• 한국한의학연구원논문집
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• 제14권1호
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• pp.123-128
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• 2008

Glehnia littoralis (Umbelliferae), a perennial herb distributed along the coastline of northern Pacific countries, is the medicinal plant used traditionally for treatment of various diseases. This review focuses on the various pharmacological activities of Glehnia littoralis for understanding about its traditional medicinal applications, medicinal uses in the modern society, and potentials for drug development Glehnia littoralis was reported to have anti-oxidant, anti-tumor, anti-amnesic, blood circulation-promoting, immunomodulatory, anti-microbial, and allelopathic activities. However, their mechanisms remain to be clarified. Because Glehnia littoralis has been prescribed in traditional Oriental medicine as a tonic herb, Glehnia littoralis can be better than other chemical drugs and medicines which exert the equal pharmacological activities. Although the activities of Glehnia littoralis are not specifically high-potent with unique mode of action, it may turn out that it can be beneficial to exert multiple pharmacological activities. In view of low toxicity, relative cheapness, presence in the diet, and occurrence in various herbal remedies of Glehnia littoralis, it needs to be prudent to evaluate its properties and applications further.

• 한국한의학연구원논문집
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• 제14권3호
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• pp.57-63
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• 2008

Glehnia littoralis (Umbelliferae) is the medicinal plant used traditionally for treatment of immune-related diseases. Prostaglandins and nitric oxide (NO) have been implicated as important mediators in the processes of inflammation and carcinogenesis. For understanding the mechanisms for pharmacological activities of Glehnia littoralis, we evaluated the inhibitory activity of Glehnia littoralis on lipopolysaccharide (LPS)-induced prostaglandin $E_2$ ($PGE_2$) and NO production in mouse macrophage RAW264.7 cells. The results showed that the extract of Glehnia littoralis inhibited LPS- induced $PGE_2$ production effectively, but not NO. Additional study revealed that the extract of Glehnia littoralis suppressed cyclooxygenase-2 (COX-2) expression in a dose-dependent manner. Present study suggests that Glehnia littoralis may have anti-inflammatory and/or cancer chemopreventive activity through the inhibition of $PGE_2$ production by the suppression of COX-2 activity.

• 한국환경복원기술학회지
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• 제11권6호
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• pp.38-48
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• 2008

This study was conducted to evaluate the vegetation structure of community by the phytosociology method, floristic composition table on coast of South Korea. Form 2007 June until November, $2m{\times}2m$ quadrat was established in native Glehnia littoralis in order to record a dominants and coverage, and it drew the profile. It was found out that the mean temperature in the distributed areas for Glehnia littoralis population was $11^{\circ}C$ or more. The flora of the studied area in Glehnia lottoralis community of coastal dune was listed as 100 species. Glehnia lottoralis community of appearance species of Yeonggwanggun Duwori was many most by 44 species. Carex pumila, Carex Kobomugi, Imperata cylindrica var. koenigii, Ischaemum anthephehoroides and Vitex rotundifolia range all over the studied areas. And the vegetation of Glehnia littoralis community was classified into Vitex rotundifolia subcommunity, Ischaemun anthephephoroides subcommunity and Imperata cylindrica var. koenigii subcommunity. Native Glehnia littoralis was classified into preserve area, natural selection area and artificial destruction area. It must preserve native Glehnia littoralis of Goseong, Yeongdeok, Haenam it was ecological important area.

• 대한임상독성학회지
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• 제15권2호
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• pp.107-115
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• 2017

Purpose: Glehnia littoralis has been used to treat ischemic stroke, phlegm, cough, systemic paralysis, antipyretics and neuralgia. The pharmacological mechanisms of Glehnia littoralis include calcium channel block, coumarin derivatives, anticoagulation, anti-convulsive effect, as well as anti-oxidant and anti-inflammatory effects. Alpha-amanitin (${\alpha}$-amanitin) is a major toxin from extremely poisonous Amanita fungi. Oxidative stress, which may contribute to severe hepatotoxicity was induced by ${\alpha}$-amanitin. The aim of this study was to investigate whether Glehnia littoralis ethyl acetate extract (GLEA) has the protective antioxidant effects on ${\alpha}$-amanitin -induced hepatotoxicity. Methods: Human hepatoma cell line HepG2 cells were pretreated in the presence or absence of GLEA (50, 100 and $200{\mu}g/ml$) for 4 hours, then exposed to $60{\mu}mol/L$ of${\alpha}$-amanitin for an additional 4 hours. Cell viability was evaluated using the MTT method. AST, ALT, and LDH production in a culture medium and intracellular MDA, GSH, and SOD levels were determined. Results: GLEA (50, 100 and $200{\mu}g/ml$) significantly increased the relative cell viability by 7.11, 9.87, and 14.39%, respectively, and reduced the level of ALT by 10.39%, 34.27%, and 52.14%, AST by 9.89%, 15.16%, and 32.84%, as well as LDH by 15.86%, 22.98%, and 24.32% in culture medium, respectively. GLEA could also remarkably decrease the level of MDA and increase the content of GSH and SOD in the HepG2 cells. Conclusion: In the in vitro model, Glehnia littoralis was effective in limiting hepatic injury after ${\alpha}$-amanitin poisoning. Its antioxidant effect is attenuated by antidotal therapy.

• 대한임상독성학회지
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• 제16권2호
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• pp.108-115
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• 2018

Purpose: Glehnia littoralis has been reported to have several pharmacological properties but no in vivo reports describing the protective effects of this plant on${\alpha}$-amanitin-induced hepatotoxicity have been published. ${\alpha}$-Amanitin is a peptide found in several mushroom species that accounts for the majority of severe mushroom poisonings leading to severe hepatonecrosis. In our previous in vitro study, we found that ${\alpha}$-amanitin induced oxidative stress, which may contribute to its severe hepatotoxicity. The aim of this study was to investigate whether Glehnia littoralis acetate extract (GLEA) has protective antioxidant effects on ${\alpha}$-amanitin-induced hepatotoxicity in a murine model. Methods: Swiss mice (n=40 in all groups) were divided into four groups (n=10/group). Three hours after giving ${\alpha}$-amanitin (0.6 mg/kg, i.p.) to the mice, they were administered silibinin (50 mg/kg/d, i.p.) or Glehnia littoralis ethyl acetate extract (100 mg/kg/d, oral) therapies once a day for 3 days. After 72 hours of treatment, each subject was killed, cardiac blood was aspirated for hepatic aminotransferase measurement, and liver specimens were harvested to evaluate the extent of hepatonecrosis. The degree of hepatonecrosis was assessed by a pathologist blinded to the treatment group and divided into 4 categories according to the grade of hepatonecrosis. Results: GLEA significantly improved the beneficial functional parameters in ${\alpha}$-amanitin-induced hepatotoxicity. In the histopathological evaluation, the toxicity that was generated with ${\alpha}$-amanitin was significantly reduced by GLEA, showing a possible hepatoprotective effect. Conclusion: In this murine model, Glehnia littoralis was effective in limiting hepatic injury after ${\alpha}$-amanitin poisoning. Increases of aminotransferases and degrees of hepatonecrosis were attenuated by this antidotal therapy.

• 한국약용작물학회지
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• 제13권4호
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• pp.171-177
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• 2005

본 연구는 농업 자원식물의 남한 내 분포현황 및 유전자원 보존대책 확립의 일환으로 갯방풍의 자생지 생태 및 환경특성을 해안별로 조사한 바 이의 결과를 요약하면 다음과 같다. 1. 갯방풍의 분포는 갯방풍은 내륙해안과 도서지역의 해안 등 거의 전 지역의 대부분 사토에서 생육하고 있었다. 자생지는 만조선과 28.2m 거리, 해발고도 2.7m의 사구에 자생했으며, 해안별로는 동해안의 자생지가 서남해안에 비해 매우 넓고 밀도와 피도가 높았다. 2. 자생지의 혼생식물은 35여 종이었으며, 그 중 갯씀바귀, 좀보리사초, 통보리사초, 갯그령, 갯쇠보리, 띠 등의 밀도와 피도가 높았다. 3. 자생지의 연평균강수량 및 온도는 전체 평균이 각각 1250.3 mm, $12.5^{\circ}C$이었으며, 해안지역별로 보면 남해안이 연평균강수량 1427.3mm, $14.3^{\circ}C$로서 동해안의 1111.1 mm, $11.3^{\circ}C$보다 강수량이 많고 온도도 높은 편이었다. 4. 자생지의 토양의 화학적 특성은 pH가 전체평균 7.9로서 약 알카리를 띠고 있었으며, 유기물함량은 0.23%로 미약하였다. $P_2O_5$와 Ca 함량은 비교적 많았고, K은 극소량이었으며 Na이 상대적으로 적어 생육과 염분과의 상관관계가 크지 않는 것으로 보였다. 해안별로는 동해안이 서남해안과 다소의 차이를 보였다.

• 한국약용작물학회지
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• 제16권1호
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• pp.44-50
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• 2008

한약재 및 식용으로 사용되는 순비기나무, 번행초, 갯방풍의 재배 한계지역과 생리활성 성분의 효능은 다음과 같다. 1. 순비기나무, 갯방풍의 서해안 북방한계지역은 태안반도이며, 동해안 북방한계지역은 강원도 지역으로 확인되었고, 번행초의 서해안 북방한계지역은 전라남도이고 동해안 북방한계지역은 경상북도 울릉군 지역이었다. 2. 자생지가 해안 200 m 이내 모래점토와 점토흙이 혼합된 지역에서 직사광선은 받는 우점식생이 없는 것을 볼 때 재배 지역은 해안지역과 인접한 지역은 재배 가능지역으로 판단된다. 3. 생리활성효능을 RAW 264.7 세포와 HL-60 세포를 이용하여 확인한 결과 유효한 항염효능은 확인하지 못하였고, 항암활성을 검정한 결과 순비기나무, 번행초는 억제활성이 없었고, 갯방풍은 $100{\mu}g/m{\ell}$에서 60%, $200{\mu}g/m{\ell}$에서 72%의 증식억제 효과를 확인하였다.

• Natural Product Sciences
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• 제11권2호
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• pp.92-96
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• 2005

The antifungal activities of essential oils from the leaves of Glehnia littoralis, which is cultivated in Korea, were evaluated against pathogenic Trichophyton species by the broth dilution method and the disk diffusion test. Additionally, the effects of the oils together with ketoconazole were tested by the checkerboard titer test. The essential oil fraction and its main components showed significant inhibition of the tested Trichophyton fungi, with minimal inhibitor concentrations (MICs) in the range of 16-32 mg/ml. The results suggest that activities of this oil are based mainly on the contents of ${\alpha}-pinene$ (22.17%), the next prominent component of the oil fraction, while the first main components ${\beta}-pinene$ (57.83%) have relatively mild activity. The MICs of ${\alpha}-pinene$ and ${\beta}-pinene$ were 1-4 mg/ml and 4-32 mg/ml, respectively. Additionally the Glehnia oil fraction and its main components as well, exhibited significant synergism with ketoconazole against Trichophyton rubrum.

• 한국약용작물학회지
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• 제17권2호
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• pp.139-144
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• 2009

This study was conducted to evaluate the vegetation, distribution and growth pattern of Glehnia littoralis community by the DCA ordination method in eastern coast of Korea. The number of plant species growth with G. littoralis in natural habitat was found 51 taxa, and species of high frequency among 51 taxa was Ixeris repens, Calystegia soldanella, Carex kobomugi, Elymus mollis, Lathyrus japonica and Zoysia macrostachya. Indicator species of Z. macrostachya, Linaria japonica, C. soldanella, I. repens, Carex pumila, Artemisia capillaris, Oenothera biennis, L. japonica and E. mollis in G. littoralis community was differential species of syntaxonomy. C. kobomugi, Z. macrostachya, E. mollis, Cynodon dactylon and L. japonica was the distribution in dryness sand and moisture sandyloam, I. repens, C. soldanella, L. japonica and C. pumila was the distribution in dryness sand. In coastal dune, I. repens, C. soldanella, Salsola komarovi and G. littoralis combined strength of the distribution, and sand of coast was typical of the habitat.

• 대한화학회지
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• 제54권6호
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• pp.701-706
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• 2010

우리나라 동해안에 서식하는 염생식물인 갯방풍으로부터 2개의 polyacetylene 화합물인 falcarindiol(1)과 falcarinol(2), 4개의 coumarin 화합물인 bergapten(3), xanthotoxin(4), umbelliferone(5), scopoletin(6) 및 1개의 sesquiterpene 화합물인 $(5\beta,10\alpha)$-lasidiol angelate(7)가 분리되었다. 이들 화합물 중 scopoletin(6)과 $(5\beta,10\alpha)$-Lasidiol angelate(7)는 갯방풍으로부터 처음 분리되어진 것이다. 분리된 화합물의 구조결정은 $^1H$ COSY, HMQC 그리고 HMBC와 같은 2D NMR 분광학적 실험과 문헌에 보고 된 값을 비교하여 이루어졌다.