• 대한한의학회지
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• 제19권2호
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• pp.50-58
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• 1998

The effects of the Evodiae fructus on the cardiovascular function were assessed in spontaneously hypertensive rats, and isolated preparation of spontaneously hypertensive rats aortic strip. In spontaneously hypertensive rats, intraperitoneal administration of methanol crude extract of Evodiae Fructus (0.5mg/kg) lowered aterial blood pressure which lasted for at least 4 hours. The hypotensive effect of Evodiae Fructus was more stronger with ${\alpha}-adrenoreceptor$ inhibitor(Phentholamine) and it was not affected by ${\beta}-adrenoreceptor$ inhibitor(Propranolol). The hypotensive effect of Evodiae Fructus was abolished by NAME. It is suggested that the hypotensive effect of Evodiae Fructus may be concern with endothelium-derived relaxing factor and it may be mediated through NO synthesis. Evodiae Fructus showed a vasorelaxing effect on denude aortic strip. It is suggested that Evodiae Fructus has a direct relaxing effect on vascular smooth muscle cells. Judging from above results, it was proved that hypotensive effect of Evodiae Fructus. The mechanism of hypotensive action may be concern with endothelium-derived relaxing factor and direct relaxing effect on vascular smooth muscle cells. Therefore, it is suggested that Evodiae Fructus is applicable to hypertension.

• Biomolecules & Therapeutics
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• 제8권4호
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• pp.305-310
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• 2000

The present study examined the effect of a methanol extract of Evodiae Fructus on the blood pressure in spontaneously hypertensive rats (SHR). The systolic blood pressure was measured after rats were pretreated with phentolamine, propranolol, or $N_{\omega}$-nitro-$_{L}$-arginine methyl ester(NAME) and subsequently received methanol extract of Evodiae Fructus. In SHR, intraperitoneal administration of methanol extract of Evodiae Fructus (0.5 mg/kg) produced antihypertensive effect that lasted for at least 4 hours. Antihypertensive effect of Evodiae Fructus was more stronger than that with $\alpha$-adrenergic receptor antagonist phentolamine and was not affected by $\beta$-adrenergic receptor antagonist propranolol. The antihypertensive effect of Evodiae Fructus was abolished by pretreatment of NAME. Our findings suggest Evodiae Fructus has an hypertensive effect, which may be mediated through nitric oxide synthesis.s.

• 치위생과학회지
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• 제3권1호
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• pp.39-44
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• 2003

본 연구는 오미자, 오수유의 추출물의 S. mutans에 대한 항균효과를 paper disc method로 검색하여, S. mutans 생장에 관한 영향을 평가하였다 1. 오미자, 오수유 열수 추출물의 항균효과를 탐색한 결과 S. mutans에 대한 항균력이 우수한 순서는 오미자, 오수유 순이었으며, paper disc method에 의한 항균활성 측정(20 mg/ml 투여) 결과는 오미자가 8.5mm, 오수유가 6.5mm 이었다. 2. 한약재 추출물의 S. mutans 생장에 대한 효과는 대조군이 8시간에서 1.05로 최대 생장률을 보였으나, 오미자, 오수유 추출물을 2 mg/ml을 투여한 배지에서는 16시간만에 0.90, 0.80로 최대생장을 보였다. 3. S. mutans를 8시간 동안 배양한 후 배지내 pH를 측정한 결과 대조군은 5.08 이었으며, 오미자, 오수유 추출물이 투여된 배지는 6.00, 5.36 이었다. 4. S. mutans를 8시간 동안 배양한 후 배지내 탄수화물 변화량을 측정한 결과 대조군이 0.81 mg/ml 이었으며, 오미자, 오수유 추출물이 투여된 배지는 1.32 mg/ml, 1.88 mg/ml 이었다. 5. S. mutans를 8시간 동안 배양한 후 배지내 단백질 변화량을 측정한 결과 대조군은 8.39 mg/ml 이었으며, 오미자, 오수유 추출물이 투여된 배지는 8.59 mg/ml 9.97 mg/ml 이었다. 6. S. mutans의 다당류 생성에 미치는 효과는 대조군의 경우 배양 8시간에 300 mg/ml을 생성하였으나, 오미자, 오수유 추출물이 투여된 배지에서는 240 mg/100 ml, 280 mg/100 ml을 생성하였다.

• 대한한방내과학회지
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• 제29권4호
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• pp.1000-1010
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• 2008

Objective : Hypothyroidism is a syndrome characterized by symptoms such as cold intolerance, or weight gain. Because of side effects of Western medicine treatment, interest in oriental medicine has been increasing. In this study, we examined the therapeutic effects of Evodiae fructus and histological modification in hypothyroidism rat model induced by PTU(6-propyl, 2-thiouracil). Methods : After inducing hypothyroidism in the rats by PTU injections, we divided the rats into four groups, the Evodiae fructus 100 500, control, and $LT_4$ (levothyroxine) groups. After 2 weeks Evodiae fructus and $LT_4$ were administered, respectively. The weight was measured once a week. After sampling the blood for biochemical analysis to check the levels of $T_3$, $T_4$ and TSH, the thyroid tissue was removed, stained with H&E, and an image was obtained using an optical microscope. Results : Compared with normals, controls showed low $T_4$ and high TSH. The Evodiae fructus group showed a statistically meaningful $T_3$ increase to be dose related. But the levels of TSH showed no meaningful difference between the Evodiae fructus group and normals. About the biochemical tests(liver, kidney etc) and weight change, there was no meaningful difference between the Evodiae fructus group and controls. In the biopsy, the Evodiae fructus group showed thyroid tissue improvement compared to controls. Conclusions : These results show that Evodiae fructus stimulates the decreased functions of the thyroid, and also has thyroid cell protecting effects. The lab results also showed that Evodiae fructus is a safe substance that doesn't cause hepatotoxicity or nephrotoxicity. Therefore it is an effective substance in the treatment of hypothyroidism.

• 대한한방부인과학회지
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• 제22권2호
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• pp.60-78
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• 2009

Purpose: These experiments were undertaken to evaluate the effect of administration of Evodiae Fructus on ovarian functions and differential gene expressions related caspase-3, MAPK and MPG in female mice. Methods: We administered the Evodiae Fructus to 6-week-old female ICR mice for 4, 8, or 12 days. With different concentration of Evodiae Fructus, the female mice were injected PMSG and hCG for ovarian hyperstimulation. The mice divided into 3 groups for each experiment. We chose the caspase-3 for cell apoptosis, MAPK and MPG genes for cell viability and DNA repair. Results: In case of 4, 8, 12 day of Evodiae Fructus, we were examined the mean number of total ovulated oocytes and the number of morphologically normal oocytes. We were also examined the embryonic developmental competence in vitro. In addition we were also examined the differential expression of cell viability related genes, caspase-3, MAPK and MPG according to concentration and duration of Evodiae Fructus administration. MPG gene expressions for cell viability and DNA repaie were increased in dose dependent manner than that of control group in 4-day administration group. Conclusion: It is suggested that the medication of Evodiae Fructus has beneficial effect on reproductive functions of female mice via promotion of cell proliferation.

• 약학회지
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• 제43권3호
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• pp.397-403
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• 1999

The present study examined the effect of a methanol extract of Evodiae Fructus on the cardiovascular function in N-nitro-L-arginine methyl ester (NAME)-induced hypertensive Wistar rats after treatment over 6 weeks. In rats treated with NAME, blood pressure, weight of heart, aorta media thickness and media/lumen ratio significantly (p<0.05) increased, whereas coronary flow and heart rate of isolated heart significantly (p<0.05) decreased compared with control group at 6 weeks. In rats treated with NAME and Evodiae Fructus, blood pressure, aorta media/lumen ratio significantly(p<0.05) decreased compared with NAME treated group at 6 weeks. These results suggest that Evodiae Fructus is applicable to the treatment of hypertension and vascular hypertrophy.

• 대한한의학방제학회지
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• 제23권2호
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• pp.245-259
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• 2015

Objectives : This study is intended to establish the areas of use of the major effects of Coptidis Rhizoma-Evodiae Fructus Pair in the Prescriptions. Methods : Prescriptions that include both Coptidis Rhizoma and Evodiae Fructus have been selected with the use of programs offered by http://www.koreantk.com, http://youl.net/data . This study compared and analyzed the main effects of the selected prescriptions. Results : Prescriptions that work on upper digestive system tend to have Coptidis Rhizoma to Evodiae Fructus ratio of 2:1~10:1, and Prescriptions that work on lower digestive system tend to have ratio of 1:1. When Coptidis Rhizoma takes up 50% or more of the total dose of the prescriptions, these prescriptions tend to have major effect on the upper digestive system, and when it takes less than 50%, these tend to have major effect on the lower digestive system on the contrary. Conclusions : The prescriptions based on Coptidis Rhizoma-Evodiae Fructus Pair have a tendency to treat digestive problems caused by mass of fever in Liver-system. On this basis, further studies are expected to unveil the effectiveness of these prescriptions on adult diseases.

• 대한의생명과학회지
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• 제27권2호
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• pp.77-87
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• 2021

Reflux esophagitis (RE) is a disease that stomach contents, stomach acid, and pepsin continually refluxing and is curently increasing worldwide. This study was conducted to find natural materials that can reduce side effects and effectively treat RE. Animal experiments were conducted with a 1:1 (EA1), 1:5 (EA5) ratio of Evodiae Fructus and Arecae Semen known to be effective against reflux esophagitis. As a result of confirming the total lesion of the esophageal mucosa after EA1 or EA5 treatment in reflux esophagitis animals, it showed superior improvement compared to the RE-control rats. In addition, by regulating the expression of MPO and NADPH oxidase, the activation of NF-κB was inhibited, and the expression of COX-1 and COX-2 was regulated. Moreover, its improved esophageal barrier function through regulating protein expressions of tight junction protein and MMPs/TIMPs. Taken together, a mixture of Evodiae Fructus and Arecae Semen can attenuate the damage to the esophageal mucosa that not only inactivationed the NF-κB through oxidative stress control, but also by regulating tight junctions and MMPs/TIMPs. This effect was more excellent in the 1:1 mixture (EA1) than in the Evodiae Fructus and Arecae Semen 1:5 mixture (EA5).

• 생약학회지
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• 제35권1호통권136호
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• pp.88-91
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• 2004

Abstract - The Evodiae Fructus is known for containing a number of indolquinazoline and quinoline type alkaloids. Evodiamine, evocarpine and rutaecarpine are the major constituents of alkaloids. These alkaloids were isolated and determined by forming complex compounds from Evodiae Fructus in O-Su-You-Tang. For the determination of these alkaloids, a new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate[II] compIεx compound ion. The absorbance of alkaloidal complex compounds in 1,2-dichloroethane solution was measured at 625 nm. The method proved to be rapid, simple and reliable for the isolation and the determination of the alkaloids in O-Su-You-Tang.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.202-202
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• 1996

The antihistaminic action of eighteen herbal medicines was investigated by the radioligand binding and functional assays. The hexane fractions of Trichosanthis radix, Mori cortex radicis and Evodiae fructus dose-dependently inhibited [$^3$H]mepyramine binding to H$_1$ receptor and histamine-induced contraction in guinea-pig brain homogenates and isolated guinea-pig ilea, respectively. Antihistaminic action of the hexane and ethylacetate fractions of Mori cortex radicis and the hexane fraction of Evodiae fructus was more potent than their antimuscarinic action evaluated from the inhibition of [$^3$H]QNB binding and carbachol response. The ethylacetate and chloroform fractions and six known flavonoids from Scutellariae radix also inhibited histamine-induced contraction, but antihistaminic potencies of these fractions and compounds were almost identical with their antimuscarinic potencies. The hexane fractions of Mori cortex radicis and Evodiae fructus, as shown in ketotifen, inhibited selectively the increase of cutaneous vascular permeability induced by histamine. However, wogonin (SC-1) from Scutellariae radix was a nonselective inhibitor for the effect of histamine and serotonin on the vascular permeability. These results demonstrate that the hexane and ethylacetate fractions of Mori cortex radicis and the hexane fraction of Evodiae fructus have the selective histamine H$_1$ receptor blocking activities.