• Molecules and Cells
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• 제19권1호
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• pp.74-80
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• 2005

Stress is known to inhibit granule cell proliferation in the hippocampus. However, recent studies suggest that the commonly used dose of bromodeoxyuridine (BrdU) is insufficient to label all fractions of granule cells. Furthermore, stress-induced changes in BrdU availability may influence the labeling of newly born cells. To investigate whether changes in BrdU availability affect measurements of stress-induced granule cell proliferation, granule cell proliferation was assessed using injection of high doses of BrdU before and after restraint stress lasting 1 h. In addition, to determine whether stress-induced changes in plasma corticosterone levels were influenced by the BrdU, time-dependent changes in plasma corticosterone levels over 2 h after BrdU injection were compared with total accumulated plasma corticosterone levels [as determined by areas under the curve (AUC)]. Restraint stress significantly reduced the numbers of BrdU-labeled cells and clusters in the granule cell layer (GCL) of rats that received BrdU after stress, and decreases of similar magnitude were observed when the rats were given BrdU before stress. BrdU injection enhanced the stress-induced plasma corticosterone response, but there was no difference between the mean AUCs of plasma corticosterone levels of animals injected with BrdU before or after stress. These observations suggest that restraint stress decreases granule cell proliferation, and that this may be influenced by the extent and duration of plasma corticosterone increases rather than by changes in the availability of BrdU.

• International Journal of Oral Biology
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• 제33권2호
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• pp.59-64
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• 2008

This study was designed to investigate the changes in the properties of the neuronal setm cells or progenitor cells associated with age-related decline in neurogenesis of the hippocampal dentate gyrus (DG). Active whole cells cycle marker Ki67 (a marker of whole cell cycle)-positive and S phase marker bromodeoxyuridine (BrdU)-positive. Neural stem cells gradually were reduced in the hippocampal subgranular zone (SGZ) in an age-dependant manner after birth (from P1 month to P1 year). The ratio of BrdUpositivecells/Ki67-positive cells was gradually enhanced in an age-dependent manner. The ratio of Ki67-positive cells/accu-mulating BrdU-positive cells at 3 hrs after BrdU injection was injected once a day for consecutive 5 days gradually decreased during ageing. TUNEL- and caspase 3 (apoptotic terminal caspase)-positive cells gradually decreased in the dentate SGZ during ageing and immunohistochemical findings of glial fibrillary acid protein (GFAP) were not changed during ageing. NeuN, a marker of mature neural cells, and BrdU-double positive cells gradually decreased in an age-dependent manner but differentiating ratio and survival rate of cells were not changed at 4 wks after BrdU injection once a day for consecutive 5 days. The number of BrdU-positive cells migrated from the hippocampal SGZ into granular layer and its migration speed was gradually declined during ageing. These results suggest that the adult neurogenesis in the mouse hippocampal DG gradually decrease through reducing proliferation of neural stem cells accompanying with cells cycle change and reduced cells migration rather than changes of differentiation.

• 대한한의학회지
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• 제23권3호
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• pp.26-32
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• 2002

목적 : 본 연구에서는 알코올 독성에 대하여 흰쥐의 치상회에서 새로운 신경세포의 생성 및 nitric oxide synthase (NOS) 발현에 인삼이 미치는 영향을 5-bromo-2-deoxyuridine (BrdU) 면역 조직 화학법 및 nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) 조직화학법을 통해서 관찰하고자 한다. 방법 : 실험동물을 정상군, 인삼처치군, 알코올처치군 및 알코올-인삼 처치군으로 분류하여 각각의 실험군에 3일간 BrdU (50mg/kg)를 복강주사하였다. 인삼처치군은 30mg/kg 용량의 인삼 전탕액을 중완혈에 약침주사하였고, 알코올 처치군은 2 g/kg 용량의 알코올을 투여하였으며. 알코올-인삼 처치군은 2 g/kg 용량의 알코올 및 30mg/kg 용량의 인삼 전탕액을 투여한 후 각각의 BrdU 양성 세포수와 NADPH-d 양성세포수를 관찰하였다. 결과 : 알코올 투여군은 BrdU 양성세포 및 NADPH-d 양성세포 발현이 감소하였으나, 인삼 및 알코올 인삼처치군에서는 알코올 투여군에 비해서 모두 증가하였다. 결론 : 인삼은 알코올에 의해서 유발된 새로운 신경세포 생성의 감소에 대하여 보호효과가 있으며, 알코올에 의해서 부가적으로 영향 받는 산화질소는 세포생성 조절에 중요한 역할을 하는 것으로 사려된다.

• Parasites, Hosts and Diseases
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• 제31권2호
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• pp.83-90
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• 1993

간흡충에 감염된 담관은 확장되면서 상피세포의 수가 크게 증식하여 점막층이 두꺼워지고 과식증에 의해 점막의 단면이 유두상으로 관찰된다. Bromodeoxyuridine (BrdU)이 세포가 핵산을 복제할 때에 끼어 들어가는 성질을 이용하여, 이 연구에서는 담관 상피세포 중에서 합성기(Sphase)에 있는 세포의 분율을 파악하고자 하였다. 흰쥐에 간흡충의 피낭유충을 100 개씩 경구 감염시키고, 15주까지 간의 각 엽에 있는 담관의 조직절편을 만들어서 다세포군항체와 ABC complex를 이용한 염색을 실시하였다. 그 결과 충제가 들어 있는 담관에서는 감염 1주에 상피 세포의 2.9-10.2%가 염색에 양성이었고, 2주에 7.3-12.8%가, 5주에는 7.3-13.4%가, 15주에 8.4-14.8%가 BrdU를 함유하였다. 대조군 흰쥐나 실험군 동물의 감염되지 않은 담관의 경우에는 0-2.7%가 염색에 양성으로 나타났다. 염색된 상피세포는 주로 점막 상피세포층의 기저부에서 관찰되었다. 이 결과에 의하면 간흡충과 직접 접촉하는 담관의 상피세포가 뚜렷하게 증식하고 있어 충체의 어떠한 물리적인 또는 화학적인 자극이 있더라도 가까운 부위에서 주로 작용하고 있다고 판단하였다.

• 한국임상수의학회지
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• 제14권2호
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• pp.230-234
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• 1997

In vitro effect of cortisol on the proliferation of canine peripheral blood mononuclear cells (MNC) was examined. The MNC was isolated from peripheral blood by a gradient centrifugation with Picoll-Hypaque. The cell proliferation assayed using a noneradioactive 5-Bromo-2'-deoxy-uridine (BrdU) kit. The MNC proliferated well in response to either phrtobeRagg$]$utinin-p (PHA-P) or culture supernatant from MNC stimulated with PHA-p. However, these proliferative responses of MNC were not affected by addition of coitisol of 1 to 1,OOfl ng/ml. The addition of cortisol in MNC culture with either PHA-P or corture supernatBnt from MNC stimulated with PHA-P far 4 days wag not also influenced on the viabilities of cultured MNC. In conclusions it was able to assay the cell proliferation with BrdU instead of radioactive isotope e.g. tritiated thymidine (3H-TdR). These results suggested that cortisol does not at least influence on MNC proliferation in vitro.

• 대한치과교정학회지
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• 제26권4호
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• pp.359-371
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• 1996

교정력에 의한 성견의 치아이동시 발생하는 조직변화 중 치주인대 내에서의 세포활성도의 변화를 교정력의 크기와 기간에 따라 알아 보고자 하였다. 생후 1년 6개월된 성견 6마리를 5마리의 실험군과 1마리의 대조군으로 나누었고 실험군에서 하악 좌측에는 강한 힘 (250-300g)을, 하악 우측에는 약한 힘 (50-75g)을 제1소구치와 제2소구치 사이에 open coil spring으로써 적용하였다. 실험군의 성견을 각각 12시간, 24시간, 3일, 1주, 2주에 Bromodeoxyuridine(BrdU)을 주입하고 희생시켰다. 하악 제 1,2 소구치 부위의 조직을 채득하여 통법에 따라 파라핀 포매하였으며, H & E 염색과 항 BrdU 항체를 이용한 면역조직화학적 염색을 시행한 결과 다음과 같은 결론을 얻었다. 견인측에서의 치주인대 단절과 혈관확장은 12시간째에서 관찰되어 증가하다가 3일째 이후에는 감소되었는데 약한 힘을 준 경우보다 강한 힘을 준 경우에서 더 많았으며 압박측에서의 치주인대의 초자양변성과 파골세포 활성은 12시간째에서부터 관찰되어 3일째까지 증가하다가 7일째부터 감소하였는데 약한 힘을 준 경우보다 강한 힘을 준 경우에서 더 많이 나타났다. 대조군의 BrdU 발현은 구강상피와 치주인대의 섬유모세포에서 주로 많았고 골세포나 파골세포에서는 음성 반응을 보였으며 실험군의 BrdU 발현은 압박 측보다 견인측에서 많았으며 치경부쪽보다는 치근단쪽에서 더 많았다. 약한 힘을 준 경우에서는 BrdU 발현이 1일째에 가장 많이 나타나다가 감소되었고 강한 힘을 준 경우에서는 12시간째에서 최고에 달하다가 이후에는 감소되었으나 14일째에는 실험군과 대조군 간에 큰 차이가 없었다.

• 한국전문물리치료학회지
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• 제12권3호
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• pp.11-21
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• 2005

The present study was aimed at investigating the effect of swimming training on brain function after focal cerebral ischemia in rats. Therefore, this study was examined on neurogenesis in dentate gyrus of hippocampus using 5-bromo-2'-deoxyuridine (BrdU) to label proliferating cells and assessed the neurological response following focal cerebral ischemia in rats using neurological motor behavioral test. In an observer-blinded fashion, twenty male Sprague-Dawley (280~310 g, 7 weeks old) rats were divided into four groups: MCAO plus swimming group (ME, $n_1$=5), MCAO plus control group (MC, $n_2$=5), SHAM plus swimming group (SE, $n_3$=5), SHAM plus control group (SC, $n_4$=5). The results of this study were as follows: 1) The limb placing time before and after swimming in the ME group were significantly longer than the MC group (p<.05), the SE group were significantly longer than the SC group (p<.01). 2) The balance beam scores before and after swimming in the ME group was higher than the SE group, the MC group was higher than the SC group but was not significantly different (p>.001). 3) The foot fault index before and after swimming training in ME group was significantly lower (i.e., improved) than the MC group (p<.001) and the SE group (p<.001), the SE group was significantly lower (i.e., improved) than the SC group (p<.001). 4) The mean number of BrdU-positive cells in the dentate gyrus in the ME group was significantly higher than the MC group (p<.001) and the SE group (p<.01). The MC group and the SE group was significantly higher than the SC group (p<.001). 5) There was significantly correlation between limb placing time and number of BrdU-positive cells on swimming training, there was positive correlation (r=.807, p<.0001) and between foot fault index and BrdU-positive cells number, there was negative correlation (r=-.503, p<.05). However, between balance beam scores and BrdU-positive cells number, there was no correlation. In conclusion, the present study demonstrates that the role of swimming training improves behavioral motor function probably by enhancing cell proliferation in that hippocampus. This study provides a model for investigating the stroke rehabilitation that underlies neurogenesis and functional ability.

• 대한감각통합치료학회지
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• 제2권1호
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• pp.21-32
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• 2004

Objective : Effect of treadmill exercise on hippocampal neural cell proliferation under normal conditions and alcohol intoxication conditions has been recently studied; however, this effect under sensory stimulation application has not clarified yet. In the present study, the effect of compression stimulation application on hippocampal neural cell proliferation in the dentate gyrus in normal and alcohol intoxicated rats was investigated. Methods : Experimental design: comparative investigation on number of 5-Bromo-2'-deoxyuridine(BrdU)B-positive cells in dentate gyrus 5 days after commencement. Setting: animal laboratory. Participants: male Sprague-Dawley rats of 3weeks old in age weighing $80{\pm}10gm$. Intervention: animals were randomly assigned into 4 groups; control-rest group(n=8), control-compression group(n=8), alcohol intoxication-rest group(n=8) and alcohol intoxication-compression group(n=8). Animals of the alcohol intoxicated groups were injected intraperitoneally with alcohol(2g/kg) twice per day for 3 days. All animals were injected BrdU(50mg/kg) intraperitoneally, and rats compression stimulation application groups were compressed using sphygmomanometer cuff times per day, for 5 days following alcohol administration. Measures: mean number of BrdU-positive cells in dentate gyrus was observed via immunohistochemistry. Results : Compression stimulation application significantly increased the number of BrdU-positive cells in the dentate gyrus. Also, treatment with alcohol for 3 days inhibited cell proliferation, and compression stimulation application alleviated alcohol-induced inhibition of new cell formation. Conclusion : These results suggest the possibility that compression stimulation application may help in improvement following alcohol-induced brain damaged.

• 한국식품영양과학회지
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• 제22권4호
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• pp.398-404
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• 1993

화학적 발암제인 DMBA를 이용하여 챌스터 협낭에 암을 유발시키면서 마늘을 투입시켜 마늘의 유의성있는 항암 효과를 관찰하였다. 햄스터 헙낭에서 DMBA에 의한 발암현상에 대한 마늘의 효과를 병리조직학적으로 관찰한 결과 대조군에 비하여 마늘즙을 복용시킨 실험군에서 암세포의 침윤 증식상이 다소 감소되었다. 그리고 BrdU(bromodeoxy uridine)의 투여후 DMBA 발암현상에 대한 면역 조직 화학적 관찰에서 마늘을 복용시킨 실험군에 비하여 마늘즙을 복용시키지 않은 실험군에서 발암 개시전 기저 세포층의 증식성이 감소되어져 있으나 침윤 증가하는 발암 세포군에서는 서로 거의 유사한 증식상을 보였다. 이것으로 미루어 보아 마늘이 음식물에 적절하게 사용되고 조리하는 방법의 개발에 따라 일상 식생활에서 많이 사용하여 각종 암발생을 예방하는 일환이 되리라고 사료된다.

• Journal of Acupuncture Research
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• 제23권3호
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• pp.47-56
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• 2006

Methods : 40 of Female rats were allowed to mate with 40 of male rats. Then, female rats were delivered of offspring rats, After birth 28 days, offspring rats were divided 8 groups, The normal group(Group A), the 10 mg/kg Angelicae gig antis radix~treated group(Group B), the 50 mg/kg Angelicae gigantis radix-treated group(Group C), the 100 mg/kg Angelicae gig antis radix-treated group(Group D), The control (noise-treated) group(Group E). the noise -10 mg/kg Angelicae gigantis radix-treated group(Group F), the noise-50 mg/kg Angelicae gigantis radix~treated group(Group G), and the noise-100 mg/kg Angelicae gigantis radix-treated group(Group H)(n = 5 in each group), From the 15th day of pregnancy, all rats were subcutaneously injected with 50 mg/kg BrdU once a day 30 min before the starting of experimental treatment. Rats of the prenatal noise-treated group were applied with 95 decibel supersonic machine sound for 1h once a day until delivery, After birth 28 days, offspring rats intraperitoneally injected with 50 mg/kg of BrdU and offspring rats were treated Angelicae gigantis radix Herb-acupunture on chungwan(CV12) for 7 consecutive days. For the detection of BrdU-positive cells and Ki-67 positive cells in hippocampus, immunohistochemistry was performed. Results : 1. The number of BrdU-positive cells in the dentate gyrus of noise-treated group was significantly decreased to normal group, and the Group F, G, H were significantly increased to control group. 2. The number of Ki-67 positive cells in the dentate gyrus of noise-treated group was significantly decreased to control group, and the Group G, H were significantly increased to control group. Conclusion : We concluded that postnatal Angelicae gigantis radix administration has effect on cell proliferation in offspring rats with prenatal noise stress during pregnancy.