Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Cytotoxic Activity of Methanol Fractions and Solvent Extracts from Houttuynia cordata $T_{HUNS}$ (IX) on Various Cancer Cells

어성초 용매추출물과 메탄올 분획물의 암세포주에 대한 세포독성

  • Lee Jeong Ho (Department of Third Medicine, Wonkwang University) ;
  • Baek Seung Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lim Jin A (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Chun Hyun Ja (Division of Natural Science, Wonkwang University) ;
  • Lee Ki Nam (Department of Third Medicine, Wonkwang University)
  • 이정호 (원광대학교 한의학전문대학원 제3의학과) ;
  • 백승화 (한약자원개발학과) ;
  • 임진아 (한약자원개발학과) ;
  • 천현자 (원광대학교 자연과학대학 자연과학기술학부) ;
  • 이기남 (원광대학교 한의학전문대학원 제3의학과)
  • Published : 2003.10.01
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Abstract

This study was carried out to evaluate cytotoxic effects of Houttuynia cordata T/sub HUNB/ extracts on A549 (lung cancer), MDA-MB231 (breast cancer), SNU-C4 (colon cancer) and B16 (mouse melanoma) cell lines. We have determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. The 150 ㎍/㎖ concentration of methanol extract (63.81 %) of Houttuynia cordata T/sub HUNB/ was shown significantly antitoxic activity on A549 cell lines. The order of cytotoxicity fractions of methanol from Houttuynia cordata T/sub HUNB/ extracts against cancer cell lines in vitro is as follows : hexane fraction layer > chloroform fraction layer > ethyl acetate fraction layer > buthanol fraction layer > water fraction layer. These results suggest that the hexane fraction of methanol extract from Houttuynia cordata T/sub HUNB/ extract may be a valuable choice for the development of antitumor agents.

Keywords

References

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