• Archives of Pharmacal Research
• /
• 제9권3호
• /
• pp.183-187
• /
• 1986

Some of organophosphate pesticides which are the most heavily used in Korea, were examined for their effects on the murine immune system. Immunotoxicological assay parameters adaopted in this study were Arthus reaction for humoral immunity, delayed type hypersensitivity reaction for cell mediate immunity, carbon clearance for macrophage function and susceptiility to tumor challenge. Subchronic exposure of rodents to the pesticides resulted in the marked suppression of immune functions and enhancement of susceptibility to tumor challenge. Among the pesticides tested (fenitrothion, fenthion, diazinon and EPN), fenitrothion was the most suppressive in Arthus and delayed type hypersensitivity reaction.

• 한국식품과학회지
• /
• 제33권1호
• /
• pp.146-148
• /
• 2001

5가지 식용식물인 신이(Flos magnoliae), 지실(Poncirus trifoliata), 시호(Bupleurum falcatum), 황금(Scutellaria baicalensis), 및 오미자(Schisandra chinensis)의 열수 추출물(10 mg/20 g B.W.)이 제IV형 알레르기 반응에 미치는 억제효과를 관찰하기 위하여 DNFB로 감작된 6주령의 웅성 BALB/C mouse에 1, 2차 시료를 투여한 후, 유도된 접촉성 피부염에 의한 부종도를 측정하였으며 대조 약물로는 cyclosporin(0.005 mg/20 g B.W)을 사용하였다. DNFB의 피부염증반응에 대한 귀 두께 측정은 1차 감작에서 황금, 오미자, 지실 군이 대조 군에 비하여 유의한 감소를 나타냈고(p<0.05), 약물 대조군인 cyclosporin과 같은 억제효과를 보였다. 시호, 신이 군은 대조 군에 비해 감소하는 경향을 보였으나 유의한 차이는 없었다. 억제 효과는 황금>오미자>지실>시호>신이의 순으로서 황금 군의 활성이 가장 높았다. 2차 감작에서는 오미자, 지실, 황금, 신이 군이 대조 군에 비하여 유의한 감소를 나타냈고 오미자와 지실은 약물 대조군인 cyclosporin과는 유의적인 차이는 없었지만 억제 효과가 더 컸다. 시호군은 대조 군과 유의적인 차이가 없었으며 오미자>지실>황금>신이>시호의 순으로서 오미자 군이 가장 높은 억제효과를 나타냈다.

• 한방안이비인후피부과학회지
• /
• 제13권2호
• /
• pp.165-181
• /
• 2000

Sinichengpaeum has been widely used in the oriental medical treatment of nasal diseases. These studies were performed done to investigate the effect of Sinicheng- paeum on the anti-allergic action We studied the vascular permeability response induced by the histamine and serotonin injection(allergy Ⅰ type), homologous PCA provoked by the IgE-like antibody against EWA(allergy Ⅳ type), contact dermatitis induced by picryl chloride(allergy Ⅳ type), delayed type hypersensitivity response to SRBC (allergy Ⅳ type), and the amount of eosinophil and IgE. The results were as follows: 1. The effect of Sinichengpaeum on vascular permeability responses to intradermal histamine and serotonin were significant. 2. In the homologous PCA provoked by the IgE-like antibody against white egg albumin, Sinichengpaeum showed a significant effect. 3. In the delayed type hypersensitivity responses to picryl chloride, Sini-chengpaeum provoked a significant effect. 4. After reaction provoked by picryl chloride, Sinichengpaeum showed an insignficant effect on amount of eosinophil, but a significant effect in IgE amount. 5. In the delayed type hypersensitivity responses to SRBC, Sinichengpaeum provoked a significant effect. 6. After reaction provoked by SRBC, Sinichengpaeum showed a significant effect on amount of cosinophil, but an insignificant effect in IgE amount.

• 대한한방내과학회지
• /
• 제15권2호
• /
• pp.260-273
• /
• 1994

This study attempted to investigate the effects of Sojagangkitang and Gamisojagangkitang on the variation of lung thiobarbituric acid(TBA) value, tracheal glycoprotein, serum sodium ion$(Na^+)$ contents, serum potassium ion$(K^+)$ contents ; immediatly type allergy reaction, delayed type allergy reaction in rats and mice. The results were as follows: 1. Sojagangkitang and Gami-sojagangkitang revealed significant effect on immediatly type hypersensitivity responds to histamine. 2. Sojagangkitang and Gami-sojagangkitang revealed significant effect on delayed type hypersensitivity responds to picryl chloride. 3. Sojagangkitang and Gami-sojagangkitang revealed significant effect on delayed type hypersensitivity responds to SRBC, effect of Gami-sojagangkitang was outstanding. 4. Lung thiobarbituric acid(TBA) value was decreased with statistical significance. 5. Sojagangkitang and Gami-sojagangkitang revealed decreasing effect on Tracheal glycoprotein contents, effect of Gami-sojagangkitang was outstanding. 6. Sojagangkitang and Gami-sojagangkitang revealed decreasing effect on phenol red excretion of respiratory tract. 7. Viscosity of mucine solution was decreased in proportion to increasing dosage of the Sample. 8. Serum $Na^+$ contents was not recognized significance. 9. Sojagangkitang and Gami-sojagangkitang revealed decreasing effect on Serum $K^+$ contents, effect of Gami-sojagangkitang were outstanding. According to the above results, it seems that Sojagangkitang and Gami-sojagangkitang can be applied for asthma, chronic obstructive pulmonary diseases, allergic respiratory diseases.

• Archives of Pharmacal Research
• /
• 제15권1호
• /
• pp.20-29
• /
• 1992

Effects of squalene on cellular and non-specific immune responses and antitumor activity in mice were investigated. Cellular and non-specific immunological assay parameters adopted in the present study were delayed-type hypersensitivity reaction and resette forming cells (RFC) for cellular immunity, activities of natural killer (NK) cells and phagocyte for non-specific immunity. Squalene resulted in marked increases of cellular and non-specific immune functions and enhancement of host resistance to tumor challenge in dose-dependent manner.

• IMMUNE NETWORK
• /
• 제2권2호
• /
• pp.65-71
• /
• 2002

The immunological mechanism of the responses to ultraviolet (UV) B radiation in mouse models were investigated by the suppression of contact hypersensitivity (CHS) and delayed type hypersensitivity (DTH), and susceptibility to infection. However, there are some differences in immune suppression according to the different models as well as the irradiation protocols. Therefore, this review focused on the differences in the suppressive effects on CHS and DTH, and susceptibility to infection in relation to the different in vivo models. Recent advances in cytokine knockout mice experiments have the reexamination of the role of the critical cytokines in UVB-induced immune suppression, which was investigated previously by blocking antibodies. The characteristics of the suppressor cells responsible for UVB-induced tolerance were determined. The subcellular mechanism of UVB-induced immune suppression was also explained by the induction of apoptotic cells through the Fas and Fas-ligand interaction. The phagocytosis of the apoptotic cells is believed to induce the production of the immune suppressive cytokine like interleukin-10 by macrophages. Therefore, the therapeutic UVB response to a skin disease, such as psoriasis, by the depletion of infiltrating T cells could be considered in the extension line of apoptosis and immune suppression.

• KSBB Journal
• /
• 제7권1호
• /
• pp.45-50
• /
• 1992

항체 유도 염증 반응인 항체 매개형 과민 반응과 지연형 과민 반응은 족척 두께가 다당류에 의해 거의 정상 수준까지 회복되었다. anilline으로 유도한 methemoglobin의 함량도 거의 차이가 없었다. PFC/spleen cell과 PEC/$10^5$ slpeen cell의 경우 다소 증가를 나타내었으나, PEC는 뚜렷한 증가나 감소를 나타내지 않았으며, 적혈구 응집소가는 다소 증가하였으나 적혈구 용혈소가는 거의 차이가 없었다. 마우스의 plasma corticosterone의 농도는 사육실 및 다당류 투여에 따른 stress요소는 거의 없었다.

• IMMUNE NETWORK
• /
• 제8권1호
• /
• pp.13-20
• /
• 2008

Background: Coptis chinensis rhizome has been used as a medicinal herb in traditional Oriental medicine. We investigated the effects of Coptis chinensis extract on inflammatory mediators and delayed type hypersensitivity in mice. Methods: The inhibitory effect of ethanolic extract of Coptis chinensis (CCE) on cell proliferation was evaluated using MTS assay. The lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and the Con A-activated mouse splenocytes were cultured with various concentrations of CCE. Total nitric oxide (NO) production was determined by Griess reaction. The amounts of secreted prostaglandine E2 ($PGE_2$), interleukin (IL)-2 and IFN-${\gamma}$ were measured by ELISA. To investigate the in vivo anti-inflammatory effect of CCE, oxazolone-induced delayed type hypersensitivity (DTH) model was used. Results: The CCE at $100{\mu}g/ml$ significantly blocked the LPS-induced production of pro-inflammatory mediators (NO and PGE) in RAW264.7 macrophages. Also, it significantly inhibited cell proliferation and cytokine (IL-2 and IFN-${\gamma}$) production in splenocytes. Furthermore, when splenocytes from CCE fed mice (200 mg/kg for 2 weeks) were activated with Con A, cell proliferation and cytokine production were significantly inhibited. In addition, CCE decreased in vivo inflammation in oxazolone-induced DTH model mice. Conclusion: We suggest that Coptis chinensis can be used as an anti-inflammatory drug by exerting an inhibitory effect in inflammatory mediator- and cell-mediated inflammation.

• 약학회지
• /
• 제34권5호
• /
• pp.348-364
• /
• 1990

The activities of twenty-one flavonoids and their related compounds on the hypersensitivity reaction against various antigens were studied in vitro and in vivo. 1. Generally flavonoids inhibited significantly the homologous passive cutaneous anaphylaxis (PCA) induced by reaginic antibody as compared as anaphylaxis by compound 48/80-induced mast cell degranulation, and so more strongly active in the IgE-mediated anaphylaxis than non-IgE-mediated anaphylaxis. 2. Flavonids inhibited remarkably Arths reaction, hemolysin titer, delayed hypersensitivity, haemagglutinin titer, rosette forming cells and plague forming cells against sheep red blood cells, and so it exhibited that flavonoids inhibited type 2, 3 and 4 hypersensitivity. 3. Quercetin, kaempferol, hesperetin, disodium cromoglycate, malvin and baicalein were active dose-dependently in the all types of hypersensitivity. Fisetin, daidzein, morin, narigin, flavone, catechin, rutin, hesperidin, neophsperidin, apigenin and chrysin were significantly active in the various types of hypersensitivity, but apigenin, rutin and catechin were less active in the delayed hypersensitivity. Taxifolin was significantly active in PCA and histamine-induced anaphylaxis except other types of hypersensitivity. Rotenone and cyanin also inhibited all types of hypersensitivity, but they are toxic. 4. Based on these results from hypersensitivity, the following flavonoid structure-activity relationships became apparent. 1) Flavonoids with $C_{2-3}$ double bond in C-ring were more active than that of $C_{2-3}$ saturation. 2) Flavonoids with $C_4$ ketone group in C-ring were more active than abscence of them except catechin and malvin. 3) Flavonoids with benzene ring at positions 2 or 3 in C-ring exhibited same activities. 4) Flavonoids with opening of the C-ring does not abolish their activities. 5) The glycosylated flavonoids in position 3 or 7 was less active than their aglycone. 6) Flavonoids with the more hydroxy group in A and B-ring were more active. 7) Flavonoids with or without $C_3-OH$ did not change their activities.

• 한방안이비인후피부과학회지
• /
• 제13권1호
• /
• pp.60-77
• /
• 2000

Experimental studies were done to research effects of Leejungtang and Leejungtanggamibang on the anti-allergic effects. We studied the vacular permeability response induced by histamine and serotonin, homologous PCA provoked by IgE-like antibody against EWA, contact dermatitis induced by picryl chloride and delayed type hypersensitivity response to SRBC. The results were obtained as follows: 1. In effects of the Leejungtang and Leejungtanggamibang on the vascular permeability responses to intradermal serotonin, the Leejungtang revealed insignificant effect and Leejungtanggamibang revealed significantly inhibitory effect. 2, In effects of the Leejungtang and Leejungtanggamibang on the vascular permeability responses to intradermal histamine, the Leejungtang revealed insignificant effect and Leejungtanggamibang revealed significantly inhibitory effect. 3. In the homologous PCA provoked by IgE-like antibody against white albumin, the Leejungtang revealed insignificant effect and Leejungtanggamibang revealed significantly inhibitory effect. 4. In the delayed type hypersensitivity responses to picryl chloride, the Leejungtang revealed insignificant effect and Leejungtanggamibang revealed significantly inhibitory effect. 5. In the delayed type hypersensitivity responses to sheep red blood cell the Leejungtang revealed insignificant effect and Leejungtanggamibang revealed significantly inhibitory effect.