• 한국약용작물학회지
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• 제21권2호
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• pp.97-104
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• 2013

The objective of this study is to investigate the optimal condition for macerating enzymatic extraction process that leads to the highest yield and the largest extracted amount of bio-active contents from Rubus coreanus Miq. fruit. The optimal extraction conditions were found as the following: The initial amount of the water added to the fruit was 20 ~ 30% by weight. The mixing ratio used for the macerating enzyme was 4 : 1 : 2 (w : w : w) for cellulase:pectinase:amylogucosidase, and the amount of the macerating enzyme added was 2% by weight. The extraction process was done at a temperature of $45{\sim}50^{\circ}C$ for 10 hours. The extraction yields on Rubus coreanus Miq. fruit by macerating enzymatic extraction process was increased by 84.3% compared to that of hot-water extraction process. The amounts of organic acids and vitamin found in the extract were also higher. The amount of polyphenol and anthocyanin contents in the extract were 185% and 257% of those from hot-water extraction, respectively. These results suggest that macerating enzymatic extraction is an effective method to boost extraction yield and to increase the amount of extraction of bio-active contents from Rubus coreanus Miq. fruit.

• Nutritional Sciences
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• 제9권2호
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• pp.68-73
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• 2006

This study was aimed at investigating hepatic toxicity and immunomodulating effects of defatted methanol extracts of two kinds of medicinal plants, Rubus coreanus Miq. and Atractylodes japonica Koidz. in mice. Defatted methanol extracts of fruits of Rubus coreanus Miq. and rhizome of Atractylodes japonica Koidz. were added at the level of 0.5% or 5%(w/w) to cholesterol-supplemented AIN-76 diet. Each diet was fed to 8 ICR male mice for 30 days. Weight gain and food efficiency ratio of the mice fed 5.0% extract of Rubus coreanus Miq. were significantly lower than those of the mice fed 0.5% extract Relative liver weight and activity of plasma alanine aminotransfernse were significantly increased only in the mice fed 5% extract of Atractylodes japonica Koidz. compared with the others. Splenocyte proliferation was not significantly different between the groups fed 0.5% or 5.0% extract of Rubus coreanus Miq. However, splenocyte proliferation was significantly decreased in the mice fed 5.0% extract of Atractylodes japonica Koidz. compared with that in the mice fed 0.5% Production of interleukin-2 by splenocytes from the mice fed 0.5% extract of Atractylodes japonica Miq. was significantly higher than the control value and it became lower with 5.0% dietary level. Secretion of $interferon-\gamma$ was not significantly different among groups. In conclusion, the defatted methanol extract of Atractylodes japonica Koidz. was likely to exert immunomodulating effect at the level of 0.5% but it may exert adverse effects on immune and liver functions at the level of 5.0%.

• 한국식품영양과학회지
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• 제41권3호
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• pp.327-332
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• 2012

본 연구는 광양 재래종 복분자의 항산화 활성을 평가하였다. 주요 유리당으로는 글루코오스와 과당이 각각 $2.83{\pm}0.01%$, $3.49{\pm}0.17%$로 분석되었다. 복분자의 항산화 활성을 나타내는데 기여하는 비타민 C는 0.04 g/100 g, 비타민 E는 0.01 g/100 g으로 분석되었다. 항산화 기능을 대표하는 광양 재래종 복분자내 안토시아닌 함량은 $2.41{\pm}0.01$ g/100 g으로 분석되었다. 열수추출물과 80% 에탄올추출물의 폴리페놀 함량은 열수추출물 $5.99{\pm}0.15%$, 80% 에탄올추출물 $6.20{\pm}0.41%$로 통계적 유의 차이를 나타내지 않았다. 라디칼 소거 활성을 평가한 결과 동일한 농도에서 DPPH 라디칼 소거활성은 80% 에탄올추출물이 $89.29{\pm}0.68%$, 열수추출물이 $65.04{\pm}3.95%$로 나타났고 ABTS 라디칼 소거활성은 각각 $30.22{\pm}2.91%$, $47.65{\pm}0.45%$로 나타났다. 지질과산화 억제 활성은 80% 에탄올추출물이 $23.27{\pm}0.17%$로 열수추출물 $3.58{\pm}0.21%$보다 높게 나타났다. 안토시아닌을 다량 함유한 광양 재래종 복분자는 이를 함유하는 식품의 가공 및 천연 색소로의 이용이 가능할 것이다. 본 연구의 재래종 복분자의 이화학적 특성은 재래종 복분자 식품의 개발에 활용될 수 있고, 항산화 활성이 높은 80% 에탄올추출물은 건강기능식품의 소재로 활용할 수 있을 것으로 판단되었다.

• 한국자원식물학회지
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• 제19권3호
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• pp.381-384
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• 2006

This study was conducted to selected Korean black raspberry (Rubus coreanus Miq.) for high productivity. The eight major agronomic traits were investigated from 198 clones of the clone bank established in Korea Forest Research Institute, Suwon, Korea. The selection levels based on number of fruit per fructify lateral (NFFL) over 20, and fruit weight (FW) over 1.3g, and yield of individual per fructify lateral (YIFL) over 25g, were applied on 198 clones, resulted in 17 clones selected. The selected superior trees, 17 clones, appeared regional differences for amount of fruiting among 4 different test sites. When number of fruit per fruit petiole (NRFP), fruit weight (FW), yield of individual (YI) and sugar content were satisfied over 20, 1.4g, 6kg and 9.5 brix, respectively, as a select condition, 5 clones were reselected as the superior trees among 17 clones. for 3 years.

• 대한예방한의학회지
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• 제8권1호
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• pp.75-87
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• 2004

Objectives: Rubi Fructus(fruit of Rubus coreanus Miq.) composed of Polygonum multiflorum THUNB. and some medical herbs is known as formula of senescence delay effect. The purpose of this study is to investigate the effect of Rubi Fructus(fruit of Rubus coreanus Miq.) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione peroxidase(GSH-px) in rat erythrocytes and blood plasma. Methods: Sprague-Dawley rats were divided into 3 groups, Normal group(supplied enough water and feeds only, Normal Group), D-galatose administered group(injected D-galatose 50mg/kg, 1time/day for 6 weeks, Control Group) and Rubi Fructus (fruit of Rubus coreanus Miq.) administered group(D-galactose 50mg/kg and Rubi Fructus(fruit of Rubus coreanus Miq.) extracts 85.0mg/200g 1time/day for 6 weeks, BBJ Group). Rats were sacrificed and TBARS, SOD, CAT, GSH-px, Plasma total lipid, Plasma triglyceride and cholesterol were measured in rat erythrocytes and blood plasma. Results : Plasma TBARS concentrations of all experimental group were not significantly different. Red blood cell(RBC) SOD activities of BBJ group was increased, and RBC catalase activities of all experimental group were not significantly different. RBC GSH-px activities of BBJ group was increased. Plasma total lipid concentration of BBJ group were significantly lower than those of control. Plasma triglyceride, total cholesterol and HDL-cholesterol concentrations of all experimental group were not signi ficantly different. Conclusions: According to the above results, it is considered that Rubi Fructus(fruit of Rubus coreanus Miq.) is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 춘계학술발표회
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• pp.95-95
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• 2018

Uric acid is the end product of purine metabolism in human body, originating from hypoxanthine after enzyme catalysis by Xanthine oxidase (XOD). Hyperuricemia results as a result of either over-generation of uric acid or a reduction in its excretion. In silico modelling methods such as Absorption, Distribution, Metabolism, Excretion and Toxicity (ADMET) prediction, Autodock 4.2.6 program were used to study the potential inhibitory compounds of XOD. Also we investigated the inhibition of XOD activity by using the extracts of Dendropanax morbifera and Rubus coreanus Miq spectrophotometrically. According to ADMET data, several compounds from D. morbifera and R. coreanus plants, were found to be more potent in inhibiting the XOD activity than allopurinol. XOD inhibitory activity is evaluated by quantifying the formation of uric acid by measuring the absorbance at 290 m ($A_{290}$).D. morbifera extract inhibited XOD activity at $250{\mu}g/ml$, however the extracts from R. coreanus has inhibited XOD activity at $25{\mu}g/ml$. The major compound of R. coreanus, ellagic acid significantly increased the inhibition rate from $9{\mu}g/ml$ and showed a 71% suppression rate at $15{\mu}g/ml$. Finally, these results suggested a potential inhibitory activities of the extracts from D. morbifera and R. coreanus Miq, but further research is needed to validate to ensure their safe usage as drug.

• 한국약용작물학회지
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• 제11권2호
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• pp.148-154
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• 2003

홍삼(panax ginseng C.A. Meyer) 추출물 단독의 항산화 활성과 홍삼추출물에 삼백초(Saururus chinensis(Lour.) Baill.)와 복분자(Rubus coreanus Miq.) 추출물을 농도별로 보충 급여 하였을 때 나타나는 상승효과를 알아보기 위하여 Sprague Dawley rat에 이들 추출물을 8주간 섭취시킨 후 희생시켜 간장 cytosol중의 superoxide dismutase, catalase, glutathione peroxidase, GST 등의 항상화 효소계 활성을 측정하였다. 그 결과, 홍삼급여군의 superoxide dismutase, catalase, glutathione peroxidase의 효소활성이 대조군에 비해 농도 의존적으로 높았다. 특히 홍삼 단독보다는 삼백초와 복분자를 보충급여시 상승효과가 높았으며 급여기간이 길수록 그 효과가 높았다.

• 한국약용작물학회지
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• 제15권5호
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• pp.324-328
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• 2007

To search for immunoactive natural products exerting anti-inflammatory activity, we have evaluated the effects of the ethanol extracts of Rubus coreanus Miq. (ERC) on lipopolysaccharide-induced nitric oxide (NO), tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$, and $Interferon-{\gamma}\;(IFN-{\gamma})$ production by RAW 264.7 macrophage cell line. Our data indicate that this extract is a potent inhibitor of NO production and it also significantly decreased $IFN-{\gamma}\;and\;TNF-{\alpha}$ production. Consistent with these results, the protein level of inducible Nitric Oxide Synthase (iNOS) and cyclooxygenase-2 (COX-2) was inhibited by ethanol extracts of ERC in a dose-dependent manner. These results suggest that ERC may exert anti-inflammatory and analgesic effects possibly by suppressing the inducible NO synthase and COX-2 expressions.

• 한국식품저장유통학회지
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• 제16권5호
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• pp.618-622
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• 2009

완숙 복분자딸기(Rubus coreanus Miq.) 과실부의 냉동저장 중 품질 특성의 변화를 조사하였다. 저장 초기에 비하여 과실을 저장 할수록 산도는 감소하였고, 가용성고형분은 증가하는 것으로 나타났다. 색상의 경우 저장 초기에는 L, a값이 35.63, 6.74 value로 전체적으로 밝은 붉은 빛을 가졌지만, 냉동 저장되어지면서 28.56, 4.13 value로 유의적으로 감소되어짐에 따라 점차 어두워지고 붉은 빛은 감소하였다. 비타민 C(30.79 mg/100 g)와 엘라그산($155.12\;{\mu}g/100\;mg$) 함량은 저장 초기에 가장 높았으며, 저장 시간이 지날수록 이들의 함량은 감소되었다.

• 한국식품영양과학회지
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• 제37권7호
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• pp.820-825
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• 2008

완숙복분자의 이화학적 특성, 용매분획별 추출물의 항산화활성 및 돈육의 산화억제효과에 대하여 조사하였다. 복분자의 수분, 조단백, 조지방, 조섬유, 조회분, 탄수화물, 산도, pH 및 무기성분 등의 특성을 분석하였다. 총 폴리페놀 및 총 플라보노이드 함량은 ethyl acetate 추출물, butanol 추출물 그리고 ethanol 추출물 순으로 높은 함량을 나타내었다. 각 추출물의 DPPH free radical 소거활성을 농도별로 측정한 결과, ethanol 추출물 $2,000\;{\mg}/mL$와 butanol 추출물 $1,500\;{\mg}/mL$ 농도에서 89.93%와 89.68%의 높은 항산화능을 보였다. 또한 ABTS 양이온 소거활성을 측정한 결과 hexane 추출물을 제외한 모든 추출물 $1,000{\sim}2,000\;{\mg}/mL$에서 90% 이상의 높은 소거활성을 나타내었다. TBARS 방법으로 돈육의 항산화효과를 측정한 결과 ethyl acetate와 ethanol 추출물 공히 TBARS 값이 0.37 MDAmg/kg으로 ascorbic acid 0.39 MDAmg/kg보다 항산화효과가 높게 나타났다. 따라서 복분자 ethyl acetate와 ethanol 추출물은 ascorbic acid보다 산화억제효과가 뛰어난 것으로 생각된다.