• The Korean Journal of Physiology and Pharmacology
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• v.21 no.2
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• pp.267-273
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• 2017

The p53-inducible gene 3 (PIG3), initially identified as a gene downstream of p53, plays an important role in the apoptotic process triggered by p53-mediated reactive oxygen species (ROS) production. Recently, several studies have suggested that PIG3 may play a role in various types of cancer. However, the functional significance of PIG3 in cancer remains unclear. Here, we found that PIG3 was highly expressed in human colon cancer cell lines compared to normal colon-derived fibroblasts. Therefore, we attempted to elucidate the functional role of PIG3 in colon cancer. PIG3 overexpression increases the colony formation, migration and invasion ability of HCT116 colon cancer cells. Conversely, these tumorigenic abilities were significantly decreased in in vitro studies with PIG3 knockdown HCT116 cells. PIG3 knockdown also attenuated the growth of mouse xenograft tumors. These results demonstrate that PIG3 is associated with the tumorigenic potential of cancer cells, both in vitro and in vivo, and could play a key oncogenic role in colon cancer.

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.4
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• pp.274-278
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• 2020

Pig slurry (PS) is the most applicable recycling option as an alternative organic fertilizer. The application of pig slurry has the risk of air pollution via atmospheric ammonia (NH3) and nitrous oxide (N2O) emission. The zeolite has a porous structure that can accommodate a wide variety of cations, thus utilizing for the potential additive of deodorization and gas adsorption. This study aimed to investigate the possible roles of zeolite in mitigating NH3 and N2O emission from the pig slurry applied to the maize cropping. The experiment was composed of three treatments: 1) non-N fertilized control, 2) pig slurry (PS) and 3) pig slurry mixed with natural zeolite (PZ). Both of NH3 and N2O emission from applied pig slurry highly increased by more than 3-fold compared to non-N fertilized control. The NH3 emission from the pig slurry was dominant during early 14 days after application and 20.1% of reduction by zeolite application was estimated in this period. Total NH3 emission through whole period of measurement was 0.31, 1.33, and 1.14 kg ha-1. Nitrous oxide emission in the plot applied with pig slurry was also reduced by zeolite treatment by 16.3%. Significant increases in forage and ear yield, as well as nutrient values were obtained by pig slurry application, while no significant effects of zeolite were observed. These results indicate that the application of zeolite and pig slurry efficiently reduces the emission of ammonia and nitrous oxide without negative effects on maize crop production.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.16 no.1
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• pp.36-43
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• 2006

The principal aim of this field study was to determine the concentrations and emissions of gaseous contaminants such as ammonia and hydrogen sulfide in the different types of pig buildings in Korea and allow objective comparison between Korea and the other countries in terms of pig housing types. This field study was performed from May to June and from September to October in 2002. Pig buildings investigated in this research were selected in terms of three criteria; manure removal system, ventilation mode and growth stage of pig. Measurements of concentration and emission of ammonia and hydrogen sulfide in the pig buildings were done in 5 housing types and the visited farms were 15 sites per each housing type. Concentrations of ammonia and hydrogen sulfide were measured at three locations of the central alley in the pig building and emission rates of them were estimated by multiplying the average concentration($mg/m^3$) measured near the air outlet by the mean ventilation rate($m^3/h$) and expressed either per pig of liveweight 75kg(mg/h/pig) or per area($mg/h/m^2$). Concentrations of ammonia and hydrogen sulfide in the pig buildings were averaged to 7.5 ppm and 286.5 ppb and ranged from 0.8 to 21.4 ppm and from 45.8 to 1,235 ppb, respectively. The highest concentrations of ammonia and hydrogen sulfide were found in the mechanically ventilated buildings with slats; 12.1 ppm and 612.8 ppb, while the lowest concentrations of ammonia and hydrogen sulfide were found in the pig buildings with deep-litter bed system(2.2 ppm) and the naturally ventilated pig buildings with manure removal system by scraper(115.2 ppb), respectively(p<0.05). All the pig buildings were investigated not to exceed the threshold limit values(TLVs) of ammonia(25 ppm) and hydrogen sulfide(10 ppm). The mean emissions of ammonia and hydrogen sulfide per pig(75kg in terms of liveweight) and area($m^2$) from pig buildings were 250.2 mg/h/pig and 37.8 mg/h/pig and $336.3mg/h/m^2$ and $50.9mg/h/m^2$, respectively. The pig buildings with deep-litter bed system showed the lowest emissions of ammonia and hydrogen sulfide(p<0.05). However, the emissions of ammonia and hydrogen sulfide from the other pig buildings were not significantly different(p>0.05). Concentrations and emissions of ammonia and hydrogen sulfide were relatively higher in the pig buildings managed with deep-pit manure system with slats and mechanical ventilation mode than the different pig housing types. In order to prevent pig farm workers from adverse health effect caused by exposure to ammonia and hydrogen sulfide in pig buildings, they should wear the respirators during shift and be educated sustainably for the guideline related to occupational safety.

• Journal of Environmental Science International
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• v.31 no.1
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• pp.99-102
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• 2022

The aim of this study was to investigate the effect of manure additives mixed with probiotics and zeolite on harmful gas production generated by pig slurry. A total of 180 crossbred pigs ([Yorkshire × Land race] × Duroc, live weight 70±3.21 kg) were allotted to a completely randomized design with 3 treatments and 3 replications (20 heads per replicate). The treatments consisted of 0% (control), 0.05% (T1), or 1% (T2) of manure additives mixed with probiotics and zeolite. Manure additives were added weekly to pig slurry pits (2 m × 4.5 m × 1.2 m) on a volumetric basis. For ammonia measured at both 10 cm and 90 cm above the pig slurry pit, a statistical significance (p<0.05) was found in probiotics and zeolite-treated manure additives at weeks 1 - 3, except for week 0. In addition, hydrogen sulfide levels measured at 10 cm above the pig slurry pit were not affected by the manure additive at weeks 0 and 1, but showed a significant statistical difference at weeks 2 and 3 (p<0.05). Therefore, supplementing pig slurry with 0.05% and 0.1% manure additives mixed with probiotics and zeolite was found to be effective in reducing environmental pollution in pig facilities.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.1
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• pp.47-53
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• 2022

The objective of this study was to verify the effect of pig slurry application with acidification and biochar on feed value, nitrogen use efficiency (NUE) of maize forage, and ammonia (NH₃) emission. The four treatments were applied: 1) non-pig slurry (only water as a control, C), 2) only pig slurry application (P), 3) acidified pig slurry application (AP), 4) acidified pig slurry application with biochar (APB). The pig slurry and biochar were applied at a rate of 150 kg N ha^-1 and 300 kg ha^-1, respectively. The AP and APB treatments enhanced all feed values compared to C and P treatments. The NUE for plant N was significantly increased 92.1% by AP and APB treatment, respectively, compared to the P treatment. On the other hand, feed values were not significantly different between AP and APB treatments. The acidification treatment with/without biochar significantly mitigated NH₃ emission compared to the P treatment. The cumulative NH₃ emission throughout the period of measurement decreased by 71.4% and 74.8% in the AP and APB treatments. Also, APB treatment reduced ammonia emission by 11.9% compared to AP treatment. The present study clearly showed that acidification and biochar can reduce ammonia emission from pig slurry application, and pig slurry application with acidification and biochar exhibited potential effects in feed value, NUE, and reducing N losses from pig slurry application through reduction of NH₃ emission.

• Journal of Environmental Science International
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• v.33 no.2
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• pp.187-191
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• 2024

This study aimed to investigate the effect of treating environmental purification insect larvae to pig manure on crude ash contents and ammonia production. The experiment set up consisted go two groups: 1 kg of each 3^rd instar TM (Tenebrio molitor) and 3^rd instar PBS (Protaetia brevitarsis seulensis) larvae in Experiment 1 or 3^rd and 4^th instar of HI (Hermetia illucens L.) larvae in Experiment 2 were treated with 5 kg of pig manure. In Experiment 1, the crude ash content was higher in TM larvae-treated pig manure at days 0 and 5 (p>0.05), but was similar to that in PBS larvae-treated pig manure over (p>0.05). Ammonia production was observed at day 0 of TM and PBS larvae-treated pig manure (p<0.05), but did not occur thereafter. For Experiment 2, there was significant difference in crude ash content of 3^th and 4^th instar HI larvae-treated pig manure on day 15. Additionally, ammonia production was found in 3^th and 4^th instar HI larvae-treated pig manure at days 0 and 5, but did not continue over time. In conclusion, treating TM, PBS and HI to pig manure changed the crude ash contents and reduced ammonia through the ability to decompose pig manure. Thus, environmental impact can be minimized using environmental purification insect larvae.

• Journal of Animal Environmental Science
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• v.5 no.2
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• pp.93-100
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• 1999

Pig's original image data was transformed to a binary image, an image excluding head and tail portion from the whole binary image, and a projected image associated with pig's height. Then the length of body, width of shoulder, and area of pig were calculated and the relationships among the above characteristics and pig's weight were analyzed. The results obtained from this study were as follows: 1. Whole binary image data was considered to be improper to determine the pig's weight because the movement of pig's head and tail portion affected the image data. 2. Binary image data excluding head and tail portion from the whole binary image showed a better estimation of the pig's weight than the whole binary image. 3. Pig's should width was analyzed to be improper factor to determine the pig's weight. 4. The projected image associated with pig's height showed the highest correlation between the pig's area of the image and pig's weight(R2=0.9965). From this research the projected image associated with pig's height, which is excluding head and tail portion from the whole body of pig's image, was considered to be the prime factor to measure the pig's weight by the noncontact measurement.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.3
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• pp.145-149
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• 2018

The objective of this study was to determine the effect of injection application of pig slurry on ammonia ($NH_3$) and nitrous oxide ($N_2O$) emission from timothy (Phleum pretense L.) sward. The three treatments were applied: 1) only water as a control, 2) pig slurry application by broadcasting, 3) pig slurry application by injection. The pig slurry was applied at a rate of $200kg\;N\;ha^{-1}$. Total $NH_3$ and $N_2O$ emission, expressed as a cumulative amount throughout the measurement time (40 days), was $2.68kg\;NH_3-N\;ha^{-1}$ and $6.58g\;N_2O-N\;ha^{-1}$, respectively, in the control. The injection application of pig slurry decreased total $NH_3$ and $N_2O$ emission by 39.8% and 33.3%, respectively, compared to broadcasting application of pig slurry. The present study clearly showed that injection application exhibited positive roles in reducing N losses through $NH_3$ and $N_2O$ emission.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.3
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• pp.228-233
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• 1993

Method for the sun curing dehydration of pig excreta by using vinyl house was described. We determined effects of the dehydrated pig excreta on the yields of whole crop corn and proximate chemical composition of whole crop corn. Pig excreta were dehydrated upto 15% of moisture content by the 3-4 days of sun curing dehydration in vinyl house in the summer period. There was no significant difference between the dehydrated pig excreta treatment and the chemical fertilizer treatment on dry matter(DM) content, DM yield and crude protein contents of whole crop corn. Ca and Mg contents by the dehydrated pig excreta treatment were lower than those by thechemical fertilizer treatment. In the contrast, K content by the dehydrated pig excreta treatment was higher than those by the chemical fertilizer treatment. and dehydrated pig excreta treatments were higher in K/Ca+Mg ratio than chemical fertilizer treatment. These results suggested the availability of the sun curing dehydration of pig excreta in vinyl house in small-scale animal industry. In addition, the dehydrated pig excreta treatment showen similar effects to the chemical fertilizer treatment on dry matter yields and contents of chemical composition of whole crop corn. These results suggest that using the sun curing dehydration of pig excreta could reduce the chemical fertilizer cost. However, we need more study to the relationship between the unbalanced mineral contents and animal health.

• Biomolecules & Therapeutics
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• v.25 no.4
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• pp.396-403
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• 2017

Under normal, non-stressed conditions, intracellular p53 is continually ubiquitinated by MDM2 and targeted for degradation. However, in response to severe genotoxic stress, p53 protein levels are markedly increased and apoptotic cell death is triggered. Inhibiting the ubiquitination of p53 under conditions where DNA damage has occurred is therefore crucial for preventing the development of cancer, because if cells with severely damaged genomes are not removed from the population, uncontrolled growth can result. However, questions remain about the cellular mechanisms underlying the regulation of p53 stability. In this study, we show that p53-inducible gene 3 (PIG3), which is a transcriptional target of p53, regulates p53 stability. Overexpression of PIG3 stabilized both endogenous and transfected wild-type p53, whereas a knockdown of PIG3 lead to a reduction in both endogenous and UV-induced p53 levels in p53-proficient human cancer cells. Using both in vivo and in vitro ubiquitination assays, we found that PIG3 suppressed both ubiquitination- and MDM2-dependent proteasomal degradation of p53. Notably, we demonstrate that PIG3 interacts directly with MDM2 and promoted MDM2 ubiquitination. Moreover, elimination of endogenous PIG3 in p53-proficient HCT116 cells decreased p53 phosphorylation in response to UV irradiation. These results suggest an important role for PIG3 in regulating intracellular p53 levels through the inhibition of p53 ubiquitination.