• Asian Pacific Journal of Cancer Prevention
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• v.15 no.13
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• pp.5455-5461
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• 2014

Background: Saponins are a major active component for the traditional Chinese medicine, Rubus parvifolius L., which has shown clear antitumor activities. However, the specific effects and mechanisms of saponins of Rubus parvifolius L. (SRP) remain unclear with regard to human chronic myeloid leukemia cells. The aim of this study was to investigate inhibition of proliferation and apoptosis induction effects of SRP in K562 cells and further elucidate its regulatory mechanisms. Materials and Methods: K562 cells were treated with different concentrations of SRP and MTT assays were performed to determine cell viability. Apoptosis induction by SRP was determined with FACS and DAPI staining analysis. Western blotting was used to detect expression of apoptosis and survival related genes. Specific inhibitors were added to confirm roles of STAT3 and AMPK pathways in SRP induction of apoptosis. Results: Our results indicated that SRP exhibited obvious inhibitory effects on the growth of K562 cells, and significantly induced apoptosis. Cleavage of pro-apoptotic proteins was dramatically increased after SRP exposure. SRP treatment also increased the activities of AMPK and JNK pathways, and inhibited the phosphorylation expression level of STAT3 in K562 cells. Inhibition of the AMPK pathway blocked the activation of JNK by SRP, indicating that SRP regulated the expression of JNK dependent oon the AMPK pathway. Furthermore, inhibition of the latter significantly conferred resistance to SRP pro-apoptotic activity, suggesting involvement of the AMPK pathway in induction of apoptosis. Pretreatment with a STAT3 inhibitor also augmented SRP induced growth inhibition and cell apoptosis, further confirming roles of the STAT3 pathway after SRP treatment. Conclusions: Our results demonstrated that SRP induce cell apoptosis through AMPK activation and STAT3 inhibition in K562 cells. This suggests the possibility of further developing SRP as an alternative treatment option, or perhaps using it as adjuvant chemotherapeutic agent for chronic myeloid leukemia therapy.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.9 no.1
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• pp.85-102
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• 1999

In this paper, we propose an EC-SRP(Elliptic Curve - Secure Remote Password) protocol that uses ECDLP(Elliptic Curve Discrete Logarithm Problem) instead SRP protocols’s DLP. Since EC-SRP uses ECDLP, it inherits the high performance and security those are the properties of elliptic curve. And we reduced the number of elliptic curve scalar multiplication to improve EC-SRP protocol’s performance. Also we have proved BC-SRP protocol is a secure AKC(Authenticated Key Agreement with Key Confirmation) protocol in a random oracle model.

• The Transactions of the Korean Institute of Power Electronics
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• v.8 no.3
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• pp.230-238
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• 2003

In this paper, Inverter drives adopting 2 phase space vector SRP-PWM (Separately Randomized Pulse Position PWM) with fixed switching frequency is proposed. The proposed 2 phase space vector SRP-PWM scheme is based on the 3 phase SRP-PWM. In the proposed SRP-PWM scheme, each of two phase pulses is located randomly in each switching interval. The experimental results show that the voltage and acoustic noise harmonics are spread to a wide band area. Also, the performance of the proposed 2 phase SRP-PWM and the conventional center aligned SVM are compared to each other. In result, the speed response is nearly similar to each other from the viewpoint of the v/f constant control.

• Phonetics and Speech Sciences
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• v.11 no.3
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• pp.77-87
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• 2019

The study aimed to develop Speech Range Profile (SRP) and to examine and validate its clinical application. Forty-five participants without voice disorders aged 18-29 years were compared using SRP and Voice Range Profile (VRP). The authors developed the "Fire!" paragraph as a SRP task compromising 14 sentences including all Korean spoken phonemes and sentence types. To compare SRP and VRP results, the participants read the paragraph (reading) and counted from 21 to 30 (counting) as a part of SRP tasks, and produced a vowel /a/ from low to high frequencies (gliding) and a shortened form of the VRP as a part of VRP tasks. $F0_{max}$, $F0_{min}$, $F0_{range}$, $I_{max}$, $I_{min}$, and $I_{range}$ for each task were measured and compared, showing that $F0_{max}$, $F0_{min}$, $F0_{range}$, $I_{max}$, and $I_{range}$ were not different between reading and gliding. $I_{min}$, had the lowest value in counting. It is concluded that the newly developed SRP task, reading the "Fire" paragraph, can yield a maximum phonation range similar to that found by VRP. Therefore, it is expected that voice evaluation can be effectively performed in a relatively short time by applying SRP with the "Fire" paragraph, a functional utterance task, in place of VRP, which may be difficult to measure long term or in cases of severe voice disorders.

• Phonetics and Speech Sciences
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• v.11 no.3
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• pp.49-55
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• 2019

This study sought to investigate whether mean speaking fundamental frequency (SFF) can be predicted by parameters of voice and speech range profile (VRP and SRP) in Korean normal adults. Moreover, it explored whether gender differences exist in the absolute differences between the SFF and estimated SFF (ESFF) predicted by the VRP and SRP. A total of 85 native Korean speakers with normal voice participated in the study. Each participant was asked to perform the VRP task using the vowel /a/ and the SRP task using the first sentence of a Korean standard passage "Ga-eul". In addition, the SFF was measured with electroglottography during a passage reading task. Predictive factors of the SFF were explored and the absolute difference between the SFF and the ESFF (DSFF) was compared between gender groups. Results indicated that predictive factors were age, gender, minimum pitch and pitch range for the VRP (adjusted $R^2=.931$), and pitch range (in semi-tones) and maximum pitch for the SRP (adjusted $R^2=.963$), respectively. The SFF and ESFF predicted by the VRP and SRP showed a strong positive correlation. The DSFF of the VRP and SRP, as well as their sum did not differ by gender. In conclusion, the SFF during a passage reading task could be successfully predicted by the parameters of the VRP and SRP tasks. In further studies, clinical implications need to be explored in patients who may exhibit deviations in SFF.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 1999.10a
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• pp.113-114
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• 1999

일차 오존 표준 분광기(SRP)를 제작하여 오존 측정의 정확성을 확보하고, 이것을 국가 표준으로 이용하여 현장 분석기를 차례로 교정하는 체계(그림 1 참조)를 구축하기 위하여, 먼저 자외선 흡광 광도법을 근간으로 하여 오존 SRP 후보 기기를 제작하였다.(중략)

• Journal of Microbiology and Biotechnology
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• v.21 no.4
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• pp.430-437
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• 2011

The effects of the agitation and aeration rates on the production of serratiopeptidase (SRP) in a 5-L fermentor (working volume 2-l) were systematically investigated using Serratia marcescens NRRL B-23112. The dissolved oxygen concentration, pH, biomass, SRP yield, and maltose utilization were all continuously measured during the course of the fermentation runs. The efficiencies of the aeration and agitation were evaluated based on the volumetric mass transfer coefficient ($K_La$). The maximum SRP production of 11,580 EU/ml with a specific SRP productivity of 78.8 EU/g/h was obtained with an agitation of 400 rpm and aeration of 0.075 vvm, which was 58% higher than the shake-flask level. The $K_La$ for the fermentation system supporting the maximum production (400 rpm, 0.075 vvm) was 11.3 $h^{-1}$. Under these fermentor optimized conditions, kinetic modeling was performed to understand the detailed course of the fermentation process. The resulting logistic and Luedeking-Piret models provided an effective description of the SRP fermentation, where the correlation coefficients for cell growth, SRP formation, and substrate consumption were 0.99, 0.94, and 0.84, respectively, revealing a good agreement between the model-predicted and experimental results. The kinetic analysis of the batch fermentation process for the production of SRP demonstrated the SRP production to be mixed growth associated.

• Proceedings of the KIPE Conference
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• 2004.07a
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• pp.397-401
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• 2004

In this paper, Inverter drives adopting 3-phase and 2-phase SRP-PWM (Separately Randomized Pulse Position PWM) with fixed switching frequency is proposed. In the proposed SRP-PWM scheme, each of 3 or 2 phase pulses Is located randomly in each switching interval. The experimental results show that the voltage / current harmonics and the switching noise harmonics are spread to a wide band area. Also, the performance of the 3-phase SRP-PWM and the 2-phase SRP-PWM are compared to each other. In result, the speed response is nearly similar to each other from the viewpoint of the v/f constant control.

• Journal of Periodontal and Implant Science
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• v.39 no.sup2
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• pp.239-251
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• 2009

Purpose: Full-mouth disinfection enables to reduce the probability of cross contamination from untreated pockets to treated ones, for completing the entire SRP under local anesthesia with chlorhexidine as a mouth wash in two visits within 24 hours. This study aimed to compare the clinical effects of modified full-mouth disinfection (Fdis) after 6 months with those of conventional SRP (cSRP). Methods: Thirty non-smoking chronic periodontitis subjects were randomly allocated two groups. The Fdis group underwent the entire SRP under local anesthesia in two visits within 24 hours, a week after receiving supragingival scaling. A chlorhexidine (0.1%) solution was used for rinsing and subgingival irrigation for Fdis. The cSRP group received SRP per quadrant under local anesthesia at one-week intervals, one week after they had received scaling. Clinical parameters were recorded at baseline, after 1, 3 and 6 months. Results: There are significant (P<0.05) decreases in the sulcus bleeding index, and plaque index, and the increases in gingival recession were significantly smaller with Fdis after six months compared with cSRP. There was significant improvement in the probing depth and clinical attachment level for initially medium-deep pockets (4-6mm) after Fdis compared with cSRP. Multi-rooted teeth showed significantly larger attachment gain up to six months after Fdis. Single-rooted teeth showed significantly more attachment gain, 1 and 6 months after Fdis. Conclusions: Fdis has more beneficial effects on reducing gingival inflammation, plaque level, probing depth, gingival recession and improving clinical attachment level over cSRP.

• BMB Reports
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• v.43 no.4
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• pp.279-283
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• 2010

Polydnavirus genome is segmented and dispersed on host wasp chromosome. After replication, the segments form double- stranded circular DNAs and embedded in viral coat proteins. These viral particles are delivered into a parasitized host along with parasitoid eggs. A serine-rich protein (SRP) is predicted in a polydnavirus, Cotesia plutellae bracovirus (CpBV), genome in its segment no. 33 (CpBV-S33), creating CpBV-SRP1. This study explored its expression and physiological function in the diamondback moth, Plutella xylostella, larvae parasitized by C. plutellae. CpBV-SRP1 encodes 122 amino acids with 26 serines and several predicted phosphorylation sites. It is persistently expressed in all tested tissues of parasitized P. xylostella including hemocyte, fat body, and gut. Its physiological function was analyzed by injecting CpBV-S33 and inducing its expression in nonparasitized P. xylostella by a technique called SERI (segment expression and RNA interference). The expression of CpBV-SRP1 significantly impaired the spreading behavior and total cell count of hemocytes of treated larvae. Subsequent RNA interference of CpBV-SRP1 rescued the immunosuppressive response. This study reports the persistent expression of CpBV-SRP1 in a parasitized host and its parasitic role in suppressing the host immune response by altering hemocyte behavior and survival.