• 한국균학회지
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• 제32권2호
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• pp.148-151
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• 2004

본 연구는 Phellinus linteus와 관련 담자균류 속간의 IGS I 영역의 분석에 기초한 분류체계를 정립하기 위해서 실시하였다. 실험에 사용된 Phellinus linteus 균주의 IGS I 영역은 730 bp였고 다른 담자균류의 IGS I 영역과 비교시 5' 말단부위의 $1{\sim}280\;base$까지는 각 균주들간의 보존성이 높게 나타났으며 3' 말단부위로 갈수록 보존성이 감소하다가 다시 증가하는 경향을 보였다. 담자균류의 IGS 영역에 대한 연구가 앞으로 진행된다면 ITS 영역의 비교와 더불어 새로운 계통분류지표로 이용될 수 있을 것이다.

• Journal of Positioning, Navigation, and Timing
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• 제11권2호
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• pp.83-89
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• 2022

For precise GNSS applications, the antenna phase center correction (PCC) is absolutely required. The PCC magnitude can reach the centimeter level with the antenna structure. In the present study, we first investigate the phase center offset (PCO) and phase center variation (PCO) of three different antenna models in two different reference frames, IGS08/igs08.atx and IGS14/igs14.atx. Clear L1 and L2 PCO differences were found between IGS08 and IGS14. In addition, the PCV showed characteristics that is dependent upon the signal direction (azimuth and elevation angle). The remarkable thing is that the changes of a Dorne Margolin choke-ring antenna model (AOAD/MT DOME) was very small in two reference frames. In order to analyze changes in positions according to different reference systems, GNSS data obtained from DAEJ, SUWN, and TSKB stations were processed by the precise point positioning (PPP) method. We suggest that an antenna PCO/PCV can affect the precise GNSS positioning on the order of several millimeters in two different reference frames.

• Journal of Positioning, Navigation, and Timing
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• 제5권2호
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• pp.59-66
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• 2016

The International GNSS Service (IGS) provides the IGS-Real Time Service (IGS-RTS) corrections that can be used in stand-alone positioning in real time. In this study, the positioning accuracy before and after the application of the corrections to broadcast ephemeris by applying the IGS-RTS corrections at code pseudo-range based stand-alone positioning was compared with positioning result using precise ephemeris. The analysis result on IGS-RTS corrections showed that orbit error and clock error were 0.05 m and 0.5 ns compared to precise ephemeris and accuracy improved by about 8.5% compared to the broadcast ephemeris-applied result when the IGS-RTS was applied to positioning. Furthermore, regionally dispersed five observatories were selected to analyze the effect of external environments on positioning accuracy and positioning errors according to location and time were compared as well as the number of visible satellites and position dilution of precision by observatory were analyzed to verify a correlation with positioning error.

• 한국항행학회논문지
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• 제15권6호
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• pp.953-961
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• 2011

IGS(Internation GNSS Service) 초신속궤도력에 포함된 예측궤도력은 실시간 혹은 준실시간 정밀 항법에 적합한 궤도력이다. 이 논문에서는 예측궤도력에서 발생할 수 있는 궤도 이상 발생 현황을 점검하고, NANU(Current Notice Advisories to NAVSTAR Users)와 IGS 방송궤도력(BRDC, Broadcast Ephemerides)를 이용하여 예측궤도력의 이상 검출 성능을 분석하였다. 그 결과 예측궤도력은 2010년 1년간 93회의 궤도 이상이 나타났으며, NANU를 이용할 경우 88%, NANU와 BRDC를 함께 사용할 경우 95%의 이상 검출이 가능함을 확인할 수 있었다.

• 미생물학회지
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• 제38권1호
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• pp.7-12
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• 2002

Fusarium section Liseola에 속하는 균주들의 rDNA IGS 부위를 중폭하고 여러개의 제한 효소로 처리하였다. 증폭된 IGS 부위의 길이는 F. moniliforme 12 만이 약 2.9 Kb 이고 나머지 균주들은 모두 약 2.6 Kb였다. 제한 효소 EcoRI, HincII, SalI, PstI 등은 ICS 부위를 절단하여 11균주들에서 9 haplotypes을 확인할 수 있었다. 본 연구에서의 Section Liseola에 속하는 균주들에 대한 결과에 앞서 연구되어진 Section Elegans에 속하는 균주들의 결과를 종합하여 dendrogram을 그렸을 때, IGS 부위를 종내에서와 마찬가지로 종간, section 간의 관계를 밝힐 수 있는 가능성을 제시하여 주었다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권2호
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• pp.290-296
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• 2002

Serum immunoglobulins (Igs) from Israeli carp were purified using affinity chromatography. Fish were immunized with purified mouse IgG, and the specific fish antibodies were purified from the immune serum on a mouse IgG-immobilized agarose gel. Rabbit anti-Israeli carp Igs (R $\alpha$ I. carp Igs) antibodies were produced following hyperimmunization with mouse IgG specific carp antibodies. SDS-PAGE analysis under reducing condition showed that Israeli carp Igs were composed of two $\mu$-like heavy chains with about 82 and 50 kd, respectively, and one light chain with about 25 kd. On immunoblotting analysis, however, R $\alpha$ I. carp Igs failed to react with the light chain. When both protein A and protein G-purified normal carp Ig were compared with mouse IgG-specific Israeli carp Ig, no significant structural differences among them were observed. To investigate if there is any homology between other fish Ig molecules, cross-reactivity of R $\alpha$ I. carp Igs against Ig molecules from 6 different fish sera and mouse control serum was checked on immunoblotting analysis. As a result, R $\alpha$ I. carp Igs responded to Israeli carp, common carp, and tilapia Ig molecules. In flow cytometry study, however, R $\alpha$ I. carp Igs appeared to recognize 42.0%, 35.8% and <5% of Israeli carp, common carp and tilapia $Ig^+$ head kidney cells, respectively. The result suggests the heterogeneity between receptor Igs on B-like lymphocytes and soluble Igs in serum. It is crucial to obtain pure fish Igs to produce reagent antibodies as tools for the study on their specific immune responses.

• Journal of Microbiology
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• 제39권4호
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• pp.265-272
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• 2001

Variation within the intergenic spacer(IGS) of the ribosomal DNA gene for twenty-two strains of E. oxysporum and its formae speciales was examined by PCR, couped with RELP analysis. The length of the amplified IGS region was about 2.6 kb in all strains except F.oxysporum f. sp. cucumer-inum from Korea and F. oxysporum f. sp. niveum. Those two strains were 2.5 kb long. Restriction digestion of IGS-RELP regions by Eco RI, NruI, HincII, SAlI, SmaI, BalIi, HindIII, XhoI and KpnI gave rise to nine IGS hapoltypes among all strains. Cluster analysis based on the presence of absence of comigrating restriction reagments show the two groups based on 44% genetic similarity. These results demonstrated that analysis of IGS showed some difference within and between F. oxysporum formae speciales.

• 대한수의학회지
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• 제28권2호
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• pp.365-369
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• 1988

본 연구는 감염돼지와 조직배양세포의 동결 및 파라핀 절편에서 immunogold-silver(IGS)법을 이용하여 오제스키병 바이러스를 검출코자 하였으며, 이를 위해 자돈 5두와 돼지뇌유래 섬유 아세포 및 BHK 세포를 공시하였고, IGS법과 immunoperoxidase법의 1차 항원으로는 2종의 단크론성 항체와 고도면역혈청을 사용하였다. IGS법은 양성부위에서 흑색의 미세한 과립의 침착을 볼 수 있었고, immunoperoxidase법에 비해 탁월한 효과를 얻었으며, IGS법은 조직내에 존재하는 각종 바이러스 항원을 검출하는데 이용할 수 있을 것으로 생각된다.

• ALGAE
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• 제27권2호
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• pp.75-82
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• 2012

$Arthrospira$ $platensis$ and $Arthrospira$ $maxima$ are species of cyanobacteria used in health foods, animal feed, food additives, and fine chemicals. This study conducted a comparison of the 16S rRNA gene and $cpcBA$-intergenic spacer ($cpcBA$-IGS) sequences in $Arthrospira$ strains from culture collections around the world. A cluster analysis divided the 10 $Arthrospira$ strains into two main genotypic clusters, designated I and II, where Group I contained $A.$ $platensis$ SAG 86.79, UTEX 2340, $A.$ $maxima$ KCTC AG30054, and SAG 49.88, while Group II contained $A.$ $platensis$ PCC 9108, NIES 39, NIES 46, and SAG 257.80. However, although $A.$ $platensis$ PCC 9223 belonged to Group II-2 based on its $cpcBA$-IGS sequence, this strain also belonged to Group I based on its 16S rRNA gene sequence. Phylogenetic analyses based on the 16S rRNA gene and $cpcBA$-IGS sequences showed no division between $A.$ $platensis$ and $A.$ $maxima$, plus the 16S rRNA gene and $cpcBA$-IGS sequence clusters did not indicate any well-defined geographical distribution, instead overlapping in a rather interesting way. Therefore, the current study supports some previous conclusions based on 16S rRNA gene and $cpcBA$-IGS sequences, which found that $Arthrospira$ taxa are monophyletic. However, when compared with 16S rRNA sequences, $cpcBA$-IGS sequences may be better suited to resolve close relationships and intraspecies variability.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2006년도 International Symposium on GPS/GNSS Vol.2
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• pp.267-270
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• 2006

The International GNSS Service (IGS) has managed the global GNSS network and provided the highest quality GNSS data and products, which are GPS ephemerides, clock information and Earth orientation parameter, as the standard for GNSS. An important part of its works is to provide the precise orbits of GPS satellites. GPS satellites send their orbit information (broadcast ephemerides) to users and their accuracies are approximately 1.6 meters level, but those accuracies are not sufficient for the high precise applications which require millimeters precision. The current accuracies of the IGS final orbits are within 5 centimeters level and they are used for Earth science, meteorology, space science, and they are made by the IGS analysis centers and combined by the IGS analysis center coordinator. The techniques making the products are very difficult and require the high technology. The Korea Astronomy and Space Science Institute (KASI) studies to make the IGS products. In this study, we developed our own processing strategy and made GPS ephemerides using Bernese GPS software Ver. 5.0. We used the broadcast ephemerides as the initial orbits and processed the globally distributed 150 IGS stations. The result shows about 6 to 8 centimeters in root-mean-squares related to IGS final orbits in each day during a week. We expect that this study can contribute to secure our own high technology.