• Journal of Microbiology and Biotechnology
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• v.22 no.12
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• pp.1644-1652
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• 2012

Iron plays a key role in host-pathogen interactions. Microbial pathogens require iron for survival and virulence, whereas mammalian hosts sequester and withhold iron as a means of nutritional immunity. We previously identified two paralogous genes, CFT1 and CFT2, which encode homologs of a fungal iron permease, Cft1 and Cft2, respectively, in the human fungal pathogen Cryptococcus neoformans. Cft1 was shown to play a role in the high-affinity reductive iron uptake system, and was required for transferrin utilization and full virulence in mammalian hosts. However, no role of Cft2 has been suggested yet. Here, we identified the third gene, CFT3, that produces an additional fungal iron permease homolog in C. neoformans, and we also generated the cft3 mutant for functional characterization. We aimed to reveal distinct functions of Cft1, Cft2 and Cft3 by analyzing phenotypes of the mutants lacking CFT1, CFT2 and CFT3, respectively. The endogenous promoter of CFT1, CFT2 and CFT3 was replaced with the inducible GAL7 promoter in the wild-type strain or in the cft1 mutant for gain-of-function analysis. Using these strains, we were able to find that CFT2 is required for growth in low-iron conditions in the absence of CFT1 and that overexpression of CFT2 compensates for deficiency of the cft1 mutant in iron uptake and various cellular stress conditions. However, unlike CFT2, no clear phenotypic characteristic of the cft3 mutant and the strain overexpressing CFT3 was observed. Overall, our data suggested a redundant role of Cft2 in the high-affinity iron uptake and stress responses in C. neoformans.

• Journal of the Earthquake Engineering Society of Korea
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• v.12 no.3
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• pp.29-36
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• 2008

The TR-CFT(Transversely Reinforced Concrete Filled Steel Tube) column is proposed to control or at least delay the state of local buckling at the critical section by wrapping the CFT columns with a carbon fiber sheet. In this study, an equation to determine the flexural strength of TR-CFT is proposed. The ACI-318 code, in which the contribution of the confining effect in the concrete filled steel tube is not appropriately accounted for, may be conservative. Therefore, flexural strength design equations for CFT columns and TR-CFT columns are proposed based on the concrete strain-stress curve, which contributes to the confining effect. Finally, the predicted results for the CFT and TR-CFT columns are shown to be in good agreement with actual test results.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.28 no.5
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• pp.503-510
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• 2015

In this paper, the blast resisting performance of partially reinforced CFT columns was compared with the normal CFT columns to evaluate the effect of reinforcing with steel plates. Autodyn which is a specialized hydro-code for analysis of explosion and impact was used to simulate the structural behavior of the CFT columns under the blast loadings. The interaction between concrete and surrounding steel plates was modeled with friction and join option to represent the realistic damage of columns. According to the analysis, the partially reinforced CFT column showed enhanced blast resisting performance than the normal CFT columns. Also the improvement of blast resisting performance was depended on the height of reinforcing steel plates.

• Journal of Korean Society of Steel Construction
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• v.19 no.4
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• pp.403-411
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• 2007

CFT (Concrete filled steel tube) column has become popular for building construction due to not only its composite effect but also economic effect. However, the conventional CFT column also has its own disadvantages having plastic buckling at the end of column followed by the reduction of strength by yielding of steel tube. An experiment on TR-CFT (Transversely reinforced CFT) column are conducted for making up for conventional CFT column's disadvantages. The experiment parameters are strength of concrete, the layer numbers of carbon fiber sheet. In this study, hysteretic curve, initial stiffness, strength, plastic deformation capacity, and dissipated energy are compared and analyzed between CFT and TR-CFT columns.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.4A
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• pp.315-327
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• 2009

In this study, the static test of CFT truss girders for different f/L ratios was conducted to determine how the ultimate strength of the CFT truss girder was affected by different f/L ratios. A total of two CFT truss girders were constructed and tested under bending condition. The length of all specimens is 20,000 mm. The CFT truss girder is a tubular truss composed of chord members made of concrete-filled circular tubes. The main parameter analyzed in the experimental study was the f/L ratio. This factor was experimentally investigated to assess their influence on ultimate strength and stiffness. The test results show that CFT truss girder has good elastic-plastic property and ductility. The presence of the f/L ratios in CFT truss girders alters its ultimate strength because of the global stiffness of the CFT truss girders. The ultimate strength of CFT truss girders increases as the f/L ratio increases. If the f/L ratio of the CFT truss girders increases twofold, the ultimate strengths increase by 80%. The CFT truss girders showed that they retained large deformation capacity, even after reaching the ultimate strength. Results of this investigation demonstrated the potential for efficiently using a CFT truss as a bridge girder.

• Journal of the Korea Concrete Institute
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• v.13 no.2
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• pp.93-98
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• 2001

CFT column has excellent structural properties due to the composite action between concrete and steel tube. The bond behavior between the constituent elements has to be found for analyzing the behavior of CFT column. A new model is necessary because most of existing models for bond stress-slip relationship of the deformed bar cannot be applied to the CFT column. Therefore, the objective of this research is to develop a new model related to the bond behavior of CFT column considering the relation between bond stress and vertical stress, and the distribution of lateral stress under the confinement created by steel casing. From equilibrium condition, the formula for relationship between bond stress and vertical stress is derived, and the relationship for the lateral stresses of the CFT column section is obtained by an Airy stress function. The experiments are performed for five CFT column specimens axially loading on concrete alone. The relation between bond strength and lateral stress is investigated from the regression analysis using the measured strains. Finally a new bond strength model is proposed, which is able to predict the relationship for the stress of each direction of CFT column loading on concrete.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.2A
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• pp.149-159
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• 2010

This paper presents an experimental study on the structural behavior of composite CFT truss girder bridge with full depth precast panels. The length of span is 20,000 mm. The CFT truss girder is a tubular truss composed of chord members made of concrete-filled and hollow circular tubes. To determine fundamental structural characteristics such as the strength and deformation properties of composite CFT truss girder bridge, static and dynamic tests were conducted. The natural frequencies calculated by the FEM are in good agreement with experimental results obtained from dynamic test. Bracing have only a small effect on the natural frequencies of composite CFT truss girder bridge as indicated by the FEM results. The yield strength and deformation of the composite CFT truss girder bridges were investigated through a static bending test. Besides, the test results showed that uniform distribution of shear connectors can be applicable in composite CFT truss girder bridges.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.1A
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• pp.19-30
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• 2009

The primary objective of the present study was to attempt to quantify the effect of the existing codes for CFT composite section on initial section flexural stiffness, based on the measured vibration frequency of CFT truss girders. The formulae for the initial flexural stiffness of the composite sections in the different codes are compared with the free vibration test results. The results of the free vibration test on the CFT truss girders are in good agreement with the analysis results when used in ACI formulae. The free vibration analysis of CFT truss girders for different f/L ratios was conducted to determine how the natural frequency of the CFT truss girder is affected by different f/L ratios. The presence of the f/L ratios in CFT truss girders alters its frequencies of vibration because of the global stiffness of the CFT girders. The frequency in horizontal modes decreases as the f/L ratio increases. However, the frequency in vertical modes increases as the f/L ratio increases.

• Fire Science and Engineering
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• v.31 no.2
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• pp.9-16
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• 2017

In this study, fire resistance tests were conducted to evaluate the fire resistance performance of unprotected and non-welded CFT columns in relation to the shape and size of cross-sections. Unprotected slot-type CFT columns which were ${\square}300$ and ${\square}500$ in dimensions resisted fire for 125 minutes and more than 180 minutes, respectively. Strain analysis showed that slot-type CFT columns were more ductile than welded CFT columns. The temperatures of central parts measured when welded CFT columns and slot-type CFT columns had lost fire resistance performance were higher in the former than the latter. Therefore, slot connection does not a great influence on the temperatures inside the concrete.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.3A
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• pp.139-148
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• 2012

Circular concrete-filled tubes (CFTs) are composite members, which consists of a steel tube and concrete infill. CFTs have been used as building columns and bridge piers due to several advantages such as their strength-to-size efficiency and facilitation of rapid construction. Extensive experimental studies about CFT have been conducted for past decades. However experimental results alone are not sufficient to support the engineering of these components. Complementary advanced numerical models are needed to simulate the behavior of CFT to extend the experimental research and develop predictive tools required for design and evaluation of structural systems. In this study, a finite element modeling technique for CFT was developed. The confinement effects, and behavior of CFT subjected various types of loading predicted by the proposed finite element model for CFT were verified by comparing with test results.