• Journal of Sasang Constitutional Medicine
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• v.29 no.2
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• pp.136-153
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• 2017

Objectives This experimental study was designed to investigate the effect of Phaseolus angularis shell on metabolic syndrome. Methods Each 5 C57BL/6J mice were randomly assigned to normal diet group, high-fat diet(HFD) control group, high-fat diet plus 15.6 mg/kg/day of Orlistat(HFD-Orlistat) group, high-fat diet plus 100mg/kg/day of Phaseolus angularis shell extract(HFD-PAS_E) group. Weight, the blood chemical and hematologic parameter was med. The mRNA expression was assayed through Reverse transcriptase polymerase chain reaction(RT-PCR). Results In HFD-PAS_E group, the body weight gain, weight of liver, and the level of LDL-Cholesterol were significantly decreased and the level of HDL-Cholesterol were significantly increased. The size of adipocyte in HFD-PAS_E group was smaller than HFD group's. In HFD-PAS_E group, the expression of leptin, PPAR-${\gamma}$, AP2/FABP4 mRNA in liver adipocyte tissue was decreased, the expression of Adiponectin, UCP-2 mRNA in liver adipocyte tissue was increased and the expression of Leptin, C/EBP-a, AP2/FABP4 mRNA in epididymal adipocyte tissue was decreased. Conclusion These results suggest that Phaseolus angularis shell has inhibitory effects on metabolic syndrome by reducing the body weight and the levels of lipid contents in high-fat-diet induced obese mice.

• Microbiology and Biotechnology Letters
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• v.50 no.2
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• pp.277-282
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• 2022

Whitening effect of the extract of Phaseolus angularis (PA) shell was investigated for its potential application as a functional ingredient for cosmetic products. The tyrosinase inhibitory effect, which is related to whitening, was 76.4% at a concentration of 1,000 ㎍/ml. Cell viability of melanoma cells was measured to test toxicity of the PA shell extract at concentrations showing at least 90% viability. In addition, western blot and RT-PCR assays of the PA shell extract showed concentration-dependent decreases of MITF, TRP-1, TRP-2 and tyrosinase protein and inhibitory effect of mRNA expression. These findings suggested that extract from PA shell has great potential as a cosmeceutical ingredient with whitening effect.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.6
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• pp.802-808
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• 2013

Here we tried to uncover the potential anti-lipogenic effect and the underlying mechanism of Phaseolus angularis seed in a cellular model of nonalcoholic fatty liver disease (NAFLD) induced in HepG2 cells. Ethanol extract of Phaseolus angularis seed (JSD) was prepared. HepG2 cells were incubated in palmitate containing media to induce intracellular lipid accumulation, and co-treated with JSD for 16 hrs before examine intracellular lipid content. In control group, the cells were not co-treated with JSD. We measured the effects of JSD on liver X receptor ${\alpha}$ ($LXR{\alpha}$) and sterol regulatory element-binding transcription factor-1c (SREBP-1c) expression, transcription level of lipogenic genes, including acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-CoA desaturase-1 (SCD-1), and AMP-activated protein kinase (AMPK) activation in HepG2 cells. JSD markedly reduced palmitate-induced intracellular lipid accumulation in HepG2 cells. JSD suppressed $LXR{\alpha}$/SREBP-1c expression, and SREBP-1c mediated induction of ACC, FAS, and SCD-1. Furthermore, JSD activated AMPK, which plays a major role in the control of hepatic lipid metabolism. Taken together, it is suggested that JSD has a potential to alleviate hepatic steatosis, at least in part, by suppressing $LXR{\alpha}$/SREBP-1c mediated induction of lipogenic genes. In addtion, the anti-lipogenic potential may be associated with activation of AMPK. Therefore, the Phaseolus angularis seed could be applied as a potential therapeutics for NAFLD with additional clinical studies.

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.167.2-167
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• 1994

• Journal of Life Science
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• v.21 no.7
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• pp.1060-1066
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• 2011

In order to investigate the variations on the ratio of mannose to galactose in the seeds of Phaseolus angularis, 17 local strains (Yangyang, Pyeongchang, Ganghwa, Pocheon, Geumsan, Seocheon, Jincheon, Danyang, Tongyeong, Sancheong, Gumneung, Wolseong, Wando, Gokseong, Okgu, Jangsu, Bukjeju), which are located from $33^{\circ}15'N$ to $38^{\circ}11'N$, were selected according to their latitudes and geographical distances. The seeds of these strains were collected and their contents of mannose and galactose were analyzed. Mannose contents in the seeds were variable, ranging from 17.071 mg/g at its highest (Jangsu) and 6.488 mg/g at its lowest (Geumsan). The contents of galactose also showed remarkable differences, ranging from 9.477 mg/g (Wolseong) to 19.877 mg/g (Jangsu). The local strains were classified into 3 variation types - coastal type I (Wando, Okgu, Bukjeju), the inland type (Jangsu, Weolseong, Danyang, Geumneung, Pyeongchang, Sancheong) and coastal type II (Ganghwa, Seocheon, Tongyeong, Jincheon), as well as 4 strange strains (Gokseong, Yangyang, Pocheon, Geumsan) according to the geographical climatic type and the ratio of mannose to galactose, which indicate the hardness of seeds in Leguminosae and ranged from 0.64 to 1.22. The variation types are very significant genecologically as evidence for microevolution related to natural and artificial selection in cultivated plants.

• Proceedings of the Korean Society of Crop Science Conference
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• 1988.02a
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• pp.72-73
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• 1988

• Korean Journal of Agricultural Science
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• v.15 no.1
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• pp.69-75
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• 1988

This experiment was carried out for the analysis of the ability of callus formation and differentiation in the eight Korean local varieties of adzuki beans (Phaseolus angularis). KLA 102 has the highest ability on the formation of callus (all tissues have 90 % over). Morphological heterogeneity (color and shape) of callus was well reflected to the cell constitution and cell meristematic activities. Meristemoid tissues and rooting were derived from only creamish-white color and soft region of callus.

• Food Science and Biotechnology
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• v.27 no.6
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• pp.1811-1821
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• 2018

Obesity is generally caused by quantitative changes in adipocyte differentiation and fat metabolism. Only a few studies have been determined the effect of red beans extract on obesity and plasma cholesterol concentration. We have been studied the functional activities of red-bean extracts including anti-oxidative effect against DNA and cell damages. Histological study including micro CT analysis showed that the accumulation of fat in hepatocytes and intestines was significantly decreased in red bean extract treated group. In addition, plasma cholesterol and triglyceride levels were decreased in blood samples. In addition, it was confirmed that the red bean extract inhibited the expression of $PPAR{\gamma}$, Fabp4 and RETN genes, which regulate total adipocyte differentiation and lipid metabolism. Red bean extract inhibits the expressions of transcription factors associated with adipocyte differentiation in a dose-dependent manner, thereby inhibiting fat accumulation and decreasing blood lipid levels in obese mice induced by high fat diet.

• Korean Journal of Plant Taxonomy
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• v.36 no.3
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• pp.217-225
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• 2006

Among the seven taxa of the genus Vigna, Leguminosae vegetated in Korea peninsula, only two taxa, V. unguiculata subsp. unguiculata and V. vexillata var. tsusimensis, are recognized in Vigna. The rests are still remained in the genus Phaseolus in many floras and illustrations of Korea although they were placed into the genus Vigna by Verdcourt in 1970. There has been misidentification between V. angularis var. nipponensis and V. nakashimae because of their similar morphological characters and habit. It was also found that V. nakashimae has not been recorded in many floras. Objectives of this study are 1) to discuss the taxonomic differences between Vigna and Phaseolus, 2) to have a recommendation for using correct scientific names of Vigna taxa, 3) to discuss the taxonomic characteristics of the subgenus Ceratotropis, and 4) to provide a simple taxonomic key of V. angularis var. nipponensis and V. nakashimae.

• Korean Journal of Pharmacognosy
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• v.45 no.3
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• pp.227-232
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• 2014

The seed of Vigna angularis (Ohwi) Ohwi & Ohashi (= Phaseolus angularis W. F. Wight, Leguminosae) is one of well-known folk foodstuffs in Korea, China and Japan. In the course of screening for antioxidants from natural plants in Korea by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) a methanol extract of the seeds of V. angularis were found to show a potent antioxidant activity. And the ethyl acetate soluble fraction of V. angularis (VAEA) showed the most potent DPPH radical scavenging and superoxide quenching activities. To reveal the effect of antioxidant activities of VAEA, we tested the thermal and oxidative stress tolerances, activities of SOD and catalase, and intracellular ROS level in Caenorhabditis elegans. Consequently, VAEA-fed worms lived longer than control worms under the thermal and oxidative stress conditions. And VAEA elevated SOD and catalase activities of worms, and reduced intracellular ROS accumulation in a dose-dependent manner.