differentiation medium (DMEM/F12 medium containing 10% FBS and 50 mg/ml ascorbic acid) for 14 days (differentiation stage). Next, HCDCs were incubated in mineralization medium (DMEM/F12, 50 mg/ml ascorbic acid, 2.5 mg/ml of ITS (insulin-transferrinselenium), 5 mM beta-glycerophosphate and $10^{-8}M$ dexamethasone) for another 14 days (mineralization stage). At the end of each differentiation stage, total RNA was isolated and evaluated for BMPs (2 through 8) expression by employing real time RT-PCR. HCDCs expressed most of BMPs examined except BMP-7 and BMP-8. Furthermore, on average, the highest levels of BMPs were expressed at the earlier differentiation stage, prior to the initiation of mineralization in vitro. These results indicate that several BMPs are expressed during cementoblastic differentiation and suggest that BMPs may be involved in the homeostasis of human cementum.

• BMB Reports
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• v.44 no.10
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• pp.619-634
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• 2011

Bone morphogenetic protein (BMP) signaling in diseases is the subject of an overwhelming array of studies. BMPs are excellent targets for treatment of various clinical disorders. Several BMPs have already been shown to be clinically beneficial in the treatment of a variety of conditions, including BMP-2 and BMP-7 that have been approved for clinical application in nonunion bone fractures and spinal fusions. With the use of BMPs increasingly accepted in spinal fusion surgeries, other therapeutic approaches targeting BMP signaling are emerging beyond applications to skeletal disorders. These approaches can further utilize next-generation therapeutic tools such as engineered BMPs and ex vivo-conditioned cell therapies. In this review, we focused to provide insights into such clinical potentials of BMPs in metabolic and vascular diseases, and in cancer.

• Journal of Periodontal and Implant Science
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• v.39 no.sup2
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• pp.279-286
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• 2009

Purpose: Gene therapy (ex vivo) has recently been used as a means of delivering bone morphogenetic proteins (BMPs) to sites of tissue regeneration. In the present study, we investigated the effect of co-transduction of adenoviruses expressing BMP-2 and BMP-7 on osteogenesisof C2C12 cells in vitro. Methods: A replication-defective human adenovirus 5 (Ad5) containing a cDNA for BMPs in the E1 region of the virus (Ad5BMP-2 and Ad5BMP-7) was constructed by in vivo homologous recombination. Functional activity of Ad5BMP-2 and Ad5BMP-7 were evaluated in mouse stromal cells (W20-17cells). C2C12 cells are transduced with various MOI (multiplicity of infection) of Ad5BMP-2 and Ad5BMP-7 to assess most effective and stable titer. Based on this result, C2C12 cells were transduced with Ad5BMP-2 and Ad5BMP-7 alone or by combination. BMPs expression, alkaline phosphatase (ALPase) activity, cell proliferation, and mineralization were assessed. Results: Ad5BMP-2 and Ad5BMP-7 are successfully transduced to W20-17 cells, and secreted BMPs stimulated cell differentiation. Also, C2C12 cells transduced with Ad5BMPs showed expression of BMPs and increased ALPaseactivity. In all groups, cell proliferation was observed over times. At 7days, cells co-transduced with Ad5BMP-2 and Ad5BMP-7 showed lower proliferation than the others. C2C12 cells co-transduced with Ad5BMP-2 and Ad5BMP-7 had greater ALPaseactivity than that would be predicted if effect of individual Ad5BMPs were additive. Little mineralized nodule formation was detected in cells transduced with individual Ad5BMPs. In contrast, Ad5BMP-2 and Ad5BMP-7 combination stimulated mineralization after culturing for 10 days in mineralizing medium. Conclusions: Present study demonstrated that adenoviruses expressing BMPs gene successfully produced BMPs protein and these BMPs stimulated cells to be differentiated into osteoblastic cells. In addition, the osteogenic activity of Ad5BMPs can be synergistically increased by co-transduction of cells with Ad5BMP-2 and Ad5BMP-7.

• Journal of The Korean Society of Agricultural Engineers
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• v.51 no.5
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• pp.87-94
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• 2009

The objectives of this paper were to estimate cell based pollutant loadings for total maximum daily load (TMDL) programs and to evaluate the applicability of the agricultural nonpoint source (AGNPS) model for an intensive agricultural watershed in Korea. The model was calibrated and validated at a watershed of 384.8 ha of drainage area using the observed data from 1996 through 2000 in terms of runoff, suspended solid, total nitrogen, and total phosphorus on a hourly basis. Analysis of spatial variations of pollutant loadings for rainfall frequencies of various intensities and durations were conducted. In addition, the validated model was applied to estimated the TMDL removal efficiency for best management practices (BMPs) scenarios which were selected by taking into account the pollutant characteristics of the study watershed. The model can help to understand the problems and to find solutions through landuse changes and BMPs. Thus, the method used for this study was able to identify TMDL quantitatively as well as qualitatively for various sources pollution that are spatially dispersed. Also it provides an assessment of the impact of BMPs on the water bodies studied, allowing the TMDL programs to be complemented more effectively.

• International Journal of Industrial Entomology and Biomaterials
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• v.27 no.1
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• pp.159-165
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• 2013

Bone morphogenetic proteins (BMPs) belong to the transforming growth factor (TGF-${\beta}$) superfamily and are involved in osteoblastic differentiation. The largest TGF-${\beta}$ superfamily subgroup shares genetic homology with human BMPs (hBMPs) and silkworm decapentaplegic (dpp). In addition, hBMPs are functionally interchangeable with Drosophila dpp. Bombyx mori dpp may induce bone formation in mammalian cells. To test this hypothesis, we synthesized the 1,285-base pairs cDNA of full-length B. mori dpp using total RNAs obtained from the fat body of 3-day-old of the $5^{th}$ instar larvae and cloned the cDNA into the pCEP4 mammalian expression vector. Next, B. mori dpp was expressed in C3H10T1/2 cells. The target cells transfected with the pCEP4-Bm dpp plasmid showed biological functions similar to those of osteogenic differentiation induction growth factors such as hBMPs. We determined the relative mRNA expression rates of Runt-related transcription factor 2 (RUNX2), osterix, osteocalcin, and alkaline phosphatase (ALP) to validate the osteoblast-specific differentiation effects of B. mori dpp by performing quantitative real-time RT-PCR. Interestingly, mRNA expression levels of the 3 marker genes except RUNX2, in cells expressing B. mori dpp were much higher than those in control cells and C3H10T1/2 cells transfected with pCEP4. These results suggested that B. mori dpp signaling regulates osterix expression during osteogenic differentiation via RUNX2-independent mechanisms.

• Journal of Korean Society on Water Environment
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• v.28 no.3
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• pp.375-383
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• 2012

Soil erosion has been emphasized as serious environmental problem affecting water quality in the receiving waterbodies. Recently, Best Management Practices (BMPs) have been applied at a field to reduce soil erosion and its effectiveness in soil erosion reduction has been monitored with various methods. Although monitoring at fields/watershed outlets would be accurate way for these ends, it is not possible at some fields/watersheds due to various limitations in direct monitoring. Thus modeling has been suggested as an alternative way to evaluate effects of the BMPs. Most models, which have been used in evaluating hydrology and water quality at a watershed, could not reflect rainfall intensity in runoff generation and soil erosion processes. In addition, source codes of these models are not always public for modification/enhancement. Thus, runoff-sediment evaluation system using hourly rainfall data and vegetated filter strip (VFS) evaluation module at field level were developed using open source MapWindow GIS component in this study. This evaluation system was applied to Bangdongri, Chuncheonsi to evaluate its prediction ability and VFS module in this study. The NSE and $R^2$ values for runoff estimation were 0.86 and 0.91, respectively, and measured and simulated sediment yield were 15.2 kg and 16.5 kg indicating this system, developed in this study, can be used to simulate runoff and sediment yield with acceptable accuracies. Nine VFS scenarios were evaluated for effectiveness of soil erosion reduction. Reduction efficiency of the VFS was high when sediment inflow was small. As shown in this study, this evaluation system can be used for evaluation BMPs with local rainfall intensity and variations considered with ease-of-use GIS interface.

• Journal of Wetlands Research
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• v.20 no.4
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• pp.304-313
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• 2018

Agricultural land reclamation in Saemangeum tidal land project is mostly planned to be completed by 2020. Irrigation water for the land is required to be prepared by that time. However, water quality for the irrigation sources is barely meet the target concentration. This paper described the reduction effect of and policy consideration for best management practices (BMPs) which were fertilizer prescription by soil test (SO#1), mixed application of SO#1 and 3 (SO#2), drainage gate control (SO#3), time-release fertilizer application (SO#4), and control (CT). Reduction of paddy runoff was relatively higher in SO#3 (25%) and SO#1 (27%) while lower in SO#4 (9%) and SO#2 (7%) than that in CT. In addition, farmers promised to follow the BMP guidelines but they didn't because of the several problems caused for the BMPs implementation. Thus, it recommended developing an automated control of irrigation gate and paddy water depth and supporing farmers for NPS pollution control and irrigation water reduction.

• Journal of Korean Society on Water Environment
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• v.26 no.3
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• pp.387-398
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• 2010

SWAT model would be applied to evaluate the pollutant removal capacity with various best management practices (BMPs) in Kyongan stream watershed which plays an important role in water quality conservation and improvement of Paldang reservoir. The methods for the representation of various BMPs scenarios with SWAT is developed and evaluated. Riparian buffer strip, agricultural conservation practices to reduce fertilizer, sediment, and nutrients occurring from farm field (Grassed swale, Contour farming/Parallel terrace, Field border, Farm retention pond, Grade stabilization structure), and washland such as wetland and pond to extend detention and improve water quality are represented in SWAT. And to represent the expansion of existing Waste Water Treatment Plants (WWTPs) in Soil and Water Assessment Tool (SWAT), reduction effect for point source pollutants was simulated. As the result of simulation, the removal rates of SS, TN, TP from scenarios of Kyongan stream watershed are the average annual SS yield by 5.2% to 69.2%, the average annual TN yield by 0.5% to 26.3%, and the average annual TP yield by 1.3% to 32.5%, respectively. This study has demonstrated that the SWAT is a very reliable and useful water quality and quantity assessment tool, and the BMPs representation in SWAT for watershed management is able to effectively simulate in Kyongan Stream watershed.

• Journal of Korean Society on Water Environment
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• v.34 no.4
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• pp.382-390
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• 2018

In this study, the LIDMOD3 was developed to design and evaluate low impact development (LIDMOD). In the same fashion, the LIDMOD3 employs a curve number (NRCS-CN) method to estimate the surface runoff, infiltration and event mean concentration as applicable to pollutant loads which are based on a daily time step. In these terms, the LIDMOD3 can consider a hydrologic soil group for each land use type LID-BMP, and the applied removal efficiency of the surface runoff and pollutant loads by virtue of the stored capacity, which was calculated by analyzing the recorded water balance. As a result of Model development, the LIDMOD3 is based on an Excel spread sheet and consists of 8 sheets of information data, including: General information, Annual precipitation, Land use, Drainage area, LID-BMPs, Cals-cap, Parameters, and the Results. In addition, the LIDMOD3 can estimate the annual hydrology and annual pollutant loads including surface runoff and infiltration, the LID efficiency of the estimated surface runoff for a design rainfall event, and an analysis of the peak flow and time to peak using a unit hydrolograph for pre-development, post-development without LID, and as calculated with LID. As a result of the model application as applied to an apartment, the LIDMOD3 can estimate LID-BMPs considering a well spatical distributed hydroloic soil group as realized on land use and with the LID-BMPs. Essentially, the LIDMOD3 is a screen level and simple model which is easy to use because it is an Excel based model, as are most parameters in the database. This system can be expected to be widely used at the LID site to collect data within various programmable model parameters for the processing of a detail LID model simulation.