• Title/Summary/Keyword: CFA

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Gastrointestinal Carriage of Escherichia coli with Hemagglutination Activity and Colonization Factor Antigen I and its Relation to Diarrhea (Mannose-resistant Hemagglutination(MRHA) 및 Colonization Factor Antigen I(CFA I)을 표현하는 대장균의 장관내 존재와 설사증 발현과의 관계)

  • Ro, Sung-Hyun;Kim, Kyung-Hee;Cho, Yaug-Ja;Suh, Inn-Soo
    • The Journal of the Korean Society for Microbiology
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    • v.22 no.2
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    • pp.147-153
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    • 1987
  • Colonization factor antigen I(CFA I) has been shown to be one of several virulence factors that promote attachment of enterotoxigenic E. coli(ETEC) to small intestinal epithelial cells of humans. The ability of ETEC to produce mannose-resistant hemagglutination(MRHA) of human blood group A has been used to detect CFA I. To determine gastrointestinal carriage in Korean children of E. coli with MRHA and CFA I, 116 strains of E. coli from diarrheal children admitted to Hanyang University Hospital were examined for MRHA of human erythrocytes and the presence of CFA I. Of 45 ETEC strains, 18(40%) gave a positive MRHA($MRHA^+$) and eight(18%) were positive for CFA I(CFA $I^+$). ETEC with CFA I were all heat-stable enterotoxin(ST) producers and two of these strains were of serogroups $O_{25}$. Of 17 classic enteropathogenic E. coli(EPEC), 7(41%) were $MRHA^+$ but all were negative for CFA I(CFA $I^-$). Of 30 enteroadherent E. coli(EAEC) strains, 11(37%) were $MRHA^+$ and one was CFA $I^+$. Of 24 nonpathogenic E. coli, 4(17%) were $MRHA^+$ but all were CFA $I^-$. It was shown that MRHA was common in all strains of E. coli, CFA I was limited only to ST producing ETEC and EAEC; although MRHA is a useful screening procedure, serologic tests seem to be necessary to comfirm CFA I production. CFA I was associated with a lower proportion of ETEC isolates in Korea than has been reported for other locations.

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Evaluation System for Color Filter Array (CFA) in Digital Camera (디지털 카메라에서 컬러 필터 어레이를 위한 평가 시스템)

  • Bae, Tae Wuk
    • Journal of Korea Multimedia Society
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    • v.20 no.11
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    • pp.1741-1749
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    • 2017
  • In commercial digital-cameras, color-filter filters light according to wavelength range of color filter array (CFA) and the filtered intensities contain color information of light. Then, output data of CFA is transformed to final rendered image through demosaicing process. In image processing of digital-camera, the quality of the final rendered image is affected by optical cross talk of CFA, kind of CFA pattern etc. Basically, pattern of CFA plays important role in image quality of final image rendered by digital-camera. Therefore, an evaluation system capable of quantitatively evaluating CFA is needed. This paper proposes a novel evaluation system using existing and proposed image metrics for evaluating CFAs of digital-camera. Proposed CFA evaluation system consist of color difference in CIELAB and S-CIELAB, Structure SImilarity (SSIM), MTF50, moire starting point (MSP), and subjective preference (SP). MSP and SP are newly designed for the proposed evaluation system. Proposed evaluation system is expressed in polar coordinates to analyze the characteristics of CFA objectively and intuitively. Through simulations, we confirmed that proposed CFA evaluation system can objectively assess performance of developed CFAs.

Antigenicity Study of CFA-001, cefazolin, a cephalosperin Derivative Produced by an :Enzymatic Semisynthesis

  • Park, Jong-Il;Jeong, Tae-Cheon;Kim, Hyoung-Chin;Han, Sang-Seop;Roh, Jung-Koo;Kim, Jeong-Hwan;Jeon, Yeong-Joong;Kim, Dal-Hyun;Kim, Je-Hak;Park, Kwan-Ha
    • Biomolecules & Therapeutics
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    • v.5 no.1
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    • pp.48-52
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    • 1997
  • The antigenic potential of CFA-001, cefazolin, a cephalosporin derivative produced by an enzy-matic semisynthesis, was determined in Hartley guinea pigs. A battery of tests employed consisted of active systemic anaphylaxis (ASA), passive cutaneous anaphylaxis (PCA), and indirect hemagglutination test (IHA). The results were as follows: 1) In ASA, no signs attributable to anaphylaxis was observed in guinea pigs sensitized with CFA-001, whereas OVA-sensitized animals induced severe anaphylactic symptoms; 2) guinea pigs did not produce antibodies against CFA-001 when sensitized with or without Freund's complete adjuvant (FCA) in homologous PCA tests. Meanwhile, antibodies against ovalbumin (OVA) were clearly detected; 3) No CFA-001-specific hemagglutination was observed in the IHA using sera obtained from CFA-001- sensitized guinea pigs. These results suggest that CFA-001 has no antigenicity potential in guinea pigs.

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Effects of Coal Fly Ash as a Bulking Agent under Co-composting with Swine Manure and Saw Dust

  • Lee, Chang Hoon;Park, Seong Jin;Kim, Myung Sook;Yun, Sun Kang;Sonn, Yeon Kyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.47 no.5
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    • pp.356-361
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    • 2014
  • The coal fly ash (CFA) may be utilized as an extender for organic waste composting at the same time fully expected to solve all industrial waste disposal and sawdust tribe. The main objective in this study was to evaluate the effect of CFA addition as a bulking agent for swine manure composting. To determine the suitable addition rate of CFA as a bulking agent, 0, 10, 20 and 30% of saw dust were mixed with 30, 20, 10 and 0% of coal fly ash, respectively. Compost quality for swine manure composting was to evaluate temperature, pH, C/N ratio, and phytotoxicity as germination index. Stability of compost increased with increasing levels of CFA as bulking agent during swine manure composting due to the high alkaline materials including CFA. C to N ratio in treatment added CFA was higher than that of the control without CFA. After finishing composting, germination index of lettuce and cabbage in swine manure compost added 10% of CFA was similar to the control, all the heavy metal contents were far below the stipulated standard for organic farming. These results indicated tahr coal fly ash as bulking agents might be alternative materials to save saw dust and apply industrial products for swine manure composting.

A Study on Resonance and Interference of a Cooling Fan Assembly by Using FEM (유한요소법을 이용한 쿨링팬의 진동 및 간섭에 관한 연구)

  • 정일호;송하종;박태원;김주용
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2004.05a
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    • pp.919-924
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    • 2004
  • A CFA(Cooling Fan Assembly) is composed of a fan, motor and shroud, which is at the back of the automotive radiator. By forcing the wind to pass, the CFA controls the cooling performance of the radiator. The noise and vibration of the CFA may be primarily due to the resonance between the CFA and engine. The Interference among the fan, shroud and radiator by deformation is considered when the CFA is designed. In this paper, in order to analyze the structural vibration of the CFA for automobiles, a finite element model of the CFA is established by using a commercial FEM code. After the finite element modeling, the natural frequencies and the mode shapes are obtained from the FE analysis. The natural frequencies are obtained from the vibration test as well. Then, the results of the vibration test are compared with those of the FE analysis. The natural frequencies obtained by experiment have a great similarity to the results from FE model. We have confirmed the validity of the FE model and verify the structural safety for the resonance. The stress and displacements are obtained from FE analysis. We have confirmed the safety for the interference and failure.

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The Effects of Warm Needling at GB30·GB34 on Complete Freund's Adjuvant(CFA)-induced Rheumatoid Arthritis in Rats (환도(環跳)(GB30)·양릉천(陽陵泉)(GB34) 온침(溫鍼)이 CFA로 유발된 백서의 류마티스 관절염에 미치는 영향)

  • Jeong, Joo Yong;Cho, Myoung Rae
    • Journal of Acupuncture Research
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    • v.32 no.2
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    • pp.65-75
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    • 2015
  • Objectives : The purpose of this study was to investigate the effects of warm needling at $GB_{30}{\cdot}GB_{34}$ on Complete Freund's Adjuvant(CFA)-induced rheumatoid arthritis in rats. Methods : Arthritis was induced by injecting CFA subcutaneously into the left knee joint and paw. Acupuncture(AT) and warm needling(W-AT0.5, W-AT1.0) were injected at $GB_{30}{\cdot}GB_{34}$, every other day for a total of 5 times beginning on day 10 after the CFA injection. Thereafter, external shape, paw edema, serum aminotransferase and anti-inflammatory factors were assessed, and hematological and histological observations were made. Results : In paw edema volume all 3 groups(AT, W-AT0.5, W-AT1.0) showed significant decrease compared to the CFA control group. In TNF-${\alpha}$ and IL-6, all 3 groups showed significant decrease compared to CFA control group. In AST and ALT all 3 groups showed no significant change. In IL-$1{\beta}$, W-AT0.5 and W-AT1.0, groups showed significant decrease compared to the CFA control group. Leucocyte, erythrocyte and thrombocyte, all 3 groups showed no significant change. In histological observations, all 3 groups were similar to the intact group in terms of synoviocyte, cartilage lacuna and cartilage cells. Conclusions : The results suggest that warm needling at $GB_{30}{\cdot}GB_{34}$, has the effect of suppressing inflammation of CFA-induced rheumatoid arthritis in rats.

Effect of $1{\alpha},\;25\;Dihydroxyvitamin\;D_3$ on Osteoporosis in Rats ($1{\alpha},\;25\;Dihydroxyvitamin\;D_3$이 랫드 골다공증에 미치는 영향)

  • Bae, Chun-Sik;Choi, Seok-Hwa
    • Applied Microscopy
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    • v.32 no.3
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    • pp.223-230
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    • 2002
  • Vitamin D is one of important factors involved in the regulation of bone metabolism. In osteoporosis, the therapeutic effect of vitamin D on the healing process has still been controversial. To confirm the effects of $1{\alpha},\;25\;Dihydroxyvitamin\;D_3$ on the osteoporosis, the change of bone mineral density and the histologic changes of osteoporotic femur were examined comparatively in normal control group (positive control), CFA control group (negative control), CFA+$1{\alpha}$, 25 dihydroxyvitamin $D_{3}\;0.01{\mu}g/kg$ group (Vit $D_{3}L$) and CFA+$1{\alpha}$, 25 dihydroxyvitamin $D_{3}\;0.1{\mu}g/kg$ group (Vit $D_{3}H$) after osteoporosis was induced by single injection of complete Freund's adjuvant (CFA) in rats. The value of bone mineral density and bone mineral content of femur was increased in both Vit $D_{3}L$ and Vit $D_{3}H$ than CFA control, and the increase rate of that was higher in Vit $D_{3}H$ than Vit $D_{3}L$. In CFA control, the size of the bone marrow cavities significantly increased and the width from the bone marrow cavity and cortex significantly decreased than normal control. In Vit $D_{3}H$ and Vit $D_{3}L$, the increase rate of the size of bone marrow cavity and the decrease rate of the width from the bone marrow cavity and cortex was depressed than CFA control. These results suggest that $1{\alpha},\;25\;Dihydroxyvitamin\;D_3$ has therapeutic effect on adjuvant-induced osteoporosis in rats.

A Pseudo Multiple Capture CMOS Image Sensor with RWB Color Filter Array

  • Park, Ju-Seop;Choe, Kun-Il;Cheon, Ji-Min;Han, Gun-Hee
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.6 no.4
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    • pp.270-274
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    • 2006
  • A color filter array (CFA) helps a single electrical image sensor to recognize color images. The Red-Green-Blue (RGB) Bayer CFA is commonly used, but the amount of the light which arrives at the photodiode is attenuated with this CFA. Red-White-Blue (RWB) CFA increases the amount of the light which arrives at photodiode by using White (W) pixels instead of Green (G) pixels. However, white pixels are saturated earlier than red and blue pixels. The pseudo multiple capture scheme and the corresponding RWB CFA were proposed to overcome the early saturation problem of W pixels. The prototype CMOS image sensor (CIS) was fabricated with $0.35-{\mu}m$ CMOS process. The proposed CIS solves the early saturation problem of W pixels and increases the dynamic range.

Effect of Electroacupuncture on AMPA Receptor GluR2 Subunit in Complete Freund's Adjuvant-induced Inflammatory Pain Model

  • Kim, Chul-Yun;Choi, Hye-Young;Yang, Yeun-Jin;Choi, Byung-Tae
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.5
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    • pp.881-886
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    • 2011
  • AMPA receptor (AMPAR)s are heterotetrameric structures made up from 4 units (GluR1-4) and are thought to underlie perception of persistent inflammatory pain. Complete Freund's adjuvant (CFA)-evoked inflammation induces synaptic GluR2 internalization, which is initiated by GluR2 phosphorylation, in dorsal horn neurons during the maintenance of CFA-induced hypersensitivity. The present study investigated whether electroacupuncture (EA) stimulation has any effect on GluR2 trafficking by using immunoblot and immunohistochemistry. We examined that CFA-induced dorsal horn GluR2 internalization was attenuated by EA treatment. EA treatment could also decrease the level of pGluR2 regardless of whether CFA injection was administrated or not. In addition, previous studies suggest that microglial cells are increased without morphological change in CFA injected animal. In our study, increases in microglial cells in CFA group were observed, whereas EA with or without CFA-injected group showed similar aspects with normal group. In conclusion, our results indicate that EA might blunt CFA-evoked inflammation by coordinating mechanisms at the upstream step of neuron activation and GluR2 phosphorylation.

Continuous-Flow Analysis for Determination of Nitrate Using Hydrazine-Copper Method in Plan (Hydrazine-Copper 방법을 이용한 연속흐름제어장치를 통한 식물체의 nitrate 분석)

  • Park, Yang-Ho;Park, So-Hyeon;Lee, Ju-Young;Jang, Byoung-Choon;Lee, Ki-Sang
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.4
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    • pp.204-208
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    • 2006
  • This study is to describe continuous-flow analysis (CFA) for the determination of nitrate using hydrazine-copper in plant material and to test precision of this method compared with that of methods, which are RQflex method and salicylic acid method. Samples were leaves of watermelon, cucumber, melon and tomato. Nitrate values measured by the RQflex method were greater than those measured by CFA or salicylic acid method. The correlation of nitrate values between those measured by CFA and salicylic acid method was $R^2=0.9671$, and those measured by CFA between those measured by RQflex method was $R^2=0.9739$. Recovery rate of nitrate added to tissue extract by CFA method was $99.7{\pm}0.25%$.