• Asian-Australasian Journal of Animal Sciences
• /
• v.19 no.6
• /
• pp.806-810
• /
• 2006

Candida utilis can assimilate fatty acids, so it was hypothesized that the treatment of rice by Candida utilis would improve feed quality by reducing fat content and adding the yeast function that would stimulate rumen microbes. In this study, the oil assimilation ability of Candida utilis IFO1086, 0988, 0626 and the effect of treatment of Candida utilis IFO1086, IFO0626 on the nutrient contents of rice bran were examined. The effect of Candida utilis addition on the in vitro degradability of forage was also investigated. It was found that the oil assimilating ability of IFO1086 and IFO0626 was significantly (p<0.01) higher than that of IFO0988. Candida utilis treatment reduced the EE content and increased the CP, ADF and NDF percentage. The absolute amount of ether extract was decreased by 35.9% in IFO1086 and IFO0626 treatment. The absolute amount of crude protein was not changed by yeast treatment. The ADF and NDF amounts were increased. The addition of Candida utilis increased in vitro forage degradability significantly (p<0.05). Based on these results it can be postulated that treatment of rice bran by Candida utilis may improve feed quality by reducing fat content, increasing the CP content and adding the function of yeast for stimulating rumen microbes.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.24 no.3
• /
• pp.434-439
• /
• 1995

Chinese radish juice(CRJ) was used as a culture medium for the production of Candida utilis ATCC 42416 biomass. Soluble solid and total sugar contents of Chinese radishes were in the range between 5.5 and $8.8^{\circ}$Brix and 3.5 and 6.5%, respectively. Since sugar in radishes are in readily usable forms, pretreatm ent procedures were not necessary after the extraction of juice from fresh radishes. In shake flask experimetns, C. utilis ATCC 42416 grew well in CRJ and completed growth in 24 hrs at $30^{\circ}C$ and 200 rpm. Maximum cell dry weight obtainable from a liter of CRJ(1.0% sugar $DCRJ{\times}5$) was 21.5g, when the yeast was grown on CRJ diluted 5 times or more with tap water to make sugar content to be eual to or less than 1.0%. Supplementation of 5-fold diluted CRJ with some nutrients did not greatly influence the growth rate, yeast biomass production, or cell protein content significantly, indicating that CRJ itself was a good substrate for the production of biomass by C. utilis ATCC 42416.

• Korean Journal of Food Science and Technology
• /
• v.20 no.1
• /
• pp.90-94
• /
• 1988

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunodiffusion method were used for the species differentiation of yeasts, Saccharomyces cerevisiae, Candida utils, Candida tropicalis, and Kleuyveromyces fragilis. Comparing the electrophoretic patterns of soluble and membrane proteins, Saccharomyces cereνisiae was similar to Candida utilis but was different from Candida tropicalis and Kleuyveromyces fragilis. In immunochemical properties of soluble proteins, Saccharomyces cerevisiae was almost identical with Candido utilis. However, Saccharomyces cerevisiae or Candida utilis was quite different from Candida tropicalis and Kleuyveromyces fragilis in their immunoreactivities. In immunochemical properties of membrane proteins, almost the same results were obtained irrespective of four yeast species. By using SDS-PAGE and immunodiffusion methods, Saccharomyces cerevisiae and Candida utilis were difficult to differentiate but both species were easily differentiated from Candida tropicalis and Kleuyveromyces fragilis.

• Fisheries and Aquatic Sciences
• /
• v.8 no.4
• /
• pp.235-242
• /
• 2005

Baker's yeast, Saccharomyces cerevisiae, has been widely used as a food organism for rotifers used in the larval production of marine fish. However, the nutritional value of the yeast is relatively poor compared with that of the marine alga Chlorella. We examined the nutritional value of another yeast, Candida utilis, and whether its food value could be increased through manipulation such as a cell wall treatment. Candida utilis and S. cerevisiae and their manipulated varieties were assessed with regard to the growth and nutrition of the rotifer Brachianus plicatilis. Larvae of the flounder Paralichthys alivaceus were cultured with rotifers fed on the yeast species, and the dietary value of the rotifers for the larvae was examined. Rotifers that were fed C. utilis grew faster than those provided with S. cerevisiae. Rotifers grew slightly faster on manipulated yeast than on non-manipulated yeast varieties. Of the two yeast species, C. utilis had better dietary value for rotifers. Flounder larvae cultured with rotifers that had fed on C. utilis displayed better growth and survival ($\%$) than did those cultured with rotifers that had fed on S. cerevisiae. Although the manipulated variety of C. utilis was better than the non-manipulated variety in terms of rotifer growth, the flounder larvae survived ($\%$) and grew better when they were fed rotifers that had eaten non-manipulated C. utilis. However, the nutritional value of this yeast species was still lower than that of Chlorella.

• Korean Journal of Food Science and Technology
• /
• v.13 no.2
• /
• pp.91-100
• /
• 1981

As a primary study for SCP production from soybean curd waste water, selection of yeast and optimum cultivation condition of selected yeast on soybean curd waste water were investigated. Eighteen strains of the genus Candida and Saccharamyces were tested to compare their abilities to grow on soybean curd waste water. Candida utilis YUFE 1508 and Candida guilliermondii KFCC 35120 grew most successfully. Optimum pH and optimum temperature of the basal medium for growth of the two strains were $6.0{\sim}6.5$ and $25^{\circ}C$, respectively. The optimum culture medium of the two yeasts was soybean curd waste water supplemented with molasses 2.5% (as total sugar), ammonium acetate 0.1-0.3% (as nitrogen), $KH_2PO_4$ 0.1-0.2% (as phosphorus), and $K_2HPO_4$ 0.05% (as phosphorus). But yeast growth was not affected by metal salts. Under the optimum cultivation condition, the maximum cell weights of Candida utilis YUFE 1508 and Candida guillfermondii KFCC 35120 were 1.313g and 1.322g/100ml of culture broth respectively after 48 hr of cultivation. The cell yields of Candida utilis YUFE 1508 and Candida guilliermondii KFCC 35120 were 68.4% and 74.2%, respectively, based on utilized sugar. On the other hand, crude protein of dry yeast produced by Candida utilis YUFE 1508 and Candida guilliermondii KFCC 35120 under optimum condition was 54.0% and 56.8%, respectively.

• Korean Journal of Food Science and Technology
• /
• v.24 no.3
• /
• pp.221-225
• /
• 1992

The possibility of using Chinese cabbage juice as a substrate for the production of Candida utilis cell mass was explored. Dry cell weight production and cell yield coefficient were 1.35-1.45 g/100 ml undiluted juice and 47-50%, respectively, when C. utilis was grown by shake flask culture at $30^{\circ}C$ for 24 hr on more than three-fold diluted Chinese cabbage juice to make the final sugar content be equal to or less than 1.0%. Supplementation of glucose(2%), $KH_2PO_4(0.2%)$ and $(NH_4)_2SO_4(0.2%)$ to three-fold diluted Chinese cabbage juice did not enhance the dry cell weight yield or the protein content of the yeast cell, while supplementation of yeast extract(0.2%) and peptone(0.2%) increased dry cell weight production and protein content but not as much as the amount of each nutrient added. It was found that Chinese cabbage juice was an excellent substrate for the cultivation of C. utilis.

• Journal of the East Asian Society of Dietary Life
• /
• v.5 no.3
• /
• pp.233-238
• /
• 1995

CDP-choline is known as an intermediate of lecithin biosynthesis, and as an important drug for nervous diseases of the brain, For the bioconversion of CMP and choline to CDP-choline, ATP is required as an energy source. In these studies, the biosynthetic reaction of CDP-choline was coupled with ATP regenerating system by glycolysis. As a microorganism containing the highest conversion activity of CMP and choline to CDP-choline, Candida utilis ATCC 42416 was selected. The optimum reaction condition were 50mM choline chloride, 20mM CMP, 100mM potassium phosphate (pH8.0), 300mM glucose, 50mM MgSO4, 10% dried cells with shaking incubation at 3$0^{\circ}C$. The reaction was thus performed for 10 hours under the above optimum conditions. The concentration of CDP-choline was 16mM(80% in conversion ratio).

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.33 no.1
• /
• pp.20-24
• /
• 2000

A 7-week growth trial was conducted to investigate the effects of yeasts (Kluyveromyces fragilis and Candida utilis) with or without cell wall chemical treatment (protoplasted) in formulated diets on growth and body composition of larval ayu (Plecoglossus altivelis). Three replicate groups of ap average weighing 100 mg were fed diets containing each level ($5{\%}$) of K. fragilis, protoplasted K. fragilis, C. utilis, protoplasted C. utilis or brewer's yeast as an additive. Survival rate of fish fed the diet containing protoplasted K. fragilis, C. utilis or protoplasted C. utilis was higher than that of fish tea the control diet (P<0.05). Body weight .gain of fish fed the diet containing protoplasted K. fragilis was higher than that of fish fed the control diet (P<0.05). Crude protein and ash contents of Ssh were not significantly affected by the different dietary yeasts (P>0.05), On the other hand, crude lipid content of fish fed the diet containing K. fragilis, protoplasted K. fragilis or brewer's yeast was higher than that of fish fed the control diet (P<0.05). Amino acids composition of fish was not significantly affected by the different dietary yeasts (P>0.05), except aspartic acid. The results suggest that protoplasted K. fragilis as an additive in micro-formulated diet can improve weight gain and body quality of larval ayu.

• Microbiology and Biotechnology Letters
• /
• v.11 no.3
• /
• pp.175-180
• /
• 1983

Thialysine significantly inhibited the growth of wild type strain Gondida utilis NCYC-359. In the absence of thialysine, the culture reached stationary phase after 24hr, however, in the presence of 0.5% thialysine, the culture reached stationary phase after 40hr, respectively. Effect of amino acid or vitamin was investigated on recovery of the growth of wild type strain from thialysine inhibition. Glycine, methionine, arginine and tryptophan recovered growth inhibition by thialyslne to some extent. However, vitamins were inert. Especially, lysine at one eighth concentration of thialysine recovered almost fully the growth inhibition. Thialysine resistant mutants were induced from the parent strain of Condida utilis NCYC-359 by NTG treatment. Colonies of thialysine resistant mutants were obtained on agar minimal medium supplemented with 0.1-0.5% thialysine. The frequency of thialysine resistant mutants induced by the first mutation was the highest at 0.1% The wild strain produced no appreciable lysine extracellularly. However, almost thialysine resistant mutants excreted appreciably. Lysine excretion increased after repeated mutation. Finally, of the thialysine resistant mutants induced by NTG, Condida utilis TRN-4006 was obtained. This strain excreted lysine (400$\mu\textrm{g}$/$m\ell$) into the medium with a concomitant decrease of lysine in the intracellular pool.

• Proceedings of the KAIS Fall Conference
• /
• 2004.06a
• /
• pp.307-310
• /
• 2004

본 연구에서는 분리대두단백(ISP: isolated soy protein)과 탈지대두박(DSF: defatted soy flake)을 Devolase Flavourzyme 효소로 가수분해한 각각의 HVP를 발효 배지로 사용하여 Candida utilis(KCCM 50342) 효모의 ethylalcohol, 4-ethylguaiacol의 생성과 향 생성의 주요 인자인 a -galactosidase의 활성을 측정하였다. Ethylalcohol은 발효 1일째에 탈지대두박을 원료로 하고 여과후 미생물 처리한 경우에서 가장 높게 생성되었고, 4-ethylguaiacol은 일부 여과하지 않고 미생물 처리한 경우에서 낮은 농도의 생성을 보였다. a-galactosidase 효소활성은 탈지대두박 보다 ISP를 원료로 한 HVP에서 높은 활성을 보였다. 관능검사결과 탈지대두박을 사용한 HVP의 관능적 특성이 ISP를 원료로 한 HVP보다 더 우수하였다. 결과적으로 탈지대두박을 원료로 한 HVP의 향기성분 및 관능적 특성이 우수함을 확인하였다.