• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.6
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• pp.1506-1512
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• 2007

In order to investigate the totale yield, the content of soluble polysaccharide and the others of FEW extract from the yeast fermentated Ganoderma lucidium by supersonic method, the yeast strain was inoculated after pretreatment and subsequently followed fermentation and supersonic extract. The main construction of the extract method is composed of the main glucose and together with the xylose, fucose, galactose and mannose. Results show that because of the generated lactic acid and ethanol, pH value of extract decreases and the safety as well as the preservation is improved. The extract yield, the total soluble polysaccharide, SOD-like activity, cytotoxic effect and growth inhibitory effect against cancer cell line are much higher in FE method than RE method, especially FEW3 extracts fermented during 24hrs. It is concluded that yeast fermentation makes the extract yield increase because of the cell disintegration, the useful ingredients of the germ body, the metabolic products, the insoluble ingredients due to the generation of ethanol, and the cell fragmentation caused by the supersonic waves vibration. Content of generated ethanol, total soluble polysaccharide and extract yield all increase during the fermentation time from 24 to 72 hours and the optimum fermentation condition is at $27^{\circ}C$ for 72 hours. The bitter taste and smell of the Ganoderma lucidium extract is diminished, fragrant-bitter taste and smell is generated so that the whole functional quality is improved.

• Korean Journal of Food Science and Technology
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• v.29 no.1
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• pp.161-166
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• 1997

Tissue thromboplastin (tissue factor), a membrane bound glycoprotein is an important initiating factor in blood coagulation cascade, which leads to the formation of thrombin by activating both factor X and IX. Activation of blood coagulation by TF is essential for blood injury, and stimulates the blood coagulation in myocardial infarction, cancer and blood coagulatory diseases. High density lipoprotein, apolipoprotein A-II were known to be biological TF inhibitors. Recently, studies on search for TF inhibitors from natural products have been active in Korea. Among the edible mushrooms screened for inhibitory activities on the TF, Lentinus edodes showed the most strong activity, followd by Agaricus bisporus and Ganoderma lucidium. And the fractionation of the above mushrooms with the chloroform ($CHCl_3$) and ethylacetate (EtOAc) was done and evaluated for the inhibitory activities on TF. In Ganoderma lucidium, $CHCl_3$ fraction and $H_2O$ layer were not active, but EtOAc fraction exhibited a strong inhibitory activity on TF and the $IC_{50}$ value was $1.07{\times}10^{-4}\;g$. In the case of Agaricus bisporus, there were no inhibitory activities on the TF in all of the fractions. $CHCl_3$ fraction and $H_2O$ layer of Lentinus edodes did not show inhibition on the TF but EtOAc fraction showed strong inhibition on the TF, and the $IC_{50}$ value was $7.70{\times}10^{-4}\;g$.

• Korean Journal of Agricultural Science
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• v.19 no.1
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• pp.33-39
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• 1992

Effects of buprofezin, a chitin synthesis inhibitor of insects, on mycelial growth, protoplast formation and reversion of Coriolus versicolor, Ganoderma applanatum and G. lucidum were investigated. The mycelial growth of C. versicolor, G, applanatum and G, lucidum was severely inhibited by buprofezin treatment, and the inhibition rate severely as the concentration of the buprofezin increased. Aerial mycelia and oidia formation of the mushrooms were increased by buprofezin treatment, but mycelial morphology was not changed. The rate of protoplast formation and reversion of G, applanatum was greatly increased by the treatment of the buprofezin, while that of G. lucidium was decreased. The rate of protoplast formation of C. versicolor was also increased when buprofezin was added to the medium, but the rate of protoplast reversion was not affected by the treatment, From the results obtained in this experiment, we found that the rate of protoplast. formation and reversion was increased by the treatment of the buprofezin in the mushrooms such as C, versicolor and G, applanatum, whose mycelial growth was fast on the medium, while that of G. lucidum, whose mycelial growth was relatively slow, was decreased by the treatment.

• Korean Journal of Food Science and Technology
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• v.29 no.1
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• pp.156-160
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• 1997

The monoamine oxidase (MAO, EC 1.4.3.4) plays a central role in the metabolism of many amines including the neurotransmitter monoamines. MAO is a flavoprotein found exclusively in the mitochondrial outer membrane, occuring in the MAO-A and MAO-B subtypes. MAO-A deaminates serotonin and noradrenaline much better than phenethylamine (PEA) or benzylamine (BA), and is preferentially inhibited by clorgyline, whereas MAO-B prefers PEA and BA as substrates and is preferentially inhibited by deprenyl. MAO inhibitors were among the first drugs used in the treatment of depression, and it is known to be the inhibition of MAO-A which is important for the antidepressant effect of MAO inhibitors. For the purpose of evaluating MAO inhibitory activities from natural resources, three kinds of edible mushrooms were screened by tracing the inhibitory activities against rat brain mitochondrial MAO-A, utilizing serotonin as a substrate and rat liver mitochondrial MAO-B utilizing benzylamine as a substrate. Among the tested mushrooms, Ganoderma lucidium and Lentinus edodes showed the weak inhibitory activities against MAO-B.

• Korean Journal of Food Science and Technology
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• v.30 no.2
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• pp.456-460
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• 1998

This research was attempted to prove suppressing effect of medicinal plants on the intestinal absorption of toxic heavy metals. In vitro study was performed by membrane filtration considering intestinal absorption conditions. From drinking water contaminated singly with 10 and 50 times level of water quality standard for heavy metals, the removal ratio of lead was $40{\sim}60%$ by Chicorium intybus, Angelica acutiloba and Ganoderma lucidium. And the removal ratio of cadmium was $20{\sim}40%$. The removal ratio of lead contaminated with both was $30{\sim}50%$ by every sample similarly, the removal ratio of cadmium was $10{\sim}30%$. The removal ratio of Angelica acutiloba was more higher than Chicorium intybus and Ganoderma lucidium. Considering the extraction conditions of samples, $70^{\circ}C$, 2 min conditions were higher than $95^{\circ}C$, 10 min conditions, the removal ratios of heavy metals were similar. And the membrane filter permeated the material under 500 mw selectively, so the final filtrate could regard as passive transport in intestinal absorption. In conclusion, this research exhibited that the medicinal plants beverages have a suppressing effect on intestinal absorption of lead and cadmium in drinking water.

• Journal of Mushroom
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• v.18 no.4
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• pp.393-397
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• 2020

Mycelium composites and leathers have versatile material properties based on their composition and manufacturing process. To prepare mycelium mat for the production of mushroom leather, several strains were mutated by gamma rays. Some mutant strains, including Lentinula edodes, Ganoderma lucidium, and Schizophyllum commune showed good hyphae growth rate and density on saw-dust media. Irradiation power (Gy), time, and height from the radiation source to the sample were examined. Based on the preliminary data obtained in this study, comprehensive research should be conducted to explore the optimal strains and culture conditions for mycelium-based leather production.