• 한방안이비인후피부과학회지
• /
• 제15권1호
• /
• pp.31-49
• /
• 2002

This Experimental study was done to research effects of Fagopyrum esculentum Moench(Me-mil) Extract on the anti-allergic effects. The results were obtained as follows : 1. All concentrations of Fagopyrum esculentum Moench Extract(Flours and seeds) inhibit histamine release under the vascular permeability response to intracutanenous injection. The result is proportion to concentration. But, rutin can't get considerable result. 2. All concentrations of Fagopyrum esculentum Moench Extract inhibit histamine release under the vascular permeability response to intraperitoneal injection of SD Rat in comparison with Diphenhydramine which is typical anti-histamine drug. 3. All concentrations of Fagopyrum esculentum Moench Extract inhibit histamine release under the vascular permeability response to per-oral during the three or five days. 4. In the result of quantification of histamine induced Compound $\frac{48}{80}$, Flours and seeds of Fagopyrum esculentum Moench Extract inhibit histamine release. 5. Among the fracination of Fagopyrum esculentum Moench Extract, $CHCl_3$ fraction inhibit histamine release effectively. 6. In the result of genetic manifestative inhibition about the Human mast cell treated PMA and A23187, Fagopyrum esculentum Moench Extract effect in the IL-4 and TNF-${\alpha}$ except IL-5. According to the above results, it is suggested that Fagopyrum esculentum Moench(Me-mil) Extract has anti-allergic effect.

• 동의생리병리학회지
• /
• 제21권5호
• /
• pp.1243-1249
• /
• 2007

The aim of this study was to investigate the effect of ethanol extract of Fagopyrum esculentum on the melanogenesis. To determine whether ethanol extract of Fagopyrum esculentum suppress melanin synthesis in cellular level, B16F10 melanoma cells were cultured in the presence of different concentrations of Fagopyrum esculentum ethanol extract. In the present study, we examined the effects of Fagopyrum esculentum ethanol extract on cell proliferation, melanin contents, tyrosinase activity, expression of melanogenic enzyme proteins including tyrosinase, tyrosinase-related protein 1 (TRP-1) and tyrosinase-related protein 2 (TRP-2). Cell proliferation was slightly increased by treatment with ethanol extract of Fagopyrum esculentum $(25-200 {\mu}g/m{\ell}).$ The ethanol extract of Fagopyrum esculentum effectively suppressed melanin contents at a dose of $100 {\mu}g/m{\ell}).$ It was observed that the color of cell pellets was totally whitened compared with the control. The ethanol extract of Fagopyrum esculentum inhibited tyrosinase activity, regulate melanin biosynthesis as the key enzyme in melanogenesis. Using western blot analysis, the ethanol extract of Fagopyrum esculentum dose-dependently decreased tyrosinase and TRP-1 protein levels, and tyrosinase and TRP-1 were detected in similar manner. ${\alpha}-MSH$ leads to a stimulation of melanin synthesis through increase of tyrosinase activity, melanin contents and cytoplasmic dendricity. In this study, ethanol extract of Fagopyrum esculentum down-regulated the ${\alpha}-MSH$-induced tyrosinase activity, melanin contents and cytoplasmic dendricity. Regarding protein levels of the melanogenic enzymes, the amounts of tyrosinase and TRP-1 was increased after incubation with a-MSH. The treatment of ethanol extract of Fagopyrum esculentum decreased the ${\alpha}-MSH$-induced expression levels of tyrosinase and TRP-1. These results suggest that the ethanol extract of Fagopyrum esculentum exerts its depigmenting effects through the suppression of tyrosinase, TRP-1 and cytoplasmic dendricity. And it may be a potent depigmetation agent in hyperpigmentation condition.

• 대한환경공학회지
• /
• 제30권11호
• /
• pp.1154-1160
• /
• 2008

아연 폐광지 부근의 논토양에서 메밀(Fagopyrum esculentum)을 이용한 식물상추출공법 적용 전후에 대해 토양 내 아연 형태 및 탈수소효소활성도 변화를 조사하였다. 메밀을 식재한 논토양은 아연농도는 약 600 mg/kg인 고오염 구역과 약 300 mg/kg인 저오염 구역으로 구분되었다. 메밀을 재배한 결과, 메밀은 고농도 구역에서 아연에 의한 성장 저해를 입었으나 토양으로부터 식물유효성 형태의 아연을 흡수하여 식물체내에 축적하였고, 아연 독성으로 저해된 토양 내 탈수소효소활성도를 증가시킨 것으로 나타났다. 2개월 동안 메밀을 재배한 후 근권 토양 내 식물유효성 아연 농도는 고오염 구역에서 약 25% 감소, 저오염 구역에서 약 70% 감소하였다. 근권 토양에서 감소한 식물유효성 아연의 양은 식물체내에 축적된 아연의 양과 유사하여(Recovery 92$\sim$107%), 메밀이 토양 내 식물유효성 아연을 제거 및 축적하였음을 알 수 있었다. 또한 토양 내 총 아연 농도와 토양 탈수소효소활성도는 음의 상관관계를 가진 것으로 나타났으며, 초기에 고오염 토양에서는 저오염 토양에 비하여 토양 탈수소효소활성도가 약 2배 저해되었지만 메밀 재배 후 각각의 근권에서 약 35% 증가한 것을 확인하였다.

• 대한한의학방제학회지
• /
• 제12권1호
• /
• pp.255-262
• /
• 2004

The inhibitory effect of the aerial parts of Fagopyrum esculentum on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical was examined. The n-butanol fraction from the methanol extract of title plant showed stronger inhibitory effect than other fractions on DPPH radical. Two flavonoids were isolated from n-butanol fraction having the potent activity and elucidated as quercetin-3-O-${\alpha}$-L-rhamnoside and quercetin-3-O-rutinoside on the basis of spectral evidence. The $IC_{50}$ values of these compounds on DPPH radical were 6.56 ${\mu}M$ and 8.37 ${\mu}M$, respectively.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 1998년도 춘계 학술대회지
• /
• pp.41-42
• /
• 1998

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 1995년도 추계 학술대회지
• /
• pp.28-29
• /
• 1995

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 1995년도 추계 학술대회지
• /
• pp.30-31
• /
• 1995

• 양봉협회회보
• /
• 통권347호
• /
• pp.47-47
• /
• 2009

• 한국자원식물학회지
• /
• 제32권6호
• /
• pp.723-729
• /
• 2019

Fagopyrum esculentum (Buckwheat) is a globally used alternative crop that contains several useful substances with various effects; however, many of these substances (rutin, quercetin, etc.) are water insoluble. To extract these substances, alcohols is required, which is inconvenient because these solvents cause diverse problems. Many studies are underway to achieve effective extraction of these substances with water. Among of these studies, microwave assisted water extraction (MAE) has been performed extensively. In this study, we performed the extraction in various solvents and/or microwave from Fagopyrum esculentum. The analysis of the content of useful substances and the bioactivity were performed and shown to increase in MAE. Liquid chromatography-mass was performed in order to identify of the useful water-insoluble substances. Catechin, quercetin, and rutin, which are all insoluble in water, were hardly extracted with water even on heating (4.4 ppb, 3.9 ppb and 60.3 ppb, respectively). However, MAE was found to extract much more of these substances than water (1204 ppb, 110.8 ppb and 2946 ppb, respectively). Although less efficient than alcohols, MAE showed much higher efficiency than simple water extraction. These results indicate that water extraction using microwave technology is effective in cases where it is difficult to extract useful substances using water.

• 동의생리병리학회지
• /
• 제23권3호
• /
• pp.695-699
• /
• 2009

UV irradiation causes skin-aging involving coarse wrinkles, thickening, dyspigmentation, and rough skin surface. This study was carried out to develop health & functional food by using Korean red ginsneg and Fagopyrum esculentum extract mixture (RGFE) for prevention of skin wrinkles. The RGFE-treated group showed the more effective collagenase inhibition rate than the red ginseng (RG)-treated group. To investigate photo protective effects of RGFE on UV-induced damaged skin, SKH hairless male mice were orally administerd RGFE and regional treatment and irradiated with UV for up to 8 weeks. In RGFE-treated group, better skin, and less wrinkle formation were observed compared with UV group. Epidermal thickness of hairless mouse was significantly decreased in RGFE, RG, and Fagopyrum esculentum (FE) groups compared with UV group. These results demonstrate RGFE have photo-protective effects on UV-damaged hairless mouse skin.