• Plant Resources
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• v.3 no.3
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• pp.223-225
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• 2000

A new forma, Cremastra appendiculta Makino f. albiflora B. Kil, f. nov. is described from Naejangsan, Jeollabuk-do, Korea. It is easily distinguished from C. appendiculata by having light white-green flower of outer perianth segment and white color of inner perianth segment of the flower.

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.3
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• pp.310-316
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• 2014

Oldenlandia diffusa, Cremastra appendiculata and Fritillaria thunbergii are widely distributed in the Korea, China and Japan, and has been used in traditional medicine for various diseases, such as pharyngolaryngitis, tonsillitis, goiter and stomach ulcer. However, the anti-cancer activities in human breast cancer have not been clearly elucidated yet. In this study, it was compared the in vitro cytotoxic effects of single and complex treatment of O. diffusa, C. appendiculata and F. thunbergii. We treat human breast cancer MCF-7 cells with O. diffusa, C. appendiculata and F. thunbergii. And we evaluated viability, growth inhibition, morphological changes, apoptotic bodies formation, measurement of the cell cycle and formation of DNA fragmentation of these cells. It was found that single treatment of O. diffusa could inhibit the cell proliferation in human breast cancer MCF-7 cells. However, complex treatment of O. diffusa, C. appendiculata and F. thunbergii is weakly or not affect the cell proliferation of MCF-7 cells. And anti-proliferative effects of O. diffusa in MCF-7 cells was associated with G1 arrest of cell cycle. These findings suggest that O. diffusa may be a potential chemotherapeutic agent for the control of human breast cancer cells and further studies will be needed to identify the molecular mechanisms.

• The Journal of Internal Korean Medicine
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• v.35 no.2
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• pp.133-144
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• 2014

O. diffusa, C. appendiculata and F. thunbergii are reported to possess many pharmacological activities including anti-oxidant, anti-inflammatory, anti-hypertension, anti-diabetic and anti-cancer effects. However, their anti-cancer activities in human breast cancer have not been clearly elucidated yet. Objectives: In the present study, we compared the in vitro cytotoxic effects of single and complex treatment of O. diffusa, C. appendiculata and F. thunbergii in human breast cancer MDA-MB-231 cells. Methods: After we treated human breast cancer MDA-MB-231 cells with O. diffusa, C. appendiculata and F. thunbergii. we evaluated viability, growth inhibition, morphological changes, apoptotic body formation, measurement of the cell cycle and formation of DNA fragmentation of these cells. Results: We found that single treatment of O. diffusa and F. thunbergii could inhibit cell proliferation in human breast cancer MDA-MB-231 cells. However, complex treatment of O. diffusa, C. appendiculata and F. thunbergii had weak or no effect on the cell proliferation of MDA-MB-231 cells. The first, anti-proliferative effects of O. diffusa in MDA-MB-231 cells was associated with G2/M arrest of cell cycle and apoptotic cell death. The second, anti-proliferative effect of F. thunbergii in MDA-MB-231 cells was associated with apoptotic cell death. Conclusions: Taken together, these findings suggest that O. diffusa and F. thunbergii may be a potential chemotherapeutic agent for the control of human breast cancer cells, further studies will be needed to identify the molecular mechanisms.

• Animal Systematics, Evolution and Diversity
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• v.16 no.2
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• pp.239-255
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• 2000

Seven freshwater naidid oligochaetes are recorded on the basis of the materials collected from four lowland swamps, Upo-neup, Oisong-neup, Chilnal-neup, and Chunam-ji, in Kyungsnagnam-do, Korea during the period from February 1988 to September 1991 : Chaetogaster diastrophus (Gruithuisen), C. diaphanus (Gruithuisen), Amphichaeta asiatica Liang, Pristina longiseta Ehrenberg, P. biserrata Chen, Stylaria fossularis Leidy, Slavina appendiculata (d'Udekem). These species are redescribed and illustrated with a key to the species and genera of the family Naididae in Korea. This is the first taxonomic record on the freshwater naidid oligochaetes in Korea.

• Journal of Species Research
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• v.4 no.2
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• pp.145-151
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• 2015

Morphological changes of flowers and insect visitors were observed to investigate pollinator of Caesalpinia decapetala. The flowers of C. decapetala are protandrous. Functionally, the flower is changed from male to female. As a male, pollen grain is released after anther dehisced while style is immature. After completed pollen grain release, the style starts to lengthen. It helps the stigma to easily touch the carpenter bee's thorax covered with pollen grain. At this time, flower functions as a female. The majority of taxa and individuals observed were Hymenoptera. The most frequent visitor was the Xylocopa appendiculata circumvolans, carpenter bee. Carpenter bees exhibited only typical pollinator behavior among flower visitors, with touching reproductive organs and seeking nectar at the same time. The pollination behavior is as follows. Soon after carpenter bees perceived guide mark, they foraged rightward and grasped style and stamens with legs and they inserted proboscis into standard petal to seek nectar. With this behavior, the pollen grains of the male flower transfer to the ventral thorax of the carpenter bee. As the carpenter bee moves to another female flower, the deposited pollen grains are delivered to the stigma.

• Korean Journal of Plant Taxonomy
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• v.49 no.3
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• pp.269-273
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• 2019

We report the meiotic chromosome numbers of four Carex taxa from Korean populations. Three are the first reports made on taxa from Korean populations: Carex appendiculata (Trautv. & C. A. Mey.) $K{\ddot{u}}k$. ($n=27_{II}$), C. fernaldiana H. $L{\acute{e}}v$. & Vaniot ($n=33_{II}$), and C. metallica H.$L{\acute{e}}v$. ($n=15_{II}$). Reports on the other species expand the range of variation in the chromosome number within a taxon, C. miyabei Franch. (n = $43_{II}$, $44_{II}$, $45_{II}$). Carex L. (Cyperaceae) consists of more than 2,000 species worldwide and is the most species-rich genus in Korea. The species diversity in the genus has been hypothesized to be associated with the chromosome variation, but chromosome information pertaining to Korean Carex taxa is not well known. This report updates the chromosome number inventory on Korean Carex to 24 out of 180 taxa.

• Journal of Species Research
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• v.9 no.2
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• pp.131-146
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• 2020

Siphonophores are unique, gelatinous zooplankton, which many individuals gather and live like one "Superorganism". The role of individuals in the colony differs greatly depending on their morphological difference, making them more unique. In this study, we report four species belonging to Diphyidae Quoy and Gaimard, 1827 sampled from the South Sea and off Jeju Island, Korea. Two Chelophyes Totton, 1932 (C. appendiculata (Eschscholtz, 1829); C. contorta (Lens and van Riemsdijk, 1908)) and two Eudoxoides Huxley, 1859 (E. mitra (Huxley, 1859); E. spiralis (Bigelow, 1911)) species are described with multi-focus stacked digital images. Our findings update the confirmed order Siphonophorae Eschscholtz, 1829 in Korea to be three suborders, five families, eight genera, and 13 species. In addition, we summarize the synonyms and global distributions of these four newly recorded species in Korean waters.

• Korean Journal of Pharmacognosy
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• v.25 no.3
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• pp.293-304
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• 1994

In order to estimate accurate originality of the important crude drugs, Gastrodia, Dendrobium, Bletilla and Cremastra spp. were investigated comparing their morphological, anatomical and physicochemical characteristics and antibacterial, antifungal activities. The results of the studies as follows: 1. In morphological and anatomical studies, Korean Gastrodia contained more mucilage duct and symbiotic fungi than imported Gastrodia. Korean Dendrobium contained starch grains but without starch grains in the imported Dendrobium from China enclosed with thicken silicified wall. The corm of Cremasta appendiculata contained raphides of bundles with mucilage while the bulbs of Tulipa edulis contained several various starch grains form. 2. In physiological and TLC analysis, crude drugs in Orchidaceae contained common constituents with fluorescence and much mucilage. 3. The EtOH extracts of Gastrodia, Dendrobium, Bletilla, Cremastra showed antibacterial activities against B. subtilis and E. coli (Dendrobium>Gastrodia>Bletilla>Cremastra). But, no antifungal activities against C. albicans, A. niger were observed.

• Korean Journal of Environment and Ecology
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• v.35 no.3
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• pp.237-255
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• 2021

Lychnis wilfordii (Regel) Maxim. is one of the wetland specialists mainly distributed in peatlands at high latitudes. In Korea, it is isolated in two regions. This study investigated habitats, growth traits, and self-compatibility of L. wilfordii and assessed the internal dynamics of its population persistence. Its population has remained stable in the Yongneup Wetland Protected Area (YWPA). There was a clear difference in vegetation environment between YWPA and the distribution area in Daegwallyeong (DWL), Pyeongchang-gun. It has self-compatibility while pollinators facilitate its seed production. It produces a large number of hibernacles and bears the maximum number of branches and fruits in soil with rich organic contents. However, it grows and bears fruits even under the condition of low organic contents. In YWPA, L. wilfordii is not distributed in high moor but widely distributed in low moor where tussocks by Carex thunbergii var. appendiculata are developed. It is mainly distributed on the top of tussocks also. Therefore, it is judged that the formation, growth, and extinction of tussocks by C. thunbergii var. appendiculata is closely related to the establishment, growth, and extinction of plants distributed in this space. It is assessed that the current YWPA has well-developed tussocks in which L. wilfordii is widely distributed, and extinction and re-establishment progress well. Accordingly, the L. wilfordii population is expected to be sustainable in the long term given if its current ecological process is maintained well.

• Korean Journal of Environment and Ecology
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• v.36 no.1
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• pp.1-29
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• 2022

This study surveyed the Mt. Teomo·Hyeolgu and surrounding areas in Ganghwa Island, located in Ganghwa-gun, Incheon, to identify vascular flora and alien plants to be used as basic data for systematic management of the remarkable plants and biodiversity conservation. The survey was carried out 13 times from March 2019 to October 2020. The identified vascular flora of Mt.Teomo·Hyeolgu was 101 families, 321 genera, 517 species, 13 subspecies, 38 varieties, 5 forms, and 573 taxa. They accounted for about 12.35% of a total of 4,641 taxa of vascular plants in Korea. The identified Northern lineage plants, endemic plants, and rare plants on the Korean Peninsula as designated by IUCN were 68 taxa, 10 taxa, and 9 taxa, respectively. 69 taxa were classified as floristic target species. Three taxa, including Maui sedge [Carex thunbergii var. appendiculata (Trautv. & C.A.Mey.) Ohwi] were identified s Class IV and 14 taxa, including Violet Stanavoi clematis (Clematis fusca var. violacea Maxim.) were identified as Class III. The naturalized plants that appeared in the study site were 63 taxa, including daisy fleabane [Erigeron annuus(L.) Pers.], common ragweed [Ambrosia artemisiifoliaL.] and Canadian fleabane [Conyza canadensis(L.) Cronquist]. The above three species were found to have spread throughout Ganghwa Island and surrounding areas such as Seokmo Island, Gyodong Island, and Gimpo city. As introduced disturbing plants like prickly lettuce [Lactuca scariola L.], bur cucumber [Sicyos angulatus L.] and field dock [Rumex acetosella L.] have been newly reported in the Mt. Teomo·Hyeolgu area, mid- to long-term conservation measures should be established for native plants, such as remarkable plants, and also management measures like physical removal should be prepared at an early stage.