• 한국산학기술학회논문지
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• 제11권7호
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• pp.2522-2527
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• 2010

본 연구의 목적은 초음파 처리 키토산과 메일라드 반응액 혼합물에 의한 tyrosinase 억제 활성과 항균력을 조사하고자 하였다. 감자로부터 tyrosinase를 분리하여 SDS-PAGE후 일반 단백질 염색과 tyrosinase 활성 염색을 행하여 tyrosinase를 확인하였다. 여러 가지 당 (glucose, fructose, galactose, xylose, arabinose or ribose)과 cystein과의 반응으로 생성된 메일라드 반응 산물의 tyrosinase 억제 활성을 비교한 결과, glucose과 cystein 반응으로 생성된 메일라드 반응 산물의 tyrosinase 억제 활성이 가장 높은 것으로 나타났다. 0.5% 아세트산에 녹인 1% 키토산을 시간별로 초음파 처리한 후 항균력을 조사한 결과 1 시간 이상 초음파 처리한 경우 E. coli 균주 와 S. aureus 균주에서 억제 활성을 보였다. 갈변 억제 활성과 항균력을 가진 혼합물 제조를 위하여 초음파 처리 키토산과 메일라드 반응액을 혼합비를 달리하여 조사한 결과 부피 비로 1 : 1 로 혼합한 경우가 tyrosinase 억제 활성과 항균력이 가장 효과가 높은 것으로 조사되었다.

• 약학회지
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• 제47권1호
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• pp.20-24
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• 2003

The purpose of this work was to study the effect of extracts from pharbitidis seed and watermelon's inner shell on tyrosinase activity. For this purpose, the effects of plant extracts on total melanin synthesis and tyrosinase activity were measured. The results showed that the extracts effectively inhibited the tyrosinase activity and total melanin synthesis. The non-cytotoxicity of the plant extracts was confirmed by MTT assay.

• 약학회지
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• 제45권5호
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• pp.522-528
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• 2001

Tyrosinase plays an important role in the process of melanin biosynthesis, and it is a biochemical target enzyme for skin-whitening agents and the remedy for disturbances in pigmentation. We have screened 25 natural plant extracts for inhibitory effects against DOPA oxidase activity of tyrosinase. For the inhibitory effect against DOPA oxidase activity of tyrosinase, the recombinant human tyrosinase and the purified mushroom tyrosinase were used. Each of the dried plants extracted with methanol, and then the extracts were subjected to sequential fractionations with methylene chloride, ethyl acetate, n-butanol and water, respective The methylene chloride fractions of Angelica tenuissima, Nardostachys chinensis, Bombyx mop and Saposhnikovia divaricata, and the n-butanol fraction of Bombyx mori notably inhibited the human tyrosinase activity as well as mushroom tyrosinase activity (more than 90% inhibition). This study suggests that the above extracts have a potential as whitening agents as single ingredients or in combination with other of the extracts.

• 한방안이비인후피부과학회지
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• 제16권1호
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• pp.100-111
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• 2003

Recently many efforts were focused to understand the mechanical insights of melanogenesis to develop the agents for hyper-pigmentation and hypo-pigmentation. In the melanin biosynthetic pathway, tyrosinase is the rate limiting enzyme, and ${\alpha}$-melanocyte stimulating hormone(MSH) or cAMP-elevating agents stimulate melanogenesis and enhance the melanin synthesis and the tyrosinase activity. The author has analyzed the effects of Rhizoma Bletillae on the basal melanogenic activities of B16 mouse melanoma cells. Rhizoma Bletillae alone markedly suppressed melanin content and tyrosinase activity in a dose-dependent manner. Pretreatment of the cells with Rhizoma Bletillae. The decrease in the tyrosinase activity was paralled by a decrease in the abundance of tyrosinase protein and tyrosinase promoter activity. These results suggest that Rhizoma Bletillae inhibits melanogenesis of B16 melanoma cells via suppression of tyrosinase activity.

• KSBB Journal
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• 제18권1호
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• pp.45-50
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• 2003

송이의 공시균(KFR1432)과 실험실에서 자체 분리한 균(YxT, WxT, $\textrm{NK}_3\textrm{T}$)의 생장속도의 차이와 FE SEM으로 확인한 미세구조의 차이로 공시균과 자체 분리균이 서로 다른 균임을 확인할 수 있었다. Tyrosinase 활성 억제 실험을 위해 각각 균사체의 열수 추출물, 에탄올 추출물, 배양액을 hexane, chloroform, ethyl acetate로 분획하였다. 그 결과 모든 균의 ethyl acetate 분획층과 수층에서 tyrosinase 활성을 억제하는 것으로 나타났으며, KFR1432의 ethyl acetate 분획층과 YxT의 수층에서 억제율이 100%를 넘어 큰 저해 활성을 보였다. 또한 항산화제 처리 결과 ascorbic acid와 glutathion은 생장을 다소 증가시키거나 별 영향을 주지 못한 반면, tocopherol 의 경우 농도가 높아질수록 생장을 억제하는 것으로 나타났다. Tyrosinase 활성 억제에 있어서는 모든 항산화제 처리구에서 무처리구에 비해 높은 억제율을 보였다.

• 생명과학회지
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• 제16권3호
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• pp.396-399
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• 2006

고구마(품종: 생미)추출액에 tyrosinase의 활성을 저해하는 저해 물질이 존재함을 확인하였으며, 고구마 추출물의 첨가농도를 증가시킬수록 tyrosinase 활성의 저해도가 증가하였다. 그리고 고구마 추출물에 존재하는 tyrosinase 저해제는 $95^{\circ}C$에서 20분간 열처리에 의하여 37.3%의 활성이 잔존하여 열에 비교적 안정하였으며, pH 5.0-7.0에서 가장 안정하였다. 그리고 고구마 추출물은 투석에 의하여 tyrosinase에 대한 저해활성이 유의적으로 감소하였으며, 따라서 고구마 추출물에 존재하는 tyrosinase의 저해활성은 저분자 물질로 추정된다.

• 약학회지
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• 제45권2호
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• pp.214-219
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• 2001

Nineteen hydrolysable tannins isolated from the Euphorbia helioscopia (Euphorbiaceae) were tested for the inhibitory effect on mushroom tyrosinase activity in vitro. Inhibitory effect of gallotannin group exhibited more potent than that of phenolcarboxylic acid and ellagitannin groups against the enzyme activity. The inhibitory activity by pentagalloyl glucose on mushroom tyrosinase was more potent ($IC_{50}$/, 4.9 $\mu$M) than that of kojic acid ($IC_{50}$/, 8.7 $\mu$M).

• 대한화장품학회지
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• 제23권3호
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• pp.115-121
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• 1997

To identify inhibitors of melanogenesis, we compared the effects of 5 compounds on mushroom tyrosinase, human melanocytic tyrosinase activity and melanin content. The cytotoxicyty of the components were also tested on cultured human melanoctes. Kojic acid showed marked inhibitory effect both on mushroom and human tyrosinase activity. This action of kijic acid is stronger than that of ascorbic acid. Arbutin inhibited human tyrosinase activity of cultured melanocytes although it had slightly inhibitory effect on mushroom tyrosinase activity. Azelaic acid had no effect on human tyrosinase activity. Melanin production was inhibited significantly by kojic acid and tranexamic acid. MTT assay showed that all of the compounds were non-cytotoxic to melanocytes at the concentrations tested. These results suggest that the effect of kojic acid on cultured meanocytes involve inhibition of tyrosinase activity and melanogenesis without affection the cell number.

• 동의생리병리학회지
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• 제25권3호
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• pp.528-532
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• 2011

This study was designed to investigate the collagen metabolism and tyrosinase activity of Meretrix extracts (ME). The effect of ME on type I procollagen production and collagenase activity in human normal fibroblasts HS68 after UVB (312 nm) irradiation was measured by ELISA method. The tyrosinase activity after treatment of ME was measured as well. Type I procollagen production was recovered by ME in UVB damaged HS68 cells. The increased collagenase activity after UVB damage was significantly recovered by ME. The tyrosinase activity and L-DOPA oxidation were significantly reduced as well. However, the effects on tyrosinase activity and L-DOPA oxidation were not powerful enough to be used as whitening agents. ME showed the anti-wrinkle effects and some whitening effects in vitro. These results suggest that ME may be a useful drug as an anti-wrinkle treatments.

• 약학회지
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• 제47권6호
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• pp.398-403
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• 2003

To investigate the relationship between structure and biological activity of phenylpropanoids, we measured effects of phenylpropanoids on anti-oxidant and whitening activity, In DPPH radical scavenging activity, caffeic acid analogues showed the significant anti-oxidant activity. Although phenylpropanoids did not inhibit purified-tyrosinase activity, they significantly inhibited tyrosinase activity and melanin production in MSH-stimulated B16 melanoma cells. However, phenylpropanoids did not affect tyrosinase expression in MSH-stimulated B16 melanoma cells, which suggest that inhibition of MSH-induced melanin production was due to tyrosinase inhibition mediated via other signal pathways but not expression of tyrosinase. Phenylpropanoids also significantly inhibited both hyaluronidase and elastase activity, suggesting that phenylpropanoids may be used as whitening, hydration and anti-wrinkling agents. Hydroxyl residue of aromatic ring in phenylpropanoids plays an important role in anti-oxidant and whitening activity.