• 동의생리병리학회지
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• 제29권5호
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• pp.403-408
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• 2015

Traditionally, 5 kinds of fruits with "ja(子)" in their name, including Rubus coreanus, Schisandra chinensis, Lycinum chineuse, Torilidis fructus, and Cuscuta seed, collectively called Oja(五子), are long known to enhance stamina. In the present study, we replaced tosaja with gyeolmyeongja(Cassiae semen ) and examined the effects of extracts from these fruits on andropause. This study investigated the antioxidant effect and testosterone synthesis of Oja water extract on Leydig TM3 cells. To investigate whether hydrogen peroxide induces oxidative stress in Leydig cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay, nitric oxide assay, and testosterone assay were performed on mouse Leydig TM3 cells. The results were obtained as follows: Leydig TM3 cells viability was assessed by a modified MTT assay, and the protection effect of Oja water extract against hydrogen peroxide-induced cell oxidative stress were examined by mitochondrial activity. Oja water extract could efficiently protect cytotoxicity induced by H₂O₂. Oja water extract promoted testosterone synthesis. These results suggest that Oja water extract has protective roles and promotes steroidogenesis in Leydig cells through its anti-oxidant action.

• 동의신경정신과학회지
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• 제19권3호
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• pp.55-68
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• 2008

Background: Microglia produces a barrage of factors (IL-l, TNF-$\alpha$, NO, superoxide) that are toxic to neurons and playa major role in the cellular immune response associated with the pathology of Alzheimer's disease(AD). OJaJiHwangEumJa(OJJHEJ) has been usually used for the treatment of senile disorders. For enhancing efficacy and convenience, the change of the drug delivery device of oriental herbal medicine is required. Objective: This experiment was designed to investigate the effect of the OJJHEJ hot water extract & ultra-fine powder on proinflammatory cytokine of microglia and memory deficit model. Method: The effects of the OJJHEJ hot water extract on production of IL-1$\beta$, IL-6, TNF-$\alpha$, in BV2 microglial cell line treated by lipopolysacchaide(LPS) were investigated. The effects of the OJJHEJ hot water extract & ultra-fine powder on the behavior of the memory deficit mice induced by scopolamine and AChE in serum of the memory deficit mice induced by scopolamine were investigated. Results: 1. The OJJHEJ hot water extract suppressed the production of IL-1$\beta$, IL-6, TNF-$\alpha$ in BV2 microglial cell line and the production of IL-6 was suppressed significantly. 2. The OJJHEJ hot water extract & ultra-fine powder decreased AChE significantly in the serum of the memory deficit mice induced by scopolamine. 3. The OJJHEJ hot water extract & ultra-fine powder groups showed significantly inhibitory effect on the scopolamine-induced impairment of memory in the experiment of Morris water maze. Conclusions: This experiment shows that the OJJHEJ hot water extract & ultra-fine powder might be effective for the prevention and treatment of memory impairment diseases. Investigation into the clinical use of the OJJHEJ hot water extract & ultra-fine powder for Alzheimer's disease is suggested for future research.

• Journal of the Korean Data and Information Science Society
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• 제7권1호
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• pp.113-118
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• 1996

주성분 분석은 자료압축, 특징추출, 통신이론, 패턴인식 그리고 화상처리등의 컴퓨터 공학분야에서 중요하게 사용되고 있다. Oja(1982,1989,1992)는 확률적 경사 강하법(SGA:Stochastic Gradient Ascent)을 이용한 제한된 헵규칙을 제안하여 주성분 분석에 사용하였다. 그러나, 이 규칙은 이상치에 민감하므로 이상치의 영향을 줄이기 위해, Xu & Yuille(1995)는 통계물리 방법을 이용한 로버스트 에너지함수를 생성하여 로버스트 주성분 분석방법을 제안하였다. 또한 Devlin et.al(1981)은 M-추정량을 이용하여 주성분 분석을 하였다. 본 논문에서는 Oja(1992)의 규칙과 Xu & Yuille(1995)의 로버스트 에너지함수를 이용하여 신경망을 구성하였다. 그리고, Devlin et.al(1981)이 제안한 시뮬레이션조건하에서 실험을 하였다. 실험한 결과와 Devlin et.al(1981)의 결과를 비교, 분석함으로써, 신경망의 성능을 확인하고자 한다.