The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
권호 표지

Sympathetic Regulation of Aquaporin Water Channels in Rat Kidney

  • Lee, Jong-Un (Department of Physiology, Chonnam National University Medical School) ;
  • Yoo, Kwang-Jay (Department of Physiology, Seonam National University College of Medicine) ;
  • Oh, Yoon-Wha (Department of Physiology, Chonnam National University Medical School) ;
  • Lim, Dong-Yoon (Department of Pharmacology, Chosun National University Medical College)
  • Published : 2003.06.21
Download PDF
⟨ Previous Next ⟩

Abstract

Whether there exists a sympathetic neural regulation on the aquaporin (AQP) channels in the kidney was examined. Male Sprague-Dawley rats were used. They were renal nerve denervated by stripping the nervous and connective tissues passing along the renal artery and vein, and painting these vessels with 10% phenol solution through a midline abdominal incision. Three days later, the expression of AQP1-4 proteins in the denervated kidneys was determined. The content of norepinephrine was found significantly decreased following the denervation. Accordingly, the expression of AQP2 proteins was markedly decreased. The expression of AQP3 and AQP4 was also slightly but significantly decreased, while that of AQP1 was not. Neither the basal nor the AVP-stimulated accumulation of cAMP was significantly affected in the denervated kidney. It is suggested that the sympathetic nervous system has a tonic stimulatory effect on AQP channels in the kidney.

Keywords

References

  1. Bar HP: Measurement of adenyl cyclase and cyclic AMP. Methods Pharmacol 3: 593-611, 1975
  2. Bello-Reuss E, Colindres RE, Pastoriza-Munoz E, Mueller RA, Gottschalk CW. Effects of acute unilateral renal denervation in the rat. J Clin Invest 56: 208-217, 1975 https://doi.org/10.1172/JCI108069
  3. Colindres RE, Spielman WS, Moss NG, Harrington WW, Gottschalk CW. Functional evidence for renorenal reflexes in the rat. Am J Physiol 239: 265-270, 1980
  4. Ecelbarger CA, Terris J, Frindt G, Echevarria M, Marples D, Nielsen S, Knepper MA. Aquaporin-3 water channel localization and regulation in rat kidney. Am J Physiol 269: 663-672, 1995
  5. Fushimi K, Uchida S, Hara Y, Hirata Y, Marumo F, Sasaki S. Cloning and expression of apical membrane water channel of rat kidney collecting tubule. Nature 361: 549-552, 1993 https://doi.org/10.1038/361549a0
  6. Gaffney TE, Braunwald E: Importance of the adrenergic nervous system in the support of circulatory function in patients with congestive heart failure. Am J Med 34: 320, 1963 https://doi.org/10.1016/0002-9343(63)90118-9
  7. Gavras H: Role of vasopressin in clinical hypertension and congestive cardiac failure: interaction with the sympathetic nervous system. Clin Chem 37: 1828-1830, 1991
  8. Knepper MA: The aquaporin family of molecular water channels. Proc Natl Acad Sci USA 91: 6255-6258, 1994 https://doi.org/10.1073/pnas.91.14.6255
  9. Kwon TH, Hager H, Nejsum LN, Andersen ML, Frokiaer J, Nielsen S. Physiology and pathophysiology of renal aquaporins. Semin Nephrol 21: 231-238, 2001 https://doi.org/10.1053/snep.2001.21647
  10. Lee J, Oh Y, Kim S, Kim SW. Decreased expression of aquaporin-2 water channels in the kidney in rats treated with reserpine. Kor J Physiol Pharmacol 5: 307-313, 2001
  11. Ma T, Yang B, Gillespie A, Carlson EJ, Epstein CJ, Verkman AS. Severely impaired urinary concentrating ability in transgenic mice lacking aquaporin-1 water channels. J Biol Chem 273: 4296-4299, 1998 https://doi.org/10.1074/jbc.273.8.4296
  12. Nielsen S, Agre P: The aquaporin family of water channels in kidney. Kidney Int 48: 1057-1068, 1995 https://doi.org/10.1038/ki.1995.389
  13. Nielsen S, Chou CL, Marples D, Christensen EI, Kishore BK, Knepper MA. Vasopressin increases water permeability of kidney collecting duct by inducing translocation of aquaporin-CD water channels to plasma membrane. Proc Natl Acad Sci USA 92: 1013-1017, 1995 https://doi.org/10.1073/pnas.92.4.1013
  14. Nielsen S, DiGiovanni SR, Christensen EI, Knepper MA, Harris HW. Cellular and subcellular immunolocalization of vasopressin- regulated water channel in rat kidney. Proc Natl Acad Sci USA 90: 11663-11667, 1993 https://doi.org/10.1073/pnas.90.24.11663
  15. Nielsen S, Terris J, Andersen D, Ecelbarger C, Frokiaer J, Jonassen T, Marples D, Knepper MA, Petersen JS. Congestive heart failure in rats is associated with increased expression and targeting of aquaporin-2 water channel in collecting duct. Proc Nat Acad Sci USA 94: 5450-5455, 1997 https://doi.org/10.1073/pnas.94.10.5450
  16. Nomura G, Kibe Y, Arai S, Uno D, Takeuchi J. Distribution of intrarenal blood flow after renal denervation in the dog. Nephron 16: 126-133, 1976 https://doi.org/10.1159/000180593
  17. Rogenes PR, Gottschalk CW: Renal function in conscious rats with chronic unilateral renal denervation. Am J Physiol 242: 140-148, 1982
  18. Steiner AL, Kipnis DM, Utiger R, Parker C. Radioimmunoassay for the measurement of adenosine 3',5'-cyclic phosphate. Proc Natl Acad Sci USA 64: 367-373, 1969 https://doi.org/10.1073/pnas.64.1.367
  19. Terris J, Ecelbarger CA, Nielsen S, Knepper MA. Long-term regulation of four renal aquaporins in rat. Am J Physiol 271: 414-422, 1996
  20. Xu DL, Martin PY, Ohara M, St John J, Pattison T, Meng X, Morris K, Kim JK, Schrier RW. Upregulation of aquaporin-2 water channel expression in chronic heart failure rat. J Clin Invest 99: 1500-1505, 1997 https://doi.org/10.1172/JCI119312
  21. Yamamoto T, Sasaki S, Fushimi K, Ishibashi K, Yaoita E, Kawasaki K, Marumo F, Kihara I. Vasopressin increases AQP-CD water channel in apical membrane of collecting duct cells in Brattleboro rats. Am J Physiol 268: 1546-1551, 1995 https://doi.org/10.1152/ajpcell.1995.268.6.C1546