• Journal of Environmental Health Sciences
• /
• v.8 no.2
• /
• pp.33-46
• /
• 1982

This experiment was carried out for the purpose of reducing alkaloid in reconstituted tobacco sheet and effluent of reconstituted tobacco sheet manufacturing company by treating oxidizing agents such as ozone, sodium hypochlorite, perchloric acid and hydrogen peroxide to tobacco extract created from the manufacturing process of reconstituted tobacco sheet. The effect of alkaloid reduction in tobacco extract by the volume added, time of treatment and pH of oxidizing agents were as follows: 1. When the solid rate of tobacco extract stood at 10 percent, the content of alkaloid, total sugar, total nitrogen and chlorine was 1,600mg/l, 11,000mg/l, 3,200mg/l and 4,000mg/l, respectively. 2. The effect of alkaloid reduction through ozone treatment was in proportion to time of ozone treatment. Alkaloid showed a 31.2 percent reduction under 8 hours' ozone treatment and 0.23g ozone consumed to remove lmg alkaloid. 3. Alkaloid reduction through sodium hypochlorite treatment was influenced by quantity of chlorine in sodium hypochlorite solution. To remove lmg alkaloid, 36.3mg chlorine was used. Reduction of alkaloid was not affected by time of sodium hypochlorite treatment, while showed the best reaction under pH 5-7. 4. The effect of alkaloid reduction by perchloric acid was under the control of the volume added and time of treatment of perchloric acid. The volume of perchloric acid required to remove alkaloid was on the decrease as time of treatment was getting longer. lmg alkaloid was removed by 0.15g perchloric acid under 8 hours' perchloric acid treatment. 5. Alkaloid reduction reacted slowly to the volume added and time of treatment of hydrogen peroxide. Under 8 hours' hydrogen peroxide treatment, it showed maximum removal, registering 10 percent alkaloid reduction.

• Journal of Microbiology and Biotechnology
• /
• v.3 no.4
• /
• pp.238-243
• /
• 1993

In the suspension cultures of Eschscholtzia californica, phytohormone effects showed that alkaloid production was increased by IAA treatment without kinetin in both volumetric and specific way. Kinetin, however, suppressed alkaloid accumulation. Addition of ethephon inhibited cell growth. However, it enhanced the alkaloid production significantly in both volumetric and specific way. IAA promoted alkaloid production during elicitation. The highest alkaloid accumulation was observed at 5 $\mu$ M of IAA. Ethephon also enhanced alkaloid production during elicitation. The highest alkaloid formation was observed at 460 mg/l of ethephon with elicitation. Elicitation with ethephon, however, altered cell growth and the pattern of benzophenanthridine alkaloids production.

• Journal of Radiation Protection and Research
• /
• v.22 no.3
• /
• pp.195-205
• /
• 1997

This paper is to assess the effect of Adaptagen as a radioprotector in which main component is alkaloid fraction of ginseng. Evaluation was made in vitro and in vivo study with NIGP(S) mouse by the measurement of regeneration of jejunal crypt cell and micronucleus assay to analyze radioprotective effect of ginseng alkaloid fraction in comparison with that of water fraction after whole body irradiation. The results were as follows, 1. The degree of radiation damage of mouse jejunal crypt cell was diminished in both of alkaloid and water fraction groups compared to control group but more in alkaloid fraction group than water fraction group. Regeneration of mouse jejunal crypt cell was higher both in alkaloid and water fraction groups than control group. 3. In vitro study, frequency of micronucleus was diminished in tendency for the treated groups than control group but statistically insignificant. 4. In vitro study, frequency of micronucleus was diminished in both alkaloid and water fraction groups compared to control group but more in alkaloid fraction group than water fraction group.

• KSBB Journal
• /
• v.17 no.6
• /
• pp.525-530
• /
• 2002

The optimum culture conditions for tropane alkaloid production in hairy root cultures of Korea native Scopolia paviflora Nak. were investigated. Hairy root was induced from the rhizome of the mother plant on B5 medium containing 1.0 mg/L IBA. Among the culture media examined, 1/2 B5 medium was the best for tropane alkaloid production, whereas the growth of hairy root increased in SH medium. The best result on the growth of hairy root was obtained in 1.0 mg/L NAA, and tropane alkaloid production was obtained in plant growth regulator-free medium. Of the carbone sources tested, 3% sucrose promoted the growth of hairy root, whereas 5% sucrose increased tropane alkaloid production. Optimum inoculum densities for root growth and tropane alkaloid production were 0.5 g and 1 g, respectively. The addition of XAD resins (1 % w/v) to hairy root cultures led to increases in tropans alkaloid production, and the release of alkaloid into the medium and its adsorption by the resin accounted for about 50 to 80％ of total production. It is concluded that optimized culture conditions and the addition of XAD resins could be used in the development of a bioprocess for tropane alkaloid production in hairy root cultures of S. paviflora Nak.

• Korean Journal of Plant Tissue Culture
• /
• v.24 no.3
• /
• pp.137-142
• /
• 1997

In order to elucidate the optimum medium on the growth and tropane alkaloid production of hairy root, hairy root were induced by inoculating leaf and stem of Datura stramonium var. tatula Torr. with Agrobacterium spp. Both Agrobacterium tumefaciens $\textrm{A}_{4}$ T and A, rhizogenes ATCC 15834 among tested strains were effective on hairy root formation. Among 23 clones selected in SH (Schenk and Hildebrandt, 1972) liquid medium, DTLA9 clone was shown fast growth of hairy root and DTLE6 clone was shown high level production of tropane alkaloids. When both DTLA9 and DTLE6 clones were cultured in the GD (Gresshoff and Doy, 1972) medium, alkaloid production was higher than in 8 tested media. It was elucidated that optimum medium for root proliferation and for tropane alkaloid production is SH, GD medium, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.12
• /
• pp.43-48
• /
• 1972

The present experiments were carried out to find out the proper concentration of CEPA(2-chloroethy-lphosphonic acid) for the purpose of accelerating the leaf maturity and of reducing the nicotine content of tobacco. Varried levels of CEPA were sprayed Yellow Special A right after topping. The effects of each level CEPA treatment on leaf ripening and total alkaloid contents were periodically observed. The results are summarized as follows; 1. The higher the CEPA concentration was, the more the leaf maturity was accelerated. During the period from six to 11 days after treatment, the differences of leaf maturity among levels were prominent. 2. Treatment with CEPA only on the upper surface of the tobacco leaf, accelerated the maturity of that particular part treated, but not apparently the other parts of leaf. 3. The higher the CEPA concentration was, the more the accumulation of the total alkaloid was reduced. The reduction of alkaloid accumulation was evident after acceleration of leaf maturity. 4. Distinctive acceleration of leaf maturity was observed in the fully developed lower leaves, while reduction of alkaloid accumulation in the growing upper leaves. The degree of total alkaloid content reduced in the ripened leaves, however, were similer to all the leaves at different positions. 5. Yields of tobacco leaves were not significantly affected by CEPA treatment. 6. In the present experiments, it may be concluded that CEPA2, 000ppm is the most applicable level for accelerating leaf maturity and decreasing total alkaloid content. In the view point of the practical use, however, the applicable level is assumed to be properly choosed between 500 to 2, 000ppm, depending on the situations. 7. The mechanism of accelerating maturity and reducing alkaloid accumulation of tobacco leaves by CEPA, is further to be explored.

• KSBB Journal
• /
• v.8 no.5
• /
• pp.488-494
• /
• 1993

The accumulation of benzophenanthridine alkaloids, sanguinarine, chelerythrine, chelirubine and macarpine occurred in suspension cultures of Eschscholtzia californica. To increase alkaloid production, feeding experiments with the biosynthetic precursors, tyrosine, tyramine, L-dopa, dopamine with and without elicitation were studied. In feeding experiments with various precursors, the total alkaloid production was slightly increased. The precursor feeding with elicitation, however, increased total alkaloid production several times.

• Journal of Microbiology and Biotechnology
• /
• v.1 no.3
• /
• pp.220-226
• /
• 1991

Large and rapid increases in benzophenanthridine alkaloid production occured in suspension cultures of Eschscholtzia californica cells treated with elicitors. Response to different biotic elicitors showed that elicitors prepared from yeast extract, Collectotrichum lindemuthianum and Verticillium dahliae induced alkaloid formation. Highest alkaloid accumulation was obtained with $60\;\mu\textrm{g}$ of yeast extract elicitor per gram of fresh cell weight. In time course performance after elicitor addition, more than 40 hours were required to obtain saturated alkaloid accumulation. Compounded silicone fluid, an ideal accumulation phase for two-phase culture of E. californica, accumulated a large amount of alkaloids produced in a specific manner. Elicitation in two-phase culture clearly increased net alkaloid production as well as their concentrations in the accumulation phase.

• Korean Journal of Plant Tissue Culture
• /
• v.27 no.4
• /
• pp.269-282
• /
• 2000

Benzylisoquinoline alkaloids are a diverse group of natural products that include many pharmacologically active compounds produced in a limited number of plant families. Despite their complexity, intensive biochemical research has extended our knowledge of the chemistry and enzymology of many important benzylisoquinoline alkaloid pathways, such as those leading to the analgesic drugs morphine and codeine, and the antibiotics sanguinarine and berberine. The use of cultured plant cells as an experimental system has facilitated the identification and characterization of more than 30 benzylisoquinoline alkaloid biosynthetic enzymes, and the molecular cloning of the genes that encode at least 8 of these enzymes. The recent expansion of biochemical and molecular technologies has creat-ed unique opportunities to dissect the mechanisms involved in the regulation of benzylisoquinoline alkaloid biosynthesis in plants. Research has suggested that product accumulation is controlled by the developmental and inducible regulation of several benzylisoquinoline alkaloid biosynthetic genes, and by the subcellular compartmentation of biosynthetic enzymes and the intracellular localization and trafficking of pathway intermediates. In this paper, we review our current understanding of the biochemistry, cell biology, and molecular regulation of benzylisoquinoline alkaloid biosynthesis in plants. We also summarize our own research activities, especially those related to the establishment of protocols for the genetic transformation of benzylisoquinoline alkaloid-producing species, and the development of metabolic engineering strategies in these plants.

• Journal of the Korean Society of Tobacco Science
• /
• v.1 no.2
• /
• pp.120-126
• /
• 1979

The nitrogen contents of some forms in Burley tobacco leaf cultivated in Yaesan, Mokpo and Namwon district were investigated. The rate of each form in total nitrogen contents were as follows ; Protein form nitrogen 30~33 % Nitrate form nitrogen 10~123% Alkaloid form nitrogen 8~16 % Ammonia form nitrogen 6~9% Amide form nitrogen 2~ 3% Other form nitrogen 26~44 % The order of nitrate form nitrogen content on the nitrogen of each tobacco cultivated in three area was Yaesan > Namwon > Mokpo, but that of alkaloid form nitrogen was reverse order of nitrate form nitrogen. As for Quality ( grade ), the orders of alkaloid and ammonia form nitrogen content on total nitrogen were H5> 3> 1> L 1> 3> 5, but that of nitrate form nitrogen was reversed.