• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.3
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• pp.826-828
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• 2003

The effects of crude extract and bioactive fractions from Brachyglottis monroi on biological activity were investigated. The crude ethanol extract inhibited the growth of the Gram positive bacterium Bacillus subtilis (ATCC Strain number 19659, 1 mm zone at 150 μg/disk) and the dermatophyte Trichophyton mentagrophytes (ATCC 28185, 2 mm zone at 150 μg/disk), and toxic to P388 tumor cells (IC50 23.96 μg/ml at 75 μg/disk). Cytotoxic activity was strongly showed by Fr. 6 (P388 IC50 19.67 μg/ml at 75 μg/disk).

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.1
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• pp.260-264
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• 2004

The effects of bioactive fractions from Brachyglottis monroi on biological activity were investigated. this bioactive subfraction 6-5 is the most cytotoxic to P388 murine leukaemia cell lines. A comparison of IC/sub 50/ values of these subfraction in cancer cell lines showed that their susceptibility to these subfractions decreased in the following order; Fr. 6-5 > Fr. 6-3 > Fr. 6-6 > Fr. 6-1 > Fr. 6-2 > Fr. 6-4 by the MTT method. Silica gel flash column chromatography concentrated the cytotoxic activity in subfraction 6-5 which eluted with 30% and 40% ethyl acetate : hexane gave a major bioactive (51 mg, P388 IC/sub 50/ 8,286 ng/mL at 7.5 ㎍/disc).

• Journal of dental hygiene science
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• v.3 no.2
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• pp.67-70
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• 2003

The biological effects of the crude extracts from medicinal plants, Brachyglottis monroi and Trichocolea hatcheri were investigated. The crude ethanol extract inhibited the growth of the Gram positive bacterium Bacillus subtilis (ATCC 19659, 1 mm inhibition zone at $150{\mu}g/disc$) and the dermatophyte Trichophyton mentagrophytes (ATCC 28185, 6 mm inhibition zone at $150{\mu}g/disc$), and was toxic to P388 murine leukaemia cells ($IC_{50}$ $23.96{\mu}g/mL$ at $75{\mu}g/disc$). B. monroi ethanol extract showed stronger antiviral activity than that of T. hatcheri against Herpes simplex Type I virus (ATCC VR 733) and Polio Type I virus (Pfizer vaccine strain) (50% activity, @ 5 mg/ml at $150{\mu}g/disc$). The crude ethanol extract of T. hatcheri showed stronger antimicrobial activity than that of B. monroi. However, this extract was inactive against P388 murine leukaemia cells.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.1
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• pp.230-233
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• 2005

The cytotoxic activity of $13(E)-labd-13-ene-8{\alpha}$, 15-diol (1), isolated from the ethanol extract of Brachyglottis monroi, was evaluated against human oral epithelioid carcinoma cells(KB).

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.141.1-141.1
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• 2003

The inhibitory effect of 13(E)-Labd-13-ene-8$\alpha$ 15-diol(1), isolated from the ethanol extract of Brachyglottis monroi, on the proliferation of human breast cancer MDA-MB231 cells was examined. Compound (1) at concentration as high as 16$\mu$/$m\ell$ has inhibited the proliferation of MDA-MB231 and this cytotoxic effect was increased in a time and dose-dependent manners. (omitted)

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.179.2-179.2
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• 2003

The cytotoxic activity of 13(E)-Labd-13-ene-8$\alpha$, 15-diol(1), isolated from the ethanol extract of Brachyglottis monroi was evaluated against tumor cell lines such as P388, SNU-C4 MDA-MB231, B 16 melanoma and A549 in vitro. By mean of spectral analysis particularly by the aid of various two dimensional NMR experiments, 1H-NMR and 13C-NMR signals of (1) was completely assigned, and thus the structure of (1) was established unambiguously. (omitted)

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.1
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• pp.250-253
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• 2004

The effects of crude extracts from medicinal plants on biological activity were investigated. The crude ethanol extract of H. paucistipula inhibited the growth of the Gram positive bacterium Bacillus subtilis ATCC 19659, (2 mm inhibition zone at 150 ㎍/disc) and the dermatophyte Trichophyton mentagrophytes ATCC 28185, (7 mm inhibition zone at 150 ㎍/disc), and toxic to P388 murine leukaemia cells ATCC CCL 46 P388D1, (IC/sub 50/ 2.48 ㎍/㎖ at 75 ㎍/disc). This crude ethanol extract of H. paucistipula is the strongest antimicrobial and cytotoxic activities against P388 murine leukaemia cells ATCC CCL 46 P388D1.

• Journal of Applied Biological Chemistry
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• v.56 no.1
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• pp.49-51
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• 2013

In a previous study, we reported that 13(E)-labd-13-ene-$8{\alpha}$,15-diol (13E) possesses antiviral and anticancer activities. In this study, the anticancer and antimicrobial activities of 13(E) were evaluated against 4 cancer cell lines and 6 bacteria. 13(E) showed inhibitory effect on a variety of cancer cell lines. The $IC_{50}$ values was 8.3-21.3 ${\mu}g/mL$. 13(E) was the most effective growth inhibitor of murine leukaemia cell lines P388, producing approximately 8.3 ${\mu}g/mL$ of $IC_{50}$ in the cytopathic effect (CPE) method. 13(E) also inhibited the growth of the gram-positive bacteria (Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes) and gram-negative bacteria (Vibrio parahaemolyticus, Escherichia coli and Salmonella enteritidis) with a range of minimum inhibitory concentration (MIC) values from 0.092 to 0.598 mg/mL and gram-negative bacteria were more sensitive to the compound (MIC, 0.092 mg/mL).