• Journal of Physiology & Pathology in Korean Medicine
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• v.35 no.2
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• pp.47-55
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• 2021

In order to ensure the fact that eight principle pattern differentiation is used clinically as a basic guideline for Korean medicine practice, the definition, role and method of yin-yang pattern differentiation with its case report were explored at first. Yinyang Pattern Differentiation is a method of discriminating human tendencies or morbidity based on the yin and yang characteristics expressed in living bodies. And yin and yang are the two contrasting characteristics and aspects of the interaction when certain physical conditions that have a lasting effect on the human physiological metabolic function are correlated with the morbidity. Specific methods of yinyang pattern differentiation can be divided into several types of yin and yang indicators. First, time and space factors like day and night, hot and cold seasons, above and below, topographical districts. Second, colors and pulse and their/or relative clearness and muddiness, hardness and softness, moving and resting. Third, diagnose yin and yang patterns through distinguishing the true and false of a fever and cold in an emergency phase such as increase of brain pressure and shock state. Fourth, general characteristics of the propensity and constitution of a subject such as body type, speech, behavior, and physiological metabolism. And for clinical use, these were summarized again as a symptom indicators of physical signs and color, pulse, tongue and questionnaire indicators of propensity, body type, and space-time characteristics. Conclusively, it was confirmed that yinyang pattern differentiation has its own diagnostic significance which is distinct from exterior-interior, cold-heat and deficiency-excess pattern differentiation.

• Journal of Physiology & Pathology in Korean Medicine
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• v.36 no.5
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• pp.147-154
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• 2022

In order to secure practising rapid pattern(證, zheng) differentiation against acute infectious diseases like corona virus disease-19(COVID-19) showing rapid variation and contagion, a simplified classification of stages centering on the exterior-interior pattern identification with 2 step-subdivision by cold, heat, deficiency, excess pattern and pathogens is proposed. Pattern differentiation by compound patterns of 8 principles is made for the non-severe stage of general cold and the early mild stage of epidemic disease. Compound pattern's names of 8 principles about external infectious diseases are composed of three stages, that is disease site-characters-etiology. Based on early stage symptoms of fever or chilling etc., exterior, interior and half exterior and half interior patterns are determined first, and then cold, heat, deficiency, excess patterns of exterior and interior pattern respectively are determined, and then more concrete differentiation on pathogens of wind, dryness, dampness and dearth of qi, blood, yin, yang accompanied with constitutional and personal illness factors. Summarizing above descriptions, 4 patterns of exterior cold, exterior heat, exterior deficiency, exterior excess and their secondary compound patterns of exterior cold deficiency and exterior cold excess and so on are classified together with treatment method and available decoction for a standard measure of eight principle pattern differentiation.

• The Journal of Pediatrics of Korean Medicine
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• v.32 no.2
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• pp.11-25
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• 2018

Objectives The purpose of this study is to provide evidence of pathogenesis, pattern differentiation, treatment principle, formula of traditional Chinese medicine for autism spectrum disorder by reviewing journal articles published in China. Methods We searched literatures dated up to 8 January, 2018 in China National Knowledge Infrastructure (CNKI), and evaluated pathogenesis, pattern differentiation, treatment principle, formula of traditional Chinese medicine for autism spectrum disorder. Results Thirty four studies were selected for analysis. These studies included pathogenesis, pattern differentiation, treatment principle, formula of traditional Chinese medicine for autism spectrum disorder. The most common pattern differentiations were the pattern of kidney essence depletion, pattern of phlegm confounding the orifices of the heart, pattern of dual vacuity of the heart and spleen, pattern of effulgent heart-liver fire, pattern of liver failing to course freely. The most common treatment principles were supplementing the kidney, transquilizing, fortifying the spleen, transforming phlegm, opening the orifices, and calming the liver. The most commonly-used formulas were Yukmijihwangtang (六味地黃湯), Modified Ondamtang (加味溫膽湯), Guibitang (歸脾湯), Yangsimtang (養心湯), Jwaguihwan (左歸丸), Cheonmagudeungeum (天麻鉤藤飮), and Danchisoyosan (丹梔逍遙散). Conclusions This study shows the latest trend of pathogenesis, pattern differentiation, treatment principle, formula of traditional Chinese medicine for autism spectrum disorder. Further study is needed to solidify these findings.

• International Journal of Oral Biology
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• v.38 no.4
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• pp.161-167
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• 2013

Human dental pulp stem cells (DPSCs) are multi-potent mesenchymal stem cells that have several differentiation potentials. An understanding of thetissues that differentiate from these cells can provide insights for future regenerative therapeutics and tissue engineering strategies. The mesiodens is the most frequent form of supernumerary tooth from which DPSCs can differentiate into several lineages similar to cells from normal deciduous teeth. Recently, it has been shown that nanoscale structures can affect stem cell differentiation. In our presentstudy, we investigated the effects of a 250-nm nanoscale ridge/groove pattern array on the osteogenic and adipogenic differentiation of dental pulp cells from mesiodenscontaining human DPSCs. To this end, the expression of lineage specific markers after differentiation induction was analyzed by lineage specific staining and RT-PCR. The nanoscale pattern arrayed surface showed apositive effect on the adipogenic differentiation of DPSCs. There was no difference between nanoscale pattern arrayed surface and conventional surface groups onosteogenic differentiation. In conclusion, the nanoscale ridge/groove pattern arrayed surface can be used to enhance the adipogenic differentiation of DPSCs derived from mesiodens. This finding provides an improved understanding of the effects of topography on cell differentiation as well as the potential use of supernumerary tooth in regenerative dental medicine.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.2765-2769
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• 2013

Background: A close association between patterns identified by magnifying narrow-band imaging (M-NBI) and histological type has been described. M-NBI patterns were also recently reported to be related to the mucin phenotype; however, detials remain unclear. Materials and Methods: We investigated the cellular differentiation of gastric cancer lesions, along with their mucosal distribution observed by M-NBI. Ninety-seven depressed-type early gastric cancer lesions (74 differentiated and 23 undifferentiated adenocarcinomas) were visualized by M-NBI. Findings were divided into 4 patterns based on abnormal microvascular architecture: a chain loop pattern (CLP), a fine network pattern (FNP), a corkscrew pattern (CSP), and an unclassified pattern. Mucin phenotypes were judged as gastric (G-type), intestinal (I-type), mixed gastric and intestinal (M-type), and null (N-type) based on 4 markers (MAC5AC, MUC6, MUC2, and CD10). The relationship of each pattern of microvascular architecture with organoid differentiation indicated by cancer cell differentiation and its distribution in each histological type of early gastric cancer was investigated. Results: All CLP and FNP lesions were differentiated. The cancer cell distribution showed organoid differentiation in 84.2% (16/19) and 61.1% (22/36) of the two types of lesions, respectively, and there was a significant difference from the unclassified pattern with organoid differentiation (p<0.001). Almost all (94.7%; 18/19) CSP lesions were undifferentiated, and organoid differentiation was observed in 72.2% (13/18). There was a significant difference from the unclassified pattern with organoid differentiation (p<0.05). Conclusions: Cellular differentiation and distribution are associated with microvascular architecture observed by M-NBI.

• Journal of Physiology & Pathology in Korean Medicine
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• v.31 no.1
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• pp.8-19
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• 2017

This study on "Wenbingtiaobian" covers identifying pattern of prescription, understanding system of multiple syndrome differentiations, characteristics of treatment and medicinal substances. The source books are "Korean translation of Wenbingtiaobian", "Modern Shanghanlun", "Jinkuiyaolueyishi", "Chinese Medicine Formulas". "Wenbingtiaobian" has system of multiple patterns including three energizer syndrome differentiation, classification of disease, six meridian syndrome differentiation and wei-qi-ying-xue syndrome differentiation. That describes cause, location, nature, power and transmutation of disease. Wei-qi-ying-xue pattern is meaningful to warm-heat disease and three energizer pattern is relevant to dampness-heat disease. The warm disease shows mostly yang brightness bowel syndrome and patterns of three yin viscera. In aspect of the heat disease, qi aspect pattern makes up the largest number of syndrome differentiation and have sometimes with bowel excess or fluid deficiency. And treatment for wei aspect pattern is primarily 'outthrust the pathogen with pungent-cool'. Deficiency cold pattern and cold pattern with dampness occupy most of cold patterns. And many dampness patterns are dampness-heat pattern in middle energizer and 'inhibited lung qi transforming' is major mechanism. Patterns with fluid deficiency in qi aspect syndrome appear mostly in upper or middle energizer and in xue aspect syndrome appear mostly in lower energizer and they form 20% of all syndrome differentiations. The treatment of clearing heat uses pungent-cool(cold) for upper energizer, sweet-cold for middle energizer, sweet(salty)-cold for lower energizer. The treatment of tonifying yin uses mostly salty-cold for middle or lower energizer. The treatment of outthrusting pathogen is applied to all the wei-qi-ying-xue aspect combined with other treatments by using pungent-cool(cold) and light herbs. Understanding diseases in the respect of syndrome differentiation can enhance understanding of modern diseases from a perspective of Korean Traditional Medicinal(KTM) and can make clinical application of KTM treatments easy. Data from this study are expected to be basic for standardization and systemization of KTM.

• Journal of Korean Medicine for Obesity Research
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• v.8 no.2
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• pp.37-47
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• 2008

Objectives The aim of the study was to investigate the difference between pattern identification of premenopausal(n=39) and climacteric(n=40) korean obese and overweight women using Syndrome Differentiation Questionaire. Methods 39 premenopausal obese women(BMI${\geq}25kg/m^2$) and 40 climacteric overweight and obese women(BMI${\geq}23kg/m^2$) were recruited from October 2007 to March 2008 in Seoul, Korea. Subjects who had other disease were rejected. Basic anthropometry and body composition were measured. Every subjects were given and filled out the Syndrome Differentiation Questionaire, and we analyzed that using Fisher's exact test. Results 1. Premenopausal women showed high frequency of food accumulation pattern(43.6%), but in climacteric women, liver qi depression pattern was frequent(57.5%, p=0.021). 2. In weighted Syndrome Differentiation Questionaire score, Premenopausal women showed high frequency of food accumulation pattern(43.6%), but in climacteric women, liver qi depression pattern was frequent(47.5%, p=0.004). 3. There were no correlation between anthropometry and scores of the each patterns. Conclusions In this study, we can find out that the dietary factors play major roles in obesity of premenopausal women and emotional factors in obese climacteric women in the view of oriental pattern identification diagnosis. But it seemed that there lacked of consideration that reflected the degree of obesity in this Syndrome Differentiation Questionaire.

• Proceedings of the Korean Statistical Society Conference
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• 2005.05a
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• pp.263-269
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• 2005

Users who use Web site wish to get information conveniently. To users who web site operators use Web site differentiation to provide done service pattern analysis by user do must. Association rule is one of data Mining techniques for pattern discovery. If search for pattern by user, differentiation by user done service offer can. Association rule search result that pattern by user can know, and considers web page visiting time for association rule search differentiation done web structure service and recommendation service possible.

• Journal of Physiology & Pathology in Korean Medicine
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• v.31 no.2
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• pp.83-93
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• 2017

This study covers pattern differentiation based on Korean medical references, research trend and modern clinical applications about Sleep-Wake disorders of Diagnostic and Statistical Manual of Mental Disorders(DSM-V) published by American Psychiatric Association. Insomnia disorder is mostly caused by yin deficiency of liver-kidney or liver qi depression and main patterns are heart-kidney non-interaction, deficiency-excess complex pattern containing phlegm-heat due to qi stagnation and blood stasis. Hypersomnolence disorder is more due to yang deficiency rather than yin deficiency and it's major pattern is spleen-kidney yang deficiency. Cataplexy is main feature in narcolepsy and corresponds to depressive psychosis or fainting in terms of Korean Medicine and narcolepsy is assumed to be relevant to liver wind. Breathing-related sleep disorders are related with phlegm-fluid retention brought on spleen deficiency with dampness encumbrance. Pattern of circadian rhythm sleep-wake disorders is combined with yin deficiency of liver-kidney or liver qi depression of insomnia disorder and spleen-kidney yang deficiency or dampness-phlegm of hypersomnolence disorder. Yin deficiency with effulgent fire brought on drugs or alcohol is one of main patterns of substance/medication-induced sleep disorder and combined patterns with yin deficiency of liver-kidney and blood stasis or dampness-phlegm-heat are mostly applied clinically. This study drew major and frequently applied patterns of sleep-wake disorders based on Koran medical literature and modern clinical applications. And that can be the groundwork for the task ahead like clinical practice guideline of sleep-wake disorders containing pattern differentiation, diagnosis and prescriptions.

• Tissue Engineering and Regenerative Medicine
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• v.15 no.6
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• pp.751-760
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• 2018

BACKGROUND: Bone tissue engineering based on pluripotent stem cells (PSCs) is a new approach to deal with bone defects. Protocols have been developed to generate osteoblasts from PSCs. However, the low efficiency of this process is still an important issue that needs to be resolved. Many studies have aimed to improve efficiency, but developing accurate methods to determine efficacy is also critical. Studies using pluripotency to estimate efficacy are rare. Telomerase is highly associated with pluripotency. METHODS: We have described a quantitative method to measure telomerase activity, telomeric repeat elongation assay based on quartz crystal microbalance (QCM). To investigate whether this method could be used to determine the efficiency of in vitro osteogenic differentiation based on pluripotency, we measured the pluripotency pattern of cultures through stemness gene expression, proliferation ability and telomerase activity, measured by QCM. RESULTS: We showed that the pluripotency pattern determined by QCM was similar to the patterns of proliferation ability and gene expression, which showed a slight upregulation at the late stages, within the context of the general downregulation tendency during differentiation. Additionally, a comprehensive gene expression pattern covering nearly every stage of differentiation was identified. CONCLUSION: Therefore, this assay may be powerful tools for determining the efficiency of differentiation systems based on pluripotency. In this study, we not only introduce a new method for determining efficiency based on pluripotency, but also provide more information about the characteristics of osteogenic differentiation which help facilitate future development of more efficient protocols.