• Integrative Medicine Research
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• 제6권4호
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• pp.388-394
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• 2017

Background: The present study optimized ultrasound-assisted extraction conditions to maximize extraction yields of glycyrrhizic acid from licorice. Methods: The optimal extraction temperature ($X_1$), extraction time ($X_2$), and methanol concentration ($X_3$) were identified using response surface methodology (RSM). A central composite design (CCD) was used for experimental design and analysis of the results to obtain the optimal processing parameters. Results: Statistical analyses revealed that three variables and the quadratic of $X_1$, $X_2$, and $X_3$ had significant effects on the yields and were followed by significant interaction effects between the variables of $X_2$ and $X_3$ (p<0.01). A 3D response surface plot and contour plots derived from the mathematical models were applied to determine the optimal conditions. The optimum ultrasound-assisted extraction conditions were as follows: extraction temperature, $69^{\circ}C$; extraction time, 34?min; and methanol concentration, 57%. Under these conditions, the experimental yield of glycyrrhizic acid was 3.414%, which agreed closely with the predicted value (3.406%). Conclusion: The experimental values agreed with those predicted by RSM models, thus indicating the suitability of the model employed and the success of RSM in optimizing the extraction conditions.

• 대한암한의학회지
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• 제20권1호
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• pp.11-21
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• 2015

Objectives : The cancer incidence and cancer burden is increasing. In addition, the use of botanical agents in cancer care is increasing. This article aims to review a research strategy for botanical agents. Methods : The clinical studies of anticancer botanical agents and the papers about clinical research methodology of botanical agents were reviewed. Results : In phase I study, safety confirmation, optimal dose determination and drug interaction study are important. Most botanical agents have low toxicity and some have non-monotone dose response. Therefore, dose-response curve must be evaluated separately from the dose-toxicity curve to determine optimal dose. Although anticancer botanical agents can't shrink tumor size rapidly, they do extend survival. So, in phase II study, response should be evaluated by the survival. Conclusions : Clinical research of botanical agents in cancer is different from traditional methods and strategies. Considering the characteristics of botanical agents and experimental mechanism is necessary in conducting botanical based clinical trials.

• 대한한의학원전학회지
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• 제23권4호
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• pp.39-62
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• 2010

Background : I investigated clinical research methodology of Traditional Korean Medicine(TKM) for treatment of lung cancer with Evidence Based Medicine(EBM) approach. Methods : I conducted the internet search of National Central Library, the National Assembly Library, Korea Education & Research Information Service, Korea Institute of Science and Technology Information for Korean theses and papers, and MEDLINE for the outside articles. Conclusion : In addition to outcomes of clinical trial, abundant clinician's experience and patients' preference are also considered. Pragmatic trials and quasi-experimental trials are better than clinical trials to plan clinical design of TKM for lung cancer and appropriate endpoints includes overall survival, disease-free survival, patient reporting outcome.

• 대한한방내과학회지
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• 제44권3호
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• pp.313-353
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• 2023

Objectives: Heart failure is a chronic disease with increasing prevalence rates despite advancements in medical technology. Korean medicine utilizes herbal prescriptions to treat heart failure, but little is known about the specific herbal medicines comprising the network of herbal prescriptions for heart failure. This study proposes a novel methodology that can efficiently develop prescriptions and facilitate experimental research on heart failure by utilizing existing databases. Methods: Herbal medicine prescriptions for heart failure were identified through a PubMed search and compiled into a Google Sheet database. NetMiner 4 was used for network analysis, and the individual networks were classified according to the herbal medicine classification system to identify trends. K-HERB NETWORK was utilized to derive related prescriptions. Results: Network analysis of heart failure prescriptions and herbal medicines using NetMiner 4 produced 16 individual networks. Uhwangcheongsim-won (牛黃淸心元), Gamiondam-tang (加味溫膽湯), Bangpungtongseong-san (防風通聖散), and Bunsimgi-eum (分心氣飮) were identified as prescriptions with high similarity in the entire network. A total of 16 individual networks utilized K-HERB NETWORK to present prescriptions that were most similar to existing prescriptions. The results provide 1) an indication of existing prescriptions with potential for use to treat heart failure and 2) a basis for developing new prescriptions for heart failure treatment. Conclusion: The proposed methodology presents an efficient approach to developing new heart failure prescriptions and facilitating experimental research. This study highlights the potential of network pharmacology methodology and its possible applications in other diseases. Further studies on network pharmacology methodology are recommended.

• 대한간호학회지
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• 제37권6호
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• pp.1013-1019
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• 2007

Purpose: This study was to determine the current trend of nursing research as exploring both quantitative and qualitative methodologies, and to provide the explicit direction to improve the quality of published papers. Methods: Total of 366 articles published between 2004 and 2006 was reviewed using the criteria of analysis. Results: There was more number of quantitative studies than qualitative studies. More studies were conducted with subjects who had health problems, and studies that targeted women and elderly population have been significantly increased. In quantitative methodology, utilization of experimental and quasi-experimental designs has been increased, however descriptive study was dominant as yet. In qualitative methodology, studies using grounded theory and phenomenology were frequently published. It was noted that theoretical framework and rational for sample size were rarely presented in quantitative study. Philosophical position and the process of preparation for study, which guided the research, were not clearly described in qualitative study. Conclusion: The findings of this review suggest that published studies have been improved and diversified, however, detailed and clear evaluation tool that assesses study process and method should be developed as a way to further improve the quality of published papers.

• 대한간호학회지
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• 제36권5호
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• pp.721-731
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• 2006

Purpose: This study was done to describe the research trend of Complementary and Alternative Medicine(CAM) in Korea and internationally. Method: A Systematic review of CAM related literature published from 2000 to 2005 was done. A comprehensive search by using 34 CAM modalities as search terms to identify all articles dealing with CAM was undertaken from 4 databases: PubMed, RICHIS, KoreaMed, and Riss4U. A Total of 588 abstracts and 223 original articles were reviewed by two independent reviewers using systematic analysis guidelines. Data regarding the types of study design, characteristics of subjects and researchers, and classification of CAM modalities was recorded. The outcome measures of CAM were identified. Results: CAM research has been rapidly increasing worldwide in the last decade. In Korea, nursing(53.5%) followed by medicine(42.7%) occupied the majority of CAM research in comparison to medicine(42.9%) and disciplines in CAM(25.0%), like homeopathy, internationally. Prevailing research designs in Korea were experimental and quasi-experimental, however, randomized clinical trials were found. Internationally, survey research was dominant, with the exception of randomized clinical trials that were mostly an experimental design. Conclusion: The findings suggest that a robust research design in CAM research is needed to accumulate strong scientific evidence and to adopt nursing interventions from CAM modalities.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.81-89
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• 2023

The purpose of this study is to demonstrate the potential enhancement of the flavonoid contents from Agastache rugosa, which can be obtained as raw materials for functional products in the food medicine industry by identifying important factors for efficient preparation to save costs and time in terms of economic factors. For this reason, response surface methodology using Box-Behnken design was used to optimize the extraction conditions for the maximum yield of seven major compounds from A. rugosa. The optimum conditions were obtained with an ethanol concentration of 60.0%, a temperature of 50 ℃, and an extraction time of 33.6 min, meaning that the regression analysis fits the experimental data well. Under these conditions, the seven major compounds 1-7 had observed values of 2.169, 2.135, 0.697, 2.485, 0.105, 1.247, and 0.551%, respectively. These results show that the observed values are in good agreement with the predicted values in the regression model. This process for optimization study exhibited a basic protocol for obtaining stable ingredients from A. rugosa that are appropriate for the development of effective functional products.

• 대한약침학회지
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• 제1권1호
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• pp.87-102
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• 1997

YAKCHIM is a clinical research of korean O.M.D's in the 1960's, and it was activated by experimental research and organization of the association after 1980. YAKCHIM in Korea is a new technical therapy that used the efficacy of traditional herb and the stimulation of meridian at points by needles. In order to search the original form of YAKCHIM and the significance of development in therapeutic methodology, reger to various documents. The results were obtained as follow : 1. The form of injection in YAKCHIM is a developed form of a aspirator which trace the cupping therapy(附缸) to its origin. 2. The injection techniques that used extracts of oriental herb can be found out original methodology in Bee-acupuncture(蜂針) and snake teeth etc. that have been used in far-eastern area from long time ago. 3. YAKCHIM in Korea is a developed form of techniques which was combined with the meridian theory, in consequence, it is difference with an injection syringe which only used to injection in modern medicine.

• 대한한방소아과학회지
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• 제18권1호
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• pp.247-272
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• 2004

Objective: Organization of articles have study design, make hypothesis, sample gathering, statical analysis and feasibility of conclusion and medical articles must be continuously published and be continuously monitored in order to raise the quality of research. Main characteristics of oriental pediatrics research in korea can be summarized as the increase of the number and the advances methodology in research. The purpose of this study was to analyze the trends and evaluate the level of oriental pediatrics. Methods: The subjects were 244 articles published during the from 1986 to 2002 in 'The Journal of Korean Oriental Pediatrics' In this study, all the articles pressed in this journal were divided into three classes, Iiteratural, experimental and clinical articles and these were surveyed their chronological and thematic distribution Results: Classifying the articles in the journal of the korean oriental pediatrics, there were 103(42.2%) literatural articles, 90(36.9%) clinical articles, 51(20.9%) experimental articles. Clinical articles is gradually increasing although Iiteratural articles is decreasing. About disease in oriental pediatrics, immunoactivity was 13, rhinitis and growth was 12, allergy was 11 and diarrhea was high frequent in literatural articles, statistics of chief complaints and rhinitis was high frequent in clinical articles, immunoactivity was high frequent in experimental articles. Conclusions: As can be seen from the above results, there has been many these published using various experimental methods and relating to oriental pediatrics on the symptoms and diseases and their treatments, but unfortunately they were not enough the transmission of meaning of theses and contribution of learning, because how to do theses was not unifonn and description was not evident. It is needed more careful attention and study in the method of theses works in order to further develop the specialty of oriental pediatrics.

• Journal of Acupuncture Research
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• 제32권2호
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• pp.123-130
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• 2015

Objectives : Primary dysmenorrhea is one of the most common female gynecological diseases. Acupuncture and moxibustion therapy have been used to treat dysmenorrhea in Korea. The aim of this review was to examine the effectiveness of acupuncture and moxibustion therapy for primary dysmenorrhea as described in studies in Korea. Methods : A total of 8 databases were searched, with the search concluding February 15, 2015. These were the Oriental Medicine Advanced Searching Integrated System, DBpia, Korean Studies Information Service System, National Digital Science Library, Korean Traditional Knowledge Portal, Research Information Sharing Service, and Pubmed. Randomized controlled Trails(RCTs) comparing acupuncture or moxibustion therapy with non acupoints stimulation or medication were selected. Data abstraction and assessment of methodology was conducted by authors and disagreements were resolved by discussion. Results : 7 trials were included in this review, with a total of 308 participants. 4 trials reported on acupuncture, 1 trial reported on acupress by magnet, 1 trial reported on pharmacopuncture, and the other trial reported on moxibustion. Quality of methodology was low. 2 trials showed that experimental therapy was effective for pain relief compared to the controlled group. However, 5 trials did not show a significant difference in pain relief. Conclusions : Acupuncture and moxibustion therapy may reduce period pain, however, it is needed for well designed RCTs in Korea.