• Journal of the Society of Cosmetic Scientists of Korea
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• v.34 no.2
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• pp.137-142
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• 2008

Echinacea purpurea, an indian traditional plant medicine has been widely used as herbal remedy for the treatment of disease such as colds or other infections. However, Echinacea purpurea extracts recently have been applied as a cosmetic ingredient for skin care. We artificially cultured Echinacea purpurea by using the bioreactor culture system for this study. We induced callus from Echinacea purpurea and separated adventitious roots, harvested and extracted after cultured in bioreactors. Previously, several studies have been reported on anti-oxidant and immuno-enhancing effects of Echinacea purpurea extract but other efficacies were not well known. In this study, we investigated the whitening, anti-wrinkle and anti-oxidant effects to know applicable value of tissue-cultured Echinacea purpurea adventitious roots extract(TCEPARE) as a cosmetic ingredient. TCEPARE did not show cytotoxicity until a concentration of 2% and showed the anti-oxidative effect in DPPH and NBT tests. Also, the extract decreased tyrosinase expression in a dose-dependent manner and inhibited melanin synthesis in B16 melanoma cells. TCEPARE reduced protein levels of MMP-1, 2 secreted in culture medium or in cell lysates. From these results we suggest that TCEPARE has potential benefits applicable as to cosmetic ingredient for skin care products.

• Korean Journal of Medicinal Crop Science
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• v.12 no.4
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• pp.309-314
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• 2004

The cytotoxic effects of water and ethanol extracts of Echinacea purpurea (L.) (EP) and chloroform, ethyl acetate, butanol and aqueous fractions from each extract of EP were examined. Every extract and fraction of EP inhibited the growth of human hepatocarcinoma, human gastric cancer cell, human breast cancer cells and human lung carcunoma in concentration-dependent manners over a concentration range of $0.05{\sim}1.0\;mg/ml$. Most extracts and fractions with the concentraction of 1 mg/ml showed strong inhibition of more than 70% for every cancer cell. Only aqueous fractions of each extract showed very weak inhibitons of 12 to 25% on the growth of human normal lung cell with the concentration of 1 mg/ml. Overall selectivity of the extrats and fractions on the four human cancer cell lines was over 2.5. These results indicate that EP has a very potent selective toxicity for cancer cells.

• Korean Journal of Medicinal Crop Science
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• v.10 no.1
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• pp.51-57
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• 2002

The biological activities of immune modulating activities of the extracts from Echinacea purpurea, Chrysanthmum indicum L. and Circium japonicum var. ussuriense KITAMURA were compared. About 70% of the growth of human hepatocarcinoma and 80% of human gastric cancer cell was inhibited in adding 0.5mg/ml of the ethanol extracts of Echinacea purpurea, Chrysanthmum indicum L. and Circium japonicum var. ussuriense KITAMURA, respectively. The growth of human breast cancer cells was also inhibited in adding 0.5mg/ml of the extracts as well as 60% of the human cancer cells. It was proved that the growth of human normal lung cell, scored as 15% for the extracts. Overall selectivity of the extracts on several human cancer cell line was over 3, which is higher than those from the conventional herbs. The growth of both human immune B and T cells was enhanced up to 1.4 to 2.0 times by adding the extracts, compared to the controls. The secretion of tumor necrosis $factor-alpha(TNF-{\alpha})$ from T cell was also increased up to 94 pg/ml in adding the Echinacea purpurea ethanol extract (0.5mg/ml). Circium japonicum var. ethanol extract also increased up to about 96 pg/ml of interleukin-6(IL-6) from B cell.

• Korean Journal of Organic Agriculture
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• v.25 no.4
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• pp.821-830
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• 2017

In this study, the DPPH free radical scavenging activity, antimicrobial effects, growth inhibition and cytotoxicity of herb extracts were determined to screen alternative antibiotics. Hot water extracts of 10 species herbs (Origanum vulgare, Monarda didyma, Echinacea purpurea, Ocimum basilicum, Mentha piperita, Melissa officinalis, Thymus vulgaris, Stevia rebaudiana, Rosmarinus officinalis, Matricaria recutica) were used. DPPH free radical scavenging activity of all herb extracts was ranged from 31.4-49.9%, and significantly great activties were found at Rosmarinus officinalis, Origanum vulgare and Matricaria recutica (P<0.05). Hot water extracts of monarda didyma, origanum vulgare, thymus vulgaris and rosmarinus officinalis showed greater antimicrobial activities compared to others. Additionally, those four extracts represented relatively low cytotoxicity compared to others. As a result, it was found that Origanum vulgare and Rosmarinus officinalis which possessed great antioxidant and antimicrobial activity with less cytotoxicity. So these two herb extracts can be used as an alternative of antibiotics for organic farming.