• Biomolecules & Therapeutics
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• v.10 no.4
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• pp.211-217
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• 2002

Hepatoprotective activity of methanol extract of Angelica tenuissima Nakai on the $CCl_4$-induced hepatotoxicity was investigated. To elucidate the hepatoprotective activity and free radical scavenging effect, we examined alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin, total protein, cholesterol, malondialdehyde (MDA) levels in serum and activities of superoxide dismutase (SOD), catalase (CAT) in hepatic tissue as compared with those of carbon tetrachloride-induced rats. The action mechanism also has been estimated by quantative analysis of cytochrome P450 (CYP), NADPH-CYP reductase for phase I metabolism and glutathion (GSH), glutathion S-transferase (GST) level for phase II metabolsim. Treatment of Angelica tenuissima methanol extract significantly lowered the levels of alanine aminotransferase and aspartate aminotransferase. In addition, the levels of cholesterol, triglyceride, MDA, CAT were decreased, and SOD was activated. This result indicates that the hepatoprotective effect of Angelica tenuissima methanol extract on the CCl4-induced hepatotoxicity would be originated from reduction of the NADPH-CYP reductase, GSH and the enhancement of the activities of GST, CYP.

• The Korea Journal of Herbology
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• v.20 no.4
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• pp.33-39
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• 2005

Objectives : Because Ligusticum sinense Root as well as those of Angelica tenuissima Root are sole as Gobon(藁本) in domestic markets, morphological characteristics of them are attempted to be distinguished. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Results : 1. Angelica tenuissima Root has considerable number of thick roots, but Chinese Ligusticum sinense Root and Ligusticum jeholense Root are composed of rhizomes mostly with small roots. 2. Intersection of rhizomes of Angelica tenuissima Root shows a 'V'-like arrangement of xylem vessel. 3. Chinese Ligusticum sinense Root and Ligusticum jeholense Root did not show the 'V'-like arrangement, and sliced fiber bundles were found in xylem. 4. There was no difference between Chinese Ligusticum sinense Root and Ligusticum jeholense Root. Conclusions : In conclusion, Angelica tenuissima Root, Chinese Ligusticum sinense Root, and Ligusticum jeholense Root show characteristic differences in internal forms, so that it is possible to distinguish them with optical microscope.

• Proceedings of the Korean Society of Crop Science Conference
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• 1990.05a
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• pp.118-119
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• 1990

• Korean Journal of Plant Resources
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• v.11 no.3
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• pp.279-282
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• 1998

This expriment was carried out to get basic information on composition and yield of aromatic constituents in leaves of four medicinal plants, Angelica tenuissima, Chrysanthemum zawadskii. ssp. latilobum, Artemisia iwayomogi and Artemisia capillaris. Volatile aromatic constituents, 28 compounds in Angelica tenuissima were identified and 19 compounds were indentified in Chrysanthemum zawadskii ssp. latilobum. Volatile aromatic constituents, 23 compounds in Artemisia iwayomogi and Artemisia capillaris were identified. Major volatile aromatic consitiuents analyzed by GC/MS in four plants were $\alpha$-pinene, camphene, sabinene, cis-2-hexanol, and camphor etc. Content of essential oils in Angelica tenuissima, Chrysanthemum zawadskii ssp. latilobum, Artemisia iwayomogi and Artemisia capillaris were 0.014, 0.275, 0.785, and 0.452%, respectively. As a result, it was suggested that a medicinal plant, Artemisia iwayomogi, was worthy of using as a useful material of perfume.

• Korean Journal of Pharmacognosy
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• v.20 no.1
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• pp.13-20
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• 1989

In continuation of our studies on essential oils of Angelica genus(Umbelliferae), We report on the components of essential oils obtained from the root of Angelica tenuissima Nakai(藁子). Oils were obtained from the dried roots by steam distillation and fractionated by column chromatography. Each isolate or fraction was identified by GC, GC-MS and spectral analysis. Essential oils of the root of A. tenuissima(Gaoben) were found to contain $\alpha-pinene,\;camphene,\;\beta-pinene,\;myrcene,\;\alpha-phellandrene,\;\Delta-3-carene,\;p-cymene,\;limonene,\;\gamma-terpinene,\;terpinolene,\;4-vinylguauacol,\;\gamma-elemene$, one aromatic compound, three unidentified sesquiterpene alcohols, butylidenephthalide, senkyunolide and Z-ligustilide which was the most abundant compound comprising 75% of the whole oil. Also butylphthalide and hydroxybutylidenephalide were tentatively identified.

• Natural Product Sciences
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• v.19 no.4
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• pp.297-302
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• 2013

The current study was conducted to evaluate the antibacterial and antioxidant activities of the essential oil fraction from the roots of Angelica tenuissima Nakai and its main components. We extracted the essential oil fraction from the roots of A. tenuissima using steam distillation and isolated its main components. Their antibacterial activities were determined by broth dilution test against food-borne pathogenic bacteria. Antioxidant activities were evaluated by DPPH-scavenging assay and reducing-power test. Also tested was their ability to inhibit the growth of two gastrointestinal cancer cell lines, Caco-2 and MKN-45. The A. tenuissima oil fraction and its main components, ligustilide and butylidene phthalide exhibited marked inhibitory effects against most of the tested antibiotic-susceptible and antibiotic-resistant bacterial strains with minimum inhibiting concentrations (MICs) from $0.21{\pm}0.08$ to $3.60{\pm}0.89mg/ml$. They also showed growth-inhibiting activity against Caco-2 and MKN-45 cells. The oil fraction showed significant antioxidant activities in DPPH radical scavenging assay and reducing-power test. Taken together, A. tenuissima essential oil could be used as a safe additive for preventing food contamination by pathogenic bacteria. Additionally, its antioxidative activity and the ability to inhibit gastrointestinal carcinoma cell lines could increase its value for functional foods and prevention of cancer.

• Korean Journal of Medicinal Crop Science
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• v.16 no.3
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• pp.168-172
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• 2008

The root of Angelica tenuissima Nakai (Umbelliferae) has been used in traditional medicines of Korea as a headache, common cold and a fever remedy. A. tenuissima contains ferulic acid and various compounds of essential oil group such as limonene, 3-butylidenephthalide, ${\gamma}$-terpinene, neocnidilide, ligustilide, senkyunolide and neocnidilide. This study carried out to compare the contents of ferulic acid, z-ligustilide and n-butylidenephthalide between native and cultivated species of A. tenuissima by HPLC. The average contents of ferulic acid, z-ligustilide and n-butylidenephthalide indicated that native species (9 samples) were 0.060%, 0.616%, 0.025% and cultivated species (15 samples) were 0.037%, 0.141%, 0.029%, respectively. All samples were collected from different places in Korea.

• Korean Journal of Pharmacognosy
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• v.2 no.2
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• pp.87-88
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• 1971

Silica gel column chromatography on the ether extract from the fruits of Angelica tenuissima $N_{AKAI}$ gave three kinds of crystalline constituents. The following derivatives of furocoumarins and steroid were identified by UV, IR, NMR spectra, elemental analysis and physico-chemical tests: iso-imperatorin m.p. $108{\sim}109^{\circ}\;C_{16}H_{14}O_4$, prangolarine m.p. $103{\sim}104^{\circ}\;C_{16}H_{14}O_5$, ${\beta}-sitosterol$ m.p. $138{\sim}139^{\circ}\;C_{29}H_{50}O$.

• Proceedings of the Korean Society of Crop Science Conference
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• 1988.02a
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• pp.104-105
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• 1988

• The Korea Journal of Herbology
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• v.22 no.3
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• pp.1-7
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• 2007

Objectives : This research was observed by examining the external and internal parts and the quantity of the surface components of the wild type and cultivar of Angelica tenuissima in korea. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by the microscope and examined the quantity of the surface components. Results : 1. The cultivar has comparatively many thick roots while the wild type has many radicles, with its root and stem constituting the major part, and the size of its root is small. 2. In case of ferulic acid, the wild type contains 0.066% of it, while the cultivar, 0.031%, The wild type contains 4.258% of z-ligustilide and the cultivar, 1.956%. Conclusions : It was thought that there might be also some differences in their genetic characteristics, etc. On this, however, further in-depth study is needed.