• Reproductive and Developmental Biology
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• 제35권4호
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• pp.427-434
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• 2011

Mitochondria diseases have been reported to involve structural and functional defects of complex I-V. Especially, many of these diseases are known to be related to dysfunction of mitochondrial proton-translocating NADH-ubiquinone oxidoreductase (complex I). The dysfunction of mitochondria complex I is associated with neurodegenerative disorders, such as Parkinson's disease, Huntington's disease, and Leber's hereditary optic neuropathy (LHON). Mammalian mitochondrial proton-translocating NADH-quinone oxidoreductase (complex I) is largest and consists of at least 46 different subunits. In contrast, the NDI1 gene of Saccharomyces cerevisiae is a single subunit rotenone-insensitive NADH-quinone oxidoreductase that is located on the matrix side of the inner mitochondrial membrane. The Saccharomyces cerevisiae NDI1 gene using a recombinant adeno-associated virus vector (rAAV-NDI1) was successfully expressed in AML12 mouse liver hepatocytes and the NDI1-transduced cells were able to grow in media containing rotenone. In contrast, control cells that did not receive the NDI1 gene failed to survive. The expressed Ndi1 enzyme was recognized to be localized in mitochondria by confocal immunofluorescence microscopic analyses and immunoblotting. Using digitonin-permeabilized cells, it was shown that the NADH oxidase activity of the NDI1-transduced cells was not affected by rotenone which is inhibitor of complex I, but was inhibited by antimycin A. Furthermore, these results indicate that Ndi1 can be functionally expressed in the AML12 mouse liver hepatocytes. It is conceivable that the NDI1 gene is powerful tool for gene therapy of mitochondrial diseases caused by complex I deficiency. In the future, we will attempt to functionally express the NDI1 gene in mouse embryonic stem (mES) cell.

• Biomolecules & Therapeutics
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• 제20권1호
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• pp.104-112
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• 2012

The fruit of Terminalia chebula Retzius has been used as a panacea in India and Southeast Asia but its biological activities have not been fully elucidated. Here we report anti-arthritic and analgesic effect of NDI10218, a standardized ethanol extract of Terminalia chebula, on collagen-induced arthritis and acetic acid-induced writhing model, respectively. Arthritis was induced in DBA/1J mice by immunizing bovine type II collagen and mice were treated with NDI10218 daily for 5 weeks after the onset of the disease. NDI10218 reduced the arthritis index and blocked the synovial hyperplasia in a dose-dependent manner. The serum levels of pro-inflammatory cytokines TNF-${\alpha}$, IL-6, and IL-$1{\beta}$ were significantly reduced in mice treated with NDI10218. Production of the inflammatory IL-17, but not immunosuppressive IL-10, was also inhibited in splenocytes isolated from NDI10218-treated arthritis mice. Administration of NDI10218 markedly decreased the number of T cell subpopulations in the regional lymph nodes of the arthritis mice. Finally, NDI10218 reduced the number of abdominal contractions in acetic acid-induced writhing model, suggesting an analgesic effect of this extract. Taken together, these results suggest that NDI10218 can be a new therapeutic candidate for the treatment of rheumatoid arthritis.

• 한국수정란이식학회지
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• 제25권1호
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• pp.35-42
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• 2010

Many studies propose that dysfunction of mitochondrial proton-translocating NADH-ubiquinone oxidoreductase (complex I) is associated with neurodegenerative disorders, such as Parkinson's disease and Huntington's disease. Mammalian mitochondrial proton-translocating NADH-quinone oxidoreductase (complex I) consists of at least 46 different subunits. In contrast, the NDI1 gene of Saccharomyces cerevisiae is a single subunit rotenone-insensitive NADH-quinone oxidoreductase that is located on the matrix side of the inner mitochondrial membrane. With a recombinant adeno-associated virus vector carrying the NDI1 gene (rAAV-NDI1) as the gene delivery method, we were able to attain high transduction efficiencies even in the human epithelial cervical cancer cells that are difficult to transfect by lipofection or calcium phosphate precipitation methods. Using a rAAV-NDI1, we demonstrated that the Ndi1 enzyme is successfully expressed in HeLa cells. The expressed Ndi1 enzyme was recognized to be localized in mitochondria by confocal immunofluorescence microscopic analyses and immunoblotting. Using digitonin-permeabilized cells, it was shown that the NADH oxidase activity of the NDI1-transduced HeLa cells were not affected by rotenone which is inhibitor of complex I, but was inhibited by flavone and antimycin A. The NDI1-transduced cells were able to grow in media containing rotenone. In contrast, control cells that did not receive the NDI1 gene failed to survive. In particular, in the NDI1-transduced cells, the yeast enzyme becomes integrated into the human respiratory chain. It is concluded that the NDI1 gene provides a potentially useful tool for gene therapy of mitochondrial diseases caused by complex I deficiency.

• 척추신경추나의학회지
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• 제4권2호
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• pp.1-12
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• 2009

Objectives : The purpose of this study was to investigate the relationship between pain and psychological state in female office workers with neck pain. Methods : Experimental group of 31 healthy subjects complained of neck and arm discomfort related to computer use which lasted more than 3 months in the past year and was present in the past 7 days as well as on the day of test. Outcomes were assessed by meridian-electromyography(MEMG), Neck Disability Index(NDI), Visual Analog Scale(VAS), Beck Depression Inventory(BDI), Stress Reaction Inventory(SRI), Holmes & Rahe Social Readjustment Rating Scale(SRRS). Results : The NDI score and BDI score had a correlationship(r=0.48), and the NDI score and SRI score also had a correlationship(r=0.48), significantly. The NDI score and contraction power of upper trapezius by MEMG had a correlationship significantly, but with the other muscles the NDI score didn't have correlationships. The NDI score and the SRI score were significantly higher in depression group(BDI score ${\circ}{\surd}$14). The contraction power of trapezius by MEMG was significantly lower in depression group. Conclusions : The results suggest that mental stress can be a major risk factor of neck pain in female office workers.

• 한방재활의학과학회지
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• 제23권4호
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• pp.1-7
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• 2013

Objectives The purpose of this study is to compare the effects of ShinBaro pharmacopuncture and Jungsongouhyul pharmacopuncture on whiplash injury by traffic accident. Methods This study was carried out on 30 patients who received treatment in Jaseng Hospital of Korean Medicine. 30 patients were divided ShinBaro pharmacopuncture group and Jungsongouhyul pharmacopuncture group. Visual analog scale (VAS) and neck disability index (NDI) were compared after treatment. Results 1) Both the ShinBaro group and Jungsongouhyul group showed significant improvement in the visual analog scale (VAS) and neck disability index (NDI) after 3 weeks of treatment. 2) The VAS and NDI of the ShinBaro group decreased gradually with treatment. The VAS scores showed significant improvement up to week 2, but although week 3 showed further improvement compared to week 2, the difference did not reach statistical significance. The NDI scores showed significant improvement consistantly throughout the treatment period. 3) The VAS and NDI of the Jungsongouhyul group decreased with treatment also. The VAS scores significantly improved up to week 2, but though week 3 showed further improvement in comparison to week 2, the difference did not reach statistical significance. The NDI scores showed significant improvement consistantly throughout the treatment period. 4) Although the ShinBaro group showed a swifter decline than the Jungsongouhyul group in both VAS and NDI scores, the difference was not statistically significant. Conclusions We found out that ShinBaro pharmacopuncture group and Jungsongouhyul pharmacopuncture group is effective and useful in whiplash injury by traffic accident. And, further studies will be needed.

• 대한한방내과학회지
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• 제39권4호
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• pp.732-740
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• 2018

Objective: The aim of this study was to find a correlation between the Nepean Dyspepsia Index (NDI) and electrogastrography (EGG) in clinical practice students. Methods: In total, 28 students were enrolled. NDI and EGG were measured in all students. NDI was classified as abnormal when the score was 11 or more. In EGG, standard 1 was classified as abnormal if the preprandial or postprandial percentage of normal gastric slow waves of Ch3 was less than 70% or if power ratio of Ch3 was less than 1. Standard 2 was classified as abnormal if standard 1 was met or if the percentage of normal gastric slow waves of Ch3 decreased after meals. Results: NDI and EGG had no statistically meaningful correlation when standard 1 was used, but NDI and EGG had statistically meaningful correlation when standard 2 was used. Conclusions: These findings suggest that NDI and EGG are correlated and that EGG can be used to evaluate the degree of dyspepsia.

• 한국수자원학회논문집
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• 제49권6호
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• pp.565-577
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• 2016

본 연구에서는 인위적 수공시설물을 고려하지 않는 자연상태의 가뭄해석을 위해 자연가뭄지수(NDI)를 산정하고 활용성을 평가하였다. 자연가뭄지수는 주성분 분석을 이용하여 산정하였으며, 입력자료는 3개월 누적강수량, 자연유량, 토양수분량 자료이다. 강수량은 ASOS 59개 지점 자료이고, 자연유량 및 토양수분량은 지표수문해석모형의 결과를 이용하였다. 가뭄지수의 평가기간은 1977~2012년이며, 활용성 평가를 위해 시계열 분석, 지역별 분석 및 가뭄특성인자 분석을 수행하였다. 시계열 분석결과, 자연가뭄지수는 가뭄의 시작과 끝에 대한 평균절대오차는 0.85로 가장 정확하게 나타났다. 과거 가뭄사례와 가뭄특성인자를 분석한 결과, 자연가뭄지수의 지속기간과 발생간격의 평균절대오차는 각각 1.3, 1.0으로 가뭄의 지속기간 및 발생간격을 적절히 반영하는 것을 확인하였다. 지역별 분석결과, 자연가뭄지수는 단일변량 가뭄지수의 지역별 가뭄상황을 적절히 반영하여 활용성이 높은 것으로 나타났다. 또한, 가뭄의 시작, 끝, 지속기간, 발생간격에 대한 자연가뭄지수, 표준강수지수, 표준유출지수, 표준토양수분지수의 순위를 분석한 결과 자연가뭄지수가 가장 높은 순위로 산정되었다. 자연가뭄지수는 기존 단일변량 가뭄지수의 상이한 가뭄상황을 종합적으로 반영하며, 가뭄의 특성을 정량적으로 제시한다는 점에서 활용성이 높다고 판단된다.

• Clinical and Experimental Pediatrics
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• 제48권6호
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• pp.669-674
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• 2005

신성 요붕증은 항이뇨호르몬의 정상적 분비에도 불구하고, 신장의 집합관의 항이뇨호르몬에 대한 반응이 저하되어 요농축능에 이상이 초래되는 질환이다. 특히 선천성 신성 요붕증은 대게 반성 열성 유전 양식을 따르는 유전 질환으로 매우 드물어 소아에서는 간헐적 보고만 있어 왔다. 어린 소아에서는 증상이 비특이적일 수 있고, 임상적 진단도 쉽지 않은데, 최근에는 항이뇨 호르몬 수용체 유전자의 돌연변이들이 확인되어 유전자 검사로 확진이 가능하게 되었다. 기존의 보고들은 선천성 신성 요붕증이 진단된 환아들에 대한 이뇨제나 비스테로이드성 항염증제 등을 포함한 치료가 이루어진 증례보고이었으나 이들의 치료 후 장기적 추적 결과 보고가 극히 드물며, 이들 약제에 의한 치료 효과는 낮은 것으로 알려져 있다. 저자들은 극심한 이뇨로 인한 이차적 요로기관의 변형이 초래되었던 8세 소아에서 환아와 엄마의 말초 혈액 유전자 분석 검사상 Xq28 염색체 부위의 AVPR2 유전자의 돌연변이가 확인되었고 hydrochlorothiazide, indomethacin 및 amiloride 병합 치료 후 배뇨량은 하루 12리터에서 4리터로 감소하였고, 성장 발육도 정상이었으나 더 이상의 호전이 없고 일상 생활에 불편함이 지속되어 보조적 방광루 형성술을 시행받은 후, 증상 호전 및 심리적 안정을 얻었던 심한 선천성 신성 요붕증 1례의 5년간의 추적 관찰 결과를 보고하는 바이다.

• 한방재활의학과학회지
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• 제24권2호
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• pp.131-140
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• 2014

Objectives This study was aimed at comparing clinical effectiveness among trigger point treatment, Self-exercise treatment, trigger point - self-exercise cooperative treatment for neck pain caused by traffic accidents. Methods 63 patients with neck pain by traffic accidents were divided into 3 groups. Different types of treatments were carried out for each groups ; group A with trigger point, group B with self-exercise, group C with trigger point and self-exercise cooperative treatment. 3 groups were also treated with general acupuncture and herb-medicine treatment. Pain threshold, visual analogue scale (VAS), and neck disability index (NDI) were checked on a daily basis. SPSS ver. 18.0 for Windows was used for analysis of data. The effectiveness of treatment of each groups were verified by using paired test and one-way repeated-measures ANOVA test, and the comparison of VAS, NDI and pain threshold were demonstrated by independent samples t-test. Results 3 groups all showed significant difference in pain threshold, VAS and NDI after 8 days later. Group C showed more improvement in pain threshold, VAS and NDI than shown in group A and B. In the first period (from pre-treatment to 4th day treatment) group C showed significant difference in NDI and pain threshold but not in VAS as compared to group A and B. In the second period (from 4th treatment to 8th day treatment), Group C showed significant difference in pain threshold, VAS and NDI as compared to group A and B. Conclusions Trigger point - self-exercise cooperative treatment is proved to be more helpful to improve the unstability and reduce neck pain than trigger point treatment or self-exercise treatment only, therefore eventually leading to better satisfaction for patients with neck pain.

• 대한통합의학회지
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• 제7권4호
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• pp.151-159
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• 2019

Purpose : This study aimed to evaluate the effects of sitting questionmark exercise (SQE) and brugger's relief exercise (BRE) on pain, ROM, proprioception, NDI in 60 Patients with Chronic Cervical Pain (CCP). Methods : In this study the VAS and NDI were used to investigate changes in pain and disability with SQE and BRE. The pre and post intervention intra group differences were analysed with a paired t-test for mean values, and the inter group differences were analyzed with an independent t-test for mean values. Results : The pain of both of groups was lowered with statistical significance. Pain of SQE group is lower than that of BRE group with statistical significance. ROM in both groups was improved, but there is no significant difference between two groups. NDI in both of groups were significantly decreased after the intervention, but there was no significant difference between groups. there was NDI ratio (%) significant difference between each groups. Conclusion : SQE intervention may be considered a more effective clinical approach for reducing pain and restoring proprioception in patients with CCP.