• Analytical Science and Technology
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• v.33 no.5
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• pp.224-231
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• 2020

With the improvement in the standard of living and extension of life expectancy, the incidence of prostate diseases has increased yearly, thus becoming a serious disease affecting the health of men. The extract mixture of Cornus officinalis and Stauntonia hexaphylla leaves is a developed functional food formula to improve prostate health. This study developed a simultaneous analytical method of bioactive compounds for quantifying the mixture of Cornus officinalis and S. hexaphylla leaves using high-pressure liquid chromatography-ultraviolet (HPLC-UV). HPLC analytical condition was performed on a Hector C₁₈ column with a mobile phase of 0.1 % formic acid in water (A) and 0.1 % formic acid in acetonitrile (B) under the following gradient conditions: 0-50 min, 12 %-40 % (B) at a flow rate of 1.0 mL/min. Meanwhile, this method was validated properly and successfully used to quantify the bioactive components of morroniside and hederacoside D in 20 sample batches and assess the quality of different ages and seasons of S. hexaphylla leaves. The result showed that the content of morroniside in the extract mixture of Cornus officinalis and S. hexaphylla leaves ranged from 1.38-1.62 mg/g, and the hederacoside D ranged from 28.42-32.02 mg/g, suggesting that this novel analytical method will be suitable for the quality control of the extract mixture to improve benign prostatic hyperplasia.

• Natural Product Sciences
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• v.20 no.2
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• pp.130-134
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• 2014

An investigation was carried out to identify novel anti-cancer compounds from Korean indigenous plant extracts. Bioassay-guided fractionation and chemical investigation of the EtOAc extract from the leaves and stems of Stauntonia hexaphylla resulted in the isolation of two active compounds, hederagenin 3-O-${\alpha}$-L-arabinoside (1) and quercetin (2). The structures of these compounds were elucidated by spectroscopic methods, including UV, IR, MS, NMR techniques and compared with previous spectroscopic data. The cytotoxic effects of fractions and compounds on HCT116 human colon cancer cells were evaluated using the MTT assay. Quercetin showed a stronger anti-cancer effect when compared to hederagenin 3-O-${\alpha}$-L-arabinoside.

• Journal of Physiology & Pathology in Korean Medicine
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• v.34 no.2
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• pp.88-96
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• 2020

Stauntonia hexaphylla (SH) and Vaccinium bracteatum (VB) are herbal extracts widely used in food and traditional herbal medicine, and have the ability to perform a wide range of biological activities. We aimed to investigate the effects of the SH and VB combination (SHVB) on mice models of chronic restraint stress (CRS) and pentobarbital-induced sleeping behaviors to elucidate its possible mechanisms of action. CRS-exposed mice treated with SHVB showed significantly decreased immobility time, increased swimming and climbing times in the forced swim test (FST), and increased locomotor activity in the open field test (OFT). SHVB decreased serum CORT levels, but enhanced brain monoamine neurotransmitters. SHVB significantly decreased the sleep latency and increased total sleep duration in pentobarbital-induced sleeping behavior in mice. SHVB showed inhibitory effect on 5-HT_2A receptor-mediated ERK1/2 phosphorylation. These results suggest that SHVB has antidepressant and hypnotic effects by regulating the 5-HT_2A receptor.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.617-625
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• 2013

Stauntonia hexaphylla and Raphiolepis indica, cold-tolerant broadleaved evergreens ranging through the southern region of South Korea, were assessed on salt tolerance with NaCl treatment using visual damage, chlorophyll florescence image, and malondialdehyde (MDA) analysis. As NaCl concentrations increased, the soil pH decreased and EC increased, and the soil of S. hexaphylla was affected more strongly by the treatment than that of R. indica. In visual damage, S. hexaphylla withered above 200 mM NaCl at 20 days after the treatment. All individuals of R. indica survived during the experiment though the leaves of R. indica showed visual damages up to 400 mM NaCl. The color changes in chlorophyll fluorescence showed a strong correlation with the degree of visual damage. As NaCl increased, the red color of the leaves of S. hexaphylla was distinctly changed to blue and chlorophyll fluorescence decreased starting from the margin to the middle of a leaf. R. indica showed subtle color changes and remained in red color during the experiment. At five days after the NaCl treatment, the MDA of S. hexaphylla was above $4.56nmol{\cdot}g^{-1}$ when plants showed the highest visual damage and EC. The MDA of R. indica in all treatments showed below $1.5nmol{\cdot}g^{-1}$ except 400 mM NaCl treatment during the experiment.

• The Korean Journal of Ecology
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• v.6 no.3
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• pp.208-226
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• 1983

The vegetation of Odongdo island was surveyed over twenty-nine times from April, 1982 to August, 1983. This island is located at 127。46'05'∼127。39'37'E. longitude and 34。40'20'∼34。48'14'N. latitude. The vegetation of this island consisted of 94 families, 261 genera, 314 species, 53 varieties and 6 formae. And among them were 170 species of woody plants and 203 species of herbs. The community of evergreen broad-leaved trees comprised a total of 44 species including Sasa coreana Nakai and camellia japonica L. and the distribution of evergreen broad-leaved trees was good in this island as a whole. Sasa coreana Nakai, Castanopsis cuspidata var. sieboldii Nakai, Ficus erecta Thunb., Ficus nipponica Fr. et Sav, Stauntonia hexaphylla (Thunb.) Decne, Cinnamomum japonicum Sieb., Machilus thunbergii S. et Z., Lozoste lancifolia (S. et Z.) Bl., Ilex intergra Thunb., Camellia japonica Thunb., Hedera rhombea Bean and Ardisia japonica Bl. etc. in this island were autochthonous flora and their preservation is required.

• Journal of Life Science
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• v.28 no.6
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• pp.708-717
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• 2018

The antioxidant activity and protective effects of a hot water extract from the Stauntonia hexaphylla fruit (WESHF) were investigated in vitro and in vivo. The total polyphenol and flavonoid contents of WESHF were $16.13{\pm}0.27mg$ gallic acid equivalent/g and $4.7{\pm}0.80mg$ catechin equivalent/g, respectively. In addition, the DPPH radical-scavenging activity ($SC_{50}$) and the Oxygen Radical Absorbance capacity of WESHF were $63.62{\pm}4.10{\mu}g/ml$ and $90.63{\pm}5.29{\mu}M$ trolox equivalent/g, respectively. The hepatoprotective effect of WESHF against hydrogen peroxide-induced oxidative damage was investigated. $H_2O_2$-induced liver damage on HepG2 cells was prevented by $200{\mu}g/ml$ of WESHF. Furthermore, to investigate the protection mechanism of WESHF on hydrogen peroxide-induced cytotoxicity in HepG2 cells, pre-treatment with $200{\mu}g/ml$ of WESHF significantly attenuated a decrease in the activities of CAT, SOD, GR, and GPx. The hepatoprotective activity of WESHF was evaluated in an experimental model of hepatic damage induced by acetaminophen (APAP). The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly decreased in the livers of mice treated with 200 mg/kg of WESHF compared to the APAP-treated group. The lipid peroxidation level, which increased after APAP administration, was significantly reduced in the WESHF group. In addition, histological examinations of the liver showed the same protective effect of WESHF treatment. Based on these findings, it is suggested that WESHF has potent hepatoprotective effects, and the mechanism that causes this type of protection could be related to antioxidant pathways.

• Korean Journal of Plant Resources
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• v.22 no.5
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• pp.403-411
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• 2009

This study was conducted to gather the basic data on the increase of utilization for the Japanese staunton vine (Stauntonia hexaphylla), native plants which were grown in the southern districts in Korea. We have also determined their partial physical and chemical compositions and their physiological activities. Vitamin C contents in fruit skin was 85.23 mg/100 g, and that in flesh was 61.67 mg/100 g. Total amino acids contents in fruit skin increased much more by 762.72 mg/100 g DW compared to that in flesh by 434.05 mg/100 g DW. Inorganic matter contents were more increased in the fruit skin (108.48 mg/$\ell$) and its main components were K (76.53 mg/$\ell$), Ca (20.20 mg/$\ell$) and Mg (6.22 mg/$\ell$). Total phenol compound and flavonoid contents in 1,000 mg/$\ell$ methanol extracts were 7.3-9.6 mg/$\ell$ and 5.1-6.7 mg/$\ell$. Nitrite radical scavenging activity in 4,000 mg/$\ell$ methanol extracts of fruit skin and flesh for Stauntonia hexaphylla were 79.5% and 77.8%, however, that in seeds was 17.1%. Overall mushroom tyrosinase inhibition activity (% of control) was less than 10.8%. Anti-microbial activities of methanol extracts from the fruit skin against the gram negative and positive microbial strains were not significant in the lower concentration of extracting solution, however, that from flesh and seeds in terms of the inhibition diameter were $8.91{\sim}12.25\;mm$.

• Journal of Environmental Science International
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• v.20 no.1
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• pp.1-23
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• 2011

This study was carried out to investigate the flora of uninhabited Island in the Deokjeok Archipelago. The vascular plants which were collected in 5 times (from Jun. 2009 to Apr. 2010) were identified as 350 taxa in total, including 89 families, 231 genera, 309 species, 5 subspecies, 31 varieties, and 5 forms. There were many useful plants such as 2 taxa of Korean endemics and 2 taxa of rare plants which were proposed by the Korea Forest Service. The specially determined plants which were categorized as 5 degrees in all by the Ministry of Environment appeared as 49 taxa; Degree V was one taxon; Senecio nemorensis and IV was one taxon; Apocynum lancifolium and III was 2 taxa; Stauntonia hexaphylla, Hylotelephium spectabile and I was 45 taxa; Machilus thunbergii, Neolitsea sericea, Hedera rhombea, etc. Based on the plants which were approval objects when transfer overseas were 5 taxa, and 21 taxa of evergreen plants were recorded in the investigated area. The naturalized plants were identified as 15 taxa, and their naturalization ratio were found to be 4.2% respectively.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.4
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• pp.25-40
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• 2011

The vascular plants in Jeokjabong, Bogil Island were listed 350 taxa composed of 89 families, 233 genera, 309 species, 1 subspecies, 36 varieties and 4 forms. Divided into woody plants were 142 taxa (40.6%) and herbaceous plants were 208 taxa (59.4%). Based on the list of rare and endangered species by Korea Forest Service and Korea National Arboretum, 3 taxa were recorded; Celtis choseniana, Asarum maculatum, Rubus sorbifolius, and endemic plants, 10 taxa were recorded; Asarum maculatum, Cephalotaxus koreana, Carpinus coreana, Celtis choseniana, Lespedeza maritima, Indigofera koreana, Dendropanax morbifera, Ligustrum quihoui var. latifolium, Weigela subsessilis, Carex okamotoi, and plant species subject to permission for taking abroad, 4 taxa were recorded; Celtis choseniana, Asarum maculatum, Vaccinium oldhami, Carex okamotoi. Based on the list of floristic regional indicator plants by Korean Ministry of Environment were total 64 taxa (18.3% of all 350 taxa of vascular plants); Ligustrum quihoui var. latifolium in class IV, 19 taxa (Stauntonia hexaphylla, Cinnamomum japonicum, Zanthoxylum ailanthoides, Daphniphyllum macropodum, Centella asiatica, Verbena officinalis, Mitchella undulata, etc.) in class III, 5 taxa (Potentilla dickinsii, Ostericum melanotilingia, Sanicula rubriflora, Caryopteris incana, Teucrium veronicoides) in class II and 39 taxa (Gleichenia dichotoma, Cyrtomium fortunei, Aphananthe aspera, Rubus hirsutus, Meliosma myriantha, Hedera rhombea, Ainsliaea apiculata, etc.) in class I. Based on the list of naturalized plants, 5 families, 17 genera, 20 taxa (Phytolacca americana, Lepidium virginicum, Robinia pseudo-acacia, Verbesina alternifolia, Lolium perenne, etc.) and naturalization rate was 5.7% of all 350 taxa of vascular plants. Hereafter natural ecosystem into disturbance on naturalized plants and legal protection species and rare and endangered species distribute in the Bogil Island, precision investigation after management counterplan of the monitoring a point of view be required.

• Journal of Ecology and Environment
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• v.46 no.4
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• pp.292-303
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• 2022

Background: Climate change significantly influences the geographical distribution of plant species worldwide. Selecting indicator species allows for better-informed and more effective ecosystem management in response to climate change. The Korean Peninsula is the northernmost distribution zone of warm temperate evergreen broad-leaved (WTEB) species in Northeast Asia. Considering the ecological value of these species, we evaluated the current distribution range and future suitable habitat for 13 WTEB tree species designated as climate-sensitive biological indicator species. Results: Up-to-date and accurate WTEB species distribution maps were constructed using herbarium specimens and citizen science data from the Korea Biodiversity Observation Network. Current northern limits for several species have shifted to higher latitudes compared to previous records. For example, the northern latitude limit for Stauntonia hexaphylla is higher (37° 02' N, Deokjeokdo archipelago) than that reported previously (36° 13' N). The minimum temperature of the coldest month (Bio6) is the major factor influencing species distribution. Under future climate change scenarios, suitable habitats are predicted to expand toward higher latitudes inland and along the western coastal areas. Conclusions: Our results support the suitability of WTEB trees as significant biological indicators of species' responses to warming. The findings also suggest the need for consistent monitoring of species distribution shifts. This study provides an important baseline dataset for future monitoring and management of indicator species' responses to changing climate conditions in South Korea.