• The Journal of Advanced Prosthodontics
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• 제8권3호
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• pp.172-180
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• 2016

PURPOSE. The purpose of this study was to analyze the antimicrobial, antioxidant activity and cytotoxicity of Dendropanax morbifera $L{\acute{e}}veille$ extract for assessing whether Dendropanax morbifera $L{\acute{e}}veille$ can be used for the development of natural mouthwash and denture cleaning solution. MATERIALS AND METHODS. The extract was obtained from branches of Dendropanax morbifera $L{\acute{e}}veille$. The solvent fractions were acquired by fractionating Dendropanax morbifera $L{\acute{e}}veille$ extract using n-hexane, ethyl acetate, chloroform and butanol solvent. Paper disc test was used to evaluate the antimicrobial and antifungal activity of Dendropanax morbifera $L{\acute{e}}veille$ extract and solvent fractions against Streptococcus mutans and Candida albicans. The analysis of antioxidant activity was carried out through DPPH radical scavenging assay. The cytotoxicity of Dendropanax morbifera $L{\acute{e}}veille$ extract was analyzed through MTT assay using normal human oral keratinocytes. RESULTS. Dendropanax morbifera $L{\acute{e}}veille$ extract showed antimicrobial activity against Streptococcus mutans and especially Candida albicans. The solvent fractions of Dendropanax morbifera $L{\acute{e}}veille$ showed strong antimicrobial activity against Streptococcus mutans and Candida albicans in n-hexane and butanol solvent fraction, respectively. Dendropanax morbifera $L{\acute{e}}veille$ extract also showed outstanding antioxidant activity. Butanol, ethyl acetate, and chloroform solvent fraction of Dendropanax morbifera $L{\acute{e}}veille$ tended to have increased antioxidant activity as the concentration increased. Dendropanax morbifera $L{\acute{e}}veille$ extract showed high cell survival rate in cytotoxicity test. CONCLUSION. Dendropanax morbifera $L{\acute{e}}veille$ extract turned out to have antimicrobial, antioxidant activity and cytophilicity. Based on these results, it is expected that Dendropanax morbifera $L{\acute{e}}veille$ is applicable as an ingredient for natural mouthwash and denture cleanser.

• 생명과학회지
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• 제26권11호
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• pp.1341-1344
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• 2016

보길도에서 자라고 있는 황칠나무(Dendropanax morbifera)를 구입하여, 캘러스로 유도한 후, ribosomal DNA(nrDNA)의 internal transcribed spacer (ITS) region의 염기서열을 결정하였다 보길도의 황칠나무(Dendropanax morbifera)의 ITS region의 염기서열을 분석한 결과, 총 689염기를 결정하였다. 결정된 689염기 중에서 ITS1은 222 개염기, 5.8S rDNA는 160염기, ITS2는 233염기인 것으로 판명되었다. GenBank의 BLAST 프로그램(http://www.ncbi.nlm.nih.BLAST)을 사용하여 GenBank/EMBL/DDBJ에 등록되어 있는 Dendropanax 속 33의 염기서열을 수집한 후 multiple alignment를 수행한 결과, 유사도는 99.7%(D. chevalieri)에서 92.6%(Dendropanax arboreus)로 나타났으며, 일본황칠나무(D. trifidus)와는 유사도가 99.4%로 판명되었다.

• 환경생물
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• 제28권1호
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• pp.30-39
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• 2010

남해안 고흥, 해남, 완도, 보길도지역에 분포하는 황칠나무개체군 생육지의 군락구조와 종조성, 그리고 입지환경 등을 조사하였으며, 자생지의 기상요인을 분석하여 생육가능지역을 추정하였다. 황칠나무군락은 종조성의 차이에 의해 구실잣밤나무군과 서어나무군으로 구분되었다. 상대기여도에 의한 우점도값은 전체적으로 동백나무(100.0), 붉가시나무(88.6), 구실잣밤나무(55.8), 황칠나무(41.4), 마삭줄(23.8), 사스레피나무 (19.9), 광나무(11.5) 등 상록활엽수의 분포가 높게 나타났다. 개체군 분포는 서어나무군이 흉고직경 5 cm 미만의 개체가 전체의 94.2%로 구실잣밤나무군(54.2%)에 비하여 대부분 소경목과 유목의 개체로 구성되어 있었다. 생육지의 토양환경은 약산성의 토양산도, 유기물과 토양함수량이 양호하며 배수성이 좋은 지역인 것으로 나타났다. 생육지의 분포 범위는 대체로 연평균기온이 $13^{\circ}C$ 이상, 일 최저기온이 $7.4^{\circ}C$ 이상, 온량지수(WI) $100^{\circ}C{\cdot}month$ 이상, 그리고 연강수량은 1,344 mm 내외로서 우리나라 서남부 도서와 해안에 인접한 상록활엽수림대 지역들이 해당되는 것으로 분석되었다. 황칠나무의 자연생육지에서 확인된 연평균기온 $13^{\circ}C$를 토대로 한 생육가능 등치선은 군산-정읍-광주-진주-포항-영덕을 잇는 선으로 나타났다.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1999년도 춘계학술발표대회:발표논문요지록
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• pp.5-5
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• 1999

• 한국입자에어로졸학회지
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• 제18권2호
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• pp.37-50
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• 2022

Due to the syndrome coronavirus 2 (SARS-CoV-2) pandemic, the subway passenger cabin should be continuously sterilized. However, a disinfectant such as chlorine is toxic and can lead to different issues to human health. In this paper, we introduced a novel disinfectant based on natural product (Dendropanax morbifera extract). Via ultra-high performance liquid chromatography - mass spectrometer (UHPLC-MS), different compounds from Dendropanax morbifera extract showed antivirus potentials. Antimicrobial experiments confirmed that the air-disinfectant containing Dendropanax morbifera can eliminate harmful microorganisms including Gram (-), Gram (+), and yeast within 5 mins. The as-prepared air-disinfectant also showed high antivirus activity against H1N1, HRV, and EV71. Deodorization test also indicates that the as-prepared air-disinfectant can lower the harmful gas such as ammonia and trimethylamine in the atmosphere. To evaluate the potential of air-disinfectant containing Dendropanax morbifera in practical applications, different safety tests including acute oral toxicity, acute skin irritation, and eye irritation were conducted. Results showed that the as-prepared disinfectant did not negatively affect tested animals during these safety investigations.

• 한국약용작물학회지
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• 제26권2호
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• pp.181-187
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• 2018

Background: This study aimed to investigate out the influence of drought stress on the physiological responses of Dendropanax morbifera seedlings. Methods and Results: Drought stress was induced by discontinuing water supply for 30 days. Under drought stress, photosynthetic activity was significantly reduced with decreasing soil water content (SWC), as revealed by the parameters such as Fv/Fm, maximum photosynthetic rate ($P_{N\;max}$), stomatal conductance ($g_s$), stomatal transpiration rate (E), and intercellular $CO_2$ concentration (Ci). However, water use efficiency (WUE) was increased by 2.5 times because of the decrease in $g_s$ to reduce transpiration. Particularly, E and $g_s$ were remarkably decreased when water was withheld for 21 days at 6.2% of SWC. Dendropanax morbifera leaves showed osmotic adjustment of -0.30 MPa at full turgor and -0.13 MPa at zero turgor. In contrast, the maximum bulk modulus of elasticity ($E_{max}$) did not change significantly. Thus, Dendropanax morbifera seedlings could tolerate drought stress via osmotic adjustment. Conclusions: Drought avoidance mechanisms of D. morbifera involve reduction in water loss from plants, through the control of stomatal transpiration, and reduction in cellular osmotic potential. Notably photosynthetic activity was remarkably reduced, to approximately 6% of the SWC.

• 한국가구학회지
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• 제14권2호
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• pp.13-20
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• 2003

This research is to develop kiln-drying schedules for 2.7 mm thick boards of Neolitsea sericea and Dendropanax morbifera, which grow in the southern region of Korean Peninsula. These two species are lesser-known to woodcraft industry in this country. The proper USDA drying schedules for Neolitsea sericea and Dendropanax morbifera were found to be T10-C6 and T10-E6, respectively, by using the Quick Oven-drying Method. The boards dried in a kiln with the newly-developed schedules were revealed to be free from warping and checking.

• 동의신경정신과학회지
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• 제30권4호
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• pp.341-355
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• 2019

Objectives: The purpose of this study was to check the therapeutic effect of the Dendropanax morbifera Pharmacopuncture for insomnia and cognition caused by psychosocial stress. Methods: We separated Wistar rats (older than age 10 weeks, 300 g) into normal, control, positive control (Zolpidem administered, PC), and Dendropanax morbifera Pharmacopuncture experimental groups (DPA). Psychosocial stress such as circadian rhythm change, clammy straw, predator stress, restraint stress, noise bursts, and flashing lights were applied to the control, PC, DPA groups. The Dendropanax morbifera Pharmacopuncture group was given 0.5×, 1× and 2× amount concentration of Dendropanax morbifera Pharmacopuncture, respectively. The levels of Melatonin, Serotonin, Corticosterone, GABA, BDNF and CREB were measured, and the Y-maze test, weight and blood test were performed. Results: In all of the DPA groups, the Melatonin level showed no noticeable difference. In the DPA-2x group, Serotonin increased significantly. In all the experimental groups, Corticosterone decreased significantly and GABA showed increasing tendency. The DPA-1x and DPA-2x groups showed remarkable increase in BDNF and DPA-0.5x and the DPA-2x groups showed significant increase in CREB. The DPA-2x group showed remarkable increase in the Alternation behavior category of the Y-maze test. In all of the experimental groups the weight change showed increasing tendency, whereas no noticeable differences were found among all experimental groups regarding AST, ALT, BUN, Creatinine and CBC. Conclusions: Dendropanax morbifera Pharmacopuncture increases levels of serotonin and GABA, decreases corticosterone, increases levels of BDNF and CREB, and increases the ratio of alternation behavior in the Y-maze test. Thus, I suggest that Dendropanax morbifera Pharmacopuncture has the effect of treating insomnia caused by psychosocial stress, activating the brain, and improving cognition.

• 한국자원식물학회지
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• 제31권1호
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• pp.10-15
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• 2018

황칠나무 잎과 줄기를 분리하여 각각 methanol 추출하여, 계통분획상법에 따라 dichloromethane, ethyl acetate, butanol로 분획하였다. 분획물로 실시한 TLC에서 알칼로이드 성분으로 분리되는 성분을 확인할 수 있었다. 잎과 줄기 모두 ethyl acetate분획물은 $IC_{50}$ $30{\mu}g/m{\ell}$으로 높게 측정 되었으며, ethyl acetate, dichloromethane, butanol분획물 순으로 저해 활성이 높게 나타났다. Acetyl cholinesterase inhibition assay를 실시한 결과 황칠나무 잎과 줄기 모두 dichloromethane, ethyl acetate, butanol 분획물 순으로 저해 활성이 높은 것으로 나타났으며, 가장 높은 활성을 보인 황칠나무 줄기와 잎의 dichloromethane 분획물의 알칼로이드 함량이 상대적으로 높은 것으로 보아 알칼로이드 성분에 의한 것으로 유추 할 수 있으므로, 알칼로이드 성분의 동정 및 성분구조의 규명을 통하여 AChE저해 활성을 이용한 신경관련 질환에 도움을 줄 수 있는 천연물의 개발이 가능할 것으로 사료된다.

• 동의생리병리학회지
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• 제30권5호
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• pp.301-307
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• 2016

The purpose of this study is to research the whitening effects of fermentation extract made from the Rice bran and Dendropanax(FRD) Fermentation conditions were as follows; 1) Dendropanaxand and Rice bran were blended in a ratio of 1 to 1, 2) a weight of sugar was 10% of the total weight, 3) an amount of enzyme was 0.1%, and 4) a temperature was 20℃. It has been fermented for 90 days. In order to observe the whitening effects of FRD, the author measured the cell viability and the inhibition rate of the melanin biosynthesis, the activity of tyrosinase and SOD (superoxide dismutase) in malignant melanoma, B16F10 cells. As a result, FRD significantly inhibited the cell viability of B16F10 in more than 500 ㎍/㎖. FRD significantly suppressed the generation of melanin, and that induced by α-melanocyte stimulating hormone (α-MSH) in more than 1,000 ㎍/㎖. FRD significantly decreased the activity of tyrosinase and that induced by α-melanocyte stimulating hormone (α-MSH) in more than 500 ㎍/㎖. FRD did not changed the activity of SOD in dose dependent manner. Therefore, the author considered that the fermentation extract made from a Rice bran and Dendropanax will be able to produce high value-added products, if used as a commercial. Therefore, the author considered that the fermentation extract made from a Rice bran and Dendropanax will be able to produce high value-added products, if used as a commercial.