• Korean Journal of Environmental Agriculture
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• v.20 no.2
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• pp.99-107
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• 2001

PCBs(Polychlorinated biphenyls) in air and plants were measured every two weeks at Hankyong University located in Ansung, Kyoung ki province from July to November in 1999. The percentage of tri-CBs(PCB 28) and tetra-CBs(PCB 52) is 49% of the total PCBs in air, which shows these two components are the major congeners. A correlation coefficient(r) of PCB congeners detected in two plants is 0.83 (p<0.01), indicating that the plants can be used as a passive sampler to indirectly estimate the pollution levels of residual PCBs in the environmental even if they have different physicochemical properties. The average ratio of PCB 110/PCB 52 is higher in plants as 0.5, 2.9 and 2.5 respectively in the air and mulberry leaves as well as scallions, which comes from the higher lipophilicity of PCB 110 rather than the ratio of PCB 110/PCB 52 in the air.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.1
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• pp.172-180
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• 2002

Polychlorinated biphenyls(PCBs) are large scale industrial chemicals which are using in diverse applications. The goal of this study was to determine if exposure to 2,2',5,5'-tetrachlorobiphenyl (PCB 52) leads to an increase in the production of active oxidants, and subsequently promotes apoptosis of neuronal SK-N-MC cells. Reactive oxygen species (ROS) formation was examined in SK-N-MC cells after treatment of PCB 52 by concentrations and incubation times, respectively. It showed that the rate of ROS production in the cells was increased in a does-dependent manner to 45 min, followed by a return towards control levels after 120 min treatment. We also examined the association of PCB-induced apoptosis with the modulation of biomakers of oxidative damage to lipids (malondialdehyde [MDA]) in SK-N-MC cells. Increased MDA was observed in a dose-dependent manner in groups treated with 10, 15, and 20 figJ me of PCB 52 for 24 h. After treatment of PCB 52, the cells did not show any significant change in the rate of Cu/Zn-superoxide dismutase (Cu/Zn-SOD) activity. Whereas, the cells had a two-fold greater rate of change in catalase activity at 20 ㎍/㎖ of PCB 52 for 24 h when compared to control group. Korean Ginseng is one of the most important crude drugs which has been used as a traditional Oriental medicine. We next investigated protective effect of extracts of ginseng on cytotoxicity induced by PCB 52 in SK-N-MC cells. Pretreatment of SK-N-MC cells with 25-200 μg/ml of ginseng were reduced cell death in a dose-dependent manner in PCB 52-treated cells. To examine the sensitivity of beta-catenin to ginseng, the protective effect of a range of ginseng concentrations was examined in SK-N-MC cells treated with PCB 52. The result demonstrated that ginseng efficiently blocked PCB 52 inducible beta-catenin proteolysis in a concentration dependent manner. The ROS formation was also measured in the presences of extract of ginseng and superoxide dismutase (inhibitor of oxygen free radical production). The both SOD (400 U/ml) and ginseng (200 μg/ml) significantly inhibited RDS generation in PCB 52-treated group.

• Analytical Science and Technology
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• v.23 no.3
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• pp.312-321
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• 2010

Seven indicator PCBs (IUPAC nos 28, 52, 101, 118, 138, 153, 180 congeners) concentrations were measured in food samples including cereal (polished rice), meats, eggs, milk and dairy products, and fisheries and products as representation for the general Korean populations during 2006-2007, and was analyzed using isotopic dilution method. Fishes had the highest average level as 39.8 ng/g, 1.4 ng/g for milk and dairy products, and 0.9 ng/g for meats. The hairtail out of fishes was contaminated at the level of 15.4 ng/g, 5.4 ng/g for pacific mackerel and spanish mackerel, and 4.5 ng/g for yellow croaker. The ratio for indicator PCBs in overall food was contributed as follows; 35.8% for PCB-153, 16.2% for PCB-138, 16.1% for PCB-101, 13.4% for PCB-118, 8.8% for PCB-180, 6.9% for PCB-52, and 2.9% for PCB-28. The hexa-CBs including PCB-153 and 138 were more predominated, and the next was penta-CBs including PCB-101 and 118 in food. For estimated daily intake (EDI) in average and $95^{th}$ percentile, fishes and products out of overall food were taken to represent over 50%. However, it was estimated that there was no adverse health effect for Korean.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2003.05a
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• pp.52-52
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• 2003

• Toxicological Research
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• v.28 no.2
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• pp.107-112
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• 2012

Polychlorinated biphenyls (PCBs) are persistent and bioaccumulative environmental pollutants. Recently, it is suggested that neurotoxic effects such as motor dysfunction and impairment in memory and learning have been associated with PCB exposure. However, structure relationship of PCB congeners with neurotoxic effects remains unknown. Since PKC signaling pathway is implicated in the modulation of motor behavior as well as learning and memory and the role of PKC are subspecies-specific, we attempted to study the effects of structurally distinct PCBs on the total PKC activity as well as subspecies of PKC in cerebellar granule cell culture model. Cells were exposed to 0, 25 and 50 ${\mu}M$ of PCB-126, PCB-169, PCB-114, PCB-157, PCB-52 and PCB-4 for 15 min. Cells were subsequently analyzed by [$^3H$] phorbol ester binding assay or immunoblotted against PKC-${\alpha}$ and -${\varepsilon}$ monoclonal antibodies. While non-dioxin-like-PCB (PCB-52 and PCB-4) induced a translocation of PKC-${\alpha}$ and -${\varepsilon}$ from cytosol to membrane fraction, dioxin-like PCBs (PCB-126, -169, -114, -157) had no effects. [$^3H$] Phorbol ester binding assay also revealed structure-dependent increase similar to translocation of PKC isozymes. While PCB-4 induced translocation of PKC-${\alpha}$ and -${\varepsilon}$ was inhibited by ROS inhibitor, the pattern of translocation was not affected in presence of AhR inhibitor. It is suggested that PCB-4-induced PKC activity may not be mediated via AhR-dependent pathway. Taken together, our findings suggest that chlorination of ortho-position in PCB may be a critical structural moiety associated with neurotoxic effects, which may be preferentially mediated via non-AhR-dependent pathway. Therefore, the present study may contribute to understanding the neurotoxic mechanism of PCBs as well as providing a basis for establishing a better neurotoxic assessment.

• Journal of Environmental Health Sciences
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• v.9 no.1
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• pp.85-88
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• 1983

캄덴 카운티 청사에 있는 transformer에 화재가 난 후 건물내에 있는 60명의 혈야에서 PCB를 측정하였다. 화재가 발생한 건물내의 공기에서는 거의 검출되지 않았으나 transformer가 있는 방의 Swab Sample에서는 $52.6 \mug/cm^2$가 검출되었다. 건물을 재사용하기 전에 환기를 최대로 시킨후 다시 Swab Sample을 취했을때 12 Sample중 10주에서 PCB가 검출되지 않았다. 또한 혈야에서 PCB수준은 미검출에서 16ppb였으나 일반인에서도 69.2ppb까지 검출된것과 그밖에 17.6ppb까지 검출된 것으로 보아 화재후 PCB가 혈야중에서 증가했다는 증거는 없다고 본다. 이와 같이 혈청내에서 PCB가 증가하지 않은것은 화재후 즉시 청소와 환기를 실시한 결과로 본다.

• Analytical Science and Technology
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• v.16 no.2
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• pp.166-173
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• 2003

To determine levels of PCBs in food, beef, pork, chicken, egg, mackerel, yellow croaker, anchovy, common squid and little neck clam were chosen and collected at markets in Seoul, Busan and Kwangju. Among 209 PCB congeners, 7 congeners (#28, #52, #101, #118, #138, #153 and #180) were selected as target compounds that were known as indicator congeners. Samples were homogenized, treated in alkali solution for 1 hour, and extracted with organic solvents. After extraction, extracts were cleaned up by sulfuric acid, purified on silica gel column chromatography, analyzed by dual column-HRGC/ECD and then confirmed by HRGC/MSD. As results, PCBs were detected in fish samples ranged from 0.0002 to 0.001 mg/kg. Both PCB #101 and PCB #118 were the major contributors among 7 congeners.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.52.2-52
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• 2001

We propose a new optimization method to improve the productivity of dispenser machines in PCB assembly lines. The optimization problem for multi-nozzle dispensers is formulated as a variant TSP. A genetic algorithm is applied to the problem to get a near-optimal solution. Chromosome and some operator are newly defined to implement the genetic algorithm for multi-nozzle dispensers. Simulation results are then presented to verify the usefulness of the method.

• Journal of Environmental Health Sciences
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• v.29 no.2
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• pp.29-37
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• 2003

The purpose of this study was to identify the distribution of non-ortho and mono-ortho PCB congeners and homologues in adipose tissues and sera of women with breast cancer. The collected samples were 25 adipose tissues and 33 sera from women with breast cancer. The samples from the control group were 49 adipose tissues and 52 sera. The levels of three non-ortho and eight mono-ortho PCBs identified in adipose tissue and serum samples were determined by GC/MSD and GC/ECD analyses. Non-ortho and mono-ortho PCB congeners were more dominant in the control group than in the case group for serum samples. The Tetra-PCB and the Hexa-, Hepta-PCB were more dominant in tale and control groups, respectively. The level of PCB homologues in normal women was similar to that of the normal human milk samples. However, the levels of PCB homologues from breast cancer patients were almost same the level of sample from environment. As a result of this study, it is suggested that breast cancer could be related to environmental factors such as PCB level in stack gas and soil sample. More extended research should be to verify this result.

• Analytical Science and Technology
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• v.25 no.6
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• pp.421-428
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• 2012

In this study, PCDD/PCDFs, PAHs and PCBs in wastes from metal, plastic and wastewater treatment facilities were analyzed. The concentrations of PCDD/PCDFs ranged from 7.37~432.20 ng-TEQ/kg in fly ash, 0.51~855.01 ng-TEQ/kg in incinerated ash and 0.37~385.81 ng-TEQ/kg in dust. Dioxin content was lower, compared to data in foreign countries. PAHs concentration was in the range of 0.0075~2.9225 mg/kg for process sludge and 0.0035~1.6716 mg/kg for wastewater sludge, which satisfied all of the two standards (Nap, Ant, B(a)F:4/0.8, Phen, B(a)A:5/1, Flt:10/2.5, B(a)P:4.5/0.9) of the Marine Environment Management Act. PAHs concentration in process sludge and wastewater sludge were slightly lower than those abroad. According to the analysis of seven types of PCBs (in comparison with the first standard, 0.15 mg/kg), concentration was found in the range of 0.0~0.65 mg/kg, while PCB-52, PCB-101, PCB-138, PCB-153 and PCB-180 isomers were detected in excessive value in some machine oil and hydraulic fluid.