• Title/Summary/Keyword: W%2FB

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Isolation of Weissella strains as potent probiotics to improve antioxidant activity of salted squid by fermentation

  • Le, Bao;Yang, Seung Hwan
    • Journal of Applied Biological Chemistry
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    • v.61 no.1
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    • pp.93-100
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    • 2018
  • The aim of this study was to enhance the antioxidant activity of salted squid by inoculation of two Weissella spp. strains (W. cibaria FB-069 and W. viridescens FB-077) isolated from traditional Korea salted squid. The safety and probiotic potential characteristics of these two strains were evaluated. The safety of these strains was analyzed based on hemolytic activity, mucin degradation, biogenic amino production, and resistance to antibiotics. These lactic acid bacteria showed probiotic potential, including resistance to gastrointestinal tract conditions, adhesion to Caco-2 cells, and aggregation. The low-salted squid fermented with Weissella strains had consistently higher antioxidant activity through changing their amino acid profiles. Therefore, W. cibaria FB-069 and W. viridescens FB-077 might be good candidates for fermentation of salted squid to develop functional food with enhanced health benefits.

Identification and phylogenetic analysis of the human endogenous retrovirus HERV-W pol in cDNA library of human fetal brain (인간태아의 뇌로부터 유래된 cDNA liberary에서 내생레트로바이러스 HERV-W pol 유전자의 동정과 계통)

  • Kim, Heui-Soo;Jeon, Seung-Heui;Yi, Joo-Mi;Kim, Tae-Hyung;Lee, Won-Ho
    • Journal of Life Science
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    • v.13 no.3
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    • pp.291-297
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    • 2003
  • A human endogenous retroviral family (HERV-W) has recently been described that is related to multiple sclerosis-associated retrovirus (MSRV) sequences that have been identified in particles recovered from monocyte cultures from patients with multiple sclerosis. Two pol fragments (HWP-FB10 and HWP-FBl2) of HERV-W family were identified and analysed by the PCR approach with cDNA library of human fetal brain. They showed 89 percent nucleotide sequence similarity with that of the HERV-W (accession no. AF009668). Deletion/insertion or point mutation in the coding region of the pol fragments from human fetal brain resulted in amino acid frameshift that induced a mutated protein. Phylogenetic analysis of the HERV-W family from GenBank database indicates that the HWP-FB10 is very closely related to the AC000064 derived from human chromosome 7q21-q22. Further studies on the genetic relationship with neighbouring genes and functional role of these new HERV-W pol sequences are indicated.

Mucolytic Effects of Various Parts of FRUCTUS BENINCASAE Extracts in the Rat Trachea (백동과(白冬瓜)와 청동과(靑冬瓜)의 부위별(部位別) 추출물(抽出物)에 의(依)한 거담효과(祛痰效果)의 비교연구(比較硏究))

  • Kim, Yu-Jin;Shin, Min-Kyo
    • The Journal of Korean Medicine
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    • v.20 no.2
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    • pp.165-176
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    • 1999
  • The in vivo effects of Fructus Benincasae (FB-Baekdongkwa(B) and FB- on the expectoration (decrease in sputum viscoelasticity) by their sorts and using rats (Sp. D. male, $150{\sim}160g$). FB was divided by seed, flesh, and bark, extracted by 95% ethanol for 3 hr. The extracts were given to rats administration and the following results were obtained: 1. When FB Recens-C extract was administered at the concentration of 300 mg/kg b.w., mucus secretion effect in the trachea was desirably stimulated. 2. The secretion of phenol red was increased in the FB-treated tracheas in the order of Semen Benincasae(SB)-B $(153{\pm}8\;%)$, FB-B $(149{\pm}10\;%)$, and FBR-C $(117{\pm}26\;%)$. In general, the effect of FB-B extract on phenol red secretion was stronger than that of FB-C. 3. When tracheobronchial lavage fluid was analyzed, the mucus secretion was relatively high $(111{\pm}14\;%)$ in FB-B compared with other extracts. 4. Microscopic analysis after direct treatment of the FB extracts to the rat tracheal tissue showed that all the FB extracts possessed no effects for the activity of the ciliary movement. 5. Glycoprotein content secreted by the seed extract of FB-B was increased compared with the control group, which represents the highest secretion effect of mucus. From the above results. we could conclude that the seed of SB-B possesses better activity for mucus secretion from trachea than the extracts of any other parts. Therefore, it is expected that the seed of SB-B may be available for the purpose of expectorant activity in the prescription of traditional medicine.

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Effect of Food Waste Compost on the Antler-Type Fruiting Body Yield of Ganoderma lucidum

  • Jo, Eun-Young;Cheon, Jae-Lyoung;Ahn, Johng-Hwa
    • Mycobiology
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    • v.41 no.1
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    • pp.42-46
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    • 2013
  • The effects of the composition of a mixture containing food waste compost (FWC), rice bran (RB), and oak sawdust (SD) on the antler-type fruiting body (FB) yield of Ganoderma lucidum were studied. Experiments were performed using 0 (control), 5, 10, 15, 20, 25, 30, 35, and 40% (w/w) FWC added to a basal growth medium consisting of 20% (w/w) RB and 80% (w/w) SD. The content of 15% FWC gave the highest FB yield ($27.0{\pm}1.3$ g/bottle), which was 44% higher than the yield ($18.6{\pm}2.8$ g/bottle) of the control treatment. However, FWC contents of 20~40% showed reduced yield (2.4~23.0 g/bottle), partly because FWC had a high Na concentration (0.6%). These results demonstrate the potential for use of FWC as a component of a growth medium for production of G. lucidum FBs.

FUMONISIN $B_1$IN DEVELOPING RATS ALTERS BRAIN SPHINGANINE LEVELS AND MYELINATTON.

  • Kwon, Oh-Seung;Schmued, Laurence. C.;William Slikker, JR
    • Proceedings of the Korea Society of Environmental Toocicology Conference
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    • 1996.12a
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    • pp.25-41
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    • 1996
  • KWON, O. S. SCHMUED, L. C. and LSIKKER, W. JR. Neurotoxiciligy. objectives of this study were to test the hypothesis that fumonisin $B_1$ ($FB_1$) alters sphinganine (Sa) levels and myelin synthesis in the central nervous system of developing rats. $FB_1$ (subcutaneous, 0. 4 or 0. 8 mg/kg/day) from postnatal days (PND) 3 to PND 12 resulted in a significantly increased in the brain of rats given 0. 8 mg $FB_1$/kg/day. To confirm the effect of limited nutrition on changes in the Sa levels and myelinogenesis, rats given 0.8mg $FB_1$/kg/day or treated by limited nutrition (temporary removal from dam during postnatal period) were compared to those in saline controls. Sa levels and Sa/So ratios were compared to those in saline in the 0.8 $FB_1$-treated, 3'-phosphohydrolase (CNP) activities were decreased significantly in both nutritionally limited and $FB_1$-exposed rats. These data indicate that sphingolipid metabolism in the central nervous system of develiping rats is vulnerable to $FB_1$ exposure. The hypomyelination associated with $FB_1$-treatment may be mediated by limited nutrition.

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The Mechanical Properties and Hand of Cotton Fabrics with the Variation of Weft Density (면직물의 위사밀도 변화에 따른 역학특성 및 태)

  • Bae Jin-Hwa;Park Jung-Whan;An Seung-Kook
    • Science of Emotion and Sensibility
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    • v.8 no.4
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    • pp.345-354
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    • 2005
  • In this study, the mechanical properties and hand characteristics have been analyze4 according to fabric structural parameters such as the weft density and weave stricture of cotton fabric. KES-FB system was used to measure hand characteristics and mechanical properties of fabric. The weft density made an effect on bending and shear properties but not on tensile , compression, and surface properties. In case of wearing property, B/w, 2HG/G, 2HB/B, 2HB/W, $\sqrt[3]{B/W}$, $\sqrt{2HB/W}$, W/T, WC/W were affected tv the weft density. The crimp was highly correlated with the tightness, hand, wearing an4 mechanical properties, specially tensile linearity, bending, shear, and compression properties. The weft crimp influenced the bending, shear, compression resilience, surface roughness, hand, and wearing properties. The tightness has an effect on tile bending, shear, compression, surface friction, hand, and wearing properties.

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Dynamic Analysis and Control-Loop Design of ZVS-FB PWM DC/DC Converter for High-Power Applications (대용량 ZVS-FB PWM DC/DC 변환기의 제어 루프 설계와 동특성 해석)

  • Yoon, Kil-Moon;Baek, Ju-Won;Cha, Young-Kil;Kim, Heung-Geun
    • Proceedings of the KIEE Conference
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    • 1997.07f
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    • pp.2023-2027
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    • 1997
  • This paper presents the dynamic analysis and control-loop design of a zero-voltage-switched full bridge (ZVS-FB) PWM dc/dc converter. Based on the small-signal analysis results, the control-loop is designed using a simple two-pole one-zero compensation circuit. Design results are verified by both computer simulations and experimental data obtained from a 2kW prototype converter.

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Analysis of a FB-ZVS PWM DC/DC Converter for Electric Vehicles (전기자동차용 FB-ZVS PWM DC/DC 변환기의 해석)

  • Lee, Dong-Keun;Yoon, Duck-Yong;Hong, Soon-Chan
    • Proceedings of the KIEE Conference
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    • 1996.07a
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    • pp.461-463
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    • 1996
  • A FB-ZVS(Full Bridge Zero Voltage Switching) PWM DC/DC converter for electric vehicles is analyzed in this paper. The converter considered is a step-down DC/DC converter with the ratings of 3l2/I3.5V and 1.35kW. The steady state analysis of this converter is divided into six operating modes. Digital simulations using PSPICE are carried out to verify the steady-state analysis.

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Dynamic Analysis and Control Loop Design of ZVS-FB PWM DC/DC Converter (ZVS-FB PWM DC/DC 변환기의 동특성 해석 및 제어기 설계)

  • 이득기;윤길문;차영길;김흥근
    • The Transactions of the Korean Institute of Power Electronics
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    • v.3 no.3
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    • pp.231-239
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    • 1998
  • This paper presents the dynamic analysis and control loop design of a zero voltage switching full bridge (ZVS-FB) PWM DC/DC converter. The small-signal model is derived incorporating the effects of phase shift control and the utilization of transformer leakage inductance and power FET junction capacitance to achieve zero voltage resonant switching. These effects are modeled by introducing additional feedforward and feedback terms for duty cycle modulation. Based on the results of the small-signal analysis, the control loop is designed using a simple two-pole one-zero compensation circuit. To show the validity of the design procedures, the small signal analysis of the closed loop system is carried out and the potential of the zero voltage switching and the superiority of the dynamic characteristics are verified through the experiment with a 2 kW prototype converter.

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A Control of the ZVZCS PS-FB DC/DC Converter using All-Pass Filter (전역통과필터를 이용한 ZVZCS PS-FB DC/DC 컨버터의 제어)

  • Cho, Han-Jin;Lee, Won-Cheol;Lee, Sang-Seok;Lee, Su-Won;Won, Chung-Yuen
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.24 no.1
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    • pp.152-159
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    • 2010
  • High power density and power conversion efficiency have been required in the power converters according to the rapid growth of industry. In this context, the next generation High Speed Train(HST) requires power converter which has high-efficiency, high-performance and high-density. In this paper, the new control technique for battery charger used for the next generation HST is proposed. The phase shift ZVZCS converter is classified according to a resonant circuit which is located in the primary or secondary side. In this paper, The PWM switching technique using all-pass filter is proposed to control ZVZCS converter which has resonant circuit in the secondary side. ATmega_128 micro controller based in all-pass filter in substitute for phase shift IC is presented to have digital control. To verify the proposed topology, the simulation and experiment are performed by using PSIM software and 1[kW] experimental set-up.