• Annual Conference on Human and Language Technology
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• 2018.10a
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• pp.567-569
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• 2018

한국어의 경어체는 종결어미에 따라 구분하고, 서로 다른 경어체는 각각 고유한 경어 강도가 있다. 경어체 간의 어체 변환은 규칙기반으로 진행되어 왔다. 본 논문은 어체 변환을 위한 규칙 정의의 번거로움을 줄이고 어체 변환 데이터만을 사용한 심층 학습 기반의 어체 변환 방법을 제안한다. 본 연구는 '해요체-합쇼체' 쌍의 병렬 데이터를 이용하여 Attention-based Sequence-to-Sequence 모델을 바탕으로 한 어체 변환 모델을 학습하였다. 해당 모델을 학습하고 실험하였을 때, 정확도 91%의 우수한 성과를 얻을 수 있었다.

• Korean Journal of Microbiology
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• v.30 no.2
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• pp.108-112
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• 1992

To investigate the expression and the secretion of heterologous proteins in yeast, we constructed an yeast secretion vector and produced a human secretory protein, .alpha.-1-antitrypsin (.alpha.-1-AT), from yeast cells. The secretion vector pGAT8 was constructed by inserting the signal sequence of yeast acid phosphatase gene (PH05) into the .alpha.1-AT expression vector pGAT6 which contained .alpha.-1-AT cDNA fused to GAL10-CYC1 promotor. The .alpha.-1-AT was produced efficiently in the yeast cells transformed with plasmid pGAT8, which was onfirmed both by the .alpha.-1-AT activity assay and by the immunoblot method using .alpha.-1-AT antibody. We also showed the secretion of .alpha.-1-AT into the culture media and into the periplasmic space by immunoblot.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.4
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• pp.342-348
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• 2020

Uropathogenic Escherichia coli (UPEC) is a major cause of urinary tract infections (UTIs), which is one of the most common infectious diseases in humans worldwide. Since UPEC is increasingly gaining resistance to many antimicrobial agents, antibiotic therapy of UTI has recently become a great concern. This study examined the epidemiological relationship, and antimicrobial resistance patterns of 84 UPEC isolates obtained from UTI patients in Daejeon, from March to December 2017. Molecular epidemiology was investigated by multilocus sequence typing (MLST), and an antimicrobial susceptibility test was determined using an E-test. In this study, UTI was more common in females (73.8%) than in males (26.2%), and the highest incidence of UTI was observed in the age group in their 70s. Among the 84 UPEC isolates, 59 isolates (70.2%) were multidrug-resistant (MDR), and the major sequence type was ST131 (44 isolates, 52.4%). Interestingly, the rates of MDR in non-ST131 isolates (72.5%) were higher compared to ST131 isolates (68.2%). These results indicate the possibility of the development and spread of MDR in non-ST131 isolates. Effective surveillance networks and continuous research need to be conducted globally to prevent the emergence and international spread of MDR non-ST131 isolates.