• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.2
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• pp.486-492
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• 2003

To certify the protective effect of herbal medicine on myocardial damage against oxygen free radical-induced myocardiotoxicity, cytotoxicity was measured using by MTT assay, LDH activity and thiobarbituric acid reactive substances(TBARS) assay in the presence of Guaruhaebaekbaekju-tang(GHBT) extracts or single constituents of this prescription, Myocardial toxicity was evaluated in neonatal rat myocardiocytes in cultures. In the present study, xanthine oxidase/hypoxanthine (XO/HX) resulted in a decrease in cell viability, an increase in LDH activity in culture medium and lipid peroxidation in cultured myocardial cells, In the effect of GHBT extract, it showed the prevention from the XO/HX-induced cardiotoxicity such as the decrease of LDH activity and lipid peroxidation. In the protective effect of Fructus Trichosanthis (FT) and Bulbus Allii Macrostemi (BAM), all the extracts were significantly effective in the protection of XO/HX-induced cardiotoxocity in cultured myocardial cells. From these results, they show that XO/HX is cardiotoxic in cultured myocardial cells derived from neonatal rats, and it suggests that GHBT, FT and BAM extracts are positively effective in the blocking XO/HX-induced cardiotoxicity.

• Herbal Formula Science
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• v.28 no.4
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• pp.407-428
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• 2020

Objectives : The purpose of this study is to review the effect of Guaruhaebaekbaekju-tang (GHBJ), Guaruhaebaekbanha-tang (GHBH) and Jisilhaebaekgyeji-tang (JHGJ) by summarizing Korean and English articles. Methods : The study was based on 23 papers published since 1995. Papers were searched on seven domestic electronic databases including Koreantk, NDSL, RISS, KISS, OASIS, Koreamed and Library of Korea University and three international electronic databases including PUBMED, Embase and Science Direct. Results : 1. 23 research papers were collected, including 13 in vitro studies, 6 in vivo studies, 2 case reports and 2 literature studies. 2. GHBJ, GHBH and JHGJ showed significant myocardial protective and anti-hyperlipidemic and anti-inflammatory effects. In addition, researches on anti-oxidant, thrombosis, and atherosclerosis have been conducted in various ways. 3. It can be confirmed that these studies are related to Chest and back pain (胸背痛), Insomnia by chest paralysis (胸痺不得臥), Stabbing pain that radiates from chest to back (心痛徹背), Chest numbness (心中痞), Obstruction of qi in the chest (留氣結在胸), Fullness sensation in chest (胸滿) among the terms. Conclusion : Through these results, close association was found between classic description and modern research about GHBJ, GHBH and JHGJ, and the possibility of providing objective evidence was confirmed. To improve the quality of the study, large scale studies will be required to evaluate the authority of GHBJ, GHBH and JHGJ.