• 제목/요약/키워드: DEG

검색결과 1,631건 처리시간 0.026초

가열멸균의 동력학적연구 (Studies on the Kinetics of the Heat Sterilization)

  • 우종학
    • 약학회지
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    • 제7권2_3호
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    • pp.42-50
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    • 1963
  • The destruction rate of microorganisms by heat follows the first order reaction. In this experiments, the calculated sterilization velocity constants of Escherichia Coli are 1.97 * $10^{-2}min^{-1}.(45{\deg}$ C), 9.53 * $10^{-2}min^{-1}.(55{\deg}$ C) and $1.858min^{-1}.(70{\deg}$ C), 2.89 * $10^{-2}min^{-1}.(80{\deg}$ C), 1.32 * $10^{-1}min^{-1}.(90{\deg}$ C), 2.87 * $10^{-1}min^{-1}.(95{\deg}$ C), 7.91 * $10^{-1}min^{-1}.(100{\deg}$ C) and $1.777min^{-1}.(105{\deg}$ C). In the results, the activation energy of E. Coli is 62.0 Cal $mole^{-1}.(45{\deg}-60-{\deg})$, that of B. Subtilis are 17.8 Cal $mole^{-1}(60{\deg}-80{\deg}$ C) and 47.1 Cal $mole^{-1}(90{\deg}-105{\deg}$ C).

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Differentially Expressed Genes of Potentially Allelopathic Rice in Response against Barnyardgrass

  • Junaedi, Ahmad;Jung, Woo-Suk;Chung, Ill-Min;Kim, Kwang-Ho
    • Journal of Crop Science and Biotechnology
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    • 제10권4호
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    • pp.231-236
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    • 2007
  • Differentially expressed genes(DEG) were identified in a rice variety, Sathi, an indica type showing high allelopathic potential against barnyardgrass(Echinochloa crus-galli(L.) Beauv. var. frumentaceae). Rice plants were grown with and without barnyardgrass and total RNA was extracted from rice leaves at 45 days after seeding. DEG full-screening was performed by $GeneFishing^{TM}$ method. The differentially expressed bands were re-amplified and sequenced, then analyzed by Basic Local Alignment Search Tool(BLAST) searching for homology sequence identification. Gel electrophoresis showed nine possible genes associated with allelopathic potential in Sathi, six genes(namely DEG-1, 4, 5, 7, 8, and 9) showed higher expression, and three genes(DEG-2, 3 and 6) showed lower expression as compared to the control. cDNA sequence analysis showed that DEG-7 and DEG-9 had the same sequence. From RT PCR results, DEG-6 and DEG-7 were considered as true DEG, whereas DEG-1, 2, 3, 4, 5, and 8 were considered as putative DEG. Results from blast-n and blast-x search suggested that DEG-1 is homologous to a gene for S-adenosylmethionine synthetase, DEG-2 is homologous to a chloroplast gene for ribulose 1,5-bisphosphate carboxylase large subunit, DEG-8 is homologous to oxysterol-binding protein with an 85.7% sequence similarity, DEG-5 is homologous to histone 2B protein with a 47.9% sequence similarity, DEG-6 is homologous to nicotineamine aminotransferase with a 33.1% sequence similarity, DEG-3 has 98.8% similarity with nucleotides sequence that has 33.1% similarity with oxygen evolving complex protein in photosystem II, DEG-7 is homologous to nucleotides sequence that may relate with putative serin/threonine protein kinase and putative transposable element, and DEG-4 has 98.8% similarity with nucleotides sequence for an unknown protein.

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공구 경사각의 변화에 따른 연질 재료(Cu alloy and Al alloy)의 절삭 특성에 관한 연구 (A study on the cutting character of soft materials(Cu alloy and Al alloy) with change of tool rake angles)

  • 염성하;현청남;오재응
    • 오토저널
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    • 제10권4호
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    • pp.85-96
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    • 1988
  • 제품생산의 고정밀화 및 고품질화로 정밀 절삭가공에서 공구의 형사에 따라 많은 변수가 있으므로 공구의 상면 경사각을 변화시키면서 절삭저항, 비절삭저항, 거칠기 등을 energy mothod에 의하여 최적조건을 구한 결과(6-4) brass 에서는 경사각 20.deg., 25.deg.에서 Al alloy 에서는 0.deg., 20.deg.에서 가장 작게 나타났으며 거칠기는 (6-4)brass 에서는 경사각 20.deg. 에서 Al alloy에서는 15.deg.에서 가장 작게 나타났다.

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핸드스프링 몸접어 앞공중돌기동작의 운동학적 분석 (The Kinematic Analysis of Handspring Salto Forward Piked)

  • 권오석
    • 한국운동역학회지
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    • 제17권1호
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    • pp.145-153
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    • 2007
  • The purpose of this study is to compare and analyze the phase-by-phase elapsed time, the COG, the body joint angle changes and the angular velocities of each phase of Handspring Salto Forward Piked performed by 4 college gymnasts through 3D movement analysis program. 1. The average elapsed time for each phase was .13sec for Phase 1, .18sec for Phase 2, .4sec for Phase 3, and .3sec for Phase 5. The elapsed time for Phase 1 to Phase 3 handspring was .35sec on average and the elapsed time for Phase 4 to Phase 5 handspring salto forward piked was .7sec on average. And so it showed that the whole elapsed time was 1.44sec. 2. The average horizontal changes of COG were 93.2 cm at E1, 138. 5 cm at E2, 215.7 cm at E3, 369.2 cm at E4, 450.7 cm at E5, and 553.1 cm at E6. The average vertical changes of COG were 83.1 cm at E1, 71.3 cm at E2, 78.9 cm at E3, 93.7 cm at E4, 150.8 cm at E5, and 97.2 cm at E6. 3. The average shoulder joint angles at each phase were 131.6 deg at E1, 153.5 deg at E2, 135.4 deg at E3, 113.4 deg at E4, 39.6 deg at E5, and 67.5 deg at E6. And the average hip joint angles at each phase were 82.2 deg at E1, 60 deg at E2, 101.9 deg at E3, 161.2 deg at E4, 97.7 deg at E5, and 167 deg at E6. 4. The average shoulder joint angular velocities at each phase were 130.9deg/s E1, 73.1 deg/s at E2, -133.9 deg/s at E3, -194.4 deg/s at E4, 29.4 deg/s at E5, and -50.1 deg/s at E6. And the average hip joint angular velocities at each phase were -154.7 deg/s E1, -96.5 deg/s at E2, 495.9 deg/s at E3, 281.5 deg/s at E4, 90.3 deg/s at E5, and 181.7 deg/s at E6. The results shows that, as for the performance of handspring salto forward piked, it is important to move in short time and horizontally from the hop step to the point to place the hands on the floor and jump, and to stretch the hip joints as much as possible after the displacement of the hands and to keep the hip joints stretched and high in the vertical position at the takeoff. And it is also important to bend the shoulder joints and the hip joints fast and spin as much as possible after the takeoff, and to decrease the speed of spinning by bending he shoulder joints and the hip joints quickly after the highest point of COG and make a stable landing.

쪼그려 앉은 자세에서의 최대 악력에 관한 연구 (A study of maximum grip strength on the squatted postures)

  • 김대성;김종인;양성환;박범
    • 대한인간공학회:학술대회논문집
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    • 대한인간공학회 1997년도 추계학술대회논문집
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    • pp.23-30
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    • 1997
  • 근골격계 질환은 부적절한 작업 자세, 중량물의 무리한 취급, 과도한 작업 부하 등과 같은 작업 환경 에서 기인한다. 현장의 대부분의 공구사용작업에 있어서, 쪼그려앉아서 작업하는 경우가 매우 빈번하게 발생한다. 본 연구에서는 쪼그려 앉은자세에서 사용하는 공구를 설계하는데 필요 요소인 악력의 최대치 를 조사하는 것을 목적으로 한다. 23세-29세의 남자 8명을 피실험자로 실험하였다. 실험모델로는 쪼그려 앉기에서 1) 0 .deg. 외전된 어깨와 정위치의 손목에서 어깨 굴곡이 180 .deg. , 135 .deg., 90 .deg. 와 45 .deg. 의 조합된 자세에서의 악력, 2) 0 .deg. 외전된 어깨와 정위치의 손목에서 팔꿈치 굴곡이 135 .deg. , 90 .deg. 와 45 .deg. 조합된 자세에서의 악력을 조사하였다. 조사결과 쪼그려 앉은 자세에서 어깨굴곡이 45 .deg. 인 자세에서 최대의 악력이 발휘되는 것으로 나타났으며, 인체부위 중에서 손너비, 손목둘레, 팔길이, 손두께, 팔꿈치-손목까지의 길이 등이 쪼그려 앉은자세와 깊은 상관관계가 있는 것으로 나타났다.

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Abridged Region from Escherichia coli Periplasmic Stress Sensor DegS Acts as Plasminogen Activator In Vitro

  • Junpeng, Yan;Ko, Juho;Qi, Yipeng
    • Journal of Microbiology and Biotechnology
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    • 제17권4호
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    • pp.594-599
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    • 2007
  • It is well known that the Escherichia coli inner membrane-bound protease DegS is a periplasmic stress sensor for unfolded outer membrane proteins (OMPs). Previous studies have also shown that the outer membrane protease OmpT activates plasminogen in vitro and this may be exploited by bacteria in the course of pathogenesis. However, there has been no research on the plasminogen activation ability of the important periplasmic protein DegS. Accordingly, in this study, the whole-length and truncated degS genes were separately overexpressed in Escherichia coli, the recombinant proteins purified by affinity chromatography, and their plasminogen activator role tested in vitro. The results suggested that the whole-length DegS was able to activate plasminogen on a plasma plate. The truncated form of DegS (residues 80-345), designated ${\Delta}DegS$, also acted as a plasminogen activator, as confirmed by different assays. The serine protease property of ${\Delta}DegS$ was verified based on the complete inhibition of its enzyme activity by PMSF (phenylmethanesulfonyl fluoride). Therefore, the present results indicate that DegS is a plasminogen activator in vitro.

보와 판이론에 의한 보형상 복합재의 충격 거동에 관한 연구 (A Study on the Impact Behavior of the Beam-Like Laminated Composite by the Beam and Plate Theories)

  • 김문생;안국찬;김규남
    • 대한기계학회논문집
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    • 제13권4호
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    • pp.643-652
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    • 1989
  • 본 연구에서는 저자들에 의해 개발된 보와 판의 동적 유한 요소법으로 보형상을 지닌 적층 복합재의 형상비(길이/폭)와 적층 형태의 변화에 따른 충격 해석시 어느 이론의 결과가 더욱 적합한지에 대한 타당성 여부를 검토하고자 한다.

InP기반 InAs 2DEG HEMT성장 및 전기적특성

  • 송진동;신상훈;김수연;이은혜
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2009년도 제38회 동계학술대회 초록집
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    • pp.168-168
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    • 2010
  • InAs는 0.35eV의 낮은 밴드갭을 가지며 상온에서 약 $30,000cm^2/Vs$의 높은 전자이동도를 보여, GaAs/AlGaAs 및 InGaAs/InP 2DEG HEMT에 이은 차세대 초고속 전자소자의 2DEG용 물질로 각광을 받고 있다. 그러나 InAs의 격자상수는 약 0.61nm로 이에 적절한 반절연기판을 구할수 없어, GaAs상에 Al(Ga)Sb를 이용하여 성장하는 방법으로 2DEG을 실현하고 있다. 상기 방법으로 상온에서 ${\sim}30,000cm^2/Vs$ 전자이동도를 보이는 InAs/AlSb 2DEG HEMT 소자를 여러 연구팀에서 시현하였으나, 실제적으로 응용하기 위해서 etch-stop층 또는 contact층의 제작이 용이치 않아 실제의 회로구현에는 어려움을 격고 있다. 이에 InGaAs/InP 2DEG내에 InAs를 넣어 InAs 2DEG을 제작하는 방법이 NTT[1]에 의해 제안되어, SPINTRONICS등의 InAs 2DEG이 필요한 곳에 응용되고 있다. [2] 본 발표에서는 고품질의 InAs 2DEG을 실현하기 위해, 다양한 성장 변수 (온도, As 분압, 성장 시퀀스, InAs층의 두께등)와 2DEG의 전기적특성간의 관계를 발표한다. 최종적으로 상온전자이동도 ${\sim}12,000cm^2/Vs$의 InAs 2DEG을 제작할수 있었으며, 이를 다양한 전자소자에 차후 응용할 예정이다.

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複合積層板 의 Delamination 解析 (Analysis of Delamination in Laminated Composites)

  • 김광수;홍창선
    • 대한기계학회논문집
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    • 제6권2호
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    • pp.140-146
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    • 1982
  • The purpose of this study is to investigate the free edge delamination of the laminated composites under uniaxial strain. The laminate is modeled as a set of anisotropic layers with isotropic adhesive layers. Interlaminar stresses are calculated for laminate with various laminate parameters by using two dimensional finite difference method. The redistribution of interlaminar stresses after delamination and the relation between delamination any ply failure are obtained for [.+-.45.deg.]$_{s}$, [0.deg./.+-./.+-.45.deg./90.deg.]$_{s}$ and [0.deg./45.deg./90.deg/45.deg.]$_{s}$ laminates. It was found that delamination can not propagate the entire width of the laminate under the static loading condition.ition.

圓孔 이 있는 有限 直交異方性 Graphite / E Laminate 의 應力集中係數 (Stress concentration factors for finite orthotropic graphite/E laminates with a circular hole)

  • 홍창선
    • 대한기계학회논문집
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    • 제4권3호
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    • pp.113-118
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    • 1980
  • Stresses were calculated for finite-width orthotropic laminates with a circular hole and remote uniaxial loading using a two-dimensional finite-element analysis with both uniform stress and uniform displacement boundary conditions. Five different laminates were analyzed: quasi-isotropic [0.deg./.+-.45.deg./90.deg.].$\_$s/, 0.deg., 90.deg., [0.deg./90.deg.]$\_$s/, and [.+-.45.deg.]$\_$s/, Computed results are presented for selected combinations of hole diameter-sheet-width ratio d/w and length-to width ratio L/w. For small L/w values, the stress-concentration factors K$\_$tn/ were significantly different for the uniform stress and uniform displacement boundary conditions. Typically, for the uniform stress conditions, the K$\_$tn/ values were much larger than for the infinite-strip reference conditions; however, for the uniform displacement conditon, they were only slighty smaller than for this reference. The results for long strips are also presented as width-correction factor. For d/w.leg.33, these width-correction factors are nearly equal for all five laminates.