• 제목/요약/키워드: LIFR

검색결과 11건 처리시간 0.021초

Leukemia inhibitory factor and its receptor: expression and regulation in the porcine endometrium throughout the estrous cycle and pregnancy

  • Yoo, Inkyu;Chae, Soogil;Han, Jisoo;Lee, Soohyung;Kim, Hyun Jong;Ka, Hakhyun
    • Asian-Australasian Journal of Animal Sciences
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    • 제32권2호
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    • pp.192-200
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    • 2019
  • Objective: Leukemia inhibitory factor (LIF) binds to a heterodimeric receptor composed of LIF receptor (LIFR) and glycoprotein 130 (GP130) to transmit signals into the cell. LIF plays an important role in reproduction by regulating immune response, decidualization, and implantation in several species. However, the expression of LIF and LIFR in the endometrium throughout the estrous cycle and pregnancy in pigs is not fully understood. Methods: We analyzed the expression of LIF and LIFR in the endometrium on days 0 (estrus), 3, 6, 9, 12, 15, and 18 of the estrous cycle, and days 12, 15, 30, 60, 90, and 114 of pregnancy, in conceptuses on days 12 and 15, and in chorioallantoic tissues on days 30, 60, 90, and 114 of pregnancy in pigs. We also determined the effects of estrogen and progesterone on the expression of LIF and LIFR in endometrial tissues. Results: The expression of LIF increased in the endometrium during the late diestrus phase of the estrous cycle and during mid- to late- pregnancy, while the expression of LIFR increased during early pregnancy. The expression of LIF was induced by increasing doses of estrogen, whereas the expression of LIFR was induced by increasing doses of progesterone. Conclusion: These results indicate that the expression of LIF and its receptor LIFR in the endometrium is regulated in a stage-specific manner during the estrous cycle and pregnancy, suggesting that LIF and its receptor signaling system may play critical roles in regulating endometrial function in pigs.

레이더 흡수특성이 있는 저적외선 방출 복합섬유의 제조 및 특성 연구 (Preparation and Characterization of Low Infrared Emissivity Bicomponent Fibers with Radar Absorbing Property)

  • Yu Bin;Qi Lu
    • 폴리머
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    • 제30권2호
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    • pp.124-128
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    • 2006
  • Heavy weight of the camouflage materials was always the main problem. To solve it, the low infrared emissivity fibers with the radar absorbing property (LIFR) were prepared. The low infrared emissivity fibers (LIF) were firstly melt-spun by co-extrusion of polypropylene (PP) and PP/various fillers master-batches using general conjugate spinning. The infrared emissivity of LW with AA and ZnO was decreased respectively compared with that of pure polypropylene fibers. The infrared emissivity of LIF with 15 wt% Al and 2 wt% ZnO in the sheath-part can reach 0.58. To improve LIF radar absorbing property, LIFR was prepared by filling the 50 wt% ferrite and bronze in the core-part of LIF. The radar absorbing efficacy of LIFR was good and the infrared emissivity was low. For the characterization, fiber electron intensity instrument and differential scanning calorimetry (DSC) were used for the analysis of mechanical properties, thermal and crystallization behavior of the spun-fibers. Scanning electron microscopy (SEM) was carried out to observe the particle distribution of the bicomponent fibers.

목단피에 의한 임신 저해의 분자적 기전에 대한 연구 (Mechanism Study on Inhibition of Pregnancy by Root Barks of Paeonia suffruticosa)

  • 최희정;김은영;최희진;박미주;정태욱;박성하;김소연;하기태
    • 동의생리병리학회지
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    • 제28권5호
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    • pp.530-536
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    • 2014
  • Root barks of Paeonia suffruticosa Andrews (PS) was reported as contraindicated drugs of pregnancy by many Korean medical classics. Recently, a major ingredient component of PS, paeonol was reported that has contraceptive effect on early pregnancy in rats. However, the accurate molecular mechanism is not clear. In this study, we showed that PS decreased the expression of receptor for leukemia inhibitory factor (LIFR) in human endometrial Ishikawa cells at non-toxic dose, although the expression of leukemia inhibitory factor (LIF) was increased by PS. In addition, PS inhibited the adhesion of human trophoblastic JAR cells onto Ishikawa cells. Given importance of LIF-LIFR signaling pathway in the process of embryo implantation, the decreased LIFR expression by PS will be a good explanation on the PS- or its ingredient compounds-induced contraception.

Suppression of HIF-1α by Valproic Acid Sustains Self-Renewal of Mouse Embryonic Stem Cells under Hypoxia In Vitro

  • Lee, Hyo-Jong;Kim, Kyu-Won
    • Biomolecules & Therapeutics
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    • 제20권3호
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    • pp.280-285
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    • 2012
  • The developing embryo naturally experiences relatively low oxygen conditions in vivo. Under in vitro hypoxia, mouse embryonic stem cells (mESCs) lose their self-renewal activity and display an early differentiated morphology mediated by the hypoxia-inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$). Previously, we demonstrated that histone deacetylase (HDAC) is activated by hypoxia and increases the protein stability and transcriptional activity of HIF-$1{\alpha}$ in many human cancer cells. Furthermore HDAC1 and 3 mediate the differentiation of mECSs and hematopoietic stem cells. However, the role of HDACs and their inhibitors in hypoxia-induced early differentiation of mESCs remains largely unknown. Here, we examined the effects of several histone deacetylase inhibitors (HDACIs) on the self-renewal properties of mESCs under hypoxia. Inhibition of HDAC under hypoxia effectively decreased the HIF-$1{\alpha}$ protein levels and substantially improved the expression of the LIF-specific receptor (LIFR) and phosphorylated-STAT3 in mESCs. In particular, valproic acid (VPA), a pan HDACI, showed dramatic changes in HIF-$1{\alpha}$ protein levels and LIFR protein expression levels compared to other HDACIs, including sodium butyrate (SB), trichostatin A (TSA), and apicidin (AP). Importantly, our RT-PCR data and alkaline phosphatase assays indicate that VPA helps to maintain the self-renewal activity of mESCs under hypoxia. Taken together, these results suggest that VPA may block the early differentiation of mESCs under hypoxia via the destabilization of HIF-$1{\alpha}$.

NOD(News On Demand) 데이터의 인기도와 생명주기를 이용하는 시간 윈도우에 기반한 캐시 재배치 기법 (Time Window based Cache Replacement Strategy using Popularity and Life of News-Demand Data)

  • 최태욱;박성호;김영주;정기동
    • 한국정보과학회:학술대회논문집
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    • 한국정보과학회 1998년도 가을 학술발표논문집 Vol.25 No.2 (3)
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    • pp.101-103
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    • 1998
  • 뉴스기사를 구성하는 NOD데이터는 VOD(Video on Demand) 데이터와는 달리 미디어의 종류 및 크기, 시간적인 접근 지역성, 사용자와 상호 작용성 등의 차이점을 가질 뿐만 아니라 새로운 뉴스기사가 수시로 생성되고 사용자가 인기도가 높은 기사와 최신의 뉴스기사에 더 많이 접근하는 특성을 가진다. 본 논문에서는 현재 서비스중인 전자신문의 로그파일을 분석하여 NOD 뉴스기사의 인기도가 Zipf분포와 다름을 보이고, 뉴스기사의 생명주기Lifr Cycle)에 따른 접근 확률분포 제시한다. NOD 데이터의 접근 편기성으로 인하여 데이터 캐싱을 통한 NOD 서버의 성능 향상을 기대할 수 있으나 뉴스기사의 생명주기가 짧고 접근시간대별로 사용자 접근형태가 변하는 등의 이유로 단순히 인기도만 고려한 캐싱은 빈번한 데이터 재배치 문제로 인해 높은 캐시 관리비용을 야기한다. 따라서 본 논문에서는 뉴스 기사의 접근 편기성에 나타나는 인기도(popularity)와 생명주기를 조합한 척도를 제안하고 이를 이용한 재배치를 제안한다.

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벼먹노린재(Scotinophara lurida)의 생활사와 기주식물 (Host Plants and Lifr Cycle of Rice Black Bug, Scotinophara lurida BURMEISTER(Hemiptera: Pentatomidae))

  • 이기열;안기수;강효중;박성규;김태수
    • 한국응용곤충학회지
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    • 제40권4호
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    • pp.309-313
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    • 2001
  • 벼먹노린재(Scotinophara lurida)에 대한 생활사와 기주식물에 대한 연구를 1999년부터 2001년에 걸쳐 수행하였다. 벼먹노린재의 알은 엷은 회색의 란형이다. 약충은 5령까지 발육하고, 체색은 적갈색, 흑갈색 그리고 갈색으로 체장은 1.19~7.24mm이다. 성충은 암수 모드 검은색이며 체장은 각각 9.34, 8.47mm, 월동성충의 수명은 27.2일, 산란수는 30.7개이다. 각 태별 발육기간은 알기간이 4.3일, 약충기간은 45.8일이다. 기주식물로 확인 종은 총 9종이었다.

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타액선 다형성 선종에서의 PLAG1과 CTNNB1 유전자 융합 (FUSION OF THE PLAG1 AND CTNNB1 GENES IN PLEOMORPHIC ADENOMA OF THE SALIVARY GLANDS)

  • 김재진;김은석;고승오;김효분;조남표
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제29권4호
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    • pp.206-211
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    • 2003
  • The pleomorphic adenoma is the most common neoplasm involving both the major and minor salivary glands. It is a benign, slowgrowing tumor, but local recurrences can occur. The pleomorphic adenoma gene 1 (PLAG1), which is a novel zinc finger gene, is frequently activated by reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. This experimental study was preformed to observe the translocation patterns between PLAG1 gene and the three translocation partner genes. We also have analyzed the presence of PLAG1 transcripts by RT-PCR. CTNNB1/PLAG1 gene fusion was observed in three of nine pleomorphic adnomas. However, LIFR/PLAG1 and SII/PLAG1 gene fusions were not detectable. All of three gene fusions was not detectable in one Warthin's tumor and three inflammatory salivary gland tissues. PLAG1 transcripts were expressed in all inflammatory salivary gland tissues and tumors except for three pleomorphic adenomas. Of particular one pleomorphic adenoma showing CTNNB1/PLAG1 gene fusion did not express PLAG1 transcipt. Our data indicate that gene fusion involving PLAG1 is a frequent event in pleomorphic adenoma, but correlation between gene fusion involving PLAG1 and PLAG1 transcription is not definite.

DNA Array Analysis of Changes in Gene Expression Profile in DHEA-induced PCO

  • Yu, Jeong-Min;Yoo, Seong-Jin;Kim, Do-Rim;Youm, Mi-Young, Kim, Jee-Yun;Kang, Sung-Goo
    • 한국수정란이식학회:학술대회논문집
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    • 한국수정란이식학회 2002년도 국제심포지엄
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    • pp.112-112
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    • 2002
  • Under normal conditions, women produce a single dominant follicle that participates in a single ovuation each menstrual cycle. But Polycystic ovary syndrome(PCOS) conditions, folliculogenesis does not proceed normally. This condition leads to the accumlation of large numbers of small graffian follicles in which the theca interstitial cells (TIC) produce abnormally large amounts of androgen. PCOS is probably the most common endocrine disorder, affecting women of reprodutive age with 5-10% prevalence estimate. Chronic anovulation, hyperandrogenism, hirsutism, obesity, infertility and polycystic ovaries are clinical hallmarks of women with PCOS. Its etiology remains unknown. To investigate the gene expression pattern of ovary in PCO-induced rat, we used cDNA expression analysis. Total RNA was extracted from the ovary of PCO-induced rat and reverse-transcribed in the presence of[$\alpha$$^{32}$P]-dATP Which were hybridized to Atlas$^{TM}$ Rat Toxicology 1.2 array (Clontech) representing approximately 1176 rat genes. We compared gene expression between ovary of pco-induced immature female rats and control. Differential gene expression profiles were revealed (LIFR-alpha, ADRA1A, Heat shock 90-kDa protein A, PDGFRA). Reverse transcription-polymerase chain reaction(RT-PCR) was used to validate the relative expression pattern obtained by the cDNA array. The precise relationship between the altered expression of genes and PCO is a matter of further investigation. This study was supported by Korea Science and Engineering Foundation(KOSEF)

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정.기(精.氣) 양생법(養生法)에 관한 문헌연구 (Literature Review on Spirit and Qi Regimen)

  • 백숙희;석소현;오혜경;문희자
    • 동서간호학연구지
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    • 제2권1호
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    • pp.96-106
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    • 1997
  • In Chinese medicine, the activities of removing and solving the inner cause are called the regimen in preventing the disease and the good condition of mind emphasizes us to practice ourselves the doctrine of regimen of the mind to promote the health and long life of the living body, in other words, it means we should have clean mind, less desire, less thought, less agony and widely open our emotion and will, and it also means the raising of good nature, the moral cultivation, and we can be healthy when we live by good virtus as the root of regiem. The meaning of the Qi has been expanded more and more since its origination throught the process of practice and perception of man. 1) Matter is changed to the smoke when burned, the energy of water becomes the rain in the sky and the rain raises all living creatures by making them wet. Throught these changes of circulation, men could realize the energy to be the common and original matter of forming all the creatures. 2) The direction of showing the breathing of men in and out has been expended. 3) It was widely understood as the meaning of showing vitality of men, and it was the original root of chinese medicine. 4) It was expended to be showing spirit of nature such as the sun, moon, star, sky, land, mountain and the moral spirit of men as peacefullness. By the original meaning of the word of energy expanded to the wider side of sky, land, men and things, nature, society, man and the moral spirit could get the unified basis of the matter. As the above, the word of Qi has been used in wider meaning at this time from the past. In other words, all things in the universe come into being and extinct by the chang of motion of the Qi and it is recognized to be the living activity in human body. The Qi-kong based on this energy and the motion applied to our daily life are very extensive (Lee, Hye - lung, 1997). Here are the summaries of the effect of the Qi-kong ; 1) The physical constitution is strenthened, physiological function is prosperous and we are free from various geriatric disease and psychosomatic disease not to mention the cold and indigestion. 2) It maintains the clear spirit, elevates intelligence, strengthens the spiritual power and demonstrates the potentiality at the amximum. 3) It maintain beautiful figure and clean skin never losing the charm but full of energy and vitality. 4) It keeps the balanced body never suffering from the unbalance of the bones, various and neuralgia. 5) It maintain the spiritual comfort, the natural posture in everything and real happiness (Lee, Hye-Jung, 1997 : Suh, Yong Kyu, 1989). Chinese medicine mentions the motion by Qi with Qi - kong. But the motion does not require special method in Western medicine. I t is the only way of living of our ancestor indaily life. It is maintaining the healthy lifr by training the Qi, and it is the motion of being with the nature with the open mind in breathing rather than artificially restricting our daily life, it is the motion of the body.

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