• 제목/요약/키워드: Escherichia coli

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Portulaca oleracea L. 추출물에 의한 Escherichia coli KCTC 2441의 생육억제

  • 이은숙;서부일
    • 대한한의학방제학회지
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    • 제9권1호
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    • pp.367-374
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    • 2001
  • 쇠비름 추출물의 농도가 1%정도부터 Escherichia coli KCTC 2441의 성장 억제 효과가 나타났으며 7%일 때 균의 성장이 급속도로 억제되었다. 즉 쇠비름 추출물이 Escherichia coli KCTC 2441에 대하여 항균작용을 나타내고 있음이 증명되었다. 6월에 양지 바른 곳에서 자란 쇠비름이 대장균 성장 억제 효과가 높았으며 잎보다는 줄기 부분이 잎 중에서도 적자주색을 띠는 잎이 대장균 성장 억제 효과가 높았다.

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Optimal culture conditions for production of Escherichia coli Adhesin protein coupled to Escherichia coli Heat Labile Enterotoxin A2B in Escherichia coli TB1.

  • Lee, Yong-Hwa;Rhee, Dong-Kwon;Pyo, Suhk-Neung
    • 대한약학회:학술대회논문집
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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    • pp.226.2-226.2
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    • 2003
  • The FimH subunit of type 1-fimbriated Escherichia coli has been determined as a major cause of urinary tract infection. To produce a possible vaccine antigen against urinary tract infection, the fimH gene was genetically linked to the Itxa2b gene, which was then cloned into the pMAL -p2E expression vector. The chimaeric construction of pMALfimH/Itxa2b was transformed into Escherichia coli TB1 and its N-terminal amino acid sequence was analyzed. (omitted)

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구형 ZrO$_2$를 충진한 방전관의 수질 변환 및 대장균제거 특성 (Property Conversion of Water and Removing Characteristics of Escherichia Coli Inner Discharge Tube With ZrO$_2$Beads)

  • 이동훈;박재윤
    • 대한전기학회논문지:전기물성ㆍ응용부문C
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    • 제53권3호
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    • pp.143-148
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    • 2004
  • This paper shows property conversion of water and removing characteristics of Escherichia coli for discharge tube with $ZrO_2$ beads. At the result of the removal characteristic experiments of Escherichia coli using the discharge tube with $ZrO_2$ beads, because the electric field is also increased when input voltage is increased, the removal characteristic of Escherichia coli was appeared relation connection to input voltage. And if a passing number of test water in discharge tube with $ZrO_2$beads is increased, the removal ratio of Escherichia coli is to be increased because passing number of electric field section is increased. And if diameter of $ZrO_2$beads is increased, the removal time of Escherichia coli is to be decreased because dielectric polalization of $ZrO_2$beads. Also, the removal ratio of Escherichia coli of the discharge tube with $ZrO_2$beads. is appeared higher than the removal ratio of the discharge tube without $ZrO_2$beads. And a satulation punt of ozone and $H_2$ $O_2$generation density inner water was appeared near 60[min].

구형 $SiO_2$를 갖는 방전관의 수중 세균제거특성 (Removal characteristic of Escherichia coli for Discharge tube with globular $SiO_2$)

  • 이동훈;박홍재;박재윤
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2003년도 추계학술대회 논문집 Vol.16
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    • pp.546-549
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    • 2003
  • This paper was shown removal characteristic of escherichia coli for discharge tube with globular $SiO_2$. At the result of the removal characteristic experiments of escherichia coli using the discharge tube with globular $SiO_2$, because the electric field is also increased when input voltage is increased, the removal characteristic of escherichia coli was appeared relation connection to input voltage. If a passing number of test water in discharge tube is increased, the removal ratio of escherichia coli is increased because passing number of electric field section is increased. When diameter of globular $SiO_2$ is increased, the removal time of escherichia coli is to be decreased because dielectric polalization of globular dielectric($SiO_2$), Also, the removal ratio of escherichia coli of the discharge tube with globular dielectric($SiO_2$) is appeared higher than the removal ratio of the discharge tube without globular dielectric($SiO_2$).

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구형 SiO$_2$를 갖는 수방전관의 전계 해석 및 대장균 제거 특성 (Electric Field Analysis and Removal Characteristics of Escherichia Coli for Water Discharge Tube with Globular SiO$_2$)

  • 이동훈
    • 대한전기학회논문지:전기물성ㆍ응용부문C
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    • 제53권2호
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    • pp.103-108
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    • 2004
  • This paper shows the simulation of electric field distribution and removal characteristics of Escherichia coli for water discharge tube with globular $SiO_2$. At the experiments of the removing Escherichia coli used the discharge tube with globular dielectric($SiO_2$), because the electric field is increased when applied voltage is increased, the removed ratio of Escherichia coli was related with increasing of applied voltage. When a passing number of test water in water discharge tube is increased, the removed ratio of Escherichia coli is increased because passing number of territory with electric field is increased. When diameter of globular dielectric($SiO_2$) is increased, the removed time of Escherichia coli was decreased because electric field for dielectric polarization of globular dielectric($SiO_2$) was increased. Also, the removed ratio of Escherichia coli of the water discharge tube with globular dielectric($SiO_2$) was measured higher than the removal ratio of the discharge tube without globular dielectric($SiO_2$)

알칼리조건에서 Escherichia coli 0157:H7의 적응 및 항균요법제에 대한 감수성 (Habituation of Escherichia coli O157:H7 in Alkali and Susceptibility of Causative Agents)

  • 배종호
    • 한국식품영양학회지
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    • 제12권6호
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    • pp.564-568
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    • 1999
  • Habituation to alkali condition and susceptibility to antimicrobial agents were investigated to determine methods t inactivation Escherichia coli O157:H7 associated with food poisoning. The investigation showed that Escherichia coli O157:H7growth at pH 9.0 The susceptibilities of Escherichia coli O157:H7 to 13 drugs examined were found highest in regad to saicin and ceftriaxone and lowest in regard to erythromycin and josamycin. No significant changes in minimal inhibitory concen-tration(MIC) values of bactericidal effect was found when saicin and ceftriaxone were irradiated with doses of 5-100KGy.

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닭유래 Escherichia coli의 병원성에 관한 연구 : 시험관내 Congo-red 결합능과 병원성간의 상관관계 (Studies on pathogenicity of Escherichia coli isolated from chickens: Correlation between in vitro Congo-red binding properties and in vivo virulence in avian Escherichia coli)

  • 우용구;김기석;김봉환
    • 대한수의학회지
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    • 제31권1호
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    • pp.55-61
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    • 1991
  • The correlation between in vitro Congo-red binding properties of E coli and in vivo invasiveness of the organisms in SPF chickens and mice was investigated. Congo-red positive E coli colonies were dark-red color with a typical colonial morphology of rough appearance when grown on Congo-red medium, while Congo-red negative colonies showed pale-pink color and smooth surfaced colonial morphology. Pathogenicity of 10 Congo-red positive E coli for mice was observed in 92.5% but that of 5 Congo-red negative E coli was 45%. Invasiveness of 10 Congo-red positive E coli for chickens was observed in 96% of the SPF chickens tested but that of 5 Congo-red negative E coli was 16% only. These results of pathogenicity studies with E coli isolates indicate a significant correlation between Congo-red binding ability and virulence in avian Escherichia coli.

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YRp 7 vector를 이용한 Bacillus amyloliquefaciens amylase gene의 cloning I. Escherichia coli에서의 발현 (Cloning of Bacillus amyloliquefaciens amylase gene using YRp7 as a vector I. Expression of cloned amylase gene in Escherichia coli)

  • 서정훈;김영호;전도연;홍순덕;조윤래
    • 한국미생물·생명공학회지
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    • 제14권2호
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    • pp.161-168
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    • 1986
  • E. coli-S. cerevisiae shuttle vector인 plasmid YRp7을 이용하여 B. amyloliquefaciens의 $\alpha$-amylase gene을 E. coli 내에 cloning하였다. 이때 제한 효소 Sau 3 AI에 의해 얻어진 $\alpha$-amylase gene의 크기는 약 1.95kb정도였으며 E. coli내에서 비교적 안정하게 유지되고 발현되었다. 재조합 plasmid p-EA24를 함유한 E. coli는 B. amyloliquefaciens의 약 65% 정도의 $\alpha$-amylase를 생성하였으며, 최적온도, pH, CaCl$_2$의 영향등 $\alpha$-amylase의 효소학적인 성질을 비교 조사해 본 결과 B. amyloliquefaciens의 $\alpha$-amylase와 동일하였다. 또한 E. coli에서 생성된 $\alpha$-amylase로 70% 정도가 periplasmic space에 존재하였으며 나머지는 세포 내부에 존재함을 알았다.

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Optimized Culture Conditions for Production of the chimaeric protein, Uropathogenic Escherichia coli Adhesin - Cholera Toxin A2B Subunits, in Escherichia coli TB1

  • Lee, Yong-Hwa;Kim, Byung-Oh;Rhee, Dong-Kwon;Pyo, Suh-Kneung
    • Biomolecules & Therapeutics
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    • 제12권3호
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    • pp.179-184
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    • 2004
  • The FimH subunit of type 1-fimbriated Escherichia coli has been determined as a major cause for urinary tract infections. In our previous study, the Adhesin/CTXA2B was expressed as soluble recombinant chimaeric protein derived from the uropathogenic Escherichia coli adhesin genetically coupled to cholera toxin A2B (CTXA2B) subunit in Escherichia coli. Since it is very important to optimize IPTG concentration and culture temperature to maximize cell growth and productivity, These optimal culture factors were determined to increase the productivity of the expressed Adhesin/CTXA2B chimaeric protein in Escherichia coli TB1 carrying pMALfimH/ctxa2b. Our data demonstrate that optimal concentration of IPTG for increased production of chimaeric protein was 0.5 mM. Additionally, culture time was 10 hours and temperature, 37${\circ}C$.

구형 지르코니아를 갖는 수방전관의 수질성분변환 및 세균제거특성 (Removal characteristic of Escherichiacoli and water property conversion for Discharge tube with globular $ZrO_2$)

  • 이동훈;박홍재;박재윤
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2003년도 추계학술대회 논문집 Vol.16
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    • pp.550-553
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    • 2003
  • This paper was shown water properly conversion and removal characteristic of escherichia coli for discharge tube with $ZrO_2$ beads. At the result of the removal characteristic experiments of escherichia coli using the discharge tube with $ZrO_2$ beads, because the electric field is also increased when input voltage is increased, the removal characteristic of escherichia coli was appeared relation connection to input voltage. And if a passing number of test water in discharge tube with $ZrO_2$ beads is increased, the removal ratio of escherichia coli is to be increased because passing number of electric field section is increased. And if diameter of $ZrO_2$ beads is increased, the removal time of escherichia coli is to be decreased because dielectric polalization of $ZrO_2$ beads. Also, the removal ratio of escherichia coli of the discharge tube with $ZrO_2$ beads. is appeared higher than the removal ratio of the discharge tube without $ZrO_2$ beads. And a salutation point of ozone and $H_2O_2$ generation density inner water was appeared near 60[min].

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