• 약학회지
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• 제19권1호
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• pp.16-20
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• 1975

A polyacetylenic compound ws isolated from the subterranean parts of Bupleurum falcatum L. (Umbelliferae) and its structure was elucidated as pentadecadiene-(2, 9)-diyn-(4, 6)-ol(1) acetate.

• 한국자원식물학회지
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• 제11권3호
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• pp.283-289
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• 1998

To determine the differences among cultivated , non-cultivated and imported bupleurum falcatum, contents and compositions of fatty acids and saikosaponins which are known as a major biologically acitive compounds in Bupleurum falcatum were meausred with GC and HPLC. Color of grinded powder of Bupleuri radix determined by colorimeter showed that the imported one has the highest white and yellow coloer Among the Bupleuri radix cultivated in China, Hong-siho(red-Bupleurum) produced in Yungil Province contained the highest red color. Cultivated Korean Bupleurum radix contained higher saikosaponi a, c, and than the imported ones which were cultivated in Yungil, Ankuk , and Gillium Province in China. Levels of saikosaponin c are 3 times higher in cultivated Korean Bupleuria radix compared the with imported ones. Furthermore, Bupleurum falcatum cultivated in Korea contained higher amount of palmitic and linoleic acids than those of improted and non-cultivated ones. Particularly they contain two to six times higher linoleic acid.

• 생약학회지
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• 제27권4호
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• pp.354-358
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• 1996

This experiment was conducted to find out the effects of L-phenylalanine on the saikosaponin content of callus induced from Bupleurum falcatum leaf segments. In the fresh and dry weight of callus, the addition of 2,4-D than L-phenylalanine was significantly effective. However, the L-phenylalanine treatment rather than 2,4-D was effective for high saikosaponin accumulation in the callus of Bupleurum falcatum.

• 한국작물학회지
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• 제41권3호
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• pp.384-394
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• 1996

시호 종자의 발아율을 증가시키기 위한 방법과 종자의 특징을 연구하기 위하여 본 실험을 수행하였다. 시호의 발아에 유리한 온도는 2$0^{\circ}C$이며, 호르몬 처리나 기타 물리화학적 처리에 의해 발아율은 크게 증가되지 못했는데, 15$^{\circ}C$에서 발아시킬 경우 50~200 ppm의 GA$_3$을 처리했을 때 발아율이 2배 이상 증가하였다. 또한 같은 온도에서 $10^{-2}$ ~$10^{-3}$M의 KNO$_3$을 처리한 경우에도 발아율은 3배 이상 증가하였다. 그러나 2$0^{\circ}C$ 이상에서는 강력한 저해 효과를 나타내었다. 시호의 leachate를 상추의 종자에 처리했을 경우 발아율의 변화가 거의 없었으므로 시호의 종자에는 발아저해제가 거의 없는 것으로 생각된다. 해부현미경과 주사전자현미경으로 종자의 배와 주공을 관찰한 결과 배가 있는 것과 없는 것의 비율이 거의 50/50이며 주공 자체에는 문제가 없으므로 시호의 종자 발아율이 낮은 것은 근본적으로 배가 결여된 종자가 많기 때문인 것으로 생각된다.

• 대한한방내과학회지
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• 제25권4호
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• pp.9-17
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• 2004

Objective : Mesangial cell proliferation and excessive accumulation of extracellular matrix (ECM) proteins is the common pathologic feature of glomerulosclerosis, and platelet-derived growth factor (PDGF) BB-chain, transforming growth factor betal $(TGF-{\beta}1)$, cyclin dependent kinases (CDK) and CDK inhibitors mediated in these pathophysiological processes. Bupleurum falcatum which is one of the most widely used components in traditional oriental medicines, has multiple pharmacological effects, such as antipyretic, analgesic, immune modulating, anti-inflammatory, anti-allergic, anti-thrombotic, anti-atherosclerotic, and antitussive effects. Methods : In this study, we evaluated the influence of Bupleurum falcatum on mesangial cell proliferation, DNA synthesis and expression of PDGF-BB chain, $TGF-{\beta}1$, CDKI, CDK2, CDK4, p21 and p27 in fetal bovine serum (FBS)-activated human mesangial cell. Results : Bupleurum falcatum reduced the mesangial cell proliferation and DNA synthesis more than control and captopril. And in the ELISA analysis of $TGF-{\beta}1$, and RT-PCR of PDGF-BB chain, CDK1, CDK2, CDK4, p21, and p27, Bupleurum falcatum inhibited the expression of $TGF-{\beta}1$ protein and PDGF-BB, CDK1, CDK2 gene and promoted that of p21 gene in a dose-dependent manner in comparing with control and captopril. Conclusions: These results suggest that Bupleurum falcatum may inhibit the mesangial cell proliferation and DNA synthesis by regulation of PDGF-BB and $TGF-{\beta}1$ expressions, and by modulation of CDK1, CDK2 and p21 expression.

• Plant Resources
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• 제4권2호
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• pp.102-106
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• 2001

To develop optimumal storage method of root of Bupleurum falcatum Radix grown on major cultural herbal medicine materials, root of Bupleurum falcatum Radix was stored for 10 months with different packing materials and sealing methods. The loss in dry weight as influenced by packing materials and sealing methods was the lowest at vacuum packing and followed by complete sealing methods with transparent polyethylene film. The ratio of root rot during the storage period was not significantly different between packing materials but was significantly different between sealing conditions. Conclusionally, vacuum packing and complete sealing with polyethylene film appears to be optimal for storage of Bupleurum falcatum Radix.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2009년도 추계학술대회
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• pp.1087-1090
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• 2009

본 연구는 약용식물로부터 항균활성 물질을 찾기 위하여 Glycyrrhiza uralensis, Dryopteris crassirhizoma, Dictamnus albus, Paeonia lactiflora, Angelica dahurica, Spirodela polyrhiza, Cimicifuga heracleifolia, Bupleurum falcatum, Magnolia kobus, Artemisia princeps, Arctium lappa, Aster tataricus, Hovenia dulcis, Citrus unshiu, Asparagus cochinchinensis, Gardenia jasminoides, Smilax china, Hovenia dulcis, Prunus sargentii, Scutellaria baicalensis등의 약용식물을 사용하였다. 총 20종류의 약용식물로부터 에탄올 추출물을 분리하여 Agrobacterium tumefaciens에 대한 항균활성을 조사하였다. Glycyrrhiza uralensis, Dryopteris crassirhizoma, Cimicifuga heracleifolia, Bupleurum falcatum,의 추출물이 Agrobacterium tumefaciens에 대한 항균활성을 나타냈다.

• 생약학회지
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• 제31권1호
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• pp.63-71
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• 2000

'Si Ho'(柴胡) is one of the chinese crude drugs used mainly to cure inflammation, fever, cough, hepatitis, jaundice, etc. Though the botanical origin of the crude drug has been considered to be Bupleurum species of Umbelliferae, there has been no pharmacognostical study. To clarify the botanical origin of 'Si Ho', the morphological and anatomical characteristics of the roots of Bupleurum species growing in Korea, such as Bupleurum euphorbioides Nakai, Bupleurum falcatum L., Bupleurum leveillei Bois., Bupleurum longiradiatum Turcz. and Bupleurum scorzoneraefolium Wild. were studied. As a result, it is authentified that 'Si Ho' was proved to be the root of Bupleurum falcatum and B. scorzoneraefolium and 'Jug Si Ho' was the root of B. longiradiatum.

• Journal of Applied Biological Chemistry
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• 제64권1호
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• pp.57-61
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• 2021

Saikosaponin derivatives such as saikosaponins A, B1, B2, B3, B4, C, and D present in Bupleurum falcatum were analyzed by a high performance liquid chromatograph equipped with an evaporative light scattering detector, using different extraction solvents (water and 70% ethanol). The samples were injected into a YMC Pack Pro C18 column and separated using a gradient elution system with a mobile phase composed of acetonitrile and water at a flow rate of 1.1 mL/min. The content of saikosaponin derivatives was higher in 70% ethanol extract than in water extract. This study provides an efficient analytical method for determining the optimal conditions for extraction of saikosaponin derivatives, which can be used as a basis for development of functional foods and pharmaceutical products from B. falcatum.

• Journal of Plant Biotechnology
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• 제3권1호
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• pp.1-5
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• 2001

Traditional culture technology of medicinal plants mainly depends on the field culture, which has many problems. With progress of modern culture technology, it has become possible to produce valuable secondary metabolites from medicinal plants. In this paper, we discuss about the pigment and saikosaponin production from too medicinal plants, Carthamus tinctorius and Bupleurum falcatum, through bioreactor culture system. A two-stage bioreactor culture system was established for the production of yellow and red pigments and saikosaponins by cell suspension cultures of Carthamus tinctorius and Bupleurum falcatum. In Carthamus tinctorius, balloon type airlift bioreactors and column type airlift bioreactors were employed for the tell culture and for the pigment production, respectively. The greatest pigment production was obtained on White medium supplemented with 4 mg/L kinetin, high levels of sucrose concentration and photosynthetic photon flux. In Bupleurum falcatum, adventitious roots were cultured in balloon type airlift bioreactors and the root growth was greatest on SH medium containing 5 mg/L IBA and 0.2 mg/L kinetin. HPLC analysis showed that the contents of main active saikosaponins a, c, and d in adventitious roots were almost the same as those in field cultured root.