• Title/Summary/Keyword: BcL-xL

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Variation of Nuclear DNA Content in Interspecific Allium cepa L.×A. fistulosum L. hybrids and Their Successive Backcross Lines (양파와 파간의 종간잡종 F1과 여교잡계통의 핵 DNA 함량)

  • Kim, Cheol-Woo;Kim, Hwa-Young;Lee, Eul-Tai;Choi, In-Hu;Bang, Jin-Ki
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.463-467
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    • 2009
  • Interspecific hybrid plants between Allium cepa L. (2n=2X=16) and A. fistulosum L. (2n=2X=16)and their backcross lines were developed by artificial pollination in order to introduce new desirable characters of A, cepa to A. fistulosum. The 2C nuclear DNA content has been estimated by flow cytometry in 5 Allium fistulosum inbreed lines, 2 interspecific hybrid lines of A. cepa${\times}$A. fistulosum and 34 their backcross lines $BC_1F_1$ to $BC_2F_2$, using propidium iodide (PI) as a fluorescence dye. Estimated 2C DNA values ranged from 22.2 pg to 23.7 pg in 5 A. fistulosum inbreed lines, 37.9 pg in F1 hybrid between A. cepa and A. fistulosum, 24.3 pg to 27.3 pg in 7 backcross lines in $BC_1F_1$, 21.9 pg to 24.4 pg in 9 $BC_1F_2$, 22.9 pg to 25.1 pg in 14 $BC_2F_1$, 22.6 pg to 23.4 pg in 4 $BC_2F_2$. This study showed mean 2C nuclear DNA content of $F_1$ hybrid was higher than their backcross progeny lines, while it was lower than female parental line, A. cepa (2C DNA=33.2 pg). Mean 2C DNA content of backcross lines, $BC_1F_1$ to $BC_2F_2$ was not significantly different but their 2C DNA contents in the more progress generation from $BC_1F_1$ to $BC_2F_2$ were reduced.

Design of transistor oscillator for X-band application using a pair of L-shaped monopole slot resonator (한 쌍의 L-형 모노폴 슬롯 공진기를 이용한 X-밴드 트랜지스터 발진기 설계)

  • Lee, Yeong-min;Lee, Young-soon
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.21 no.1
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    • pp.107-114
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    • 2021
  • In this paper, a planar transistor oscillator for X-band using a newly proposed L-shaped monopole slot resonator is proposed. For planar design, an L-shaped monopole slot with an open-end is used as a resonator for a transistor oscillator. As a result of the simulated design of the resonator in three stages, a high Q value of 1169.84 and a high insertion loss of 49.934 dB were identified. The results of the final design and manufactured oscillator measurements confirmed that the oscillation output is greater than 7 dBm and has good phase noise characteristics of -58 dBc/Hz at 100 kHz offset. The proposed oscillator is planar and has the advantage of being directly applicable to microwave integrated circuit technology. It also has the advantage of being able to reduce its size as it can only be implemented in microstrip form without additional devices such as metal cavities and tuning screws in 3D structures, as in the case of a DRO (dielectric resonance oscillator).

Effects of Rubus Coreanus Miquel on the Apoptosis of U937 Cells (복분자가 U937세포의 apoptosis에 미치는 효과)

  • Lee, Kwang-Kyu;Han, Jong-Hyun;Oh, Chan-Ho
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.6
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    • pp.1404-1408
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    • 2009
  • Methanol extracts of Rubus Coreanus Miquel (RCM) were found to exhibit apoptosis induction of U937 human histiocytic leukemia cells. Treatment of RCM exerted strong cytotoxicity against U937 human leukemia cells. RCM induced apoptosis of U937 leukemia cells in a dose dependent manner. Nitric oxide (NO) production was increased in RCM-treated RAW264.7 macrophage cell line. RCM increased the p53 and $NF{\kappa}B$ gene and decreased the $I{\kappa}B$ gene expression in U937 cells. RCM also increased the $NF{\kappa}B$ protein expression, but decreased the PCNA and BcL-xL protein expression in cultured U937 cells. These data suggest that RCM are effective on the apoptosis induction of human leukemia cells and anti-cancer properties.

THE HARMONIC ANALYSIS ASSOCIATED TO THE HECKMAN-OPDAM'S THEORY AND ITS APPLICATION TO A ROOT SYSTEM OF TYPE BCd

  • Trimeche, Khalifa
    • Korean Journal of Mathematics
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    • v.27 no.1
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    • pp.221-267
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    • 2019
  • In the five first sections of this paper we define and study the hypergeometric transmutation operators $V^W_k$ and $^tV^W_k$ called also the trigonometric Dunkl intertwining operator and its dual corresponding to the Heckman-Opdam's theory on ${\mathbb{R}}^d$. By using these operators we define the hypergeometric translation operator ${\mathcal{T}}^W_x$, $x{\in}{\mathbb{R}}^d$, and its dual $^t{\mathcal{T}}^W_x$, $x{\in}{\mathbb{R}}^d$, we express them in terms of the hypergeometric Fourier transform ${\mathcal{H}}^W$, we give their properties and we deduce simple proofs of the Plancherel formula and the Plancherel theorem for the transform ${\mathcal{H}}^W$. We study also the hypergeometric convolution product on W-invariant $L^p_{\mathcal{A}k}$-spaces, and we obtain some interesting results. In the sixth section we consider a some root system of type $BC_d$ (see [17]) of whom the corresponding hypergeometric translation operator is a positive integral operator. By using this positivity we improve the results of the previous sections and we prove others more general results.

Gene Location for "Gamadiness" in Rice(Oryza sativa L.) (벼 "Gamadiness"특성의 유전분석)

  • Shrestha,, G.L.;M. H. Heu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.2
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    • pp.128-135
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    • 1984
  • Gamadi, a native rice cultivar from Nepal in which the panicle remains enclosed within its flag leaf sheath upto maturity, was crossed with different genetic marker testers of 12 linkage groups in order to analyze its linkage relationship. The results obtained from the experiment were summarized as follows: Normal segregations of all the genetic marker genes used in this experiment viz Cl, wx and Pla of linkage group I, Pn, Rd and Pub of linkage group III, and lg, g, Ps, gh, Hla, la, nl, bl, be and gl of linkage groups II, IV, V, VI, VII, VIII, IX, X, XI, and XII respectively confirmed the previous results, and also strongly indicated that the genetic constituent of the Gamadi and marker testers is same. 'Gamadiness' (the panicle enclosing character) was controlled by two complementary dominant genes with the segregation ratio of 9 Gamadi to 7 normal panicle-exserting types. These genes have been temporarily proposed as G-a and G-b for gamadiness. G-a gene was found to be linked with the neckleaf gene (nl) of linkage group Ⅸ with the crossover value of 0.3733$\pm$0.027. G-b gene appeared to be associated with the brittle culm gene (bc) of the linkage group XI with the crossover value of 0.2725$\pm$0.061.TEX>0.061.

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Design and Implementation on Frequency Synthesizer Qualification Model Level for SAR payload (위성 레이다용 QM급 주파수합성기 설계 및 제작)

  • Kim, Dongsik;Kim, Hyunchul;Heo, John;Kim, Wansik
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.20 no.3
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    • pp.9-14
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    • 2020
  • In this paper, Qualification Model of frequency synthesizer is designed for X-band SAR system and performed electrical and environment test. Designed frequency synthesizer generate 13.65 GHz with very low phase noise performance. The integrated phase noise from 10Hz to 1MHz is -37.91 dBc. IRF performances are analyzed according to phase noise and jitter. Also, thermal and structure analysis are achieved for stable operation in space environment. Designed frequency synthesizer is consist of 2 modules of 6U size and generate L-band, C-band, Ku-band. The result of this study would enhance the design ability of RF module and help the frequency synthesizer design for SAR payload system.

Production and Molecular Cytogenetic Identification of Wheat-Barley Hybrids and Translocations

  • Lang, Marta-Molnar;Gabriella Linc;Jozsef Sutka
    • Journal of Plant Biotechnology
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    • v.1 no.1
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    • pp.8-12
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    • 1999
  • New winter wheat winter barley hybrids were produced (Mv9 kr1 Igri, Mv9 kr1 Osnova, Asakazekomugi Manas). The wheat-barley hybrids showed entire male sterility and were multiplied in tissue culture. Chromosome configurations were studied with GISH in meiosis in the Mv9 krl x Igri hybrid and in its progenies multiplied in vitro. Chromosome pairing between wheat and barley has been observed in some cells in the hybrids multiplied in vitro. Backcross plants with 43 and 44 chromosomes were selected with the aim of developing new wheat-barley addition lines. Wheat-barley translocations were demonstrated with GISH in backcross progenies originating from in vitro regenerated wheat (Triticum aestivum L. cv. Chinese Spring) x barley (Hordeum vulgare L. cv. Betzes) hybrids. Five different translocations were observed. Sequential N-banding and GISH analyses were performed to further identify the translocations. The N-banding pattern of the Robertsonian translocation suggests that this chromosome consists of the short arm of barley chromosome 4H translocated to the long arm of 2B of wheat. Plants with four different homozygous translocations were selected from the following BC2F3 generation.

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Ultra Low Noise Hybrid Frequency Synthesizer for High Performance Radar System (고성능 레이다용 저잡음 하이브리드 주파수합성기 설계 및 제작)

  • Kim, Dong-Sik;Kim, Jong-Pil;Lee, Ju-Young;Kang, Yeon Duk;Kim, Sun-Ju
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.48 no.1
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    • pp.73-79
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    • 2020
  • Modern radar system requires high spectral purity and low phase noise characteristics for very low RCS target detection and high resolution SAR (Synthetic Aperture Radar) image. This paper presents a new X-band high stable frequency synthesizer for high performance radar system, which combines DAS (Direct Analog Synthesizer) and DDS (Direct Digital Synthesizer) techniques, in order to cope with very low phase noise and high frequency agility requirements. This synthesizer offers more than 10% operating bandwidth in X-band frequency and fast agile time lower than 1 usec. Also, the phase noise at 10kHz offset is lower than -136dBc/Hz, which shows an improvement of more than 10dB compared to the current state of art frequency synthesizer. This architecture can be applied to L-band and C-band application as well. This frequency synthesizer is able to used in modern AESA (Active Electronically Scanned Array) radar system and high resolution SAR application.

Effect of Small Dose of Radiation on Induction of Apoptosis in Murine Tumors (마우스 종양에서 저선량 방사선이 Apoptosis의 유도에 미치는 영향)

  • Seong, Jin-Sil;Pyo, Hong-Ryull;Chung, Eun-Ji;Kim, Sung-Hee;Suh, Chang-Ok
    • Radiation Oncology Journal
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    • v.17 no.4
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    • pp.307-313
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    • 1999
  • Purpose : To investigate the Presence of adaptive response by low dose radiation in murine tumors in relation to radiation induced apoptosis as well as related mechanism. Materials and Methods : Syngeneic murine tumors, OCa-I and HCa-l, were given 0.05 Gy pretreatment followed by therapeutic dose of 25 Gy radiation. Induction of apoptosis was analyzed for each treatment group. Regulating molecules of apoptosis, p53, Bcl-2, Bax, Bcl-X, were also analyzed by Western blotting. Results : In 0.05 Gy pretreatment group of OCa-I, 25 Gy-induced apoptosis per 1000 cells was 229, which was estimated at $30\%$ lower level than the expected (p<0.05). In contrast, this reduction in radiation induced apoptosis was not seen in HCa-l. In the expression of apoptosis regulating molecules, p53 increased in both tumors in response to radiation. Bcl-2 and Bax did not show significant change in both tumors however, the expression of Bcl-2 surpassed that of Bax in 0.05 Gy pretreatment group of OCa-l. Bcl-X was not expressed in OCa-l. In HCa-l, Bcl-X showed increased expression even with 0.05 Gy. Conclusion : Adaptive response by low dose radiation Is shown in one murine tumor, OCa-l, in relation to radiation induced apoptosis. Apoptosis regulating molecules including Bcl-2/Bax and Bcl-X, appear to related. This study shows an evidence that adaptive response is present, but not a generalized phenomenon in vivo.

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Nature of Gene Action for Duration of Grain filling in Crosses of Winter and Spring Wheats(Triticum aestivum L. em Thell) (춘.추 파성 소맥품종간 교잡에서 등숙기간을 지배하는 유전자 작용에 관한 연구)

  • Byung Han, Choi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.2
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    • pp.131-139
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    • 1985
  • Breeders have concerned with the nature of gene action controlling the duration of grain filling period to combine early maturity and acceptable grain yield to fit wheat into multiple cropping systems. The 4 x 4 complete diallel set of F$_1$, F$_2$ and 1/2 (BC$_1$ + BC$_2$) in crosses of winter and spring wheat cultivars was made to determine the nature of gene action involved for duration of grain filling period. Using the Jinks-Hayman model, no maternal effects were noted nor were any non-allelic interactions observed for total duration of grain filling and lag period. The actual grain filling period was influenced to some degree by such interactions. The spring cultivars Red Bobs and Siete Cerros also appeared to have more dominant genes for longer total duration of grain filling and lag period. In contrast, the winter parents Yamhill and Hyslop had more dominant genes for the longer actual grain filling period. The genes appeared to be independently distributed among the parents.

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